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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor I in Arabidopsis thaliana显示文摘RGF1,分泌的肽荷尔蒙,戏在 Arabidopsis 在根分裂组织开发给角色调音。以前的研究显示功能的 RGF1 需要是在由 tyrosylprotein sulfotransferase 的酷氨酸残余的 sulfated 并且 RGF1 主要经由二个下游的抄写因素调整根分裂组织活动,过多 1 (PLT1 ) 并且 PLT2。然而,细胞外的 RGF1 怎么被一个植物房间察觉,是不清楚的。用基因途径,我们发现了充满白氨酸的重复的 clade 像受体的 kinases,指定了为 RGF1 感觉迟钝 1 (RGI1 ) 到 RGI5,用作 RGF1 的受体。二独立 rgi1 rgi2 rgi3 rgi4 rgi5 五倍的异种与分裂组织的一种小尺寸显示一致的短主要的根显型。四倍的异种显示出的 rgi1 rgi2 rgi3 rgi4 到 RGF1 的显著地减少的敏感,和五倍的异种对 RGF1 完全感觉迟钝。PLT1 和 PLT2 的表示在五倍的异种是几乎无法发现的。一个 RGI2 倡导者极大地在五倍的异种驾驶的 PLT2 的宫外的表示救了它的根分裂组织缺点。RGI 之一, RGI1,随后详细生物化学地被分析。在 vitro 点弄污和下拉,分析显示 RGI1 能身体上与 RGF1 交往。RGF1 的外长的申请能快速并且同时导致 RGI1 的 phosphorylation 和 ubiquitination,显示 RGI1 能察觉并且 transduce RGF1 肽信号。然而,激活的 RGI1 多半很快被翻。这些结果表明那 RGI,充当 RGF1 的受体,在在 Arabidopsis thaliana 的 RGF1-PLT-mediated 根分裂组织开发的戏必需品角色。 | Yang Ou Xiaoting Lu Quaner Zi Qingqing Xun Jingjie Zhang Yujun Wu Hongyong Shi Zhuoyun Wei Baolin Zhao Xiaoyue Zhang Kai He Xiaoping Gou Chuanyou Li Jia Li | 2016 | Cell Research2016，26，6： | 18 |
| 3 | Reservoir accumulation conditions and key exploration&development technologies for Keshen gas field in Tarim Basin显示文摘The Keshen gas field is located in the central part of Kuqa foreland thrust belt in Tarim Basin,and is another large gas field discovered in Kuqa depression after Kela 2 gas field.Since the breakthrough in 2008,a number of large and medium scale gas reservoirs including Keshen 2,Keshen 5 and Keshen 8 have been discovered,that are characterized by ultra depth,ultra-high pressure,ultra-low porosity,ultra-low permeability,high temperature and high pressure.With natural gas geological reserves of nearly trillion cubic meters and production capacity of nearly 5.5 billion cubic meters,the Keshen gas field is the main natural gas producing area in Tarim Oilfield.The Keshen gas field is located in a series of thrusting imbrication structures in the Kelasu structural belt of Kuqa foreland thrust belt.The salt roof structure,plastic rheology of salt beds and pre-salt faulted anticlinal structure constitute the large wedge-shaped thrust body.The thick delta sandstone of the Cretaceous Bashijike Formation is widely distributed,and it forms the superior reservoir-caprock combination with overlying Paleogene thick gypsum-salt bed.The deep Jurassic-Triassic oil and gas migrate vertically along fault system formed in Late Himalaya,break through the thick Cretaceous mudstone and move laterally along the fracture system of the pre-salt reservoirs,to form anticline and fault anticline high pressure reservoir groups.Through near ten years of studies,the three-dimensional seismic acquisition and processing technology for complex mountainous areas,extrusion salt-related structural modeling technology and fractured low-porosity sandstone reservoir evaluation technology have been established,which lay a foundation for realization of oil and gas exploration objectives.Logging acquisition and evaluation technology for high temperature,high pressure,ultra-deep and low-porosity sandstone gas reservoirs,and efficient development technology for fractured tight sandstone gas reservoirs have been developed,which provide a technical support for efficient exploration&development and rapid production of the Keshen gas field. | Haijun Yang Yong Li Yangang Tang Ganglin Lei Xiongwei Sun Peng Zhou Lu Zhou Anming Xu Jingjie Tang Wenhui Zhu Jiangwei Shang Weili Chen Mei Li | 2019 | Petroleum Research2019，4，4： | 3 |
| 4 | Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2-and CDKNIA-interacting protein (BCCIP) in cells显示文摘 | Jiaming Su Yi Sui Jian Ding Fuqiang Li Shuang Shen Yang Yang Zeming Lu Fei Wang Lingling Cao Xiaoxia Liu Jingji Jin Yong Cai | 2016 | Protein & Cell2016，7，10： | 3 |
| 5 | Molecular identification of scallop planktonic larvae using species-specific microsatellites显示文摘The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However,the larvae identification by morphological characteristics is notoriously difficult,mainly due to the small size (usually being less than 150 μm) and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species,the Zhikong scallop Chlamys farreri,the noble scallop C. nobilis,the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers,which was specified by transferability analyses,assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1%~2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple,inexpensive and robust in identification analyses,and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years. | ZHAN Aibin HU Xiaoli BAO Lisui LU Wei PENG Wei WANG Mingling HU Jingjie | 2008 | Acta Oceanologica Sinica2008，27，5： | 3 |
| 6 | An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber(Apostichopus japonicus)显示文摘The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250-700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library constructed in this study consisted of approximately 700 clones. Two hun-dred and thirty-two clones reacted positively after the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site (STMS). | HU Jingjie, ZHAN Aibin, LU Wei, HU Xiaoli, and BAO Zhenmin* Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 2 |
| 7 | Methods Comparison for Microsatellite Marker Development:Different Isolation Methods,Different Yield Efficiency显示文摘Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. | ZHAN Aibin BAO Zhenmin HU Xiaoli LU Wei HU Jingjie | 2009 | Journal of Ocean University of China2009，8，2： | 1 |
| 8 | Towards better water security in North China显示文摘 | Jun Xia Lu Zhang Changming Liu Jingjie Yu | 2007 | Water Resources Management2007，，1： | 1 |
| 9 | Preparation,luminescence and photofunctional performances of a hybrid layered gadolinium-europium hydroxide显示文摘The design and fabrication of rare earth ions incorporated into the inorganic/organic hybrid materials have attracted growing attention for seeking improved optical properties and photofunctional performances.In this paper,a novel hybrid composite based on the layered rare earth hydroxides was successfully prepared by the ion-exchange and intercalation chemical process.The rare earth elements in the composite contain gadolinium(Gd)and europium(Eu)and the molar ratio of Gd to Eu is kept constant at 1.9:0.1.Organic sodium dodecyl sulfonate and dye coumarin-3-carboxyllc acid are simultaneously incorporated into the layered rare earth hydroxides as supporting agent and light-harvesting antenna,respectively.The resulting hybrid layered rare earth hydroxides exhibit the enlarged interlayer distance with about 2.60 nm,and the chemical composition was confirmed through X-ray diffraction,carbon,hydrogen and nitrogen(CHN)elemental analysis,infrared spectroscopy,and thermogravimetric analysis.The layered solid compound shows the characteristic red emission corresponding to the^(5)D_(0)→^(7)F_(2)transition of Eu^(3+)ion,and the luminescence intensity of the optimized compound is greatly enhanced as compared to its corresponding nitrate and the hybrid composite without the introduction of dye molecule.The hybrid layered rare earth hydroxides can be exfoliated into bright colloidal solution,which show superior recognition capability to Cu^(2+)ion with the distinct luminescence quenching.The large quenching constant(1.4×10^(4)L/mol)and low detection limit(0.35μmol/L)are achieved for Cu^(2+)ion,implying a'turn-off'fluorescent sensor for Cu^(2+)detection.Moreover,a transparent film was prepared based on the colloidal solution and displays the typical red emission in folded shape.The new hybrid compound with enhanced luminescence and excellent photofunctional performances is expected to be applied in the fields of fluorescent sensing and flexible optical devices. | Lu Liu Jingjie Yu Shikao Shi Jiye Wang Huihua Song Ruikang Zhang Lianshe Fu | 2022 | Journal of Rare Earths2022，40，9： | 1 |
| 10 | Synthesis of amorphous Pd-based nanocatalysts for efficient alcoholysis of styrene oxide and electrochemical hydrogen evolution显示文摘Amorphous nanomaterials with long-range disordered structures could possess distinct properties and promising applications,especially in catalysis,as compared with their conventional crystalline counterparts.It is imperative to achieve the controlled preparation of amorphous noble metal-based nanomaterials for the exploration of their phase-dependent applications.Here,we report a facile wet-chemical reduction strategy to synthesize various amorphous multimetallic Pd-based nanomaterials,including PdRu,PdRh,and PdRuRh.The phase-dependent catalytic performances of distinct Pd-based nanomaterials towards diverse catalytic applications have been demonstrated.Specifically,the usage of PdRu nanocatalysts with amorphous and crystalline face-centered cubic(fcc)phases can efficiently switch the ring-opening route of styrene oxide to obtain different products with high selectivity through alcoholysis reaction and hydrogenation reaction,respectively.Moreover,when used as an electrocatalyst for hydrogen evolution reaction(HER),the synthesized amorphous PdRh nanocatalyst exhibits low overpotential and high turnover frequency values,outperforming its crystalline fcc counterpart and most of the reported Pd-based HER electrocatalysts. | Yiyao Ge Jingjie Ge Biao Huang Xixi Wang Guigao Liu Xiang-Huan Shan Lu Ma Bo Chen Guanghua Liu Songmo Du An Zhang Hongfei Cheng Qingbo Wa Shiyao Lu Lujiang Li Qinbai Yun Kuo Yuan Qinxin Luo Zhichuan J.Xu Yonghua Du Hua Zhang | 2023 | Nano Research2023，16，4： | 0 |
| 11 | Fluid-Injection-Induced Seismicity Experiment of the WFSD-3P Borehole显示文摘In recent years,the fluid-injection-induced seismicity tests within the WFSD-3P borehole belonging to the Wenchuan Earthquake Fault Science Drilling(WFSD) project,have made remarkable progress.This study has been supported by the Science and Technology Project of China(No.WFSD-00003) | MA Xiumin LI Zhen PENG Hua JIANG Jingjie ZHAO Fang HAN Chaopu YUAN Pengxiang LU Shengzhou PENG Liguo | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 0 |
| 12 | Evolution of the porous structure for phosphoric acid etching carbon as cathodes in Li–O_(2) batteries:Pyrolysis temperature-induced characteristics changes显示文摘Although biomass-derived carbon(biochar)has been widely used in the energy field,the relation between the carbonization condition and the physical/chemical property of the product remains elusive.Here,we revealed the carbonization condition's effect on the morphology,surface property,and electrochemical performance of the obtained carbon.An open slit pore structure with shower-puff-like nanoparticles can be obtained by finely tuning the carbonization temperature,and its unique pore structure and surface properties enable the Li–O_(2) battery with cycling longevity(221 cycles with 99.8%Coulombic efficiency at 0.2 mA cm^(−2) and controlled discharge–charge depths of 500 mAh g^(−1))and high capacity(16,334 mAh g^(−1) at 0.02 mA cm^(−2)).This work provides a greater understanding of the mechanism of the biochar carbonization procedure under various pyrolysis conditions,paving the way for future study of energy storage devices. | Feiyang Yang Ying Yao Yunkai Xu Cong Wang Meiling Wang Jingjie Ren Cunzhong Zhang Feng Wu Jun Lu | 2024 | Carbon Energy2024，6，1： | 0 |
| 13 | Switching CO_(2) Electroreduction Selectivity Between C_(1) and C_(2) Hydrocarbons on Cu Gas-Diffusion Electrodes显示文摘Regulating the selectivity toward a target hydrocarbon product is still the focus of CO_(2)electroreduction.Here,we discover that the original surface Cu species in Cu gas-diffusion electrodes plays a more important role than the surface roughness,local pH,and facet in governing the selectivity toward C_(1)or C_(2)hydrocarbons.The selectivity toward C_(2)H_(4) progressively increases,while CH_(4) decreases steadily upon lowering the Cu oxidation species fraction.At a relatively low electrodeposition voltage of 1.5 V,the Cu gas-diffusion electrode with the highest Cu^(δ+)/Cu^(0)ratio favors the pathways of∗CO hydrogenation to form CH_(4) with maximum Faradaic efficiency of 65.4%and partial current density of 228 mA cm^(−2)at−0.83 V vs RHE.At 2.0 V,the Cu gas-diffusion electrode with the lowest Cu^(δ+)/Cu^(0)ratio prefers C-C coupling to form C_(2)+products with Faradaic efficiency topping 80.1%at−0.75 V vs RHE,where the Faradaic efficiency of C_(2)H_(4) accounts for 46.4%and the partial current density of C_(2)H_(4) achieves 279 mA cm^(−2).This work demonstrates that the selectivity from CH_(4) to C_(2)H_(4) is switchable by tuning surface Cu species composition of Cu gas-diffusion electrodes. | Jianfang Zhang Zhengyuan Li Rui Cai Tianyu Zhang Shize Yang Lu Ma Yan Wang Yucheng Wu Jingjie Wu | 2023 | Energy & Environmental Materials2023，6，2： | 0 |
| 14 | Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene(FcδGST)from Chinese shrimp Fenneropenaeus chinensis显示文摘Glutathione S-transferases(GSTs)are a superfamily of multifunction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates.In the present study,a delta class GST gene(designated as FcδGST)was cloned from Fenneropenaeus chinensis using rapid amplification of c DNA ends(RACE)technology.The complete cDNA sequence of FcδGST was 780 bp in length,which includes a 27-bp 5′non-coding region(UTR),a 117-bp 3′UTR,a 636-bp open reading frame(ORF),and a polyadenylate signal site(AATAAA)presented at the upstream of poly A tail.The FcδGST gene encoded 211 amino acids peptide,including a GST_N domain and a GST_C domain,and exhibited high similarity with previously reported delta GSTs.The predicted molecular mass of FcδGST protein was 23.39 kDa,and its theoretical isoelectric point(pI)was 5.34.The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues,with the highest expression level in hemocytes and hepatopancreas.During the stimulation of Vibrio anguillarum or white spot syndrome virus(WSSV),the m RNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation.The purified recombinant FcδGST protein(designated as rFcδGST)exhibited specific catalytic activity against 1-chloro-2,4-dinitrobenzene(CDNB)substrate with relatively low stable enzymatic activities.These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of F.chinensis. | Jiaobing LI Yan WANG Jingjie HU Yajin XU Qingqian ZHOU Lu ZHANG Mengqiang WANG | 2023 | Journal of Oceanology and Limnology2023，41，5： | 0 |
| 15 | Speaker conversion using kernel non-negative matrix factorization显示文摘Voice conversion(VC) based on Gaussian mixture model(GMM) is the most classic and common method which converts the source spectrum to target spectrum. However this method is prone to over-fitting because of its frame-by-frame conversion. The VC with non-negative matrix factorization(NMF) is presented in this paper, which can keep spectrum from over-fitting by adjusting the size of basis vector(dictionary). In order to realize the non-linear mapping better, kernel NMF(KNMF) is adopted to achieve spectrum mapping. In addition, to increase the accuracy of conversion, KNMF combined with GMM(GKNMF) is also introduced into VC. In the end, KNMF, GKNMF, GMM, principal component regression(PCR), PCR combined with GMM(GPCR), partial least square regression(PLSR), NMF correlation-based frequency warping(NMF-CFW) and deep neural network(DNN) methods are compared with each other. The proposed GKNMF gets better performance in both objective evaluation and subjective evaluation. | Xu Qinyu Lu Guanming Yan Jingjie Li Haibo Cheng Xiao | 2017 | The Journal of China Universities of Posts and Telecommunications2017，24，5： | 0 |
| 16 | MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogels for corneal epithelial healing显示文摘The damage of corneal epithelium may lead to the formation of irreversible corneal opacities and even blindness.The migration rate of corneal epithelial cells directly affects corneal repair.Here,we explored ocu-microRNA 24-3p(miRNA 24-3p)that can promote rabbit corneal epithelial cells migration and cornea repair.Exosomes,an excellent transport carrier,were exacted from adipose derived mesenchymal stem cells for loading with miRNA 24-3p to prepare miRNA 24-3p-rich exosomes(Exos-miRNA 24-3p).It can accelerate corneal epithelial migration in vitro and in vivo.For application in cornea alkali burns,we further modified hyaluronic acid with di(ethylene glycol)monomethyl ether methacrylate(DEGMA)to obtain a thermosensitive hydrogel,also reported a thermosensitive DEGMA-modified hyaluronic acid hydrogel(THH)for the controlled release of Exos-miRNA 24-3p.It formed a highly uniform and clear thin layer on the ocular surface to resist clearance from blinking and extended the drug-ocular-epithelium contact time.The use of THH-3/Exos-miRNA 24-3p for 28 days after alkali burn injury accelerated corneal epithelial defect healing and epithelial maturation.It also reduced corneal stromal fibrosis and macrophage activation.MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogel as a multilevel delivery strategy has a potential use for cell-free therapy of corneal epithelial regeneration. | Xiaomin Sun Wenjing Song Lijing Teng Yongrui Huang Jia Liu Yuehai Peng Xiaoting Lu Jin Yuan Xuan Zhao Qi Zhao Yingni Xu Jingjie Shen Xiaoyun Peng Li Ren | 2023 | Bioactive Materials2023，，7： | 0 |
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