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| 1 | Porphyromonas gingivalis exacerbates ulcerative colitis via Porphyromonas gingivalis peptidylarginine deiminase显示文摘Abstract:Ulcerative Colitis(UC)has been reported to be related to Porphyromonas gingivalis(P.gingivalis).Porphyromonas gingivalis peptidylarginine deiminase(PPAD),a virulence factor released by P.gingivalis,is known to induce inflammatory responses.To explore the pathological relationships between PPAD and UC,we used homologous recombination technology to construct a P.gingivalis strain in which the PPAD gene was deleted(Δppad)and aΔppad strain in which the PPAD gene was restored(comΔppad).C57 BL/6 mice were orally gavaged with saline,P.gingivalis,Δppad,or comΔppad twice a week for the entire 40 days(days 0-40),and then,UC was induced by dextran sodium sulfate(DSS)solution for 10 days(days 31-40).P.gingivalis and comΔppad exacerbated DDS-induced colitis,which was determined by assessing the parameters of colon length,disease activity index,and histological activity index,butΔppad failed to exacerbate DDS-induced colitis.Flow cytometry and ELISA revealed that compared withΔppad,P.gingivalis,and comΔppad increased T helper 17(Th17)cell numbers and interleukin(IL)-17 production but decreased regulatory T cells(Tregs)numbers and IL-10 production in the spleens of mice with UC.We also cocultured P.gingivalis,Δppad,or comΔppad with T lymphocytes in vitro and found that P.gingivalis and comΔppad significantly increased Th17 cell numbers and decreased Treg cell numbers.Immunofluorescence staining of colon tissue paraffin sections also confirmed these results.The results suggested that P.gingivalis exacerbated the severity of UC in part via PPAD. | Xida Zhao Jingbo Liu Chong Zhang Ning Yu Ze Lu Shuwei Zhang Yuchao Li Qian Li Junchao Liu Dongjuan Liu Yaping Pan | 2021 | International Journal of Oral Science2021，13，3： | 5 |
| 2 | The role of cervical mediastinoscopy and video assisted thoracoscopy in the diagnosis and staging of thoraco-mediastinal neoplastic diseases显示文摘 | Attene F Scognamillo F Xidas A | | 0，，01： | 1 |
| 3 | Effect of the type of alkyl ammoniumi- on clay modifier on the structure and thermal/mechanical properties of glassy and rubbery epoxy - clananocomposites 显示文摘 | Xidas P I Triantafyllidis K S | 2010 | European Poly- mer Journal2010，46，： | 1 |
| 4 | Catalytic production of carbon nanotubes over Fe-Ni bimetallic catalysts supported on MgO显示文摘 | Tsoufis T Xidas P Jankovic L | 2007 | Diamond and Related Materials2007，16，1： | 1 |
| 5 | Development of a colorimetric assay for rapid quantitative measurement of clavulanic acid in microbial samples显示文摘We developed a colorimetric assay to quantify clavulanic acid (CA) in culture broth of Streptomyces clavuligerus, to facilitate screening of a large number of S. clavuligerus mutants. The assay is based on a β-lactamase-catalyzed reaction, in which the yellow substrate nitrocefin (λmax =390 nm) is converted to a red product (λmax =486 nm). Since CA can irreversibly inhibit β-lactamase activity, the level of CA in a sample can be measured as a function of the A 390 /A 486 ratio in the assay mixture. The sensitivity and detection window of the assay were determined to be 50 g L-1 and 50 g L-1 to 10 mg L-1 , respectively. The reliability of the assay was confirmed by comparing assay results with those obtained by HPLC. The assay was used to screen a pool of 65 S. clavuligerus mutants and was reliable for identifying CA over-producing mutants. Therefore, the assay saves time and labor in large-scale mutant screening and evaluation tasks. The detection window and the reliability of this assay are markedly better than those of previously reported CA assays. This assay method is suitable for high throughput screening of microbial samples and allows direct visual observation of CA levels on agar plates. | DAI XiDa XIANG SiHai LI Jia GAO Qiang YANG KeQian | 2012 | Science China(Life Sciences)2012，55，2： | 0 |
| 6 | A saccharides sensor developed by symmetrical optical waveguide-based surface plasmon resonance显示文摘We proposed a new saccharides sensor developed by symmetrical optical waveguide(SOW)-based surface plasmon resonance(SPR).This unique MgF_(2)/Au/MgF_(2)/Analyte film structure results in longer suface plasmon wave(SPW)propagation lengths and depths,leading to an increment of resolution.In this paper,we managed to decorate the dielectric interface(MgF_(2) layer)by depositing a thin polydopamine film as surface adherent that provides a platform for secondary reactions with the probe molecule.3-Aminophenylboronic acid(3-PBA)is chosen to be the saccharides sense probe molecule in the present work.The aqueous huumnor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results dermonstrated that this sensor shows great potential in monitoring the blood siugar and can be adapted in the field of biological monitoring in the future. | Ang Li Zhouyi Guo Qing Peng Chan Du Xida Han Le Liu Jun Guo Yonghong He Yanhong Ji | 2015 | Journal of Innovative Optical Health Sciences2015，8，2： | 0 |
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