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| 1 | Outline of Uranium Resources Characteristics and Metallogenetic Regularity in China显示文摘Uranium,as one of twenty-six kinds of important minerals in China,is strategic resource and energy mineral,which has been thoroughly investigated in the project of National Potential Evaluation of Uranium Resource.During the implementation of this project,the authors summarized the metallogenic regularity of uranium resource in China systematically,through the researches of geological characteristics of uranium resource,uranium deposits type(genetic and prediction type),temporal and spatial distribution,and metallogenic series.Based on the investigation of present situation and progress in uranium exploration,this paper proposes the uranium deposits in China should be divided into 4 classes,9 types,21 subtypes in genetic,and 50 types in prediction;suggests to divide China into 29 uranium metallogenic belts and 20 uranium prospective area,and constructs 20uranium-polymetallic metallogenic series,through summarizing temporal and spatial distribution characteristics and metallogenic regularity of uranium deposits in China.The above research is beneficial to the comprehensive understanding of regional uranium metallogenic regularity,and will direct the uranium exploration in the future. | CAI Yuqi ZHANG Jindai LI Ziying GUO Qingyin SONG Jiye FAN Honghai LIU Wusheng QI Fucheng ZHANG Minglin | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 11 |
| 2 | Seasonal variations of stable isotope in precipitation and moisture transport at Yushu,eastern Tibetan Plateau显示文摘Precipitation δ 18O at Yushu, eastern Tibetan Plateau, shows strong fluctuation and lack of clear seasonality. The seasonal pattern of precipitation stable isotope at Yushu is apparently different from either that of the southwest monsoon region to the south or that of the inland region to the north. This different seasonal pattern probably reflects the shift of different moisture sources. In this paper, we present the spatial comparison of the seasonal patterns of precipitation δ 18O, and calculate the moisture transport flux by using the NCAR/NCEP reanalysis data. This allows us to discuss the relation between moisture transport flux and precipitation δ 18O. This study shows that both the southwest monsoon from south and inland air mass transport from north affected the seasonal precipitation δ 18O at Yushu, eastern Tibetan Plateau. Southwest monsoon brings the main part of the moisture, but southwest transport flux is weaker than in the southern part of the Tibetan Plateau. However, contribution of the inland moisture from north or local evaporation moisture is enhanced. The combined effect is the strong fluctuation of summer precipitation δ 18O at Yushu and comparatively poor seasonality. | TIAN LiDe MA LingLong YU WuSheng LIU ZhongFang YIN ChangLiang ZHAO ZhongPing TANG Wei WANG Yu | 2008 | Science China Earth Sciences2008，51，8： | 9 |
| 3 | Temporal and spatial variations of δ^18O in precipitation of the Yarlung Zangbo River Basin显示文摘这份报纸在 2005 在四个车站(Lhaze, Nugesha, Yangcun 和 Nuxia ) 在降水基于未 1 8O 的变化在 Yarlung Zangbo 河盆的降水揭示稳定的同位素的时间、空间的变化。结果证明降水的未 1 8O 在 Yarlung Zangbo 河盆有不同季节的变化。未 1 8O 的更高的价值在季风降水以前在春天发生,并且更低的价值发生在季风降水期间。从空间变化,沿着 Yarlung Zangbo 河山谷在潮湿运输期间与高度效果和 rainout 处理,降水的 18O 逐渐地被弄空。因此,降水的未 1 8O 逐渐地减少从对下游在上游,并且在降水的未 1 8O 的差错率为二个原因是约 0.34 鈥 ? 100m 和 0.7 鈥 ? 100km。在季风降水期间,在降水的未 1 8O 的空间变化被数量效果在大规模统治摘要的状况。 | LIU Zhongfang TIAN Lide YAO Tandong GONG Tongliang YIN Changliang YU Wusheng | 2007 | Journal of Geographical Sciences2007，17，3： | 7 |
| 4 | Co-pyrolysis of bituminous coal and biomass in a pressured fluidized bed显示文摘An experimental study on co-pyrolysis of bituminous coal and biomass was performed in a pressured fluidized bed reactor.The blend ratio of biomass in the mixture was varied between 0 and 100 wt%,and the temperature was over a range of 550–650℃ under 1.0 MPa pressure with different atmospheres.On the basis of the individual pyrolysis behavior of bituminous coal and biomass,the influences of the biomass blending ratio,temperature,pressure and atmosphere on the product distribution were investigated.The results indicated that there existed a synergetic effect in the co-pyrolysis of bituminous coal and biomass in this pressured fluidized bed reactor,especially when the condition of bituminous coal and biomass blend ratio of 70:30(w/w),600℃,and 0.3 MPa was applied.The addition of biomass influenced the tar and char yields and gas and tar composition during co-pyrolysis.The tar yields were higher than the calculated values from individual pyrolysis of each fuel,and consequently the char yields were lower.The experimental results showed that the composition of the gaseous products was not in accordance with those of their individual fuel.The improvement of composition in tar also indicated synergistic effect in the co-pyrolysis. | Yong Huang Ningbo Wang Qiaoxia Liu Wusheng Wang Xiaoxun Ma | 2019 | Chinese Journal of Chemical Engineering2019，27，7： | 7 |
| 5 | Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex显示文摘The nuclear pore complex(NPC)mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells.Here we report the cryo-electron tomography(cryo-ET)structure of the luminal ring(LR)of the NPC from Xenopus laevis oocyte.The observed key structural features of the LR are independently confirmed by single-particle cryo-electron microscopy(cryo-EM)analysis.The LR comprises eight butterfly-shaped subunits,each containing two symmetric wings.Each wing consists of four elongated,tubular protomers.Within the LR subunit,the eight protomers form a Finger domain,which directly contacts the fusion between the inner and outer nuclear membranes and a Grid domain,which serves as a rigid base for the Finger domain.Two neighboring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain,which displays two major conformations and appears to cushion neighboring NPCs.Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC. | Yanqing Zhang Sai Li Chao Zeng Gaoxingyu Huang Xuechen Zhu Qifan Wang Kunpeng Wang Qiang Zhou Chuangye Yan Wusheng Zhang Guangwen Yang Minhao Liu Qinghua Tao Jianlin Lei Yigong Shi | 2020 | Cell Research2020，30，6： | 4 |
| 6 | Structure evolution of Y_2O_3 nanoparticle/Fe composite during mechanical milling and annealing显示文摘Fe-25 wt% Y2O3composite powders have been fabricated by mechanical milling(MM) Fe powders of 100 μm in diameter and Y2O3nanoparticles in an argon atmosphere for the milling periods of4,8,12,24,36,and 48 h,respectively.The features of these powders were characterized by using X-ray diffraction(XRD),scanning electron microscopy(SEM),electron probe micro analyzer(EPMA) and transmission electron microscopy(TEM).The experimental results showed that the mean particle size and crystalline size of MM powders decreased with the milling time increasing.All the elements distributed homogenously inside the powders after 48 h of MM.The lattice constant of the matrix α-Fe kept constant with the milling time,and no solid solution took place during MM process.After 8 h of MM,the α-Fe in each powder became nanocrystalline.After 48 h of MM,Y2O3changes from nanostructure into amorphous structure,and the crystalline size of α-Fe further decreased to 10 nm.The Y2O3in the powders mechanically milled for 48 h kept the amorphous structure after being annealed at 400 1C,and starts to crystallize when the powders are annealed at 600 1C.The amorphous Y2O3contains a small amount of Fe,and crystalline FeYO3appears at 800 1C. | Tong Liu Hailong Shen Chenxi Wang Wusheng Chou | 2013 | Progress in Natural Science:Materials International2013，23，4： | 2 |
| 7 | An optimized protocol for stepwise optimization of real-time RT-PCR analysis显示文摘Computational tool-assisted primer design for real-time reverse transcription(RT)PCR(qPCR)analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.It can lead to false confidence in the quality of the designed primers,which sometimes results in skipping the optimization steps for qPCR.However,the optimization of qPCR parameters plays an essential role in the efficiency,specificity,and sensitivity of each gene’s primers.Here,we proposed an optimized approach to sequentially optimizing primer sequences,annealing temperatures,primer concentrations,and cDNA concentration range for each reference(and target)gene.Our approach started with a sequence-specific primer design that should be based on the single-nucleotide polymorphisms(SNPs)present in all the homologous sequences for each of the reference(and target)genes under study.By combining the efficiency calibrated and standard curve methods with the 2−ΔΔCt method,the standard cDNA concentration curve with a logarithmic scale was obtained for each primer pair for each gene.As a result,an R 2≥0.9999 and the efficiency(E)=100±5% should be achieved for the best primer pair of each gene,which serve as the prerequisite for using the 2^(−ΔΔCt) method for data analysis.We applied our newly developed approach to identify the best reference genes in different tissues and at various inflorescence developmental stages of Tripidium ravennae,an ornamental and biomass grass,and validated their utility under varying abiotic stress conditions.We also applied this approach to test the expression stability of six reference genes in soybean under biotic stress treatment with Xanthomonas axonopodis pv.glycines(Xag).Thus,these case studies demonstrated the effectiveness of our optimized protocol for qPCR analysis. | Fangzhou Zhao Nathan A.Maren Pawel Z.Kosentka Ying-Yu Liao Hongyan Lu James R.Duduit Debao Huang Hamid Ashrafi Tuanjie Zhao Alejandra I.Huerta Thomas G.Ranney Wusheng Liu | 2021 | Horticulture Research2021，8，1： | 2 |
| 8 | Differential SW16.1 allelic effects and genetic backgrounds contributed to increased seed weight after soybean domestication显示文摘Although seed weight has increased following domestication from wild soybean(Glycine soja) to cultivated soybean(Glycine max), the genetic basis underlying this change is unclear. Using mapping populations derived from chromosome segment substitution lines of wild soybean, we identified SW16.1 as the causative gene underlying a major quantitative trait locus controlling seed weight.SW16.1 encodes a nucleus-localized LIM domaincontaining protein. Importantly, the GsSW16.1 allele from wild soybean accession N24852 had a negative effect on seed weight, whereas the GmSW16.1 allele from cultivar NN1138-2 had a positive effect. Gene expression network analysis,reverse-transcription quantitative polymerase chain reaction, and promoter-luciferase reporter transient expression assays suggested that SW16.1 regulates the transcription of MT4, a positive regulator of seed weight. The natural variations in SW16.1 and other known seed weight genes were analyzed in soybean germplasm. The SW16.1 polymorphism was associated with seed weight in 247 soybean accessions, showing much higher frequency of positive-effect alleles in cultivated soybean than in wild soybean. Interestingly,gene allele matrix analysis of the known seed weight genes revealed that G. max has lost 38.5%of the G. soja alleles and that most of the lost alleles had negative effects on seed weight. Our results suggest that eliminating negative alleles from G. soja led to a higher frequency of positive alleles and changed genetic backgrounds in G. max,which contributed to larger seeds in cultivated soybean after domestication from wild soybean.Our findings provide new insights regarding soybean domestication and should assist current soybean breeding programs. | Xianlian Chen Cheng Liu Pengfei Guo Xiaoshuai Hao Yongpeng Pan Kai Zhang Wusheng Liu Lizhi Zhao Wei Luo Jianbo He Yanzhu Su Ting Jin Fenfen Jiang Si Wang Fangdong Liu Rongzhou Xie Changgen Zhen Wei Han Guangnan Xing Wubin Wang Shancen Zhao Yan Li Junyi Gai | 2023 | Journal of Integrative Plant Biology2023，65，7： | 1 |
| 9 | Docetaxel loaded oleic acid-coated hydroxyapatite nanoparticles enhance the docetaxel-induced apoptosis through activation of caspase-2 in androgen independent prostate cancer cells显示文摘 | Yun Luo You Ling Wusheng Guo Jun Pang Weipeng Liu Youqiang Fang Xinqiao Wen Kun Wei Xin Gao | 2010 | Journal of Controlled Release2010，，2： | 1 |
| 10 | Stereotactic Aspiration Plus Subsequent Thrombolysis for Moderate Thalamic Hemorrhage显示文摘 | Maogang Chen Qizhang Wang Wusheng Zhu Qin Yin Minmin Ma Xinying Fan Yongkun Li Guanzhong Ni Chaolai Liu Wenhua Liu Renliang Zhang Gelin Xu Xinfeng Liu | 2012 | World Neurosurgery2012，，1： | 1 |
| 11 | Cadmium can induce alterations in the cellular localization and expression of three major nucleolar proteins in root tip cells of Vicia faba L显示文摘 | Rong Qin Wusheng Jiang Donghua Liu | 2013 | Plant and Soil2013，，1： | 1 |
| 12 | Cadmium accumulation and its effects on metal uptake in maize ( Zea mays L.)显示文摘 | Min Wang Jinhua Zou Xuchuan Duan Wusheng Jiang Donghua Liu | 2005 | Bioresource Technology2005，，1： | 1 |
| 13 | Seasonal deuterium excess in Nagqu precipitation: influence of moisture transport and recycling in the middle of Tibetan Plateau显示文摘 | Zhongfang Liu Lide Tian Tandong Yao Wusheng Yu | 2008 | Environmental Geology2008，，7： | 1 |
| 14 | High-quality conductive polypyrrole within a secondary crosslinked interpenetrating polymer network显示文摘 | Yin Wusheng Liu Changyan Li Jun | 1997 | J Appl Polym Sci1997，65，1： | 1 |
| 15 | The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation显示文摘Compared with most flowers where the showy part comprises specialized leaves(petals)directly subtending the reproductive structures,most Zingiberaceae species produce showy“flowers”through modifications of leaves(bracts)subtending the true flowers throughout an inflorescence.Curcuma alismatifolia,belonging to the Zingiberaceae family,a plant species originating from Southeast Asia,has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different cultivars.Here,we present the chromosome-scale genome assembly of C.alismatifolia“Chiang Mai Pink”and explore the underlying mechanisms of bract pigmentation.Comparative genomic analysis revealed C.alismatifolia contains a residual signal of whole-genome duplication.Duplicated genes,including pigment-related genes,exhibit functional and structural differentiation resulting in diverse bract colors among C.alismatifolia cultivars.In addition,we identified the key genes that produce different colored bracts in C.alismatifolia,such as F3′5'H,DFR,ANS and several transcription factors for anthocyanin synthesis,as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis,bulked segregant analysis using both DNA and RNA data,and population genomic analysis.This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C.alismatifolia and related species.It is also important to understand the variation in the evolution of the Zingiberaceae family. | Xuezhu Liao Yuanjun Ye Xiaoni Zhang Dan Peng Mengmeng Hou Gaofei Fu Jianjun Tan Jianli Zhao Rihong Jiang Yechun Xu Jinmei Liu Jinliang Yang Wusheng Liu Luke RTembrock Genfa Zhu Zhiqiang Wu | 2022 | aBIOTECH2022，3，3： | 1 |
| 16 | Research on the combination model of short-term traffic flow forecasting 显示文摘 | LIU Yuanlin HU Wusheng LI Sulani | 2012 | (178/179/180/181):2668-26722012，，178179180181： | 1 |
| 17 | High-quality conductive polypyrrole within a secondary crosslinked interpenetrating polymer network显示文摘 | Yin Wusheng Liu Changyan Li Jun | 1997 | J Appl Polym Sci1997，65，1： | 1 |
| 18 | Feasibility of treating hyperfibringogenemia with intermittently administered batroxobin in patients with ischemic stroke/transient ischemic attack for secondary prevention 显示文摘 | Gelin Xu Xinfeng Liu Wusheng Zhu | 2007 | Blood Coagulation and Fibrinolysis2007，18，2： | 1 |
| 19 | Study of the effect of preparation method on CuO-ZnO-Al2O3 catalysts 显示文摘 | Ning Wusheng Shen Hangyan Liu Huazhang | 2001 | Appl Catal A: Gen2001，211，： | 1 |
| 20 | Cadmium accumulation, activities of antioxidant enzymes, and malondialdehyde (MDA) content in Pistia stratiotes L.显示文摘 | Yong Li Shanshan Zhang Wusheng Jiang Donghua Liu | 2013 | Environmental Science and Pollution Research2013，，2： | 1 |
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