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2篇 您的检索式:作者名="Workie ANLEY"
    题名 作者 年代 出处 被引量
1Association Mapping of Quantitative Trait Loci for Grain Size in Introgression Line Derived from Oryza rufipogon显示文摘Grain size is one of the critical agronomic traits governing grain yield and quality in rice.However,the underlying genetic mechanisms that control grain size in rice are poorly understood.We used an introgression line derived from Zhonghui 8015 and Oryza rufipogon Griff.This introgression line was evaluated under two different environmental conditions to dissect the quantitative trait loci controlling grain size.Genome-wide association study(GWAS)was performed using 28193 SNPs through a general linear model,and 56 significant SNPs on different loci associated with the 4 grain size traits were detected.Cloned genes including GS3 and q GL3 showed substantial effects on grain length and size.Seven new stable loci were identified with pleiotropic effects on grain size.Haplotype,gene expression analyses,combined gene-based associations,and functional annotations permitted the shortlisting of important dominant genes including GS3 and q GL3.Kashif HUSSAIN ZHANG Yingxing Workie ANLEY Aamir RIAZ Adil ABBAS MdHasanuzzaman RANI WANG Hong SHEN Xihong CAO Liyong CHENG Shihua 2020Rice science2020,27,3:0
2Development of Chromosome Segment Substitution Lines and Genetic Dissection of Grain Size Related Locus in Rice显示文摘To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,respectively.The line CSSL7 was selected for further mapping of qGL1.4/qTGW1.As a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM11824 and RM11842.In F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and TG-57.In addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other traits.In summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.Aamir Riaz Wang Huimin Zhang Zhenhua Workie Anley Zegeye Li Yanhui Wang Hong Xue Pao Peng Zequn Shen Xi hong Cheng Shihua Zhang Yingxin 2021Rice science2021,28,4:0
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