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| 1 | The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility显示文摘Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) cells.Methods: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ kits.Results: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ROS.Conclusions: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy. | Fengjiao Jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang | 2019 | Cancer Biology & Medicine2019，16，1： | 20 |
| 2 | Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds显示文摘最近,我们报导了一条化学途径从老鼠成纤维细胞产生 pluripotent 干细胞。然而,是否化学上导致了 pluripotent 干细胞(CiPSCs ) ,能从要示威的另外的房间类型遗体被导出。用系跟踪,这里,我们首先从成纤维细胞验证 CiPSCs 的产生。下次,我们证明从外胚层的神经干细胞(NSC ) 和从内胚叶的小肠的上皮的房间(IEC ) 能是化学上 reprogrammed 进 pluripotent 干细胞。CiPSCs 源于 NSC 和 IEC 类似于老鼠处于增长率,全球基因表示侧面, epigenetic 地位,自强和区别能力的胚胎的干细胞,和 germline 传播才能。有趣地, pluripotency 基因 Sall4 从不同房间类型,和一样的核心在化学 reprogramming 过程在起始的阶段被表示小分子为 reprogramming 被要求,在从这些多样的房间类型位于化学 reprogramming 下面的分子的机制建议保存。我们的分析也证明这些小分子的使用应该被调整从不同房间类型满足 reprogramming 的要求。一起,这些调查结果证明那条完整的化学 reprogramming 途径能在不同织物起源的房间被使用并且建议化学 reprogramming 是有要延长到更起始的类型的潜力的有希望的策略。 | Junqing Ye Jian Ge Xu Zhang Lin Cheng Zhengyuan Zhang Shan He Yuping Wang Hua Lin Weifeng Yang Junfang Liu Yang Zhao Hongkui Deng | 2016 | Cell Research2016，26，1： | 19 |
| 3 | I-IBV life cycle is restricted in mouse hepatocytes .~xpressing human NTCP显示文摘 | Hanjie Li Qiuyu Zhuang Yuze Wang Tianyi'ng Zhang Jinghua Zhao Yali Zhang Junfang Zhang Yi Lin Quan Yuan Ningshao Xia Jiahuai Han | 2014 | Cellular & Molecular Immunology2014，11，2： | 14 |
| 4 | Feasibility and value of quantitative dynamic contrast enhancement MR imaging in the evaluation of sinonasal tumors显示文摘 | Xian Junfang Du Huarui Wang Xinyan Yan Fei Zhang Zhengyu Hao Hui Zhao Bo Tong Yajian Zhang Jue Han Demin | 2014 | Chinese Medical Journal2014，，12： | 13 |
| 5 | Value of magnetic resonance imaging including dynamic contrast-enhanced magnetic resonance imaging in differentiation between inverted papilloma and malignant tumors in the nasal cavity显示文摘 | Wang Xinyan Zhang Zhengyu Chen Xiaoli Li Jing Xian Junfang | 2014 | Chinese Medical Journal2014，，9： | 13 |
| 6 | Two-photon-excited near-infrared emissive carbon dotsas multifunctional agents for fluorescence imaging andphotothermal therapy显示文摘C dots (CDs) have shown great potential in bioimaging and phototherapy.However, it is challenging to manipulate their fluorescent properties andtherapeutic efficacy to satisfy the requirements for clinic applications. In thisstudy, we prepared S, Se-codoped CDs via a hydrothermal method anddemonstrated that the doping resulted in excitation wavelength-independentnear-infrared (NIR) emissions of the CDs, with peaks at 731 and 820 nm.Significantly, the CDs exhibited a photothermal conversion efficiency of -58.2%,which is the highest reported value for C nanostructures and is comparable tothat of Au nanostructures. Moreover, the CDs had a large two-photon absorptioncross section (-30,045 GM), which allowed NIR emissions and the photothermalconversion of the CDs through the two-photon excitation (TPE) mechanism. Invitro and in vivo tests suggested that CDs can function as new multi functionalphototheranostic agents for the TPE fluorescence imaging and photothermaltheraov of cancer cells. | Minhuan Lan Shaojing Zhao Zhenyu Zhang Li Yan Liang Guo Guangle Niu Jinfeng Zhang Junfang Zhao Hongyan Zhang Pengfei Wang Guangyu Zhu Chun-Sing Lee Wenjun Zhang | 2017 | Nano Research2017，10，9： | 9 |
| 7 | Modulation of Gut Microbiota in Pathological States显示文摘人类微生态是寄居在人体中的微生物聚集体,且主要存在于胃肠道(GIT)中。肠道微生态随着人体发育而演化,并在人类健康和疾病中起着重要作用。近年来,由于微生态会影响宿主代谢、生理学和免疫系统发育,而且微生态紊乱可能导致许多疾病,其越来越受到人们的关注。肠道微生态可能与恶性肿瘤有一定联系,如胃癌和结直肠癌;也可能与其他一些疾病有关,如非酒精性脂肪肝(NAFLD)、被称为工业化世界'生活方式疾病'的肥胖和糖尿病、冠心病以及中枢神经系统紊乱。虽然分子技术革命为我们更准确地研究肠道微生态提供了必要的工具,但是我们需要更精确地阐明其与某些人类疾病病理变化的关系,明确微生态在不同疾病中的作用是新的治疗策略发展的基础。本文概述了肠道微生态对人类健康的重要影响以及调整肠道菌群结构的潜在用途,如菌群移植用于治疗耐药艰难梭菌(C.difficile)的感染。通过微生态干预调整肠道区域以改善人类健康的概念虽刚刚兴起,但其治疗意义显著。因此,抑制有害菌、促进有益菌可能会保护人类健康,并且这些努力将为探索发展更加合理的治疗方案打下基础。 | Yulan Wang BaohongWang Junfang wu xiangyang Jiang Huiru Tang Ole H. Nielsen | 2017 | Engineering2017，3，1： | 9 |
| 8 | Heavy metal(loid) pollution in mine wastes of a Carlin-type gold mine in southwestern Guizhou, China and its environmental impacts显示文摘Mercury pollution resulting from artisanal gold mining is a serious environmental problem in many developing countries. In this study, we analyzed heavy metal(loid) contamination in mine wastes from a Carlin-type gold mine in southwestern Guizhou, China. Highly elevated levels of As, Hg, Tl, Sb, and Cd—up to 5844, 28,29, 581 and 3.0 mg/kg, respectively—were observed in the mine wastes, but no significant accumulation of Cr, Ni, Cu,Zn, and Pb was found. The smelting process of gold ores had significant impacts on the enrichment of As, Tl, and Hg in the mine wastes. Due to the significant metal(loid)pollution in this gold mine, the ecological risks are classified as ‘‘very high.'' Hg and As are the major pollutants with a relative contribution of 55.9 % and 24.2 % to the risk index, respectively. Phytoremediation could be used to reduce heavy metal contamination and recycle the gold simultaneously. Hg–As–Tl pollution should be considered in gold extraction at Carlin-type gold mines. | Ping Li Junfang Zhang Jianxu Wang Zhonggen Li | 2015 | Chinese Journal Of Geochemistry2015，34，3： | 9 |
| 9 | Actin cytoskeleton-dependent pathways for ADMA-induced NF-κB activation and TGF-β high expression in human renal glomerular endothelial cells显示文摘不对称的 dimethylarginine (ADMA ) ,一个内长的氮的氧化物 synthase 禁止者,被认为是在长期的肾疾病(CKD ) 的前进的一个独立风险因素。它能由增加转变生长因素(TGF )-1 表示导致肾纤维变性,但是它的分子的机制是不清楚的。现在的学习的目的是在人的肾的 glomerular endothelial 房间(HRGEC ) 在导致 ADMA 的 TGF-1 高表示调查肌动朊细胞骨架的角色。压力纤维的结构被 immunofluorescence 设想,原子 factor-B (NF-B )DNA 有约束力的活动被 electrophoretic 活动性移动试金估计, TGF-1 表示被西方的污点分析估计。结果证明 ADMA 导致了压力纤维, NF-B 的 DNA 绑定,和 TGF-1 的增加的表示的汇编。当肌动朊细胞骨架的动力学被使解聚肌动朊的代理人 cytochalasin 使不安时, D 和稳定肌动朊的代理人 jasplakinolide,或压力纤维的脱离由 nicotineamide 腺嘌 dinucleotide 磷酸盐 oxidase 禁止者 apocynin 和 p38 绑激活 mitogen 的蛋白质 kinase 禁止者 SB203580, NF-B 的导致 ADMA 的 DNA 绑定和 TGF-1 表示被禁止。这些结果在 HRGEC 在导致 ADMA 的 NF-B 激活和 TGF-1 高表示揭示了肌动朊细胞骨架依赖者机制。肌动朊细胞骨架的特定的指向可以是有用策略在 CKD 阻止激活 ADMA 的肾纤维变性。 | Liyan Wang Dongliang Zhang Junfang Zheng Yiduo Feng Yu Zhang Wenhu Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，11： | 9 |
| 10 | Synthesis,luminescent properties,and theoretical study of novel Sm^(3+) and Dy^(3+) complexes containing β-diketone and 1,10-phenanthroline显示文摘Two new ternary complexes of 1-(2-thienyl)-3-(p-phenylethynylphenyl)-1,3-propanedione(HTPP) and 1,10-phenanthroline(phen) with Sm3+ and Dy3+ were synthesized.The composition of the ternary complexes was characterized as Sm(TPP)3phen and Dy(TPP)3phen,respectively,by infrared(IR) spectra,chemical analysis,elemental analysis,and thermodynamic analysis.At room temperature,under UV light excitation,the Sm3+ and Dy3+ complexes exhibit characteristic emissions of the central ions.It is found that the fluorescence intensity of Sm(TPP)3phen is stronger than that of Dy(TPP)3phen.In order to explain this phenomenon,an accurate quantum chemistry calculation was carried out,and the result is in good agreement with the experiment data. | LIU Xingwang GAO Saishengtai WANG Li SHEN Leijun JIANG Jiadong GAO Junfang | 2011 | Rare Metals2011，30，1： | 7 |
| 11 | Audiological evaluation in Chinese patients with mitochondrial encephalomyopathies显示文摘 | Liu Yuhe Xue Junfang Zhao Danhua Chen Li Yuan Yun Wang Zhaoxia | 2014 | Chinese Medical Journal2014，，12： | 6 |
| 12 | Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis显示文摘An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a 'reporter' gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species. | Junfang Lin Mingyao Zheng Jie Wang Wei Shu Liqiong Guo | 2008 | Progress in Natural Science:Materials International2008，18，7： | 5 |
| 13 | 用高压氧治疗高原脱习服症的效果分析显示文摘目的:探讨用高压氧治疗高原脱习服症的临床效果。方法:对近期结束高原防守任务后赴临潼疗养院进行疗养治疗的31例高原官兵的临床资料进行回顾性研究。我们将这31例患者分为高压氧组和康复疗养组,其中,高压氧组有18例患者,康复疗养组有13例患者。我院对康复疗养组的患者进行康复疗养,在此基础上,对高压氧组的患者进行高压氧治疗。我们在患者入院时、进行治疗后的15天及30天记录其临床症状,并对其临床症状的改善情况进行评分,比较两组患者的评分结果。结果:在治疗后15天后,高压氧组患者的高原脱习服症状评分明显低于康复疗养组的患者,二者相比差异有显著性(P<0.05)。结论:在进行康复疗养的基础上用高压氧治疗高原脱习服症可明显地改善患者的症状。但该方法的治疗效果及成熟的治疗方案尚待进一步的研究。 | 哈振德 Xu Li Shi Runze Guo Guanghui Song Wei Wang Junfang Zhang Quan | 2014 | 当代医药论丛2014，12，14： | 3 |
| 14 | Clinical Implications of Cancer Stem Cell Biology in Hepatocellular Carcinoma显示文摘 | Junfang Ji Xin Wei Wang | 2012 | Seminars in Oncology2012，，4： | 2 |
| 15 | Prediction of thermal conductivity of polymer-based composites by using support vector regression显示文摘Support vector regression (SVR) combined with particle swarm optimization (PSO) for its parameter optimization, was proposed to establish a model to predict the thermal conductivity of polymer-based composites under different mass fractions of fillers (mass fraction of polyethylene (PE) and mass fraction of polystyrene (PS)). The prediction performance of SVR was compared with those of other two theoretical models of spherical packing and flake packing. The result demonstrated that the estimated errors by leave-one-out cross validation (LOOCV) test of SVR models, such as mean absolute error (MAE) and mean absolute percentage error (MAPE), all are smaller than those achieved by the two theoretical models via applying identical samples. It is revealed that the generalization ability of SVR model is superior to those of the two theoretical models. This study suggests that SVR can be used as a powerful approach to foresee the thermal property of polymer-based composites under different mass fractions of polyethylene and polystyrene fillers. | WANG GuiLian CAI CongZhong PEI JunFang ZHU XingJian | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，5： | 2 |
| 16 | A New Growth Model of Fault Attributes in a Strike-Slip Fault System in the Tarim Basin显示文摘Fault attributes generally display a consistent power–law-scaling relationship.Based on new 3 D seismic data,however,we found some exceptional fault attribute relationships of lengths(L)–throw(T)(vertical component of displacement),overlap zone length(Lo)–width(Wo)from a strike-slip fault system of the Ordovician carbonates in the Tarim Basin.The L–T relationship shows two linear segments with breakup at^40 km in fault length.This presents an exceptional throw increase in the second stage,which is attributed to a localization of vertical displacement and deformation in overlapping zones other than the different fault scales in a mature fault zone.The Lo–Wo relationship in the overlapping zones shows multiply stepped-shape patterns,suggesting multiple fault differential growth and periodic increase in fault size.Therefore,we propose a new alternative growth model of fault attributes in strike-slip fault zones,in which the overlapping zones accumulated localized displacement and deformation in the intracratonic strike-slip fault zone. | ZHANG Yintao WU Guanghui WANG Junfang LI Guohui WAN Xiaoguo YANG Tianyi | 2020 | Acta Geologica Sinica(English Edition)2020，94，5： | 1 |
| 17 | Application Status and Prospects of Biogas Fertilizer in Modern Agricultural Production显示文摘On the basis of introducing the nutrient composition and biogas fertilizer,the effects of biogas fertilizer on soil,crops and environment are summarized. Biogas fertilizer can improve soil structure,increase soil organic matter,available nutrient contents and enzyme activity,increase crop yield,quality and resistance,and relieve the non-point source pollution effectively. The harmlessness,application technology and risk are analyzed. Some suggestions are put forward to control the source pollution,strengthen the research of fermentation technology,define the standard of biogas fertilizer,and carry out large and medium-sized biogas engineering. | Jianshuo SHI Junfang YANG Liying WANG Li GUO Yanli REN Ruonan LI Fengzhi ZHAI Xuan SUN | 2018 | Asian Agricultural Research2018，10，7： | 1 |
| 18 | Electronic and elastic properties of LisN under different pressure显示文摘 | Li Wei Chen Junfang Wang Teng | 2010 | Physica B2010，405，1： | 1 |
| 19 | Temporal-spatial distributions of road silt loadings and fugitive road dust emissions in Beijing from 2019 to 2020显示文摘Road silt loading(sL)is an important parameter in the fugitive road dust(FRD)emissions.In this study,the improved Testing Re-entrained Aerosol Kinetic Emissions from Roads(TRAKER)combined with the AP-42 method was firstly developed to quickly measure and estimate the sLs of paved roads in Beijing,China.The annual average sLs in Beijing was 0.59±0.31 g/m^(2)in 2020,and decreased by 22.4%compared with that in 2019.The seasonal variations of sLs followed the order of spring>winter>summer>autumn in the two years.The seasonal mean road sLs on the same type road in the four seasons presented a decline trend from^(2)019 to 2020,especially on the Expressway,decreasing 47.4%-72.7%.The road sLs on the different type roads in the same season followed the order of Major arterial∼Minor arterial∼Branch road>Express road,and Township road∼Country highway>Provincial highway∼National highway.The emission intensities of PM10 and PM_(2.5)from FRD in Beijing in 2020 were lower than those in 2019.The PM10 and PM_(2.5)emission intensities at the four planning areas in the two years all presented the order of the capital functional core area>the urban functional expansion area>the urban development new area>the ecological conservation and development area.The annual emissions of PM10 and PM_(2.5)from FRD in Beijing in 2020 were 74,886 ton and 18,118 ton,respectively,decreasing by∼33.3%compared with those in 2019. | Haibin Wang Lihui Han Tingting Li Song Qu Yuncheng Zhao Shoubin Fan Tong Chen Haoran Cui Junfang Liu | 2023 | Journal of Environmental Sciences2023，，10： | 1 |
| 20 | Sidearm as a Control in the Asymmetric Ring Opening Reaction of Donor-Acceptor Cyclopropane显示文摘A new type of trisoxazoline and bisthiazoline based ligands have been developed,which are absent of chiral motif on the parent skeleton and containa chiral backbone on sidearm.The ligands promote the amine nucleophilic ring opening reaction of 1,1-cyclopropane diesters smoothly,furnishing the γ-amino acid derivatives in high yield with moderate to good enantioselectivity. | Qikai Kang Lijia Wang Zhongbo Zheng Junfang Li Yong Tang | 2014 | Chinese Journal of Chemistry2014，32，8： | 1 |
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