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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | Prevalence of renovascular disease in the elderly: A population-based study显示文摘 | Kimberley J. Hansen Matthew S. Edwards Timothy E. Craven Gregory S. Cherr Sharon A. Jackson Richard G. Appel Gregory L. Burke Richard H. Dean | 2002 | Journal of Vascular Surgery2002，，3： | 1 |
| 3 | 体重调整固定剂量普通肝素与低分子肝素治疗急性静脉血栓栓塞的比较显示文摘背景:普通肝素用于治疗急性静脉血栓栓塞时,通常在监测血凝下静脉输注给药,患者需要住院治疗。然而,体重调整后皮下注射固定剂量普通肝素可能既适用于住院也适用于门诊静脉血栓栓塞患者的治疗。
目的:确定皮下注射体重调整固定剂量普通肝素治疗静脉血栓栓塞是否与低分子肝素同样安全有效。设计、地点及患者:随机、开标、裁定者盲法、非劣效试验。708例急性静脉血栓栓塞患者来自加拿大和新西兰6所大学附属临床中心,年龄≥18岁。试验于1998年9月至2004年2月进行。在随机分组的患者中,11例从疗效分析中排除,8例从安全性分析中排除。
干预:普通肝素皮下注射初始剂量为333U/kg,以后给予固定剂量250U/kg,每12小时1次(n=345)。低分子肝素(依诺肝素或达肝素)按100IU/kg剂量皮下注射,每12小时1次(n=352)。两种治疗均可院外进行,配合华法林治疗3个月。
主要观测指标:随机分组后3个月内复发性静脉血栓栓塞事件及10天内严重出血情况。结果:普通肝素组有13例患者(3.8%)发生静脉血栓栓塞复发,而低分子肝素组有12例患者复发(3.4%;绝对差值,0.4%;95%可信区间,-2.6%~3.3%)。开始治疗后10天内普通肝素组有4例患者(1.1%)发生严重出血,而低分子肝素组有5例(1.4%;绝对差值,-0.3%;95%可信区间,-2.3%~1.7%)。院外治疗患者在普通肝素组占72%,低分子肝素组占68%。
结论:对于急性静脉血栓栓塞患者而言,皮下注射固定剂量普通肝素与低分子肝素一样安全有效,适于门诊治疗。 | Clive Kearon Jeffrey S. Ginsberg Jim A. Julian James Douketis Susan Solymoss Paul Ockelford Sharon Jackson Alexander G. Turpie Betsy MacKinnon Jack Hirsh Michael Gent 王春玲(译) 孙艺红(译) 胡大一(校) | 2007 | 美国医学会杂志(中文版)2007，26，5： | 1 |
| 4 | Prevalence of renovascular disease in the elderly: A population-based study显示文摘 | Kimberley J. Hansen Matthew S. Edwards Timothy E. Craven Gregory S. Cherr Sharon A. Jackson Richard G. Appel Gregory L. Burke Richard H. Dean | 2002 | Journal of Vascular Surgery2002，，3： | 1 |
| 5 | Electrochemical studies of the assorption behavior of serum proteins on titanium 显示文摘 | Douglas R Jackson Sasha Omanovic Sharon G Roscoe | 2000 | Langmiur2000，16，： | 1 |
| 6 | Delirium as a predictor of long-term cognitive impairment in survivors of critical illness显示文摘 | Timothy D. Girard James C. Jackson Pratik P. Pandharipande Brenda T. Pun Jennifer L. Thompson Ayumi K. Shintani Sharon M. Gordon Angelo E. Canonico Robert S. Dittus Gordon R. Bernard E. Wesley Ely | 2010 | Critical Care Medicine2010，，7： | 1 |
| 7 | Cadmium and Prostate Cancer: A Critical Epidemiologic Analysis显示文摘 | Abe E. Sahmoun L. Douglas Case Sharon A. Jackson Gary G. Schwartz | 2005 | Cancer Investigation2005，，： | 1 |
| 8 | Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer显示文摘 | Carla F. Bender Kim Erica L. Jackson Amber E. Woolfenden Sharon Lawrence Imran Babar Sinae Vogel Denise Crowley Roderick T. Bronson Tyler Jacks | 2005 | Cell2005，，6： | 1 |
| 9 | Anti-inflammatory effects of PPAR-γ agonists directly correlate with PPAR-γ expression during acute pancreatitis显示文摘 | Michael D. Rollins M.D. Sharon Sudarshan M.D. Matthew A. Firpo Ph.D. Brooke H. Etherington M.D. Brandon J. Hart M.D. Heidi H. Jackson M.D. Jeffrey D. Jackson M.D. Lyska L. Emerson M.D. David T. Yang M.D. Sean J. Mulvihill M.D. Robert E. Glasgow M.D | 2006 | Journal of Gastrointestinal Surgery2006，，8： | 1 |
| 10 | Serum hepatitis B surface antigen and hepatitis B e antigen titers: Disease phase influences correlation with viral load and intrahepatic hepatitis B virus markers显示文摘 | Alexander J.V. Thompson Tin Nguyen David Iser Anna Ayres Kathy Jackson Margaret Littlejohn John Slavin Scott Bowden Edward J. Gane William Abbott George K.K. Lau Sharon R. Lewin Kumar Visvanathan Paul V. Desmond Stephen A. Locarnini | 2010 | Hepatology2010，，： | 1 |
| 11 | Six-month neuropsychological outcome of medical intensive care unit patients显示文摘 | James C. Jackson Robert P. Hart Sharon M. Gordon Ayumi Shintani Brenda Truman Lisa May E. Wesley Ely | 2003 | Critical Care Medicine2003，，4： | 1 |
| 12 | Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer显示文摘 | Carla F. Bender Kim Erica L. Jackson Amber E. Woolfenden Sharon Lawrence Imran Babar Sinae Vogel Denise Crowley Roderick T. Bronson Tyler Jacks | 2005 | Cell2005，，6： | 1 |
| 13 | Ethnic Differences in Coronary Calcification: The Multi-Ethnic Study of Atherosclerosis (MESA)显示文摘 | Diane E. Bild Robert Detrano Do Peterson Alan Guerci Kiang Liu Eyal Shahar Pamela Ouyang Sharon Jackson Mohammed F. Saad | 2005 | Circulation2005，，10： | 1 |
| 14 | 美国Paradox盆地盐构造新生代的拉伸活化(英文)显示文摘美国科罗拉多高原Paradox盆地中的裂口盐墙和盐背斜,主要是在古生界和中生界沉积物的差异负载作用下发育起来的。盐构造顶部的平缓褶皱,通常被认为是晚白垩纪至古新世拉腊米挤压运动的产物,而其脊顶地堑与山谷,则被认为是挤压后的松弛拉伸及盐溶作用所造成的。基于野外调查、物理模拟以及现代盐构造学理论,研究认为这些平缓褶皱和脊顶地堑,主要是新生代始新世至渐新世北北东向区域拉伸作用的产物。因为岩盐较其围岩软弱,拉伸形变主要集中在早先形成的盐墙、盐背斜及其较薄的顶板中,使盐构造发生活化并形成新的拉伸构造。顶板中的拉伸构造主要包括正断层、地堑、断层滑移(断滑)褶皱以及滚动褶皱。由于区域拉伸方向斜交盐构造走向,断层分布多呈羽列状。在母盐层较厚地区,拉伸使盐刺穿在正断层之下次动上升;而在母盐层较薄地区,拉伸使盐刺穿下降。在初始盐刺穿较高地区,盐构造的侧翼下降;而在初始盐刺穿较低地区,盐构造整体下降。拉伸使盐刺穿变宽,脊顶地堑下沉,形成拉伸型下降盐刺穿特征性的尖角盐体构造。局部地区,盐体从破碎的顶板间溢出,覆盖了下沉的脊顶地堑。 | 戈红星 JACKSON Martin P.A MOSHER Sharon | 2008 | 高校地质学报2008，14，1： | 0 |
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