|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | saRNA-guided Ago2 targets the RITA complex to promoters to stimulate transcription显示文摘 | Victoria Portnoy Szu Hua Sharon Lin Kathy H Li Alma Burlingame Zheng-Hui Hu Hao Li Long-Cheng Li | 2016 | Cell Research2016，26，3： | 6 |
| 2 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 3 | Thrombolysis of stroke mimics via telestroke显示文摘Background The rate of intravenous tissue plasminogen activator(IVtPA)administered to stroke mimics(SM)occurs in 24%-44%of telestroke series.Methods We reviewed 270 suspected acute ischaemic stroke(AIS)patients who were evaluated by telestroke and received IVtPA from 1 July 2016 to 30 September 2017 at our academic comprehensive stroke centre.Results Among 270 AIS patients who received IVtPA via telestroke,64(23.7%)were diagnosed with SM.Compared with patients who had a stroke,the SM group was younger(mean age 56.4 vs 68.2,p<0.0001),more likely to be female(60.9%vs 45.6%,p=0.0324)and had longer door-to needle times(85.3 vs 69.9,p=0.0008).The most common SM diagnoses were migraine 26(40.6%),conversion disorder 12(18.8%),encephalopathy 7(10.9%)and unmasking(9.4%).Among the SM,migraine and conversion disorder were younger compared with the other subgroups(p<0.001).Functional exam elements were noted more frequently in conversion disorder(66.7%)and migraine(34.6%),but rare in other diagnoses(p=0.006).Among the SM,23(35.9%)had a history of a prior similar episodes,and 15(23.4%)had a history of more than 5 spells.Conclusions In our telestroke programme,23.7%of those administered thrombolysis had a final diagnosis of SM. | Vivien H Lee Ravyn Howell Randheer Yadav Sharon Heaton Karen L Wiles Sushil Lakhani | 2022 | Stroke & Vascular Neurology2022，7，3： | 2 |
| 4 | Regulatory challenges for new drugs to treat obesity and eomorbid metabolic disorders 显示文摘 | David J H Jane G Sharon L S | 2009 | Br J ClinPharmaeol2009，68，6： | 1 |
| 5 | Symmetrical modularity of the COP9 signalosome complex suggests its multifunctionality 显示文摘 | SHARON M MAO H STEPHENS E etal | 2009 | Structure2009，17，1： | 1 |
| 6 | Breast cancer in men显示文摘 | Sharon H | 2002 | Annals of Internal Medicine2002，137，8： | 1 |
| 7 | Preventing the Sex-ual Transmission of HIV-1 with Topical Microbicides:Another Piece of the Equation显示文摘 | Lisa C R Sharon L H Charlene S D | 2007 | EDITORIAL COM-MENTARY2007，96，： | 1 |
| 8 | Fungal peritonitis caused by Lecythophora mutabilis显示文摘 | Suhail A Richard JJ Sharon H | 1985 | J Clin Microbiol1985，，22： | 1 |
| 9 | Malignant Self-regard: Accounting forCommonalities in Vulnerably Narcissistic, Depressive, Selfdefeating,and Masochistic Personality Disorders显示文摘 | Steven H Sharon N | 2014 | ComprehensivePsychiatry2014，55，： | 1 |
| 10 | Use of surface electromyography to estimate neck muscle activity显示文摘 | Carolyn M Sharon MB Veerle H | 2000 | J Electromyogr Kinesiol2000，10，： | 1 |
| 11 | Msf-a interacts with hypoxia-inducible factor-1and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis显示文摘 | Sharon A Wang R Matzkin H | 2006 | Cancer Research2006，，66： | 1 |
| 12 | Synthesis and characterization of maleimide and norbornene functionalized benzoxazines显示文摘 | Ishida H Sharon O | | 0，，15： | 1 |
| 13 | Incidence risk of cervical intraepithelial neoplasia 3 or more severe lesions is a function of human papillomavirus genotypes and severity of cytologic al and histological abnormal ities in adult Japanese women显示文摘 | Masayoshi H Hiromasa F Sharon JBH | 2013 | Int J Cancer2013，132，2： | 1 |
| 14 | On the frequency of protein glycosylation,as deduced from analysis of the SWISS-PROT database显示文摘 | Apweiler R Hermjakob H Sharon N | 1999 | Biochim Biophys Acta1999，1473，1： | 1 |
| 15 | Susceptibility of Neisseria meningitidis to 16 Antimicrobial Agents and Characterization of Resistance Mechanisms Affecting Some Agents显示文摘 | James H Jorgensen Sharon A Crawford | 2005 | J Clin Microbiol2005，43，： | 1 |
| 16 | Lectins:Cell-agglutinating and sugar-pecific proteins显示文摘 | Sharon N Lis H | 1972 | Science1972，177，： | 1 |
| 17 | Contracting and Patterns of Nonprofit Governance显示文摘 | SHARON L H | 1998 | Nonprofit management & leadership1998，8，3： | 1 |
| 18 | Experimental Investigation on Dynamic Properties of Rubberized Concrete显示文摘 | ZHENG L SHARON H X YUAN Y | 2008 | Construction and Building Materials2008，22，5： | 1 |
| 19 | Fibroblast growth factor receptor 4 regulates proliferation, anti-apoptosis and alpha-fetoprotein secretion during hepatocellular carcinoma progression and represents a potential target for therapeutic intervention 显示文摘 | Han K H Sharon P Sylvia S | 2008 | Journal of Hepatblogy2008，12，： | 1 |
| 20 | On the frequencyof protein glycosylation, as deduced from analysis of theSWISS-PROT database显示文摘 | ApweilerR Hermjakob H Sharon N | 1999 | Biochim Biopys Acta1999，1473，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员