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| 1 | NICE指南概要:共病状态的临床评估和管理显示文摘大多数慢性疾病患者患有一种或多种慢性疾病,共病状态在老年人中十分常见(图1和图2)。共病状态的重要性在于其与降低生活质量、高死亡率、多重用药、更高药物不良事件几率、更多计划外医疗服务耗费均相关。共病患者需要频繁前往初级医疗保健机构和各个专科就诊,这些机构的医师之间可能缺乏有效沟通交流。 | Caroline Farmer Elisabetta Fenu Norma O'Flynn Bruce Guthrie 符天旭 | 2017 | 中国医院院长2017，13，5： | 8 |
| 2 | Protective action of glutamine in rats with severe acute liver failure显示文摘BACKGROUND Severe acute liver failure(SALF) is a rare, but high-mortality, rapidly evolving syndrome that leads to hepatocyte degeneration with impaired liver function.Thioacetamide(TAA) is a known xenobiotic, which promotes the increase of the formation of reactive oxygen species. Erythroid 2-related factor 2(Nrf2) activates the antioxidant protection of cells. Studies have evidenced the involvement of inflammatory mediators in conditions of oxidative stress.AIM To evaluate the antioxidant effects of glutamine on Nrf2 activation and NFκBmediated inflammation in rats with TAA-induced IHAG.METHODS Male Wistar rats(n = 28) were divided into four groups: control,control+glutamine, TAA, and TAA + glutamine. Two TAA doses(400 mg/kg)were administered intraperitoneally, 8 h apart. Glutamine(25 mg/kg) was administered at 30 min, 24 h, and 36 h. At 48 h, blood was collected for liver integrity analysis [aspartate aminotransferase(AST), alanine aminotransferase(ALT), and alkaline phosphatase(ALP)]. The liver was harvested for histology and assessment of oxidative stress [thiobarbituric acid-reactive substances(TBARS), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), glutathione(GSH), Nrf2, Kelch-like ECH-associated protein 1(Keap1),NADPH quinone oxidoreductase1(NQO1), superoxide dismutase(SOD)] and inflammatory process.RESULTS TAA caused disruption of the hepatic parenchyma, with inflammatoryinfiltration, massive necrosis, and ballooning degeneration. Glutamine mitigated this tissue damage, with visible regeneration of hepatic parenchyma; decreased TBARS(P < 0.001), GSH(P < 0.01), IL-1β, IL6, and TNFα levels(P <0.01) in hepatic tissue; and decreased blood levels of AST, ALT, and ALP(P <0.05). In addition, CAT, GPx, and GST activities were restored in the glutamine group(P<0.01, P <0.01, and P <0.001, respectively vs TAA alone). Glutamine increased expression of Nrf2(P < 0.05), NQO1, and SOD(P < 0.01), as well as levels of IL-10(P <0.001), while decreasing expression of Keap1, TLR4, NFκB(P < 0.001), COX-2 and iNOS,(P < 0.01), and reducing NO_2 and NO_3 levels(P < 0.05).CONCLUSION In the TAA experimental model of IHAG, glutamine activated the Nrf2 pathway,thus promoting antioxidant protection, and blunted the NFκB-mediated pathway, reducing inflammation. | Elizangela G Schemitt Renata M Hartmann Josieli R Colares Francielli Licks Jéferson O Salvi Cláudio A Marroni Norma P Marroni | 2019 | World Journal of Hepatology2019，11，3： | 5 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | Paraneoplastic sensory neuropathy associated with small cell carcinoma of the gallbladder显示文摘 | Norma O Uribe-Uribe Aura Matilde Jimenez-Garduo | 2009 | Annals of Diagnostic Pathology2009，13，2： | 1 |
| 5 | Production, purification, and characterization of a low-molecular-mass xylanase from Aspergillus sp. and its application in baking显示文摘 | Norma A. Camacho Guillermo Aguilar O | 2003 | Applied Biochemistry and Biotechnology2003，，3： | 1 |
| 6 | Bcl-2 protects against oxidative stress while inducing premature senescence显示文摘 | Norma E L o pez-Diazguerrero Hugo L 6 pez-Araiza | 2006 | Free Radical Biology and Medicine2006，40，7： | 1 |
| 7 | Turning on the microscope turret: a new view for the study of brassinosteroid signaling in plant development显示文摘 | Norma Fàbregas Ana I. Ca?o‐Delgado | 2014 | Physiol Plantarum2014，，2： | 1 |
| 8 | Al pha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen显示文摘 | Elizabeth Breininger Norma B Beodegui Cristian M O'Flaherty | 2005 | Theriogenology2005，，63： | 1 |
| 9 | Survivin: A new target for anti-cancer therapy显示文摘 | Bríd M. Ryan Norma O’Donovan Michael J. Duffy | 2009 | Cancer Treatment Reviews2009，，7： | 1 |
| 10 | Spect roelectrochemist ry of palladium hexaeyanoferrate films on platinum substrates 显示文摘 | REYNALDO O L ROBERTO R NORMA R D T | 2003 | Journal of Electroanalytical Chemistry2003，544，： | 1 |
| 11 | Species of agave with antimicrobial activity against selected pathogenic bacteria and fungi显示文摘 | ANGELES V S JULIA V SANTOS G NORMA H AZUCENA O CATALINA R | 2008 | World J Microbiol Biotcchnol2008，24，： | 1 |
| 12 | Trans Fatty Acids Induce Vascular Inflammation andReduce Vascular Nitric Oxide Production in Endothe-lial Cells显示文摘 | Naomi G Iwata Matilda Pham Norma O Rizzo | 2011 | PLoS ONE2011，6，29： | 1 |
| 13 | Adsorption isotherm studies of chromium (VI) from aqueous solutions using sol-gel hydrotalcite-like compounds 显示文摘 | ESTHELA R R NORMA L GUTIERREZ O | 2009 | J Hazard Mater2009，172，： | 1 |
| 14 | Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen显示文摘 | Elizabeth Breininger Norma B. Beorlegui Cristián M. O’Flaherty Martha T. Beconi | 2004 | Theriogenology2004，，8： | 1 |
| 15 | EGFR and HER2 inhibition in pancreatic cancer显示文摘 | Naomi Walsh Susan Kennedy AnneMarie Larkin Brendan Corkery Lorraine O’Driscoll Martin Clynes John Crown Norma O’Donovan | 2013 | Investigational New Drugs2013，，3： | 1 |
| 16 | Use of molecular markers for predicting therapy response in cancer patients显示文摘 | Michael J. Duffy Norma O’Donovan John Crown | 2010 | Cancer Treatment Reviews2010，，2： | 1 |
| 17 | Survivin: A promising tumor biomarker显示文摘 | Michael J. Duffy Norma O’Donovan Donal J. Brennan William M. Gallagher Bríd M. Ryan | 2006 | Cancer Letters2006，，1： | 1 |
| 18 | Effect of different β-glucans on the respiratory burst of turbot (Psetta maxima) and gilthead seabream (Sparus aurata) phagocytes显示文摘 | Rosario C Norma C Alex O | 1999 | Fish & Shellfish Immunology1999，，9： | 1 |
| 19 | Survivin: A new target for anti-cancer therapy显示文摘 | Bríd M. Ryan Norma O’Donovan Michael J. Duffy | 2009 | Cancer Treatment Reviews2009，，7： | 1 |
| 20 | NICE指南概要:共病状态的临床评估和管理显示文摘大多数慢性疾病患者患有一种或多种慢性疾病,共病状态在老年人中十分常见(见信息图1和框图1)。共病状态的重要性在于其与降低生活质量、高死亡率、多重用药、更高药物不良事件几率、更多计划外医疗服务耗费均相关。 | Caroline Farmer Elisabetta Fenu Norma O'Flynn Bruce Guthrie 符天旭 周国鹏 | 2017 | 英国医学杂志中文版2017，20，1： | 0 |
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