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| 1 | Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System显示文摘 | Mugui Wang Yanfei Mao Yuming Lu Xiaoping Tao Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 56 |
| 2 | Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System显示文摘 | Mugui Wang Yuming Lu Jose Ramon Botella Yanfei Mao Kai Hua Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 34 |
| 3 | Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems显示文摘We developed simplified single transcriptional unit(SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its cr RNA array are co-expressed from a single Pol Ⅱ promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing. | Mugui Wang Yanfei Mao Yuming Lu Zhidan Wang Xiaoping Tao Jian-Kang Zhu | 2018 | Journal of Integrative Plant Biology2018，60，8： | 21 |
| 4 | Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants显示文摘Base editing technologies enable precise base alterations in a target gene without inducing double-stranded breaks,and thus are powerful for targeted gene evolution in vivo(Zhan et al.,2021).The newly evolved cytosine deaminases such as evoAPOBEC1,evoFERNY and evoCDA1,adenine deaminase TadA8e,and a near-protospacer adjacent motif(PAM)less SpRYCas9 variant,have greatly increased base editing efficiency and expanded the editing window in plants(Zhang et al.,2021;Xu et al.,2021c;Tan et al.,2022).Dual cytosine and adenine editors enable concurrent C-to-T and A-to-G conversions and thus enrich the editing outcome.Although plant dual editors have been developed(Li et al.,2020;Xu et al.,2021a),they are rarely applied in evolving plant genes due to low efficiencies. | Hongzhi Wang Yuxin He Yingying Wang Zuren Li Jiannan Hao Yijiao Song Mugui Wang Jian-Kang Zhu | 2022 | Journal of Integrative Plant Biology2022，64，11： | 2 |
| 5 | SWO1 modulates cell wall integrity under salt stress by interacting with importinɑin Arabidopsis显示文摘Maintenance of cell wall integrity is of great importance not only for plant growth and development,but also for the adaptation of plants to adverse environments.However,how the cell wall integrity is modulated under salt stress is still poorly understood.Here,we report that a nuclear-localized Agenet domain-containing protein SWO1(SWOLLEN 1)is required for the maintenance of cell wall integrity in Arabidopsis under salt stress.Mutation in SWO1 gene results in swollen root tips,disordered root cell morphology,and root elongation inhibition under salt stress.The swo1 mutant accumulates less cellulose and pectin but more lignin under high salinity.RNA-seq and ChIP-seq assays reveal that SWO1 binds to the promoter of several cell wall-related genes and regulates their expression under saline conditions.Further study indicates that SWO1 interacts with importinɑIMPA1 and IMPA2,which are required for the import of nuclear-localized proteins.The impa1 impa2 double mutant also exhibits root growth inhibition under salt stress and mutations of these two genes aggravate the salt-hypersensitive phenotype of the swo1 mutant.Taken together,our data suggest that SWO1 functions together with importinɑto regulate the expression of cell wall-related genes,which enables plants to maintain cell wall integrity under high salinity. | Zhidan Wang Mugui Wang Changhong Yang Lun Zhao Guochen Qin Li Peng Qijie Zheng Wenfeng Nie Chun-Peng Song Huazhong Shi Jian-Kang Zhu Chunzhao Zhao | 2021 | Stress Biology2021，1，1： | 0 |
| 6 | Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture显示文摘Of the more than 370000 species of higher plants in nature,fewer than 0.1%can be geneticallymodified due to limitations of the current gene delivery systems.Even for those that can be genetically modified,the modification involves a tedious and costly tissue culture process.Here,we describe an extremely simple cut-dip-budding(CDB)delivery system,which uses Agrobacterium rhizogene to inoculate explants,generating transformed roots that produce transformed buds due to root suckering.We have successfully used CDB to achieve the heritable transformation of plant species inmultiple plant families,including two herbaceous plants(Taraxacum kok-saghyz and Coronilla varia),a tuberous root plant(sweet potato),and three woody plant species(Ailanthus altissima,Aralia elata,and Clerodendrum chinense).These plants have previously been difficult or impossible to transform,but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol,under non-sterile conditions and without the need for tissue culture.Our work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method. | Xuesong Cao Hongtao Xie Minglei Song Jinghua Lu Ping Ma Boyu Huang Mugui Wang Yifu Tian Fan Chen Jun Peng Zhaobo Lang Guofu Li Jian-Kang Zhu | 2023 | The Innovation2023，4，1： | 0 |
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