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| 1 | 鸡DMRT1基因CRISPR/Cas9载体构建及打靶效率的检测显示文摘DMRT1(Double sex and Mab-3 related transcription factor 1)是鸡性腺分化和发育的关键候选基因,其详细的功能和作用途径还未被阐明。为了解明DMRT1在鸡性腺分化和发育过程中的功能,研究首次利用在线sg RNA平台设计4条鸡DMRT1 sg RNA序列,构建了4个sg RNA表达载体:Guide-1、Guide-2、Guide-3和Guide-4。转染鸡DF-1成纤维细胞后,利用T7E1内切酶的方法检测4个表达载体的打靶效率。结果显示,成功构建了4条正确的鸡DMRT1 sg RNA表达载体,并且都能高效地在DF-1细胞中表达,其中Guide-4表达载体能有效地打靶DMRT1基因,打靶效率为24.82%。研究结果为进一步在鸡胚敲除DMRT1基因,研究其功能奠定了基础。 | 杨秀荣 邓继贤 赵德彪 Mike McGrew Sunil Nandi 蒋和生 Michael Clinton | 2017 | 中国家禽2017，39，2： | 9 |
| 2 | Fusobacterium 's link to colorectal neoplasia sequenced: A systematic review and future insights显示文摘AIM To critically evaluate previous scientific evidence on Fusobacterium's role in colorectal neoplasia development.METHODS Two independent investigators systematically reviewed all original scientific articles published between January,2000,and July,2017,using Pub Med,EMBASE,and MEDLINE. A total of 355 articles were screened at the abstract level. Of these,only original scientific human,animal,and in vitro studies investigating Fusobacterium and its relationship with colorectal cancer(CRC) were included in the analysis. Abstracts,review articles,studies investigating other colonic diseases,and studies written in other languages than English were excluded from our analysis. Ninety articles were included after removing duplicates,resolving disagreements between the two reviewers,and applying the above criteria.RESULTS Studies have consistently identified positive associations between Fusobacterium,especially Fusobacterium nucleatum(F. nucleatum),and CRC. Stronger associations were seen in CRCs proximal to the splenic flexure and Cp G island methylator phenotype(CIMP)-high CRCs. There was evidence of temporality and a biological gradient,with increased F. nucleatum DNA detection and quantity along the traditional adenoma-carcinoma sequence and in CIMP-high CRC precursors. Diet may have a differential impact on colonic F. nucleatum enrichment;evidence suggests that high fiber diet may reduce the risk of a subset of CRCs that are F. nucleatum DNA-positive. Data also suggest shorter CRC and disease-specific survival with increased amount of F. nucleatum DNA in CRC tissue. The pathophysiology of enrichment of F. nucleatum and other Fusobacterium species in colonic tissue is unclear;however,the virulence factors and changes to the local colonic environment with disruption of the protective mucus layer may contribute. The presence of a host lectin(Gal-Gal NAc) in the colonic epithelium may also mediate F. nucleatum attachment to CRC and precursors through interaction with an F. nucleatum protein,fibroblast activation protein 2(FAP2). The clinical significance of detection or enrichment of Fusobacterium in colorectal neoplasia is ambiguous,but data suggest a procarcinogenic effect of F. nucleatum,likely due to activation of oncogenic and inflammatory pathways and modulation of the tumor immune environment. This is hypothesized to be mediated by certain F. nucleatum strains carrying invasive properties and virulence factors such as Fad A and FAP.CONCLUSION Evidence suggests a potential active role of Fusobacterium,specifically F. nucleatum,in CRC. Future prospective and experimental human studies would fill an important gap in this literature. | Hisham Hussan Steven K Clinton Kristen Roberts Michael T Bailey | 2017 | World Journal of Gastroenterology2017，23，48： | 9 |
| 3 | Cointegration analysis of metals futures显示文摘 | Clinton W and Michael M | 2002 | Mathematics and Computers in Simulation2002，，59： | 1 |
| 4 | Cointegration analysis of metMs futures 显示文摘 | Clintons W Michael M | 2002 | Mathematics and computers in simulation2002，59，13： | 1 |
| 5 | Above-ground biomass accumulation and nitrogen fixation of broom ( Cytisus scoparius L.) growing with juvenile Pinus radiata on a dryland site显示文摘 | Michael S. Watt Peter W. Clinton David Whitehead Brian Richardson Euan G. Mason Alan C. Leckie | 2003 | Forest Ecology and Management2003，，1： | 1 |
| 6 | PPAR[gamma]1 as a Molecular Target of Eicosapentaenoic Acid in Human Colon Cancer (HT-29) Cells1,2显示文摘 | Allred Clinton D Talbert Dominique R Southard R Chase Wang Xin Kilgore Michael W | 2008 | The Journal of Nutrition2008，，2： | 1 |
| 7 | Timing of Multimodality Therapy for Resectable Synchronous Colorectal Liver Metastases: A Retrospective Multi-Institutional Analysis显示文摘 | Srinevas K. Reddy Daria Zorzi Ying Wei Lum Andrew S. Barbas Timothy M. Pawlik Dario Ribero Eddie K. Abdalla Michael A. Choti Clinton Kemp Jean-Nicolas Vauthey Michael A. Morse Rebekah R. White Bryan M. Clary | 2009 | Annals of Surgical Oncology2009，，7： | 1 |
| 8 | Co-integra-tion Analysis of Metals Futures显示文摘 | Clinton Watkins Michael Mcaleer | | 0，，08： | 1 |
| 9 | Lymphoid populations of gut mucosa in chickens显示文摘 | Dr. Franck Arnaud-Battandier MD Dr. E. Clinton Lawrence MD R. Michael Blaese MD | 1980 | Digestive Diseases and Sciences1980，，4： | 1 |
| 10 | Electricalconduction in exploded segmented wires 显示文摘 | Clinton E H John D P Michael J K | 1998 | Journal ofApplied Physics1998，84，9： | 1 |
| 11 | Design and characterization of ebolavirus GP prehairpin intermediate mimics as drug targets显示文摘 | Tracy R. Clinton Matthew T. Weinstock Michael T. Jacobsen Nicolas Szabo‐Fresnais Maya J. Pandya Frank G. Whitby Andrew S. Herbert Laura I. Prugar Rena McKinnon Christopher P. Hill Brett D. Welch John M. Dye Debra M. Eckert Michael S. Kay | 2015 | Protein Science2015，，： | 1 |
| 12 | Cointegration analysis of metal futures显示文摘 | Clinton W and Michael M | 2002 | Mathematics and Computers in Simulation2002，，59： | 1 |
| 13 | Electrical Conduction in Exploded Segmented Wires显示文摘 | Clinton E H John D P Michael J K | | 0，，09： | 1 |
| 14 | Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation显示文摘 | Pasqualino Loi Michael Clinton Barbara Barboni | 2002 | Biology of Reproduction2002，67，： | 1 |
| 15 | A Grounded Theory of Portfolio Working: Experiencing the Smallest of Small Business显示文摘 | Michael Clinton Peter Totterdell Stephen Wood | 2006 | International Small Business Journal2006，24，2： | 1 |
| 16 | Cointegration analysis of metals futures显示文摘 | Michael M | 2002 | Mathematics and computers in simulation2002，59，： | 1 |
| 17 | Cointegration analysis of metals futures显示文摘 | Michael M | 2002 | Mathematics and Computers in Simulation2002，59，： | 1 |
| 18 | Electrical conduction in exploded segmen- ted wires显示文摘 | Clinton E Hollandsworth John D Powell Michael J Keele | 1998 | Journal of Applied Physics1998，84，9： | 1 |
| 19 | 鸡W染色体基因GPBP1在胚胎组织中的表达研究显示文摘鸡W染色体为进化过程中保留下来的Z染色体高度退化后的拷贝,在机体发育和代谢等过程中发挥着重要作用。以鸡W染色体上富含GC启动子的结合蛋白(GPBP1)基因为对象,对其在胚胎发育过程中的表达和功能进行初步研究;根据GPBP1基因在W和Z染色体上序列的不同,设计特异性引物扩增其W拷贝(GPBP1-W)、Z拷贝(GPBP1-Z)及同时扩增W和Z拷贝(GPBP1-ZW),采用qPCR及Western blot技术分析其在胚胎发育过程中不同组织的表达量,并通过FAD诱导性反转探究其对性别表型的响应。结果表明:GPBP1-W在孵化6 d的胚胎性腺中高表达,且表达量随胚胎发育而升高。GPBP1-W和GPBP1-Z在所检测的孵化12 d的胚胎组织中均有表达,其中mRNA高表达于脾脏组织中,蛋白高表达于性腺组织中;GPBP1-Z mRNA和蛋白质在雄性组织中的表达量高于雌性。GPBP1-W表达受雌性向雄性性反转的影响而显著降低,但GPBP1-Z的表达不受性反转的影响。这一研究提示,鸡性染色体基因GPBP1在鸡胚的性腺和脾脏中表达量高,且其Z拷贝在性别间和各组织中存在剂量效应和转录后调控作用,而W拷贝可能在雌性的部分组织中作为Z拷贝的补偿以保证其生物学功能的发挥。 | 林晓 薛颖 刘龙 樊艳凤 高玉时 赵德标 Clinton Michael 龚道清 | 2021 | 扬州大学学报(农业与生命科学版)2021，42，2： | 0 |
| 20 | Connecting the Dots:A New and Complete Salicylic Acid Biosynthesis Pathway显示文摘Salicylic acid(SA) or 2-hydroxybenzoic acid,better known as the active ingredient in aspirin,is a phenolic plant hormone that plays an essential role in plant defense against biotrophic and semi-biotrophic pathogens(Fu and Dong,2013). | Huan Chen Michael Clinton Guang Qi Daowen Wang Fengquan Liu Zheng Qing Fu | 2019 | Molecular Plant2019，12，12： | 0 |
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