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| 1 | Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model显示文摘AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine. | Florian Graepler Marie-Luise Lemken Wolfgang A Wybranietz Ulrike Schmidt Irina Smirnow Christine D GroB Martin Spiegel Andrea Schenk Schenk Hansj(o|¨)rg Graf Ulrike A Lauer Reinhard Vonthein Michael Gregor Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，44： | 2 |
| 2 | Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA显示文摘 | Maren Kuhne Martin Dippong Sabine Flemig Katrin Hoffmann Kristin Petsch J?rg A. Schenk Hans-J?rg Kunte Rudolf J. Schneider | 2014 | Journal of Immunological Methods2014，，： | 1 |
| 3 | Randomized trial on endoscopic resection-cap versus multiband mucosectomy for piecemeal endoscopic resection of early Barrett’s neoplasia显示文摘 | Roos E. Pouw Frederike G.I. van Vilsteren Femke P. Peters Lorenza Alvarez Herrero Fiebo J.W. ten Kate Mike Visser Boudewijn E. Schenk Erik J. Schoon Frans T.M. Peters Martin Houben Raf Bisschops Bas L.A.M. Weusten Jacques J.G.H.M. Bergman | 2011 | Gastrointestinal Endoscopy2011，，： | 1 |
| 4 | Contributors to individual quality of life after liver transplantation显示文摘 | Christian Thiel Kerstin Landgrebe Evelyn Knubben Silvio Nadalin Ruth Ladurner Christian Grasshoff Alfred K?nigsrainer Martin Schenk Karolin Thiel | 2012 | European Journal of Clinical Investigation2012，，1： | 1 |
| 5 | Porcine model characterizing various parameters assessing the outcome after acetaminophen intoxication induced acute liver failure显示文摘AIM To investigate the changes of hemodynamic and laboratory parameters during the course of acute liver failure following acetaminophen overdose.METHODS Eight pigs underwent a midline laparotomy following jejunal catheter placement for further acetaminophen intoxication and positioning of a portal vein Doppler flow-probe. Acute liver failure was realized by intrajejunal acetaminophen administration in six animals, two animals were sham operated. All animals were invasively monitored and received standardized intensive care support throughout the study. Portal blood flow, hemodynamic and ventilation parameters were continuously recorded. Laboratory parameters were analysed every eight hours. Liver biopsies were sampled every 24 h following intoxication and upon autopsy.RESULTS Acute liver failure (ALF) occurred after 28 ± 5 h resulted in multiple organ failure and death despite maximal support after further 21 ± 1 h (study end). Portal blood flow (baseline 1100 ± 156 m L/min) increased to a maximum flow of 1873 ± 175 m L/min at manifestation of ALF, which was significantly elevated(P < 0.01). Immediately after peaking, portal flow declined rapidly to 283 ± 135 m L/min at study end. Thrombocyte values (baseline 307 × 103/μL± 34 × 103/μL) of intoxicated animals declined slowly to values of 145 × 103/μL± 46 × 103/μL when liver failure occurred. Subsequent appearance of severe thrombocytopenia in liver failure resulted in values of 11 × 103/μL± 3 × 103/μL preceding fatality within few hours which was significant(P > 0.01).CONCLUSION Declining portal blood flow and subsequent severe thrombocytopenia after acetaminophen intoxication precede fatality in a porcine acute liver failure model. | Karolin Thiel Wilfried Klingert Kathrin Klingert Matthias H Morgalla Martin U Schuhmann Pamela Leckie Yalda Sharifi Nathan A Davies Rajiv Jalan Andreas Peter Christian Grasshoff Alfred Konigsrainer Martin Schenk Christian Thiel | 2017 | World Journal of Gastroenterology2017，23，9： | 1 |
| 6 | Combined Oxidative and bio- logical treatment for separated streams of tannery wastewater显示文摘 | Jan C Jochimsen Harm Schenk Martin R Jekel | 1997 | Water science and technolo-1997，36，2： | 1 |
| 7 | Randomized controlled study of EMR versus endoscopic submucosal dissection with a water-jet hybrid-knife of esophageal lesions in a porcine model显示文摘 | Horst Neuhaus Katja Wirths Martin Schenk Markus Dominik Enderle Brigitte Schumacher | 2009 | Gastrointestinal Endoscopy2009，，1： | 1 |
| 8 | FITC-linked Fibrin-Binding Peptide and real-time live confocal microscopy as a novel tool to visualize fibrin(ogen)in coagulation显示文摘Background and Aim:Although fibrinogen has been established as a key player in the process of coagulation,many questions about the role of fibrinogen under specific conditions remain.Confocal microscopic assessment of clot formation,and in particular the role that fibrinogen plays in this process,is commonly investigated using pre-labeled fibrinogen.This has a number of drawbacks,primarily that it is impossible to stain fibrin networks after their formation.The aim of the present study is to present an alternative for conveniently post-staining fibrin in a wide range of models/situations,in real time and with high resolution.Methods:We combined a peptide known to bind fibrin and linked it to fluorescein isothiocyanate(FITC),creating the FITC-Fibrin-Binding Peptide(FFBP).We subsequently tested its fibrin-binding capability in vitro under static conditions,as well as under simulated flow,using real-time live confocal microscopy.Results:Fibrin stained with FFBP overlaps with fibrin stained with fibrinogen pre-labeled with Alexa Fluor 647 following coagulation induction.In contrast to pre-labeled fibrinogen,FFBP also stains already formed fibrin networks.By combining FFBP with real-time live confocal microscopy even the visualization of single fibrin fibers is possible.Conclusions:These data indicate that FFBP is a valid and valuable tool for real-time live confocal assessment of clot formation.Relevance for patients:Our findings present a valuable alternative for the visualization of fibrin even after its formation,and we believe this approach will be particularly valuable for future investigations of important,but previously overlooked,aspects of clot formation. | Nikolaj Weiss Bettina Schenk Mirjam Bachler Cristina Solomon Dietmar Fries Martin Hermann | 2017 | Journal of Clinical & Translational Research2017，3，2： | 1 |
| 9 | Algorithm-based arterial blood sampling recognition increasing safety in point-of-care diagnostics显示文摘AIM To detect blood withdrawal for patients with arterial blood pressure monitoring to increase patient safety and provide better sample dating.METHODS Blood pressure information obtained from a patient monitor was fed as a real-time data stream to an experimental medical framework. This framework was connected to an analytical application which observes changes in systolic, diastolic and mean pressure to determine anomalies in the continuous data stream. Detection was based on an increased mean blood pressure caused by the closing of the withdrawal three-way tap and an absence of systolic and diastolic measurements during this manipulation. For evaluation of the proposed algorithm, measured data from animal studies in healthy pigs were used.RESULTS Using this novel approach for processing real-time measurement data of arterial pressure monitoring, the exact time of blood withdrawal could be successfully detected retrospectively and in real-time. The algorithm was able to detect 422 of 434(97%) blood withdrawals for blood gas analysis in the retrospective analysis of 7 study trials. Additionally, 64 sampling events for other procedures like laboratory and activated clotting time analyses were detected. The proposed algorithm achieved a sensitivity of 0.97, a precision of 0.96 and an F1 score of 0.97.CONCLUSION Arterial blood pressure monitoring data can be used toperform an accurate identification of individual blood samplings in order to reduce sample mix-ups and thereby increase patient safety. | Jorg Peter Wilfried Klingert Kathrin Klingert Karolin Thiel Daniel Wulff Alfred Konigsrainer Wolfgang Rosenstiel Martin Schenk | 2017 | World Journal of Critical Care Medicine2017，6，3： | 0 |
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