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| 1 | Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization显示文摘AIM: To determine the role of interleukin (IL)-17 in gastric ulcerogenesis. METHODS: Thirty-six gastric ulcer (GU) patients and 29 non-ulcer (NU) patients were enrolled in this study. Mucosal biopsy samples were obtained from the gastric antrum and GU site during endoscopy. Samples were used in in situ stimulation for 48 h in the presence of 10 μg/mL phytohemagglutinin-P (PHA), histological examination, and Helicobacter pylori(H pylori) culture. IL-17 and IL-8 protein levels in culture supernatants were assayed by ELISA. IL-17 mRNA expression was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). H pylori cagA and vacA status was assessed by reverse hybridization using a line probe assay (LiPA). IL-8 levels in culture supernatants were assayed after AGS cells were co-cultured with H pylori strain 26 695 or recombinant human (rh) IL-17. RESULTS: All 36 GU patients and 15 of 29 NU patients were found to be H pylori-positive, while 14 NU patients were H pylori-negative. All 51 H pylori strains from both GU and NU patients were cagA- and vacAs1/m1-positive. Antral mucosal tissues from H pylori-positive patients contained significantly (H pylori-positive NU patients: median 467 pg/mg/protein, range 53-2 499; H pylori-negative NU patients: median 104 pg/mg/protein, range 16-312, P<0.0005) higher levels of IL-17 than those from uninfected patients. IL-17 levels at the ulcer site were significantly (ulcer site: median 1356 pg/mg/protein, range 121-1 3730; antrum: median 761 pg/mg/protein, range 24-7 620, P<0.005) higher than those at distant sites in the antrum. Biopsies from H pylori-positive GU and NU patients showed IL-17 mRNA expression in all samples whereas those from the antrum of the H pylori-negative controls showed no detectable expression. A significant correlation was seen between IL-17 and IL-8 levels at each biopsy site (ulcer: r= 0.62, P<0.0001; antrum: r = 0.61, P<0.0001) in GU patients. RhIL-17 and H pylori strain 26 695 each stimulated IL-8 production from AGS cells. CONCLUSION: IL-17 may play an important role in the inflammatory response to H pylori colonization, and may ultimately influence the outcome of H pylori-associated diseases that arise within the context of gastritis. | Tomokazu Mizuno Takafumi Ando Kazuo Nobata Tomoyuki Tsuzuki Osamu Maeda Osamu Watanabe Masaaki Minami Kenji Ina Kazuo Kusugami Richard M. Peek Hidemi Goto | 2005 | World Journal of Gastroenterology2005，11，40： | 18 |
| 2 | Phenotypic and functional analysis of lamina propria mononuclear cells from colono-scopic biopsy specimens in patients with ulcerative colitis显示文摘 | Haruia J Kusugami K Kuroiwa A | 1992 | Am Gastroenterology1992，87，4： | 1 |
| 3 | Increased muco sal production of granulocyte colony stimulating factor is re- lated to a delay in neutrophil apoptosis in inflammatory bow- el desease 显示文摘 | Ina K Kusugami K Hosokawa T etal | 1999 | Gastroenterol Hepatol1999，14，1： | 1 |
| 4 | Elevation ofinterleukin-6 in inflammatory bowel disease is macrophageand epithelinal cell-dependent显示文摘 | Kusugami K Fukatsu A Tanimoto M | 1995 | Dig Dis Sci1995，40，： | 1 |
| 5 | Mucosal interleukin-8 is involved in neutrophil migration and binding to extracellular matrix in inflammatory bowel disease显示文摘 | Ina K Kusugami K Yamaguchi T | 1997 | Am J Gastroenterol1997，92，8： | 1 |
| 6 | Effect of recombinant gamma interferon on natural killer cell activity in patients with gastric cancer显示文摘 | Yutaka Kuwahara M.D. Kazuo Kusugami M.D. Kimitomo Morise M.D. Kaoru Shimokata M.D | 1987 | Gastroenterologia Japonica1987，，4： | 1 |
| 7 | Clinico-pathological features of superficial depressed-type colorectalneoplastic lesions 显示文摘 | Hayakawa M Shimokawa K Kusugami K | 1999 | Am J Gastroenterol1999，94，4： | 1 |
| 8 | Clinicopathological features of superficial depressed-type colorec- tal neoplastic lesions显示文摘 | Hayakawa M Shimokawa K Kusugami K | 1999 | Am J Gastroenterol1999，94，4： | 1 |
| 9 | Clinicopathological features of superficial depressed type colorectal neoplastic lesions显示文摘 | Hayakawa M Shimokawa K Kusugami K | 1999 | Am J Gastroenterol1999，94，4： | 1 |
| 10 | Suppressive effects of cyclosporine A on neutrophils and T cells may be related to therapeutic benefits in patients with steroid-resistant ulcerative colitis 显示文摘 | Ina K Kusugami K Shimada M | 2002 | Inflamm Bowel Dis2002，8，： | 1 |
| 11 | Interleukin-8 activity correlates with histological severity in helicobacter pylori-associated antral gastritis 显示文摘 | Takafumi A Kusugami K Ohsuga M | 1996 | AM J Gastroenterol1996，91，6： | 1 |
| 12 | Troxipide, a novel antiulcer compound, has inhibitory effects on human neutrophil migration and activation induced by various stimulates显示文摘 | KUSUGAMI K INA K HOSOKAWA T | 2000 | Dig Liver Dis2000，32，4： | 1 |
| 13 | Helicobacter pylori and gut hormones显示文摘 | Hiroshi Kaneko Toshihiro Konagaya Kazuo Kusugami | 2002 | Journal of Gastroenterology2002，，2： | 1 |
| 14 | Phenotypic and functional analysis of lamina propria mononuclear cells from colonoscopic biopsy specimens in patients with ulcerative colitis显示文摘 | Haruta J Kusugami K Kuroiwa A | 1992 | Am J Gastroenterol1992，87，4： | 1 |
| 15 | Interleukin-8 activity correlates with histological severity in Helicobacter pylori-assoeiated antral gastritis 显示文摘 | Ando T Kusugami K Ohsuga M | 1996 | Am J Gastroenterol1996，91，6： | 1 |
| 16 | Suppressive effects of cyclosporine A on neutrophils and T cells may be related to therapeutic benefits in patients with steroid-resistant ulcerative colitis显示文摘 | Ina K Kusugami K Shimada M | 2002 | Inflamm Bowel Dis2002，8，1： | 1 |
| 17 | Immunomodulatory therapy for inflammatory bowel disease显示文摘 | Kusugami K Ina K Ando T | 2004 | J Gastroenterol2004，39，12： | 1 |
| 18 | Helicobacter pyloriand gut hormones显示文摘 | Kaneko H Konagaya T Kusugami K | 2002 | J Gastroenterol2002，37，2： | 1 |
| 19 | A pilot randomized trial of a human anti-interleukin-6 receptor monoclonal antibody in active Crohn’s disease显示文摘 | Hiroaki Ito Masakazu Takazoe Yoshihiro Fukuda Toshifumi Hibi Kazuo Kusugami Akira Andoh Takayuki Matsumoto Takehira Yamamura Junichi Azuma Norihiro Nishimoto Kazuyuki Yoshizaki Takashi Shimoyama Tadamitsu Kishimoto | 2004 | Gastroenterology2004，，4： | 1 |
| 20 | Disseminated intravascular coagulation associated with systemic cytomegalovirus infection during cyclosporine therapy for ulcerative colitis: report of a case显示文摘 | Hibi K Kusugami K Ina K | 2006 | Dis Colon Rectum2006，49，1： | 1 |
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