| 1 | Extent of liver resection modulates the activation of transcription factors and the production of cytokines involved in liver regeneration显示文摘AIM:To investigate the molecular events involved in liver regeneration following subtotal hepatectomy (SH) as previous studies have largely focused on partial hepatectomy (PH). METHODS: Male Wistar rats were subjected to 70% PH or 90% SH, respectively, and sacrificed at different times after surgery. Untreated and sham-operated animals served as controls. Serum and liver samples were obtained to investigate liver function, apoptosis (TUNEL assay) and transcription factors (NF-κB,Stat3; ELISA) or cytokines (HGF, TNF-a,IL-6,TGF-a,TGF-b; quantitative RT-PCR) involved in liver regeneration. RESULTS:Serum levels of ALT and AST in animals with 70% PH differed significantly from sham-operated and control animals. We found that the peak concentration 12 h after surgery returned to control levels 7 d after surgery. LDH was increased only at 12 h after 70% PH compared to sham. Bilirubin showed no differences between the sham and 70% resection. After PH, early NF-κB activation was detected 12 h after surgery (313.21±17.22 ng/mL), while there was no activation after SH (125.22 ± 44.36 ng/mL) compared to controls (111.43±32.68 ng/mL) at this time point. In SH, however, NF-κB activation was delayed until 24 h (475.56±144.29 ng/mL). Stat3 activation was similar in both groups. These findings correlated with suppressed and delayed induction of regenerative genes after SH (i.e. TNF-a 24 h postoperatively: 2375±1220 in 70% and 88±31 in 90%; IL-6 12h postoperatively:2547±441 in 70% and 173±82 in 90%). TUNEL staining revealed elevated apoptosis rates in SH (0.44% at 24 h;0.63% at 7d) compared to PH (0.27% at 24h; 0.15% at 7d). CONCLUSION: The molecular events involved in liver regeneration are significantly influenced by the extent of resection as SH leads to suppression and delay of liver regeneration compared to PH, which is associated with delayed activation of NF-κB and suppression of proregenerative cytokines. | Jan-Peter Sowa Jan Best Tamas Benko Maximillian Bockhorn Yanli Gu Eva-Maria Niehues Agnieska Bucchi Eva-Maria Benedetto-Castro Guido Gerken Ursula Rauen Jorg Friedrich Schlaak | 2008 | World Journal of Gastroenterology2008，14，46： | 1 |
| 2 | Evaluation and impact of different biomarkers for early detection of hepatocellular carcinoma显示文摘Worldwide,hepatocellular carcinoma(HCC)is a frequent complication of liver diseases and remains a major cause of cancer-related mortality.In addition,the prevalence of nonalcoholic steatohepatitis(NASH)as prerequisite of hepatocarcinogenesis,even in the absence of cirrhosis,is rising rapidly.The early detection of HCC has been crucial in improving the survival outcomes of those patients.However,in the mostly obese NASH population,diagnostic sensitivity of ultrasound-based HCC screening approaches is limited.On the other hand,biomarkers for HCC show promising potential to improve early detection,providing reproducible,investigator-independent results that can be used either alone or integrated with other biomarkers for scoring models.In the past,validation has been limited due to a lack of prospective longitudinal cohort studies.At present,large-scale retrospective phase-III-biomarker-development gives hope for the availability of biomarker-based screening approaches in the near future.This review focuses on the potential impact of biomarkers on surveillance strategies,potentially allowing for earlier HCC diagnosis. | Jan Best Svenja Sydor Lars P.Bechmann Ali Canbay | 2020 | Hepatoma Research2020，6，9： | 0 |
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