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| 1 | A targeted approach significantly increases the identification rate of patients with undiagnosed haemochromatosis显示文摘 | E.Cadet D.Capron A. S.Perez S. N.Crépin S.Arlot J.‐P.Ducroix M.Dautréaux P.Fardellone P.Leflon A. T.Merryweather‐Clarke K. J.Livesey J. J.Pointon P.Rose J.Harcourt J.Emery J. M.Sueur R.Feyt K. J. H.Robson J.Rochette | 2003 | Journal of Internal Medicine2003，，2： | 1 |
| 2 | AB024.Photo-oxidation of N-retinylidene-N-retinylethanolamine in vitro by high-energy visible light显示文摘Background:Age-related macular degeneration(AMD)is the second Canadian cause in visual deficiency.AMD is characterized by the death of photoreceptors and retinal pigmented epithelium(RPE)in the macular region of the retina,leading to the loss of central vision.Epidemiologic studies suggest an association between lifetime sun exposure and the probability to develop AMD even though mechanisms are unknown.Sunlight is made of about 30%of high-energy visible(HEV)light(blue light),the most energetic wavelength reaching the retina.These wavelengths can be absorbed by lipofuscin,an age pigment accumulating in RPE cells.Lipofuscin principal component is N-retinylidene-N-retinylethanolamine(A2E).Many research teams showed that absorption of HEV light by A2E in RPE cells at non-physiological doses produces free radicals and leads to cell death.Our earlier work shows that when A2E-loaded RPE cells are irradiated with HEV light at physiological doses,the same light does not lead to oxidative stress as measured by telomere and mitochondrial integrity.Our hypothesis is that HEV light,at physiological doses,modify or convert A2E in derived produces,inhibiting its photo-oxidant effect.Methods:In vitro,we irradiated A2E with HEV light with or without antioxidants and with varying irradiation regimen to observe the UV-Visible spectrum of A2E.In cellulo,we loaded ARPE-19 cells with A2E and irradiated cells at physiological levels for 4 consecutive days.We then observed A2E fluorescence using a fluorescence microscope with nucleus counterstaining with DRAQ5.Results:HEV light leads to the disappearance of A2E characteristic UV-Visible spectrum and the apparition of a new product suggesting that HEV light modifies A2E.Nor oxidation and irradiation regimen seem to have an impact in A2E’s conversion by HEV light.We observed and progressive diminution of A2E fluorescence in cellulo during physiological irradiations.Conclusions:The loss of A2E photo-oxidation capacities by HEV light seems to be caused by its conversion by HEV light.We suggest that HEV light,at physiological doses,may be protective rather than photo-toxic.The next step would be to identify A2E’derived produces and their cell toxicity. | Marie-Christine Lambert Mathieu Ouellette Élodie Boisselier Patrick J.Rochette | 2018 | Annals of Eye Science2018，，1： | 0 |
| 3 | AB044.Phototoxic stress induced in retinal pigmented epithelium cells by the synergy between polycyclic aromatic hydrocarbons and blue light显示文摘Background:Lesion to the retinal pigment epithelium(RPE)is a crucial event in age-related macular degeneration(AMD)development.Although the pathogenesis of this complex disease is poorly understood,sunlight exposure and smoking are major environmental risk factors associated with AMD.High-energy visible blue light(HEV;400-500 nm)is the most energetic and potentially harmful solar wavelengths reaching adults retina.On the other hand,RPE cells can be exposed to a large range of pollutants from cigarette smoke,with polycyclic aromatic hydrocarbons(PAH)being among the most toxic.Some PAH from cigarette smoke can absorb HEV light.This led us hypothesize that in RPE cells,the combination of PAH and HEV could synergize to exacerbate the stress caused by either factor alone.We thus investigate the combined effect of PAH and HEV light in RPE cells.Methods:Confluent RPE immortalized cells(ARPE19)were exposed to nanomolar concentrations of benzo[a]pyrene(BaP)or indeno[1,2,3-cd]pyrene(IcdP).While IcdP efficiently absorbs HEV wavelengths,BaP,the most studied PAH,does not significantly absorb HEV light and was used as a control.BaP or IcdP contaminated ARPE19 were then irradiated with increasing sub-lethal doses of HEV light(150-500 J/cm2)using a setup that mimics the light spectrum normally reaching the retina.Cytotoxicity,apoptosis and reactive oxygen species(ROS)generation were assessed in each condition.Results:In presence of low concentrations of IcdP,sub-lethal amounts of HEV light trigger,in a dose-dependent way,up to 70%of apoptotic cell death.Co-exposure to IcdP and HEV also leads to a synergistic ROS generation in ARPE19 cells,thus inducing oxidative stress.None of these effects were observed with BaP.Efficient inhibition of ROS production by specific antioxidants only decreases death by 20%in cells simultaneously exposed to both IcdP and HEV light.Conclusions:Low concentrations of IcdP synergize with HEV light to induce phototoxicity in ARPE19 cells.An increased oxidative stress results from the interaction between both agents and partially explains the enhanced HEV phototoxicity in IcdP contaminated ARPE19 cells.This suggests that another major mechanism is involved in the synergetic toxicity.For smokers,this synergy between HEV and PAH may accelerate RPE cells loss and contribute to their greater risk of developing AMD. | Corinne Zinflou Patrick J.Rochette | 2018 | Annals of Eye Science2018，，1： | 0 |
| 4 | AB047.Quebec database of clinical data and biological material for research on uveal melanoma显示文摘Background:Sharing biological material and clinical data from patients with uveal melanoma.Methods:Uveal melanoma is the most common intraocular malignancy in the adult population.Because uveal melanoma is primarily a sporadic cancer and familial cases are rare,it is difficult to prevent or detect it.Despite effective treatment of ocular tumors,more than 50%of patients develop incurable liver metastases mainly in the 5-10 years following the detection of the primary tumor.This cancer is relatively rare and the obtained biopsies are very small.About 20 samples are taken each year in Quebec.This provincial infrastructure is made of biological material from donors with uveal melanoma and a large clinical database.Collected tumor biopsies are used for culturing cell lines and the creation of a DNA/RNA library used for genomic and genetic studies.Results:This infrastructure plays an important role in the achievement of various research programs for a better understanding of genetic and environmental factors involved in the development of melanoma and the spread of metastasis.It allows collaboration with other researchers at a provincial,national and international level in order to make progress in basic and clinical research on uveal melanoma.Conclusions:The biological material and clinical data of this infrastructure are available upon request to VHRN members whose research project was approved by the ethics committee of the institution. | Solange Landreville Frédéric Mouriaux Patrick J.Rochette Christian Salesse Mohib W.Morcos Claudine Bellerive Sylvain Guérin | 2018 | Annals of Eye Science2018，，1： | 0 |
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