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| 1 | Implementing Nutrition Diagnosis, Step Two in the Nutrition Care Process and Model: Challenges and Les- sons Learned in Two Health Care Facilities 显示文摘 | Jennifer Mathieu Mandy Foust RD Patricia Ouellette RD | 2005 | Journal of the American Dietetic Association2005，105，10： | 1 |
| 2 | AB024.Photo-oxidation of N-retinylidene-N-retinylethanolamine in vitro by high-energy visible light显示文摘Background:Age-related macular degeneration(AMD)is the second Canadian cause in visual deficiency.AMD is characterized by the death of photoreceptors and retinal pigmented epithelium(RPE)in the macular region of the retina,leading to the loss of central vision.Epidemiologic studies suggest an association between lifetime sun exposure and the probability to develop AMD even though mechanisms are unknown.Sunlight is made of about 30%of high-energy visible(HEV)light(blue light),the most energetic wavelength reaching the retina.These wavelengths can be absorbed by lipofuscin,an age pigment accumulating in RPE cells.Lipofuscin principal component is N-retinylidene-N-retinylethanolamine(A2E).Many research teams showed that absorption of HEV light by A2E in RPE cells at non-physiological doses produces free radicals and leads to cell death.Our earlier work shows that when A2E-loaded RPE cells are irradiated with HEV light at physiological doses,the same light does not lead to oxidative stress as measured by telomere and mitochondrial integrity.Our hypothesis is that HEV light,at physiological doses,modify or convert A2E in derived produces,inhibiting its photo-oxidant effect.Methods:In vitro,we irradiated A2E with HEV light with or without antioxidants and with varying irradiation regimen to observe the UV-Visible spectrum of A2E.In cellulo,we loaded ARPE-19 cells with A2E and irradiated cells at physiological levels for 4 consecutive days.We then observed A2E fluorescence using a fluorescence microscope with nucleus counterstaining with DRAQ5.Results:HEV light leads to the disappearance of A2E characteristic UV-Visible spectrum and the apparition of a new product suggesting that HEV light modifies A2E.Nor oxidation and irradiation regimen seem to have an impact in A2E’s conversion by HEV light.We observed and progressive diminution of A2E fluorescence in cellulo during physiological irradiations.Conclusions:The loss of A2E photo-oxidation capacities by HEV light seems to be caused by its conversion by HEV light.We suggest that HEV light,at physiological doses,may be protective rather than photo-toxic.The next step would be to identify A2E’derived produces and their cell toxicity. | Marie-Christine Lambert Mathieu Ouellette Élodie Boisselier Patrick J.Rochette | 2018 | Annals of Eye Science2018，，1： | 0 |
| 3 | AB082.Gold nanoparticles as a new drug vector for glaucoma therapy显示文摘Background:Glaucoma is an optical neuropathy affecting over 67 million people in the world.Efficiency of current active molecules,as travoprost(hydrophobic)is limited when administered by ophthalmic drops.Indeed,more than 99.9%is discarded due to multiple factors including lacrimal drainage.Low retention time of drugs at the cornea leads to their poor penetration.The aim of the project is to develop a drug delivery system allowing the drug penetration through biological barriers.Our hypothesis is that a drug delivery system based on gold nanoparticles should enhance the efficiency of the drugs.The main objective is to study the encapsulation ability of gold nanoparticles towards travoprost.The specific objectives are(I)the synthesis and characterizations of gold nanoparticles;(II)the establishment of the encapsulation protocol;(III)the method development of the separation of free and encapsulated drugs and;(IV)the quantification of the encapsulated drugs.Methods:Gold nanoparticles were synthesized by a new method developed in our laboratory.An encapsulation protocol was settled using aqueous conditions at 37℃.The separation of free and encapsulated drugs was performed with magnetic beads.The quantification of the encapsulated drugs was then performed by high performance liquid chromatography and confirmed by UV-visible spectroscopy.Results:Gold nanoparticles of 28±1 nm were synthesized and purified according to our new experimental conditions.The encapsulation protocol lasts 5 days in the optimised conditions.The separation method involving magnetic beads was optimized to get rid of non-specific interactions.The travoprost was incubated with the nanoparticles until the reach of equilibrium in solution.Conclusions:We showed that active molecules used for glaucoma therapy,as travoprost,can be encapsulated in gold nanoparticles.Further analysis will allow identifying the encapsulation properties of various gold nanoparticles,differing by their size,shape and chemical surface.These data suggest the possible improvements. | Florence Masse Mathieu Ouellette Elodie Boisselier | 2018 | Annals of Eye Science2018，，1： | 0 |
| 4 | AB023.Ultrastable gold nanoparticles as a drug delivery system for ocular therapy显示文摘Background:Ocular therapy administrated by ophthalmic drops is advantageous thanks to its simplicity.However,efficiency of active molecules is limited when administered by this method.Indeed,more than 99.9%is discarded due to multiple factors including lacrimal drainage.Low retention time of drugs at the cornea leads to their poor penetration.Our hypothesis is that a drug delivery system based on gold nanoparticles should enhance the efficiency of the drugs.The main objective is to develop new methods to improve active molecules biodisponibility in ocular therapy thanks to a new drug delivery system implying gold nanoparticles.The specific objectives are:(I)to synthesize and characterize ultrastable gold nanoparticles,(II)to establish the drug encapsulation protocol,(III)to develop a separation method of free and encapsulated drugs to allow their quantification,(IV)to study the cytotoxicity of our gold nanoparticles.Methods:Ultrastable gold nanoparticles were synthesized by a new method and their ultrastability toward several harsh conditions was characterized.An encapsulation protocol was settled for several drugs.The separation of free and encapsulated drugs was performed with magnetic beads.The quantification of the encapsulated drugs was performed by HPLC.A MTS assay was performed on 3 corneal epithelial cell populations,exposed or unexposed to gold nanoparticles.Reconstructed corneas were prepared using the self-assembly method.A wound healing experience was performed on those corneas with or without nanoparticles.Results:Gold nanoparticles were synthesized and purified according to our new experimental conditions.They support harsh conditions as several cycles of freeze-drying,heating,salt exposition and ultracentrifugation.For the first time in literature,gold nanoparticle support autoclave sterilisation.The separation method involving magnetic beads was optimized to get rid of non-specific interactions.The encapsulation efficiency varies according to the active molecule.The MTS assay did not show diminution of the cellular viability when in presence of gold nanoparticles.Furthermore,gold nanoparticle exposition did not slow the wound healing of reconstructed corneas.Conclusions:Our new ultrastable gold nanoparticles can have a major impact in nanomedicine.They can support harsh conditions,as autoclave treatment,allowing their sterilisation for in vivo use.We showed that active molecules can be encapsulated in gold nanoparticles.In addition,they do not seem to cause any diminution of cellular viability.These data suggest the possible improvements in ocular therapy thanks to gold nanoparticles. | Florence Masse Pascale Desjardins Mathieu Ouellette Camille Couture Mahmoud Mohamed Omar Vincent Pernet Sylvain Guérin Élodie Boisselier | 2019 | Annals of Eye Science2019，，1： | 0 |
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