| 1 | Isolation and Preliminary Identification of Broad-spectrum Bacteriocin-producing Lactobacillus Strain CW3显示文摘[Objective]This study aimed to screen and preliminarily identify Lactobacillus strain CW3. [Method]Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriocin-producing Lactobacillus strain; after eliminating the interference of organic acid and hydrogen peroxide,antibacterial substances containing protein properties produced by the screened strain were detected; finally,the obtained strain was identified. [Result]After elimination of interference factors such as organic acid and hydrogen peroxide,the fermentation supernatant of strain CW3 could still inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase,which indicated that the produced antibacterial substances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [Conclusion]CW3is a broad-spectrum bacteriocin-producing Lactobacillus plantarum strain. | Haoxin LV Weidong WANG Zhongfang TAN Feifei YANG Yingchun JIAO Yanping WANG Zongwei LI | 2014 | Agricultural Biotechnology2014，3，1： | 2 |
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