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| 1 | One-year clinical study of NeuroR egen scaffold implantation following scar resection in complete chronic spinal cord injury patients显示文摘The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5–4.5 cm were surgically resected in five complete chronic spinal cord injury(SCI) patients. The NeuroR egen scaffold along with autologous bone marrow mononuclear cells(BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or Neuro Regen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials(SSEP) from the lower limbs was also detected. The results indicate that scar resection and Neuro Regen scaffold transplantation could be a promising clinical approach to treating SCI. | Zhifeng Xiao Fengwu Tang Jiaguang Tang Huilin Yang Yannan Zhao Bing Chen Sufang Han Nuo Wang Xing Li Shixiang Cheng Guang Han Changyu Zhao Xiaoxiong Yang Yumei Chen Qin Shi Shuxun Hou Sai Zhang Jianwu Dai | 2016 | Science China(Life Sciences)2016，59，7： | 18 |
| 2 | Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth显示文摘The inhibitory effect of three berberine alkaloids (BAs) from Coptis chinensis Franch, a traditional Chinese medicinal (TCM) herb, on Bifidobacterium adolescentis growth was investigated by micro- calorimetry. The power-time curves of B. adolescentis with and without BAs were acquired, meanwhile the extent and duration of inhibitory effect on the metabolism were evaluated by the growth rate con- stant (k), half inhibitory ratio (IC50), maximum heat-output power (Pmax), peak time of maximum heat-output power (tp) and total heat production (Qt). k, Pmax and Qt decreased, and tp was prolonged with the increase of BAs concentration. The IC50 of BAs is 806 μg/mL for berberine, 341 μg/mL for cop- tisine and 236 μg/mL for palmatine. The sequence of antimicrobial activity of BAs is berberine < cop- tisine < palmatine. Combined with previous studies, it could be shown that the sequences of antim- icrobial activity of BAs on both Bacillus shigae and Escherichia coli are berberine > coptisine > pal- matine. The structure-function relationship of BAs indicates that the functional group methylenedioxy or methoxyl at C2 and C3 might be the major group inducing the activities of BAs on E. coli and B. adolescentis. Meanwhile, the substituent groups at C2, C3, C9 and C10 almost have equal effect on B. shigae. | YAN Dan WEI Li XIAO XiaoHe ZHOU DanLei HAN YuMei | 2009 | Chinese Science Bulletin2009，54，3： | 12 |
| 3 | Genome-wide Analysis of MYB Gene Family in Potato Provides Insights into Tissue-specific Regulation of Anthocyanin Biosynthesis显示文摘The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In this study,A total of 217 MYB genes,including 901R-MYBs,124 R2R3-MYBs,and 3 R1R2R3-MYBs have been identified from the potato genome.The 1R-MYB and R2R3-MYB family members could be divided into 20 and 35 subgroups respectively.Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization,further supporting the results of phylogenetic analysis.Potato is an ideal plant to reveal the tissue-specific anthocyanins biosynthesis regulated by MYB,as the anthocyanins could be accumulated in different tissues,showing colorful phenotypes.Five pairs of colored and colorless tissues,stigma,petal,stem,leaf,and tuber flesh,were applied to the transcriptomic analysis.A total of 70 MYB genes were found to be differentially expressed between colored and colorless tissues,and these differentially expressed genes were suspected to regulate the biosynthesis of anthocyanin of different tissues.Co-expression analysis identified numerous potential interactive regulators of anthocyanins biosynthesis,involving 39 MYBs,24 bHLHs,2 WD-repeats,and 29 biosynthesis genes.Genome-wide association study(GWAS)of tuber flesh color revealed amajor signal at the end of Chromosome 10,which was co-localized with reported I gene(StMYB88),controlling tuber peel color.Analyses of DEGs(Differentially Expression Genes)revealed that both StMYB88 and StMYB89 were closely related to regulating anthocyanin biosynthesis of tuber flesh.This work offers a comprehensive overview of the MYB family in potato and will lay a foundation for the functional validation of these genes in the tissue-specific regulation of anthocyanin biosynthesis. | Yumei Li Jun Liang Xiangzhi Zeng Han Guo Youwei Luo Philip Kear Shouming Zhang Guangtao Zhu | 2021 | Horticultural Plant Journal2021，7，2： | 8 |
| 4 | Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L显示文摘Clubroot disease,a major plant root disease caused by Plasmodiophora brassicae,has become one of the most destructive diseases among cultivated cruciferous vegetables.However,clubroot-resistant Brassica oleracea materials are rare.A few clubroot-resistant cabbage varieties are available on the market,but all are Ogura cytoplasmic male sterile(CMS)types.Therefore,in this study,to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage,a new fertility-restored Ogura CMS material,16Q2-11,was used as a bridge to transfer the clubroot resistance(CR)gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method(a fertility restoration and cytoplasm replacement method).In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage(FRCRC),16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene CRb2.Eleven Rfo-positive progenies were generated,of which four contained CRb2:F8-514,F8-620,F8-732 and F8-839.After inoculation with race 4 of P.brassicae,these four CRb2-positive individuals showed resistance.Furthermore,F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines,resulting in the successful introgression of the CRb2 gene into the inbred lines.All offspring produced from this step of cross,which had a normal cytoplasm,showed a high resistance to race 4 of P.brassicae and could be utilized for the breeding of clubrootresistant cabbage varieties in the future.This is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages,which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in Brassica crops. | Wenjing Ren Zhiyuan Li Fengqing Han Bin Zhang Xing Li Zhiyuan Fang Limei Yang Mu Zhuang Honghao Lv Yumei Liu Yong Wang Hailong Yu Yangyong Zhang | 2020 | Horticulture Research2020，7，1： | 7 |
| 5 | The action of medicinal animal horns on Escherichia coli growth investigated by microcalorimetry and chemometric analysis显示文摘The action of Cornu Cervi Pantotrichum (CCP), Cornu Cervi (CC) and Cornu Saigae Tataricae (CST) on Escherichia coli growth were investigated using microcalorimetry to find the heat change regularity of microbial growth. The similarity of thermogenic curves and thermodynamics parameters were investigated as evaluation index, such as the growth rate constant in the first expo- nential phase (k1), maximum power in the first exponential phase (P1), maximum power in the secondary exponential phase (P2), peak time in the first exponential phase (T1), peak time in the stationary phase (T2) and the total heat production in stage 1 (Q1), and the total heat production in stage 2 (Q2). Chemometric analysis was used as a reference for the bioactivity evaluation of medicinal animal horns. The results indicated that the similarity between CST and the control was smaller than that between CCP, CC and the control. Both CCP and CC could increase the heat in the microbial growth, whereas CST decreased it. The biotic thermal activity of different medicinal animal horns was objectively, qualitatively, and quantitatively evaluated by the similarity of thermogenic curves and thermodynamics parameters analysis. | YAN Dan HAN YuMei LUO JiaoYang ZHANG Ping TANG HuiYing PENG Cheng XIAO XiaoHe | 2010 | Chinese Science Bulletin2010，55，26： | 6 |
| 6 | Remineralization of enamel caries by an amelogenin-derived peptide and fluoride in vitro显示文摘Dental caries is one of the most common oral diseases in the world.This study was tantamount to investigate the combinatory effects of an amelogenin-derived peptide(called QP5)and fluoride on the remineralization of artificial enamel caries.The peptide QP5 was synthesized and characterized,and the binding capability of the peptide on hydroxyapatite(HA)and demineralized tooth enamel surface was analysed.Then,the mineralization function of the peptide and fluoride was studied through the spontaneous mineralization testing and remineralization on enamel caries in vitro.First,the novel peptide QP5 could bind on the hydroxyapatite and demineralized tooth enamel surfaces.Second,QP5 can transitorily stabilize the formation of amorphous calcium phosphate and direct the transformation into hydroxyapatite crystals alone and in combination with fluoride.In addition,compared to blocks treated by peptide QP5 alone or fluoride,the sample blocks showed significantly higher surface microhardness,lower mineral loss and shallower lesion depth after treatment with a combination of QP5 and fluoride at high or low concentrations.The peptide QP5 could control the crystallization of hydroxyapatite,and combinatory application of peptide QP5 and fluoride had a potential synergistic effect on the remineralization of enamel caries. | Longjiang Ding Sili Han Kun Wang Sainan Zheng Wenyue Zheng Xiu Peng Yumei Niu Wei Li Linglin Zhang | 2020 | Regenerative Biomaterials2020，7，3： | 6 |
| 7 | Molecular recognition study on supramolecular system (Ⅶ)-——Chiral recognition of amino acids with α-cyclodextrin using competitive inclusion method显示文摘CYCLODEXTRINS (CDs) have been extensively studied. The studies mainly focused on their in-clusion complexation with guest molecules for mimicking the enzyme-substrate specific interac-tion. The molecular recognition mainly involves several classes of organic compounds, such ashydrocarbon, aliphatic alcohols, aliphatic diols, phenols, cyclohexane derivatives, naphtha-lene derivatives, and other aromatic compounds, but few amino acid molecules. | LIU Yu, QI Aidi, HAN Baohang, LI Yumei, ZHANG Yimin and CHEN RongtiDepartment of Chemistry, Nankai University, Tianjin 300071, ChinaOn the leave from Tianjin College of Traditional Chinese Medicine,tianjin 300193,China | 1997 | Chinese Science Bulletin1997，42，14： | 4 |
| 8 | Effect of Wheel Load on Wheel Vibration and Sound Radiation显示文摘The current researches of wheel vibration and sound radiation mainly focus on the low noise damped wheel. Compared with the traditional research, the relationship between the sound and wheel/rail contact is difficulty and worth studying. However, there are few studies on the effect of wheel load on wheel vibration and sound radiation. In this paper, laboratory test carried out in a semi-anechoic room investigates the effect of wheel load on wheel natural frequencies, damping ratios, wheel vibration and its sound radiation. The laboratory test results show that the vibration of the wheel and total sound radiation decrease significantly with the increase of the wheel load from 0 t to 1 t. The sound energy level of the wheel decreases by 3.7 d B. When the wheel load exceeds 1 t, the attenuation trend of the vibration and sound radiation of the wheel becomes slow. And the increase of the wheel load causes the growth of the wheel natural frequencies and the mode damping ratios. Based on the finite element method(FEM) and boundary element method(BEM), a rolling noise prediction model is developed to calculate the influence of wheel load on the wheel vibration and sound radiation. In the calculation, the used wheel/rail excitation is the measured wheel/rail roughness. The calculated results show that the sound power level of the wheel decreases by about 0.4 dB when the wheel load increases by 0.5 t. The sound radiation of the wheel decreases slowly with wheel load increase, and this conclusion is verified by the field test. This research systematically studies the effect of wheel load on wheel vibration and sound radiation, gives the relationship between the sound and wheel/rail contact and analyzes the reasons, therefore, it provides a reference for further research. | HAN Jian WANG Ruiqian WANG Di GUAN Qinghua ZHANG Yumei XIAO Xinbiao JIN Xuesong | 2015 | Chinese Journal of Mechanical Engineering2015，28，1： | 3 |
| 9 | Long-term clinical observation of patients with acute and chronic complete spinal cord injury after transplantation of Neuro Regen scaffold显示文摘Spinal cord injury(SCI)often results in an inhibitory environment at the injury site.In our previous studies,transplantation of a scaffold combined with stem cells was proven to induce neural regeneration in animal models of complete SCI.Based on these preclinical studies,collagen scaffolds loaded with the patients’own bone marrow mononuclear cells or human umbilical cord mesenchymal stem cells were transplanted into SCI patients.Fifteen patients with acute complete SCI and 51 patients with chronic complete SCI were enrolled and followed up for 2 to 5 years.No serious adverse events related to functional scaffold transplantation were observed.Among the patients with acute SCI,five patients achieved expansion of their sensory positions and six patients recovered sensation in the bowel or bladder.Additionally,four patients regained voluntary walking ability accompanied by reconnection of neural signal transduction.Among patients with chronic SCI,16 patients achieved expansion of their sensation level and 30 patients experienced enhanced reflexive defecation sensation or increased skin sweating below the injury site.Nearly half of the patients with chronic cervical SCI developed enhanced finger activity.These long-term follow-up results suggest that functional scaffold transplantation may represent a feasible treatment for patients with complete SCI. | Fengwu Tang Jiaguang Tang Yannan Zhao Jiaojiao Zhang Zhifeng Xiao Bing Chen Guang Han Na Yin Xianfeng Jiang Changyu Zhao Shixiang Cheng Ziqiang Wang Yumei Chen Qiaoling Chen Keran Song Zhiwei Zhang Junjie Niu Lingjun Wang Qin Shi Liang Chen Huilin Yang Shuxun Hou Sai Zhang Jianwu Dai | 2022 | Science China(Life Sciences)2022，65，5： | 3 |
| 10 | UV-enhanced bioactivity and cell response of micro-arc oxidized titania coatings显示文摘 | Yong Han Donghui Chen Jifeng Sun Yumei Zhang Kewei Xu | 2008 | Acta Biomaterialia2008，，5： | 2 |
| 11 | Synthesis, photostability and bioactivity of 2,3-cyclopropanated abscisic acid显示文摘 | Liu Wenjian Han Xiaoqiang Xiao Yumei Fan Jinlong Zhang Yuanzhi Lu Huizhe Wang Mingan Qin Zhaohai | 2013 | Phytochemistry2013，，： | 1 |
| 12 | Metarhizium taii var. chongqingensis Nov., Anamorph of Cordyceps chongqingensis sp. Nov. Isolated from a Low Altitude Area in Chongqing, China显示文摘 | Yanhong Yang Shaoxi Cai Yimin Zheng Xiumin Lu Xiuying Xu Yumei Han | 2009 | Current Microbiology2009，，6： | 1 |
| 13 | Antithrombotic Management of Atrial Fibrillation Patients Presenting with Coronary Artery Disease:A Consensus Document of the Chinese Society of Cardiology显示文摘The coexistence of coronary artery disease(CAD)and atrial fibrillation(AF)is common in clinical practice.Patients with CAD require antiplatelet therapy to reduce the occurrence of myocardial ischemic events.However,patients with AF at high risk of thromboembolism require oral anticoagulants to reduce the occurrence of thromboembolic events such as stroke.In cases where CAD coexists with AF,the combined use of antiplatelet and anticoagulation therapy can effectively reduce the risk of ischemic and thromboembolic events but increase the risk of bleeding at the same time.The optimal antithrombotic regimen in patients with both CAD and AF has been controversial in clinical practice.In recent years,the expert consensuses on antithrombotic therapy in patients with AF presenting with acute coronary syndrome and/or undergoing percutaneous coronary intervention have been successively released in Europe and North America,and have been updated in a timely manner.In contrast,the guidelines on antithrombotic therapies in China are lacking.Based on published clinical evidence,this consensus incorporated relevant international and Chinese guidelines,consensuses,and expert recommendations,and addressed the issues encountered in the clinical practice of antithrombotic therapy in patients with AF and different types of CAD.The current guideline is of great significance to guide treatment in patients with both CAD and AF in China. | Chinese Society of Cardiology of Chinese Medical Association Editorial Board of Chinese Journal of Cardiology Zuyi Yuan Shulin Wu Yaling Han Peng Qu Shaowen Liu Changsheng Ma Yumei Xue Xinjun Lei | 2021 | Cardiology Discovery2021，1，1： | 1 |
| 14 | Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis显示文摘 | Yongquan Shi Huihong Zhai Xin Wang Zheyi Han Changjiang Liu Mei Lan Jingping Du Changcun Guo Yumei Zhang Kaichun Wu Daiming Fan | 2004 | Experimental Cell Research2004，，2： | 1 |
| 15 | Activation of the receptor for advanced glycation end products (RAGE) exacerbates experimental autoimmune myasthenia gravis symptoms显示文摘 | Lili Mu Yao Zhang Bo Sun Jinghua Wang Xiaoli Xie Na Li Jia Zhang Qingfei Kong Yumei Liu Zhijuan Han Guangyou Wang Zheng Fu Bo Yu Guozhong Li Hulun Li | 2011 | Clinical Immunology2011，，1： | 1 |
| 16 | High-Throughput Multiplex Sequencing to Discover Copy Number Variants in Drosophila显示文摘 | Daines Bryce Wang Hui Li Yumei Han Yi Gibbs Richard Chen Rui | 2009 | Genetics2009，，4： | 1 |
| 17 | Effect of climate change over the past half century on the distribution, extent and NPP of ecosystems of Inner Mongolia 显示文摘 | ZHANG Guoguang KANG Yumei HAN Guodong | 2011 | Global Change Biology2011，17，1： | 1 |
| 18 | A genome wide association study between copy number variation (CNV) and human height in Chinese population显示文摘Copy number variation (CNV) is a type of genetic variation which may have important roles in phenotypic variability and disease susceptibility. To hunt for genetic variants underlying human height variation, we performed a genome wide CNV association study for human height in 618 Chinese unrelated subjects using Affymetrix 500K array set. After adjusting for age and sex, we found that four CNVs at 6p21.3, 8p23.3-23.2, 9p23 and 16p12.1 were associated with human height (with borderline significant p value: 0.013, 0.011, 0.024, 0.049; respectively). However, after multiple tests correction, none of them was associated with human height. We observed that the gain of copy number (more than 2 copies) at 8p23.3-23.2 was associated with lower height (normal copy number vs. gain of copy number: 161.2 cm vs. 153.7 cm, p = 0.011), which accounted for 0.9% of height variation. Loss of copy number (less than 2 copies) at 6p21.3 was associated with 0.8% lower height (loss of copy number vs. normal copy number: 154.5 cm vs. 161.1 cm, p = 0.013). Since no important genes influencing height located in CNVs at loci of 8p23.3-23.2 and 6p21.3, the two CNVs may cause the structural rear- rangements of neighbored important candidate genes, thus regulates the variation of height. Our results expand our knowledge of the genetic factors underlying height variation and the biological regulation of human height. | Xi Li Lijun Tan Xiaogang Liu Shufeng Lei Tielin Yang Xiangding Chen Fang Zhang Yue Fang Yan Guo Liang Zhang Han Yan Feng Pan Zhixin Zhang Yumei Peng Qi Zhou Lina He Xuezhen Zhu Jing Cheng Lishu Zhang Yaozhong Liu Qing Tian Hongwen Deng | 2010 | Journal of Genetics and Genomics2010，37，12： | 1 |
| 19 | Bio-characteristic profiling related to clinic:A new technology platform for quality evaluation of Chinese materia medica显示文摘中药质量评价是制约中药现代化和国际化的瓶颈问题。由于中药自身多组分、多功效、多靶点的特殊性,现有中药质量评价体系仍难以充分满足中药质量控制需求。为此,笔者提出关联临床有效性和安全性的“临床生物特征谱学”学术概念,借助具有临床监测优势或谱学特征的分析方法,构建“临床生物特征谱学”新技术平台,以期实现“实时、动态、全程监控”的中药质量评价新技术体系,有助于提升其临床安全性和有效性,推动中药产业持续健康发展。 | Li-Na Wen Ai-Ting Wang Na Guo Yumei Han Can Yan Dan Yan | 2019 | TMR Modern Herbal Medicine2019，2，4： | 1 |
| 20 | A broadly neutralizing antibody against SARS-CoV-2 Omicron variant infection exhibiting a novel trimer dimer conformation in spike protein binding显示文摘Dear Editor,The constant evolution of SARS-CoV-2 has resulted in the emergence of circulating variants during the COVID-19 pandemic.Recently,a newly emerged B.1.1.529(Omicron)variant raised global concern.Compared with the original SARS-CoV-2 strain and other variants of concern(VOCs),the Omicron variant has more than 30 mutations on its spike(S)protein,of which 16 mutations are located in the receptor-binding domain(RBD). | Yingdan Wang Wuqiang Zhan Jiangyan Liu Yanqun Wang Xiang Zhang Meng Zhang Lin Han Yunping Ma Lu Lu Yumei Wen Zhenguo Chen Jincun Zhao Fan Wu Lei Sun Jinghe Huang | 2022 | Cell Research2022，32，9： | 1 |
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