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| 1 | Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9显示文摘A narrow leaf mutant was isolated from transgenic rice(Oryza sativa L.)lines carrying a T-DNA insertion.The mutant is characterized by narrow leaves during its whole growth period,and was named nal9(narrow leaf 9).The mutant also has other phenotypes,such as light green leaves at the seedling stage,reduced plant height,a small panicle and increased tillering.Genetic analysis revealed that the mutation is controlled by a single recessive gene.A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion,so a map-based cloning strategy was employed to isolate the nal9 gene.The mutant individuals from the F2generations of a cross between the nal9 mutant and Longtepu were used for mapping.With 24 F2mutants,the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3.New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156.The nal9 gene was fnally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking.Sequence and expression analysis showed that an ATP-dependent Clp protease proteolytic subunit gene(ClpP)was most likely to be the nal9 gene.Furthermore,the nal9 mutation was rescued by transformation of the ClpP cDNA driven by the 35S promoter.Accordingly,the ClpP gene was identifed as the NAL9 gene.Our results provide a basis for functional studies of NAL9 in future work. | Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu | 2013 | Journal of Integrative Plant Biology2013，55，11： | 8 |
| 2 | A comparative study on different regeneration processes of Pt-Sn/γ-Al2O3 catalysts for propane dehydrogenation显示文摘Three different regeneration processes including hydrogen or nitrogen purging and coke-burning treatment were used to restore the Pt-Sn/γ-Al_2O_3 catalysts, through which propane dehydrogenation reaction was performed in a consecutive reaction-regeneration mode. It was found that the catalyst using hydrogen regeneration showed the best stability compared with those regenerated by nitrogen purging and coke-burning treatment, suggesting that hydrogen regeneration is an effective approach for maintaining the performance of Pt-Sn/γ-Al_2O_3 catalysts in propane dehydrogenation reaction. The effect of different regeneration atmospheres on the metal active center and the coke deposition was investigated by XRD,TEM, N_2-physisorption, TPO, TG and Raman technologies, and the results revealed that hydrogen or nitrogen regeneration resulted in little impact on the size and structure of metal active center, retaining the effective Pt Sn phase over the catalyst. Moreover, hydrogen regeneration not only removed the low dense components of the coke, but also altered the property of the residual coke through hydrogenation, leading to a higher mobility of coke, and thus a higher accessibility of the metal active centers. Whereas nitrogen regeneration only removed the low dense components of the coke. Although coke-burning regeneration caused a thorough coke removal, the catalyst subjected to repeated redox exhibited poor stability due to metal agglomeration, phase segregation and the resulting large Pt_3Sn particle and core-shell structure with a Sn-rich surface. | Changyong Sun Junyin Luo Mingjin Cao Ping Zheng Guocheng Li Jiahao Bu Zhou Cao Shihong Chen Xiaowei Xie | 2018 | Journal of Energy Chemistry2018，27，1： | 5 |
| 3 | The role of China's terrestrial carbon sequestration 2010-2060 in offsetting energy-related CO_(2) emissions显示文摘Energy consumption dominates annual CO_(2) emissions in China.It is essential to significantly reduce CO_(2) emissions from energy consumption to reach national carbon neutrality by 2060,while the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions cannot be underestimated.Natural climate solutions(NCS),including improvements in terrestrial carbon sequestration,represent readily deployable options to offset anthropogenic greenhouse gas emissions.However,the extent to which China’s terrestrial carbon sequestration in the future,especially when target-oriented managements(TOMs)are implemented,can help to mitigate energy-related CO_(2) emissions is far from certain.By synthesizing available findings and using several parameter-sparse empirical models that have been calibrated and/or fitted against contemporary measurements,we assessed China’s terrestrial carbon sequestration over 2010-2060 and its contribution to offsetting national energy-related CO_(2) emissions.We show that terrestrial C sequestration in China will increase from 0.375±0.056(mean±standard deviation)Pg C yr^(-1) in the 2010s to 0.458±0.100 Pg C yr^(-1)under RCP2.6 and 0.493±0.108 Pg C yr^(-1) under the RCP4.5 scenario in the 2050s,when TOMs are implemented.The majority of carbon sequestration comes from forest,accounting for 67.8-71.4%of the total amount.China’s terrestrial ecosystems can offset 12.2-15.0%and 13.4-17.8%of energy-related peak CO_(2) emissions in 2030 and 2060,respectively.The implementation of TOMs contributes 11.9%of the overall terrestrial carbon sequestration in the 2020s and 23.7%in the 2050s.The most likely strategy to maximize future NCS effectiveness is a full implementation of all applicable cost-effective NCS pathways in China.Our findings highlight the role of terrestrial carbon sequestration in offsetting energy-related CO_(2) emissions and put forward future needs in the context of carbon neutrality. | Yao Huang Wenjuan Sun Zhangcai Qin Wen Zhang Yongqiang Yu Tingting Li Qing Zhang Guocheng Wang Lingfei Yu Yijie Wang Fan Ding Ping Zhang | 2022 | National Science Review2022，9，8： | 4 |
| 4 | Proliferation and gene expression of osteoblasts cultured in DMEM containing the ionic products of dicalcium silicate coating显示文摘 | Junying Sun Jianyou Li Xuanyong Liu Li Wei Guocheng Wang Fanhao Meng | 2009 | Biomedicine & Pharmacotherapy2009，，9： | 1 |
| 5 | ITO electrode modified by self-assembly multilayer film of polyoxometallate on poly( vinyl alcohol) nanofibers and its electrocatalytic behavior显示文摘 | Shan Yuping Yang Guocheng Sun Yongling | 2007 | Electrochimica Acta2007，53，： | 1 |
| 6 | Experimental research on adsorption/desorption for petroleum pollutants in the soil in Daqing oilfield显示文摘 | Xiaoyan LIU Yingli LI Guocheng MAO Jungang LI Chun LI Jingxin SUN Qianya ZHU | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 1 |
| 7 | Application ofantigen sensitized DC and CIK in the treatment of primary liver cancer 显示文摘 | Zhong Guocheng Zhang Xiaoyu Sun Yi | 2009 | Chinese Journal of Clinical Oncology2009，36，24： | 1 |
| 8 | The differences of osmotic behaviors of bovine embryos from in vitro and in vivo and their quality evaluation显示文摘 | Jiang Guocheng Sun Yanxiang Yang Zhonghua | 1997 | Theriogenology1997，47，1： | 1 |
| 9 | The differences of osmotic behaviors of bovine embryos from invitro and in vivo and their quality evaluation显示文摘 | Jiang Guocheng Sun Yanxiang Yang Zhonghua | 1997 | Theriogenology1997，47，1： | 1 |
| 10 | Most root-derived carbon inputs do not contribute to long-term global soil carbon storage显示文摘Plant root-derived carbon(C)inputs(I_(root))are the primary source of C in mineral bulk soil.However,a fraction of I_(root)may lose quickly(I_(loss),e.g.,via rhizosphere microbial respiration,leaching and fauna feeding)without contributing to long-term bulk soil C storage,yet this loss has never been quantified,particularly on a global scale.In this study we integrated three observational global data sets including soil radiocarbon content,allocation of photo synthetically assimilated C,and root biomass distribution in 2,034 soil profiles to quantify I_(root)and its contribution to the bulk soil C pool.We show that global average I_(root)in the 0-200 cm soil profile is 3.5 Mg ha^(-1)yr^(-1),~80%of which(i.e.,I_(loss))is lost rather than co ntributing to long-term bulk soil C storage.I_(root)decreases exponentially with soil depth,and the top 20 cm soil contains>60%of total I_(root).Actual C input contributing to long-term bulk soil storage(i.e.,I_(root)-I_(loss))shows a similar depth distribution to I_(root).We also map I_(loss)and its depth distribution across the globe.Our results demonstrate the global significance of direct C losses which limit the contribution of I_(root)to bulk soil C storage;and provide spatially explicit data to facilitate reliable soil C predictions via separating direct C losses from total root-derived C inputs. | Guocheng WANG Liujun XIAO Ziqi LIN Qing ZHANG Xiaowei GUO Annette COWIE Shuai ZHANG Mingming WANG Songchao CHEN Ganlin ZHANG Zhou SHI Wenjuan SUN Zhongkui LUO | 2023 | Science China Earth Sciences2023，66，5： | 0 |
| 11 | Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells显示文摘Objective:This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell(hucMSC)-derived exosomes(hucMSC-Exos)in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A(VEGF-A)in immature human fetal retinal microvascular endothelial cells(hfRMECs).Methods:Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques,including transmission electron microscopy,nanoparticle tracking analysis,bicinchoninic acid assays,and western blotting.The hfRMECs were identified using von Willebrand factor(vWF)co-staining and divided into four groups:a control group cultured under normoxic condition,a hypoxic model group,a hypoxic group treated with low-concentration hucMSC-Exos(75μg/mL)and a hypoxic group treated with high-concentration hucMSC-Exos(100μg/mL).Cell viability and proliferation were assessed using Cell Counting Kit-8(CCK-8)assay and EdU(5-ethynyl-2′-deoxyuridine)assay respectively.Expression levels of VEGF-A were evaluated using RT-PCR,western blotting and immunofluorescence.Results:Hypoxia significantly increased hfRMECs’viability and proliferation by upregulating VEGF-A levels.The administration of hucMSC-Exos effectively reversed this response,with the high-concentration group exhibiting greater efficacy compared to the lowconcentration group.Conclusion:In conclusion,hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells. | JING LI WANWAN FAN LILI HAO YONGSHENG LI GUOCHENG YU WEI SUN XIANQIONG LUO JINGXIANG ZHONG | 2023 | BIOCELL2023，47，11： | 0 |
| 12 | Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst显示文摘Deoxycholic acid(DCA)has been authorized by the Federal Drug Agency for cosmetic reduction of redundant submental fat.The hydroxylated product(6β-OH DCA)was developed to improve the solubility and pharmaceutic properties of DCA for further applications.Herein,a combinatorial catalytic strategy was applied to construct a powerful Cytochrome P450 biocatalyst(CYP107D1,OleP)to convert DCA to 6β-OH DCA.Firstly,the weak expression of OleP was significantly improved using pRSFDuet-1 plasmid in the E.coli C41(DE3)strain.Next,the supply of heme was enhanced by the moderate overexpression of crucial genes in the heme biosynthetic pathway.In addition,a new biosensor was developed to select the appropriate redox partner.Furthermore,a cost-effective whole-cell catalytic system was constructed,resulting in the highest reported conversion rate of 6β-OH DCA(from 4.8%to 99.1%).The combinatorial catalytic strategies applied in this study provide an efficient method to synthesize high-value-added hydroxylated compounds by P450s. | Chixiang Sun Baodong Hu Yanchun Li Zhimeng Wu Jingwen Zhou Jianghua Li Jian Chen Guocheng Du Xinrui Zhao | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
| 13 | Agrobacterium mediated,construction of rice mutant population with transposon显示文摘Transposon tagging is a powerful tool for gene study, especially for genes among different plants, such as Arabidopsis, tobacco, and tomato, etc. This effective strategy for gene isolation has been applied to rice and progress has been made in recent years. | ZHU Zhengge Fu Yaping Si Huamin Hu Guocheng Yu Yonghong SUN Zongxiu Key Lab for Rice Biology,Ministry of Agri,CNRRI,Hangzhou 310006,China | 2001 | Chinese Rice Research Newsletter2001，9，4： | 0 |
| 14 | Multi-Scale Fusion Algorithm for AUVs Integrated Navigation Systems显示文摘To deal with the low location accuracy issue of existing underwater navigation technologies in autonomous underwater vehicles(AUVs),a distributed fusion algorithm which combines the model's analysis method with a multi-scale transformation method is proposed for integrated navigation system based on AUV.First,integrated navigation system theory and system error sources are introduced in details.Secondly,a navigation system's observation equation on the original scale is decomposed into different scales by the discrete wavelet transform method,and noise reduction is performed by setting the wavelet de-noising threshold.At last,the dynamic equation and observation equations are fused on different scales by the wavelet transformation and Kalman filter.The results show that the proposed algorithm has smaller navigation error and higher navigation accuracy. | Yushan Sun Fanyu Wu Yuqi Wang Guocheng Zhang Bin Kong | 2019 | Journal of Beijing Institute of Technology2019，28，4： | 0 |
| 15 | Expert Consensus on Nutritional Support for Children with Congenital Heart Disease(2023 Edition)显示文摘The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the results of multi-center clinical nutrition research for congenital heart disease following thefirst Chinese consensus edition of 2016.The consensus was also shaped by the results of three discussion sessions and two questionnaires con-ducted by the 13-member collaboration group.This process was informed by both clinical guidelines and expert consensus.The quality of literature,both in English and Chinese,and the level of recommendations were evaluated using the Grading of Recommendations Assessment,Development,and Evaluations(GRADE)system. | Xuming Mo Wei Cai Jirong Qi Zhuoming Xu Ying Wang Weihui Yan Shoujun Li Nianguo Dong Xinxin Chen Jinfen Liu Qiang Shu Jimei Chen Haibo Zhang Hao Zhang Quansheng Xing Qi An Xiaofeng Li Xu Wang Yan He Junwu Su Taibing Fan Teng Ming Weibing Tang Li Hong Jinghao Zheng Ming Ye Guocheng Sun Yiqun Ding Liang Tao Yifeng Yang Zhongshi Wu Hua Cao Qiang Wang Keming Yang Libing Zhang Ping Wen Yanqin Cui Bo Zhai Yong Zou Qingya Tang Rui Chen Chun Wu Zhiyu Feng Caixia Liu Yaping Mi Rufang Zhang Ke Lin Xin Li Mingan Pi Xiangming Fan Shanshan Shi Peng Huang Zhengxia Pan Jiafeng Qi Renwei Chen Shuguang Tao Yaqin Shu Huifeng Zhang Lan Jiang Min Da Nishant Patel Liang Hu Cardiac Surgery Group of Pediatric Surgery Society of Chinese Medical Association and Parenteral Enteral Nutrition Society of Chinese Medical Association | 2023 | Congenital Heart Disease2023，18，6： | 0 |
| 16 | Chitin deacetylase:from molecular structure to practical applications显示文摘Chitosan and chitooligosaccharides(COS),as derivatives of chitin through deacetylation reaction,have broad applications due to their good biodegradability,biocompatibility,and solubility.In addition,chitosan and COS are involved in cell wall morphogenesis and host–pathogen interactions in vivo.Chitin deacetylases(CDAs)are enzymes that can catalyze the deN-acetylation of chitin.They are widely distributed in protozoa,algae,bacteria,fungi,and insects with important physiological functions.Compared with the traditional chemical method,enzymatic catalysis by CDAs provides an enzymatic catalysis method to produce chitosan and COS with controllable deacetylation site and environmental friendliness.These characteristics attract researchers to produce CDAs by fungicides or pesticides.However,researches on heterologous expression and directed evolution of CDAs are still lacking.In this review,we summarize the latest knowledge of CDAs,especially for heterologous expression systems and directed evolution strategies,which may contribute to the industrial production and future application of CDAs. | Ziyang Huang Xueqin Lv Guoyun Sun Xinzhu Mao Wei Lu Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2022 | Systems Microbiology and Biomanufacturing2022，2，2： | 0 |
| 17 | Advances on bioremediation of oil-contaminated soil in cold region显示文摘 | Xiaoyan LIU Jingxin SUN Guocheng MAO Chunlei DAI Chun LI Qianya ZHU Yingli LI | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 0 |
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