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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | Maspin expression and its clinicopathological significance in tumorigenesis and progression of gastric cancer显示文摘AIM:To investigate maspin expression in tumorigenesis and progression of gastric cancer and to explore its relevant molecular mechanisms.METHODS:Formalin-fixed and paraffin-embedded tissues from normal mucosa (n=182), dysplasia (n=69), cancer (n=l13) of the stomach were studied for maspin expression by immunohistochemistry. Microvessel density (MVD) in gastric cancer was labeled using anti-CD34 antibody. Maspin expression was compared with clinical parameters and MVD of tumors. Caspase-3 expression was also detected in gastric carcinoma by immunohistochemistry. The relationship between Caspase-3 and maspin expression was concerned as well.RESULTS:The positive rates of maspin expression were 79.8% (145/182), 75.4%(52/69) and 50.4%(57/113) in normal mucosa, dysplasia and cancer of the stomach,respectively.Cancer less frequently expressed maspin than normal mucosa and dysplasia (P<0.05).Maspin expression showed a significantly negative association with invasive depth, metastasis, Lauren's and Nakamura's classification (P<0.05),but not with tumor size, Borrmann's classification,growth pattern or TNIVl staging (P>0.05). The positive rate of Caspase-3 was significantly lower in gastric cancer than in normal gastric mucosa (P<0.05,32.7% vs 50.4%). It was noteworthy that maspin expression was negatively correlated with MVD, but positively correlated with expression of Caspase-3 in gastric cancer (P<0.05).CONCLUSION:Down-regulated maspin expression is a late molecular event in gastric carcinogenesis. Reduced expression of maspin contributes to progression of gastric cancer probably by inhibiting cell adhesion, enhandng cell mobility,decreasing cell apoptosis and facilitating angiogenesis.Additionally altered expression of maspin underlies the molecular mechanism of differentiation of gastric cancer and supports the different histogenetic pathways of intestinal and diffuse gastric cancers. Maspin expression can be considered as an effective and objective marker to reveal biological behaviors of gastric cancer. | Meng-ChunWang Yan-MinYang Xiao-HanLi FangDong YanLi | 2004 | World Journal of Gastroenterology2004，10，5： | 33 |
| 3 | Subcellular distribution and chemical form of Pb in hyperaccumulator Arenaria orbiculata and response of root exudates to Pb addition显示文摘答案文化被进行以便在 Arenaria orbiculata 理解累积特征和 Pb 的化学形式(一。orbiculata ) 并且到 Pb 增加的根流出物的反应。结果显示出那:1 ) 在 A 的射击和根的 Pb 内容。orbiculata 与在答案在 Pb 集中增加增加了。2 ) 在 Pb 增加下面的 Pb 化学形式的内容是列在后面: HAc 可引出的部分( F <潜水艇class=“ a-plus-plus ”> HAC )> HCl 可引出的部分( F <潜水艇class=“ a-plus-plus ”> HCl )> NaCl 可引出的部分( F <潜水艇class=“ a-plus-plus ”> NaCl )>乙醇可引出的部分( F <潜水艇class=“ a-plus-plus ”> E )>浇可引出的部分( F <潜水艇class=“ a-plus-plus ”> W )。3 ) 在引起的媒介的增加的 Pb 水平在射击和根的四 subcellular 部分在 Pb 内容增加,与在 FIV 的大多数累积(部分 IV,可溶的部分) 在射击和 FI (部分我,房间墙部分) 在根。4 ) 可溶的糖和根流出物的免费氨基酸的内容与在溶液增加 Pb 集中增加了。在可溶的糖和免费氨基酸的 Pb 和内容之间的显著地积极的关联被观察。5 ) 与在答案的 Pb 集中,低分子的重量器官的酸(LMWOA ) 内容跟随了趋势:酒石的酸 > 醋酸 > 苹果酸酸 > 柠檬性的酸。显著地积极的关联在 Pb 和柠檬性的酸内容之间被观察。结果显示在 A.orbiculata 的根流出物的可溶的糖,免费氨基酸和柠檬性的酸便于 Pb 的吸收和累积,它在 NaCl- , HCl- 和 HAc- 存在可引出的 Pb 形式, FI 和 FIV 部分,导致 A.orbiculata 的忍耐到 Pb。 | Yanqun ZU Yuan LI Huan MIN Fangdong ZHAN Li QIN Jixiu WANG | 2015 | Frontiers of Environmental Science & Engineering2015，9，2： | 6 |
| 4 | Intercropping of Sonchus asper and Vicia faba affects plant cadmium accumulation and root responses显示文摘The cadmium(Cd)pollution of farmland soil is serious in the world.The present study investigated the effects of intercropping Vicia faba and the hyperaccumulator Sonchus asper on the Cd accumulation and root responses(morphology and secreted organic acids)of plants grown on soil from a mining area in Yunnan Province,China,under different Cd stress levels(0,50,100,and 200 mg kg-1).Intercropping increased the biomass of both S.asper and V.faba,as well as the Cd accumulation and Cd transfer coefficient from roots to shoots of S.asper,but decreased those of V.faba in the 200 mg kg-1Cd treatment.The Cd concentrations in roots,shoots,and grains from intercropped V.faba plants were positively correlated(P<0.05)with the N,N-diethyl phenylacetamide-extractable Cd content in soil.In the 50 mg kg-1Cd treatment,intercropping decreased citric and malic acids in root secretions of S.asper.Intercropped V.faba secreted more citric,oxalic,acetic,and malic acid compared with S.asper.Intercropping also increased root length and root surface area of both S.asper and V.faba.At 50 mg kg-1Cd,root length for S.asper was higher than that at 0 mg kg-1Cd,whereas V.faba roots had significantly decreased length and mean diameter.Intercropping of S.asper and V.faba is suggested as an in situ phytoremediation strategy of Cd-contaminated soils and may improve the food quality of V.faba. | Yanqun ZU Li QIN Fangdong ZHAN Jiong WU Yuan LI Jianjun CHEN Jixiu WANG Wenyou HU | 2020 | Pedosphere2020，30，4： | 6 |
| 5 | Construction of a bacterial artificial chromosome library of S-type CMS maize mitochondria显示文摘In order to isolate mitochondrial genes easily, we have developed a new method to construct S-type CMS maize mitochondrial gene library by means of embedding mitochondria and enzymatic digesting mitochondria in situ, preparing mtDNA by electrophoresis, digesting LMP agarose with β-agarase, using BAC vector and electroporation. About 2 500 white clones of Mo17 CMS-J mitochondrial gene library were obtained with the average size of 18.24 kb, ranging from 5 to 40 kb, 63.6% inserts came from mitochondrial genome and represented 48 × mitochondrial genome equivalents. All the probes had detected the positive clones in the gene library. It is helpful to elucidating the maize mitochondrial genome structure and mechanism of S-type CMS, and may give some valuable reference to the construction of other plant mitochondrial genome library. | Fangdong Zhang Yonglian Zheng Zhigang Cao | 2000 | Chinese Science Bulletin2000，45，18： | 5 |
| 6 | Electroporation: A New Approach Enhancing Antitumor Effects of Cytoxan显示文摘Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (EPs) inducing cell membrane pored (electroporation) are used as a means of delivering antitumor drugs to the cytoplasm of cancer cells. In vitro, with scan electromicroscope (SEM) and Trypan blue staining examination, the best parameter of EPs of electroporation is studied by the S-180 cells exposed to EP with various voltages, pulses , capacitance. The best parameter of EP of electroporation is 600V/cm, 6 pulses, 10 μF. In the in vivo study, ECT is studied with the Cytoxan (CTX) injected directly into the tumor followed immediately by a local EP at the tumor site. Four parameters, which include the tumor inhibitory ratio, the curing ratio and the vas capillare of tumor, the tumor’s histopathological characteristics are determined and compared among the ECT group, the control group, the EP-only group and the drug-only group. The results indicate that the antitumor effect of CTX is significantly enhanced by electroporation. | 杨孔 YUE Bisong Wang Zishu ZOU Fangdong Zhao Ermi WANG Baoyi Zhang Hong | 2003 | High Technology Letters2003，9，3： | 5 |
| 7 | Design of an Enhanced Sensitivity FBG Strain Sensor and Application in Highway Bridge Engineering显示文摘紧张传感器被给,并且而且高取得资格,拉紧传感器的提高的敏感纤维栅栏(FBG ) 的理论设计方法被开发并且制作,谁的察觉到性质对实际应用程序好。有圆柱的壳封装的紧张传感器包含了三管状的结构,由于不平的表面结构,在紧张集中的区域,改进敏感。它能完成埋入紧张测量和表面测量并且有容易的安装的优点。好协议在大小和理论模拟结果之间被获得。在每刻度测试以后, 24 个 FBG 紧张传感器和六个 FBG 温度赔偿传感器在 Yongtaiwen 高速公路在 Diaoshuiyan 桥牌的盒子 girder 的下面上被安装了。最后,我们为高速公路桥建立了一个长期的结构健康系统。 | Litong LI Dongsheng ZHANG Hui LIU Yongxing GUO Fangdong ZHU | 2014 | Photonic Sensors2014，4，2： | 4 |
| 8 | Hierarchical Octahedral NazMnFe(CN)6 and Na2MnFe(CN)6@Ppy as Cathode Materials for Sodium-Ion Batteries显示文摘Na 2 MnFe (CN )6 被一个简单一起沉淀方法成功地综合,然后与在 Na 2 MnFe (CN )6 粒子。有趣地, Na 2 MnFe (CN )6 和 Na 2 两个显示出的 MnFe (CN )6@PPy 有纳米大小的子单元的空八面的层次结构。Na 2 MnFe (CN )6@PPy 在 10 杂志 1 的当前的密度在 150 个周期以后展出 107 mAhg 1 的一个分泌物能力。它能仍然是 65 mAhg 1 甚至当前的密度被增加到 200 杂志 1 。这个优异电气化学的性质能象改进电子电导率一样被归功于到唯一的层次建筑学。 | Fangdong Hu Lin Li Xiaolei Jiang | 2017 | Chinese Journal of Chemistry2017，35，4： | 2 |
| 9 | Validation of an operational forecasting system of sea dike risk in the southern Zhejiang Province, South China显示文摘In this study,an operational forecasting system of sea dike risk in the southern Zhejiang Province,South China was developed based on a coupled storm-surge and wave model.This forecasting system is important because of the high cost of storm-surge damage and the need for rapid emergency planning.A comparison with astronomical tides in 2016 and the validation of storm surges and high water marks of 20 typhoons verified that the forecast system has a good simulation ability.The system can forecast relatively realistic water levels and wave heights as shown under the parametric atmospheric forces simulated in a case study;the sea dikes in credible high risk were mainly located in the estuaries,rivers,and around the islands in the southern Zhejiang.Therefore,the forecast system is applicable in the southern Zhejiang with a support to the effective prevention from typhoon storm-surge damage. | LI Tao WANG Fangdong HOU Jingming CHE Zhumei DONG Jianxi | 2019 | Journal of Oceanology and Limnology2019，37，6： | 2 |
| 10 | Differential SW16.1 allelic effects and genetic backgrounds contributed to increased seed weight after soybean domestication显示文摘Although seed weight has increased following domestication from wild soybean(Glycine soja) to cultivated soybean(Glycine max), the genetic basis underlying this change is unclear. Using mapping populations derived from chromosome segment substitution lines of wild soybean, we identified SW16.1 as the causative gene underlying a major quantitative trait locus controlling seed weight.SW16.1 encodes a nucleus-localized LIM domaincontaining protein. Importantly, the GsSW16.1 allele from wild soybean accession N24852 had a negative effect on seed weight, whereas the GmSW16.1 allele from cultivar NN1138-2 had a positive effect. Gene expression network analysis,reverse-transcription quantitative polymerase chain reaction, and promoter-luciferase reporter transient expression assays suggested that SW16.1 regulates the transcription of MT4, a positive regulator of seed weight. The natural variations in SW16.1 and other known seed weight genes were analyzed in soybean germplasm. The SW16.1 polymorphism was associated with seed weight in 247 soybean accessions, showing much higher frequency of positive-effect alleles in cultivated soybean than in wild soybean. Interestingly,gene allele matrix analysis of the known seed weight genes revealed that G. max has lost 38.5%of the G. soja alleles and that most of the lost alleles had negative effects on seed weight. Our results suggest that eliminating negative alleles from G. soja led to a higher frequency of positive alleles and changed genetic backgrounds in G. max,which contributed to larger seeds in cultivated soybean after domestication from wild soybean.Our findings provide new insights regarding soybean domestication and should assist current soybean breeding programs. | Xianlian Chen Cheng Liu Pengfei Guo Xiaoshuai Hao Yongpeng Pan Kai Zhang Wusheng Liu Lizhi Zhao Wei Luo Jianbo He Yanzhu Su Ting Jin Fenfen Jiang Si Wang Fangdong Liu Rongzhou Xie Changgen Zhen Wei Han Guangnan Xing Wubin Wang Shancen Zhao Yan Li Junyi Gai | 2023 | Journal of Integrative Plant Biology2023，65，7： | 1 |
| 11 | Charac-terization of melanin isolated from a dark septate endophyte (DSE),Exophiala pisciphila显示文摘 | Zhan Fangdong He Yongmei Zu Yanqun | | 0，，10： | 1 |
| 12 | Impact of the Qinghai-Tibet Railway on Population Genetic Structure of the Toad-Headed Lizard,Phrynocephalus vlangalii显示文摘Using data from nine microsatellite DNA loci and a population genetic approach,we evaluate the barrier effect of the Qinghai-Tibet Railway on toad-headed lizard,Phrynocephalus vlangalii. The study area is along a 20 km stretch of the railway on northern Qinghai-Tibet Plateau,and this section of the railway was constructed between 1958–1979. Both assignment tests and analysis of molecular variance(AMOVA) were used for data analysis. We found significant genetic differentiation between the populations from the study area and those from a further southeastern area,which are separated by a 20 km gap. This suggests the existence of population substructure at a fine-scale. However,we did not detect any difference between samples from the western and eastern sides of the railway within the study area,and concluded that the railway may not impose a significant barrier effect on these lizard populations at the present time. Available suitable habitat alongside the railway and bridge underpasses may have facilitated the gene exchange between the sides. The relatively short time since the completion of the railway may not allow the differentiation to accumulate to a detectable level. Since the Qinghai-Tibet Plateau maintains a unique and fragile ecosystem,long-term monitoring of such man-made landscape features is imperative for protecting this ecosystem. | Dun HU Jinzhong FU Fangdong ZOU Yin QI | 2012 | Asian Herpetological Research2012，3，4： | 1 |
| 13 | Light distribution, pho- tosynthetic rate and yield in a Paulownia-wheat intercropping sys- tem in China显示文摘 | Fangdong Li Ping Meng Dali Fu | 2008 | Agriforestry Systems2008，74，： | 1 |
| 14 | Phylogenetic and molecular dating analysis of Taiwan Blue Pheasant ( Lophura swinhoii )显示文摘 | Lichun Jiang Gaochao Wang Rui Peng Quekun Peng Fangdong Zou | 2014 | Gene2014，，： | 1 |
| 15 | A novel silvicultural model for increasing biopolymer production from Eucommia ulmoides Oliver trees显示文摘 | Zhiqiang Sun Fangdong Li Hongyan Du Jingle Zhu Y.P. Wang | 2013 | Industrial Crops & Products2013，，： | 1 |
| 16 | Directing group-assisted transition-metal-catalyzed vinylic C–H bond functionalization显示文摘Transition-metal-catalyzed C–H bond activation represents one of the most attractive research areas in organic synthesis.In contrast to the great developments made in directed C–H bond functionalization of arenes,the directing group-assisted activation of non-aromatic vinylic C–H bonds still remains challenging.During the recent years,significant progress has been made in this fascinating field with various functionalized alkenes,heterocycles and carbocycles being obtained.This article will focus on the recent achievements in the field of directing-group-assisted vinylic C–H bond functionalization. | Kang Wang Fangdong Hu Yan Zhang Jianbo Wang | 2015 | Science China Chemistry2015，58，8： | 1 |
| 17 | Light distribution, photosynthetic rate and yield in a Paulownia- wheat intercropping system in China 显示文摘 | Fangdong Li Ping Meng Dali Fu Baoping Wang | 2008 | Agroforestry Systems2008，74，2： | 1 |
| 18 | Molecular cloning and sequence analysis of interferon-gamma and interleukin-6 from Tibetan macaque (Maeaca thibetana)显示文摘 | Wei Kun Liang Xu Zou Fangdong | 2006 | Veterinary Immunology and Immunopathology2006，114，34： | 1 |
| 19 | Isolation and characterization of microsatellite loci from forest musk deer(Moschus berezovskii)显示文摘 | Fangdong Zou Bisong Yue Liu Xu | 2005 | Zoological Science2005，22，： | 1 |
| 20 | Detector Research Condition Project显示文摘The objective of the detector laboratory researching conditions development project is to build part of the experimental conditions of the semiconductor process,which belongs to the construction project of the nuclear base.The construction contents include about 450 m^2 clean laboratory(including 1 000level clean | YANG Fangdong | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 0 |
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