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| 1 | Ellagic acid induces apoptosis through inhibition of nuclear factor κB in pancreatic cancer cells显示文摘AIM:To determine the effect of ellagic acid on apop-tosis and proliferation in pancreatic cancer cells and to determine the mechanism of the pro-survival effects of ellagic acid. METHODS:The effect of ellagic acid on apoptosis was assessed by measuring Phosphatidylserine externalization,caspase activity,mitochondrial membrane potential and DNA fragmentation;and proliferation by measuring DNA thymidine incorporation. Mitochondrial membrane potential was measured in permeabilized cells,and in isolated mitochondria. Nuclear factor kB(NF-kB) activity was measured by electromobility shift assay(EMSA) . RESULTS:We show that ellagic acid,a polyphenolic compound in fruits and berries,at concentrations 10 to 50 mmol/L stimulates apoptosis in human pancreatic adenocarcinoma cells. Further,ellagic acid decreases proliferation by up to 20-fold at 50 mmol/L. Ellagic acid stimulates the mitochondrial pathway of apoptosis associated with mitochondrial depolarization,cytochrome C release,and the downstream caspaseactivation. Ellagic acid does not directly affect mitochondria. Ellagic acid dose-dependently decreased NF-kB binding activity. Furthermore,inhibition of NF-kB activity using IkB wild type plasmid prevented the effect of ellagic acid on apoptosis. CONCLUSION:Our data indicate that ellagic acid stimulates apoptosis through inhibition of the prosu-rvival transcription factor NF-kB. | Mouad Edderkaoui Irina Odinokova Izumi Ohno Ilya Gukovsky Vay Liang W Go Stephen J Pandol Anna S Gukovskaya | 2008 | World Journal of Gastroenterology2008，14，23： | 30 |
| 2 | XIAP抑制剂Embelin体外对人胰腺癌细胞株的抑制效应与机制研究显示文摘目的探讨XIAP小分子抑制剂Embelin体外对人胰腺癌细胞株MIAPaCa-2的抑制效应与机制。方法将生长至第4-12代之间的MIAPaca-2细胞分为DMSO对照组与Embelin实验组,在实验组细胞中加入不同浓度的Embelin共孵育72h,收集细胞并裂解后取上清液用细胞凋亡试剂盒检测组蛋白(凋亡指标)的OD值水平。MIAPaca-2细胞加入不同浓度的Embelin共孵育60h后再加入3H胸腺嘧啶继续孵育12h,取细胞裂解液用同位素γ闪烁计数仪检测3H-CPM数值以判断细胞增殖程度。对DMSO对照组与Embelin实验组的组蛋白OD值与3H-CPM数值分别进行比较分析。结果Embelin实验组的MIAPaCa-2细胞株组蛋白OD值显著高于DMSO对照组而3H-CPM数值明显低于DMSO对照组(P<0.01),且实验组Embe-lin的作用浓度与组蛋白OD值呈正相关(r=0.996,P<0.01),而与3H-CPM数值呈负相关(r=-0.993,P<0.01)。结论Embelin呈剂量依赖性地诱导人胰腺癌细胞株MIAPaCa-2凋亡并抑制其增殖,有肿瘤抑制效应。 | 郑玲 吕寒静 Mouad Edderkaoui Stephen J Pandol | 2009 | 山西医科大学学报2009，40，1： | 4 |
| 3 | Ellagic acid and embelin affect key cellular components of pancreatic adenocarcinoma, cancer, and stellate ceils 显示文摘 | Edderkaoui M Lugea A Hui H | 2013 | Nutr Cancer2013，65，8： | 1 |
| 4 | The phosphatase PHLPP1 regulates Akt2,promotes pancreatic cancer cell death,and inhibits tumor formation 显示文摘 | Nitsche C Edderkaoui M Moore RM | 2012 | Gastroenterology2012，142，2： | 1 |
| 5 | Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells显示文摘 | Vaquero EC Edderkaoui M Pandol SJ Gukovasky I | 2004 | J Biol Chem2004，279，34： | 1 |
| 6 | Tumor necrosis factor-alpha mediates pancreatitis responses in acinar cells via protein kinase C and proline-rich tyrosine kinase 2显示文摘 | Satoh A Gukovskaya A S Edderkaoui M | | Gastroenterology0，，： | 1 |
| 7 | Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells显示文摘 | Vaquero EC Edderkaoui M Pandol SJ | 2004 | J Biol Chem2004，279，34： | 1 |
| 8 | The phos- phatase PHLPP1 regulates Akt2, promotes pancreatic cancer cell death, and inhibits tumor formation 显示文摘 | Nitsche C Edderkaoui M Moore R M | 2012 | Gastroenterology2012，142，2： | 1 |
| 9 | Reactive oxygen species produced by NAD (P)H oxidase inhibit apoptosis in pancreatic cancer cells 显示文摘 | Vaquero EC Edderkaoui M Pandol S J | 2004 | J Biol Chem2004，279，34： | 1 |
| 10 | The phosphatase PHLPP1 regulates Akt2, promotes pancreatic cancer cell death, and inhibits tumor formation 显示文摘 | Nitsche C Edderkaoui M Moore RM | 2012 | Gastroenterology2012，142，2： | 1 |
| 11 | Tumor necrosisfactor-alpha mediates pancreatitis responses in acinar cells viaprotein kinase C and proline-rich tyrosine kinase 2 显示文摘 | Satoh A Gukovskaya AS Edderkaoui M | 2005 | Gastroenterology2005，129，2： | 1 |
| 12 | NADPH oxidase acti- vation in pancreatic cancer cells is mediated through Akt-de- pendent up-regulation of p22phox 显示文摘 | Edderkaoui M Nitsche C Zheng L | 2013 | J Biol Chem2013，288，36： | 1 |
| 13 | Insulin-like growth factor-Ⅰ receptor mediates the prosurvival effect of fibronectin显示文摘 | Edderkaoui M Hong P Lee J K | 2007 | J Biol Chem2007，282，26： | 1 |
| 14 | Ellagic Acid and Embelin Affect Key Cellular Components of Pancreatic Adenocarcinoma, Cancer, and Stellate Cells显示文摘 | Mouad Edderkaoui Aurelia Lugea Hongxiang Hui Guido Eibl Qing-Yi Lu Aune Moro Xuyang Lu Gang Li Vay-Liang Go StephenJ. Pandol | 2013 | Nutrition and Cancer2013，，8： | 1 |
| 15 | The phos- phatase PHLPP1 regulates Akt2, promotes pancreatic cancer cell death, and inhibits tumor formation显示文摘 | Nitsche C Edderkaoui M Moore RM | 2012 | Gas- troenterology2012，142，2： | 1 |
| 16 | Epidemiolo- gy, risk factors, and the promotion of pancreatic cancer= role of the stellate cell显示文摘 | Pandol S Gukovskaya A Edderkaoui M | 2012 | J Gastroenterol Hepatol2012，27，2： | 1 |
| 17 | Risk factors for pancreatic cancer: underlying mechanisms and potential targets 显示文摘 | Edderkaoui M Eibl G | 2014 | Front Physiol2014，5，： | 1 |
| 18 | Tumor necrosis factor - alpha mediates pancreatitis responses in acinar cells via protein kinase C and proline - rich tyrosine kinase 2 显示文摘 | Satoh A Gukovskaya AS Edderkaoui M | 2005 | Gas- troenterology2005，129，2： | 1 |
| 19 | Reactive oxygenspecies produced by NAD (P)H oxidase inhibit apoptosis in pancreatic cancer cells 显示文摘 | Vaquero EC Edderkaoui M Pandol S J | 2004 | J Biol Chem2004，279，34： | 1 |
| 20 | Desmoplasia of pancreatic ductal adenocarcinoma显示文摘 | Pandol S Edderkaoui M Gukovsky I | | 0，，： | 1 |
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