|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells显示文摘Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a microsphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2012 | Science China(Life Sciences)2012，55，11： | 16 |
| 2 | The Synthesis of a Stewart Platform for a Specific Workspace with a Genetic Algorithm显示文摘The synthesis problem of a 6-SPS Stewart platform with semi-regular hexagons is studied in this paper. We present a genetic algorithm approach to synthesize a Stewart platform whose workspace must include a desired workspace with orienting capabilities in a given 3D region. Based on the observation that the constant orientation workspace gets smaller as the given orientations increase, a simple method is presented to describe the workspace with orienting capabilities, which need only search the constant orientation workspaces with the eight sets of extremal orientations of given 3D region and use their common part.To verify the method, a numerical example is given. The GA is used to optimize architectural parameters and obtain a compact manipulator. The method is applied to synthesize a Stewart platform whose workspace must include a prescribed cylinder with orienting capabilities. | 陈学生 Chen Zaili Xie Tiao | 2004 | High Technology Letters2004，10，1： | 11 |
| 3 | 基于灰云模型的海上交通系统风险推理方法显示文摘引入灰云理论的映射方法定量推理海上交通系统风险。首先,提出风险事件成因发生的可能性及其影响程度,定量推理海上交通系统风险;其次,构建灰云模型应用流程,推理海上交通风险成因的作用效果;再次,借助云模型的代数运算和升云计算,得出海上交通系统风险;最后,结合我国某港口水域的船舶交通风险数据,推理船员、船舶和环境等3个因素作用下的海上交通系统风险。运用基于系统因素相互作用的风险二维云推理,得出港口水域船舶交通风险水平为0.3‰,且不稳定。算例应用结果表明:借助灰云模型有助于不确定描述与定常信息的量化转换,实现多因素作用下的海上交通安全风险推理。 | 龚慧佳 轩少永 胡甚平 YANG Zaili | 2016 | 中国航海2016，39，2： | 8 |
| 4 | Application of a collaborative modelling and strategic fuzzy decision support system for selecting appropriate resilience strategies for seaport operations显示文摘The selection of an appropriate resilience investment strategy to optimize the operational efficiency of a seaport is a challenging task given that many criteria need to be considered and modelled under an uncertain environment.The design of such a complex decision system consists of many subjective and imprecise parameters contained in different quantitative and qualitative forms.This paper proposes a fuzzy multi-attribute decision making methodology for the selection of an appropriate resilience investment strategy in a succinct and straightforward manner.The decision support model allows for a collaborative modelling of the system by multiple analysts in a group decision making process.Fuzzy analytical hierarchy process(FAHP) was utilized to analyse the complex structure of the system to obtain the weights of all the criteria while fuzzy technique for order of preference by similarity to ideal solution(TOPSIS) was employed to facilitate the ranking process of the resilience strategies.Given that it is often financially difficult to invest in all the resilience strategies,it is envisaged that the proposed approach could provide decision makers with a flexible and transparent tool for selecting appropriate resilience strategies aimed at increasing the resilience of seaport operations. | Andrew John Zaili Yang Ramin Riahi Jin Wang | 2014 | Journal of Traffic and Transportation Engineering(English Edition)2014，1，3： | 6 |
| 5 | Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy显示文摘Atomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 × 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Chinese Science Bulletin2013，58，13： | 6 |
| 6 | Atomic force microscopy imaging of live mammalian cells显示文摘Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Science China(Life Sciences)2013，56，9： | 6 |
| 7 | Progress of graphene growth on copper by chemical vapor deposition:Growth behavior and controlled synthesis显示文摘Recently,chemical vapor deposition (CVD) on copper has been becoming a main method for preparing large-area and highquality monolayer graphene.In this paper,we first briefly introduce the preliminary understanding of the microstructure and growth behavior of graphene on copper,and then focus on the recent progress on the quality improvement,number of layers control and transfer-free growth of graphene.In the end,we attempt to analyze the possible development of CVD growth of graphene in future,including the controlled growth of large-size single-crystal graphene and bilayer graphene with different stacking orders. | MA LaiPeng REN WenCai DONG ZaiLi LIU LianQing CHENG HuiMing | 2012 | Chinese Science Bulletin2012，57，23： | 6 |
| 8 | Progress of AFM single-cell and single-molecule morphology imaging显示文摘Atomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallog-raphy. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Chinese Science Bulletin2013，58，26： | 5 |
| 9 | Research on the reconstruction of fast and accurate AFM probe model显示文摘The AFM image will be distorted due to the tip-broadening effect. It may be mathematically represented as the effect of the tip's convolution on the real image. As a result, the true geometry of the sample surface can not be obtained. If the tip shape could be known accurately, such distortion can be eliminated with the adoption of disconvolution operation. It is of practical significance in the accurate reconstruction of the AFM image. Among the current tip modeling algorithms, the blind tip estimation algorithm based on mathematical morphology is widely applied. However, it takes much time and has difficulties in defining the optimal noise threshold. In view of the above problems, this paper proposes a new blind tip evaluation method to accelerate the speed of blind tip modeling calculation and realize the accurate reconstruction of the AFM image. The simulation and the experimental results demonstrate the feasibility and effectiveness of the above research method. | YUAN Shuai DONG ZaiLi MIAO Lei XI Ning WANG YueChao | 2010 | Chinese Science Bulletin2010，55，24： | 4 |
| 10 | Friction anisotropy dependence on lattice orientation of graphene显示文摘The observation of friction anisotropy on graphene by friction measurement at atomic scale has been reported in this paper.Atomic-scale friction measurement revealed friction anisotropy with a periodicity of 60°,which is consistent with the hexagonal periodicity of the graphene.Both experiments and theory show that the value of the friction force is related to the graphene lattice orientation,and the friction force along armchair orientation is also larger than the one along zigzag orientation.These results will play a critical role in the use of graphene to manufacture nanoscale devices. | ZHANG Yu LIU LianQing XI Ning WANG YueChao DONG ZaiLi WEJINYA Uchechukwu C | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，4： | 4 |
| 11 | AFM based anodic oxidation and its application to oxidative cutting and welding of CNT显示文摘Probe anodic oxidation by atomic force microscope (AFM) is one of the most important techniques in fabricating nano structures and devices. The technique was further studied in this paper. By analyzing the distribution of the electric field on substrate surface the dependence of oxide characters on field was discussed. The impacts of various parameters on oxide fabrication were experimentally studied. Based on these studies, we realized the oxidative cutting and welding of carbon nanotube (CNT) by the AFM based oxidation technique and provided a novel technique for the assembly and fabrication of CNT based nano devices. | JIAO NianDong WANG YueChao XI Ning DONG ZaiLi | 2009 | Science China(Technological Sciences)2009，52，11： | 3 |
| 12 | Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy显示文摘The invention of atomic force microscopy(AFM) has provided new technology for measuring specific molecular interaction forces.Using AFM single-molecule force spectroscopy(SMFS) techniques,CD20-Rimximab rupture forces were measured on purified CD20 proteins,Raji cells,and lymphoma patient B cells.Rimximab molecules were linked onto AFM tips using AFM probe functionalization technology,and purified CD20 proteins were attached to mica using substrate functionalization technology.Raji cells(a lymphoma cell line) or lymphoma patient cells were immobilized on a glass substrate via electrostatic adsorption and chemical fixation.The topography of the purified CD20 proteins,Raji cells,and patient lymphoma cells was visualized using AFM imaging and the differences in the rupture forces were analyzed and measured.The results showed that the rupture forces between the CD20 proteins on Raji cells and Rituximab were markedly smaller than those for purified CD20 proteins and CD20 proteins on lymphoma patient B cells.These findings provide an effective experimental method for investigating the mechanisms underlying the variable efficacy of Rituximab. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi LI GuangYong XIAO XiuBin ZHANG WeiJing | 2011 | Chinese Science Bulletin2011，56，35： | 3 |
| 13 | Study on the large-scale assembly and fabrication method for SWCNTs nano device显示文摘The large-scale assembly and fabrication method for single-walled carbon nanotube(SWCNT) nano devices was implemented.Assembly of SWCNT field effect transistor(FET) was realized by floating potential dielectrophoresis approach.The simulation of floating potential distribution of the chip was performed by comsol multiphysics coupling software.Six hundred devices were assembled on the area of less than one square centimeter.The fabricated devices were characterized by atomic force microscopy and scanning electron microscopy.The experimental results showed that large-scale assembly had been realized,and the success rate of ideal assembly for SWCNT FET had been assessed. | XU Ke TIAN XiaoJun WU ChengDong LIU Jian LI MengXin DONG ZaiLi | 2013 | Science China Chemistry2013，56，3： | 3 |
| 14 | Research on the atomic force microscopy-based fabrication of nanochannels on silicon oxide surfaces显示文摘The atomic force microscopy (AFM)-based nanomachining of nanochannels on silicon oxide surfaces is investigated both theoretically and experimentally. The relationships of nanochannel depth versus cutting velocity,nanochannel depth versus normal force,friction force versus cutting velocity,and friction force versus normal force are systematically studied. Using the derived theory and fabrication method,a nanochannel with an expected depth can be machined simply by controlling the vertical deflection signal on the position sensitive detector of AFM. The theoretical analysis and fabrication method can be effectively used for AFM-based fabrication of nanochannels. | WANG ZhiQian JIAO NianDong TUNG Steve DONG ZaiLi | 2010 | Chinese Science Bulletin2010，55，30： | 3 |
| 15 | 人为因素对驾驶操作安全的影响评估显示文摘In recent years,marine pilotage accidents occurring on a worldwide basis as a result of human error have not been ceased to transpire,despite advances in technology and a significant set of international conventions,regulations,and recommendations to reduce them.This paper aims to investigate the effect of human factors on the safety of maritime pilotage operations.The human factors that affect the operators who are performing ships’berthing operations have also been examined in detail.In this study,in order to determine the causes of human-related errors occurred in maritime pilotage accidents,a comprehensive literature review is carried out,and a considerable number of real past case examples and an analysis of the maritime accident investigation reports regarding pilotage operations events that occurred between 1995 and 2015 have been reviewed.To validate the identified humanrelated risk factors(HCFs)and explore other contributory factors,survey questionnaires and semi-structured interviews with domain experts have been conducted.A structural hierarchy diagram for the identified risk factors(HCFs)has been developed and validated through experienced experts belonging to the maritime sector.A questionnaire for pair-wise comparison is carried out and analysed using the analytic hierarchy process(AHP)approach to evaluate the weight and rank the importance of the identified human causal factors.The findings of this study will benefit the maritime industry,by identifying a new database on causal factors that are contributing to the occurrence of maritime pilotage disasters.The database can be used as a stand-alone reference or help implement effective risk reduction strategies to reduce the human error,that might occur during pilotage operations. | Hassan Oraith Eddie Blanco‑Davis Zaili Yang Ben Matellini | 2021 | Journal of Marine Science and Application2021，20，3： | 2 |
| 16 | 结合风险矩阵和故障树分析法的海洋地震测量船运营的规范化安全评估显示文摘In maritime safety research,risk is assessed usually within the framework of formal safety assessment(FSA),which provides a formal and systematic methodology to improve the safety of lives,assets,and the environment.A bespoke application of FSA to mitigate accidents in marine seismic surveying is put forward in this paper,with the aim of improving the safety of seismic vessel operations,within the context of developing an economically viable strategy.The work herein takes a close look at the hazards in North Sea offshore seismic surveying,in order to identify critical risk factors,leading to marine seismic survey accidents.The risk factors leading to undesirable events are analysed both qualitatively and quantitatively.A risk matrix is introduced to screen the identified undesirable events.Further to the screening,Fault Tree Analysis(FTA)is presented to investigate and analyse the most critical risks of seismic survey operation,taking into account the lack of historical data.The obtained results show that man overboard(MOB)event is a major risk factor in marine seismic survey operation;lack of training on safe work practice,slippery deck as a result of rain,snow or water splash,sea state affecting human judgement,and poor communication are identified as the critical risk contributors to the MOB event.Consequently,the risk control options are focused on the critical risk contributors for decision-making.Lastly,suggestions for the introduction and development of the FSA methodology are highlighted for safer marine and offshore operations in general. | Gregory Asuelimen Eduardo Blanco-Davis Jin Wang Zaili Yang Dante Benjamin Matellini | 2020 | Journal of Marine Science and Application2020，19，2： | 2 |
| 17 | Global shape reconstruction of nano grid with singly fixed camera显示文摘Depth from defocus(DFD),as a typical shape reconstruction method,has been widely researched in most recent years.However,all the existing DFD algorithms require at least two defocused images with different camera parameters.Unfortunately,in micro/nano manipulation,any change on visual sensor's parameters is absolutely forbidden.Therefore,a novel DFD method to reconstruct the shape of a nano grid on micro/nano scale is researched in this paper.First,the blurring imaging model is constructed with the relative blurring and the diffusion equation.Second,the relationship between depth and blurring is discussed from four aspects.Subsequently,depth measurement problem is transformed into an optimization issue which is solved using the gradient flow algorithm.Finally,experiment results and error analysis are conducted to show the feasibility and effectiveness of the proposed method. | WEI YangJie WU ChengDong DONG ZaiLi | 2011 | Science China(Technological Sciences)2011，54，4： | 2 |
| 18 | Di-electrophoresis assembly and fabrication of SWCNT field-effect transistor显示文摘In the process of fabricating nano electrical device or system based on single-walled carbon nanotube (SWCNT), the controllable assembly and fabrication of SWCNT field-effect transistor (SWCNT FET) is a key issue. SWCNT FET is the most basic and important component in nano electronics. After microelectrode chip of back-gate FET is designed and fabricated, di-electrophoresis technology is adopted to realize the controllable alignment and assembly of SWCNTs, based on dispersing SWCNT by sodium dodecyl sulphate (SDS) facilitated ultra-sonication technique and removing impurities by centrifugal technique. The experiments of SWCNTs assembly demonstrate that SWCNTs are aligned and assembled uniformly at the microelectrodes gap with the alignment density nearly proportional to di-electrophoresis duration and solution concentration. After the processes of rinsing, drying and improving, metallic SWCNTs among the assembled SWCNTs are burned out and residual SDS is removed, and perfect field-effect performance of SWCNT FET is eventually obtained. | TIAN XiaoJun WANG YueChao YU HaiBo DONG ZaiLi XI Ning TUNG Steve | 2009 | Chinese Science Bulletin2009，54，23： | 2 |
| 19 | An accident data-based approach for congestion risk assessment of inland waterways: a Yangtze River case显示文摘 | ZHANG Di YAN Xinping YANG Zaili | 2014 | Proceedings of the in- stitution of mechanical engineers part O : journal of risk and reliability2014，228，2： | 1 |
| 20 | Query processing in multi - user scenario for wireless sensor networks 显示文摘 | Liu Xingcheng Ma Zaili | 2011 | IEEE Sensors Journal2011，11，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员