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| 1 | asy and asyip:A new type of apoptosis-inducing gene显示文摘Apoptosis,,a wide physiological process in multicel-lular organisms, is critical for the organ development, the cell senescence, the tissue homeostasis and the resistance to infection of virus and bacteria. Apoptosis plays a key role in the regulation of the growth cell number such as the tissue construction, the elimination of abnormal or dangerous cells. Therefore, apoptosis is a stringent and effective cell quality controlling system, which reduces the number of harmful cells to the maximum, such as auto-immunity cell, cells infected by virus, tumor cells by cell suicide111. Apoptosis can be initiated by a wide variety of the extracellular and intracellular stimuli, including the developmental signals, the cellular stress and the disruption of cell cycle, transduced and amplified by the second messengers, and finally finished by the activating death effector protease. This process can be called apoptosis signal network. The defective in control of the apoptotic pathways may contribute to a variety | Bing Qi Yipeng Qi Qinzheng Liu Xiaoling Qu | 2001 | Chinese Science Bulletin2001，46，17： | 3 |
| 2 | SRSF1 plays a critical role in invariant natural killer T cell development and function显示文摘Invariant natural killer T(iNKT)cells are highly conserved innate-like T lymphocytes that originate from CD4^(+)CD8^(+)double-positive(DP)thymocytes.Here,we report that serine/arginine splicing factor 1(SRSF1)intrinsically regulates iNKT cell development by directly targeting Myb and balancing the abundance of short and long isoforms.Conditional ablation of SRSF1 in DP cells led to a substantially diminished iNKT cell pool due to defects in proliferation,survival,and TCRαrearrangement.The transition from stage 0 to stage 1 of iNKT cells was substantially blocked,and the iNKT2 subset was notably diminished in SRSF1-deficient mice.SRSF1 deficiency resulted in aberrant expression of a series of regulators that are tightly correlated with iNKT cell development and iNKT2 differentiation,including Myb,PLZF,Gata3,ICOS,and CD5.In particular,we found that SRSF1 directly binds and regulates pre-mRNA alternative splicing of Myb and that the expression of the short isoform of Myb is substantially reduced in SRSF1-deficient DP and iNKT cells.Strikingly,ectopic expression of the Myb short isoform partially rectified the defects caused by ablation of SRSF1.Furthermore,we confirmed that the SRSF1-deficient mice exhibited resistance to acute liver injury uponα-GalCer and Con A induction.Our findings thus uncovered a previously unknown role of SRSF1 as an essential post-transcriptional regulator in iNKT cell development and functional differentiation,providing new clinical insights into iNKT-correlated disease. | Jingjing Liu Menghao You Yingpeng Yao Ce Ji Zhao Wang Fang Wang Di Wang Zhihong Qi Guotao Yu Zhen Sun Wenhui Guo Juanjuan Liu Shumin Li Yipeng Jin Tianyan Zhao Hai-Hui Xue Yuanchao Xue Shuyang Yu | 2021 | Cellular & Molecular Immunology2021，18，11： | 3 |
| 3 | Characterization of a region involved in binding of measles virus H protein and its receptor SLAM (CD150)显示文摘 | Chunling Hu Peng Zhang Xin Liu Yipeng Qi Tingting Zou Qin Xu | 2004 | Biochemical and Biophysical Research Communications2004，，3： | 2 |
| 4 | Influence of coronary bifurcation angle on atherosclerosis显示文摘Hemodynamics plays a crucial role in the development and progression of coronary atherosclerosis,which is prone to occur in branch bifurcation.A n individual aortic-coronary artery model and three changed bifurcation angle models are constructed by Mimics and Freeform based on computed tomography angiography.The influence of different coronary bifurcation angles between left main(LM),left anterior descending(LAD),and left circumflex(LCX)on the blood flow field and related hemodynamic parameters are studied.It is shown that a wider bifurcation angle between LAD and LCX can cause a wider low-wall shear stress area,leading to atherosclerosis.Similarly,a decreased angle between LM and LAD is predisposed to prevent atherosclerosis.The results help to better understand the hemodynamic causes of atherosclerosis with various bifurcation angles in coronary arteries and to provide guidance for clinical assessment and prevention. | Zhaomiao Liu Shengwei Zhao Yunjie Li Feng Shen Yipeng Qi Qi Wang | 2019 | Acta Mechanica Sinica2019，35，6： | 2 |
| 5 | Overexpression of Pygopus2 protects HeLa cells from vinblastine-induced apoptosis显示文摘 | Degejing De Apeng Chen Zhiqiang Wu Songya Lv Guoqing He Yipeng Qi | 2009 | Biological Chemistry2009，，2： | 2 |
| 6 | Evaluation of menopausal status among breast cancer patients with chemotherapy-induced amenorrhea显示文摘Objective: In patients with chemotherapy-induced amenorrhea(CIA), the menopausal status is ambiguous and difficult to evaluate. This study aimed to establish a discriminative model to predict and classify the menopausal status of breast cancer patients with CIA.Methods: This is a single center hospital-based study from 2013 to 2016. The menopausal age distribution and accumulated incidence rate of CIA are described. Multivariate models were adjusted for established and potential confounding factors including age, serum concentration of estradiol(E2) and follicle-stimulating hormone(FSH),feeding, pregnancy, parity, abortions, and body mass index(BMI). The odds ratio(OR) and 95% confidence interval(95% CI) of different risk factors were estimated.Results: A total of 1,796 breast cancer patients were included in this study, among whom, 1,175(65.42%) were premenopausal patients and 621(34.58%) were post-menopause patients. Five hundred and fifty patients were included in CIA analysis, and a cumulative CIA rate of 81.64% was found in them. Age(OR: 1.856, 95% CI:1.732-1.990), serum concentration of E2(OR: 0.976, 95% CI: 0.972-0.980) and FSH(OR: 1.060, 95% CI:1.053-1.066), and menarche age(OR: 1.074, 95% CI: 1.009-1.144) were found to be associated with the patients' menopausal status. According to multivariate analysis, the discriminative model to predict the menopausal status is Logit(P)=-28.396+0.536 Age-0.014 E2+0.031 FSH. The sensitivities for this model were higher than 85%, and its specificities were higher than 89%.Conclusions: The discriminative model obtained from this study for predicting menstrual state is important for premenopausal patients with CIA. This model has high specificity and sensitivity and should be prudently used. | Bailin Zhang Jinqi Wu Rongshou Zheng Qian Zhang Margaret Zhuoer Wang Jun Qi Haijing Liu Yipeng Wang Yang Guo Feng Chen Jing Wang Wenyue Lyu Jidong Gao Yi Fang Wanqing Chen Xiang Wang | 2018 | Chinese Journal of Cancer Research2018，30，4： | 2 |
| 7 | High efficiency fluorescent white organic light-emitting diodes with red, green and blue separately monochromatic emission layers显示文摘 | Zhiqiang Zhang Qi Wang Yanfeng Dai Yipeng Liu Lixiang Wang Dongge Ma | 2009 | Organic Electronics2009，，3： | 1 |
| 8 | Efects on the Early Development of the Pearl Sac by Cytochrome c,Yolk Lecithin and Polyvinyplyrro显示文摘EfectsontheEarlyDevelopmentofthePearlSacbyCytochromec,YolkLecithinandPolyvinyplyroZhengJunying,ZhangMuxian,LiuTingColegeofLif... | Jin Hailing Qi Yipeng Jin Tianquan Mallam Nockjoshua Huang Yongxiu | 1997 | Wuhan University Journal of Natural Sciences1997，2，2： | 1 |
| 9 | Preparation of chitosan-copper complexes and their antitumor activity 显示文摘 | Yong Zheng Ying Yi Yipeng Qi | 2006 | Bioorganic & Medicinal Chemistry Letters2006，16，15： | 1 |
| 10 | Cloning and Expression of the vp39 Gene of Bombyx mori Nuclear Polyhedrosis Virus in E.coli显示文摘The nuclear capsid protein gene (vp39) or Bombyx mori nuclear polykedrosis virus (BmNPV)was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5’ and 3’ terminal area orthe amplirled vp39 gene were sequenced with sliver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed into E. coli BL21. This gene was highly expressed byIPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressedamount reached maxlum in 4 h with IPTG induction. | Liu Dell, Sun Xiaojie, Qi Yipeng, Zhu Ying, Jin Tianquan(Institute of Virology, Wuhan University,Wuhan 430072, China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，1： | 1 |
| 11 | High efficiency fluorescent white organic light-emitting diodes with red, green and blue separately monochromatic emission layers显示文摘 | Zhiqiang Zhang Qi Wang Yanfeng Dai Yipeng Liu Lixiang Wang Dongge Ma | 2009 | Organic Electronics2009，，3： | 1 |
| 12 | Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5显示文摘 | Shumin Zhou Wei Xiao Qingwen Wan Changhua Yi Feifei Xiao Yingle Liu Yipeng Qi | 2010 | Biochemical and Biophysical Research Communications2010，，2： | 1 |
| 13 | Structure and function of bak gene and its apoptosis-inducing mechanism显示文摘The Bcl-2 family plays an important role in the regulation of apoptosis. Some members in the family prevent apoptosis while others promote it. The newly discovered bcl-2 homolog,bak, promotes the apoptosis induced by a variety of stimuli. It can interact with the anti-apoptotic members through its BH3 domain and antagonize their activity. Investigation results suggest that the expression of bak gene becomes altered in some diseases, such as cancer. The preferential stimulation of Bak-induced apoptosis, therefore, may be involved in the mechanisms of some antitumor drugs. | Yan Li Yipeng Qi Gengfu Xiao | 1999 | Chinese Science Bulletin1999，44，7： | 1 |
| 14 | Analysis of proteins that interact with nucleocapsid protein of SARS-CoV using 15-mer phage-displayed library显示文摘Analysis of proteins that interact with N protein of SARS-CoV using 15-mer phage-displayed library will help to explore the virus pathogenesis and to develop new drugs and vaccines against SARS. In this study,we cloned,expressed and purified N protein of SARS-CoV. This 46-kD N protein was verified by SDS-PAGE and Western-blot. Then,the peptides binding-specific to N protein were identified using 15-mer phage-displayed library. Surprisingly,all of the 89 clones from monoclonal ELISA were positive (S/N>2.1) and the result was further confirmed experimentally once again. Six N protein-binding pep-tides,designated separately as SNA1,SNA2,SNA4,SNA5,SNA9 and SNG11,were selected for se-quencing. Sequence analysis suggested that SNA5 shared approximatively 100% sequence identity to SNA4,SNA2,SNA9 and SNA1. In addition,the binding specificity of the 15-mer peptides with the SARS-CoV N protein was further demonstrated by blocking ELISA using the synthetical 15-mer peptide according to the deduced amino acid sequence of SNA5. Also,the deduced amino sequence of SNA5 was compared with proteins in translated database using the tblastx program,and the results showed that the proteins with the highest homology were Ubiquinol-cytochrome c reductase iron-sulfur sub-units (UCRI or UQCR),otherwise known as the Rieske iron-sulfur proteins (RISP). Notablely,in the 2Fe-2S redox centre of UCRI,there were 6 residues GGW(Y)F(Y)CP compatible to the residues (po-sition 2→7,GGWFCP7) of the NH2-terminal of the 15-mer peptide,which indicated higher binding specificity between the N protein of SARS-CoV and the redox centre of UCRI to some extent. Here,the possible molecular mechanisms of SARS-CoV N protein in the pathogenesis of SARS are discussed. | LIU ZhengXue WANG ZhanHui LIU YingLe DONG Wei QI YiPeng | 2007 | Chinese Science Bulletin2007，52，15： | 1 |
| 15 | Cloning and identification of measles virus receptor gene from marmoset cells显示文摘The measles virus (MV) strains with mutated hemagglutinin gene (ha) lost the capacity to infect its sensitive host cells (Vero cells), but it may infect the marmoset B-lymphoblastoid cell line B95a. From above, we can presume that there is a novel cellular receptor for those measles virus strains on B95a cells. Using the yeast two-hybrid system, we screened and cloned a novel gene-bip (B-lymphoblastoid interaction protein of marmoset) from B95a cell cDNA library, which encoded a protein interacting with measles virus hemagglutinin protein (Ha). The bip cDNA was 1540 base pairs in length and contained a unique open reading frame (ORF) of 1011 base pairs encoding a transmembrane protein of 337 amino acid residues. The primary structure of amino acids residue is predicted that the Bip comprised a hydrophobic transmembrane domain and a hydrophobic leader region. The researches about the deletion mutants showed that the deletion of transmembrane domain in Bip did not affect the interaction between Bip and | Lingyun Li Xin Liu Peng Zhang Yipeng Qi Ming Cheng | 2002 | Chinese Science Bulletin2002，47，20： | 1 |
| 16 | Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008显示文摘 | Yipeng Sun Juan Pu Zhanlei Jiang Tao Guan Yingju Xia Qi Xu Linqing Liu Bo Ma Fulin Tian E.G. Brown Jinhua Liu | 2010 | Veterinary Microbiology2010，，3： | 1 |
| 17 | Expression of Green Fluorescent Protein Gene with Baculovirus Vectorin Insect Cells显示文摘ExpresionofGreenFluorescentProteinGenewithBaculovirusVectorinInsectCelsHuJianhong,ZhuFanxiu,QiYipeng,HuangYongxiuInstituteof... | Hu Jianhong Zhu Fanxiu Qi Yipeng Huang Yongxiu | 1997 | Wuhan University Journal of Natural Sciences1997，2，1： | 0 |
| 18 | Construction of transposon-mediated baculovirus vector and expression of green fluorescent protein in insect cells and larvae显示文摘A transposon-shuttle vector Hanpvid was constructed by using wild-type genomic DNA from Heliothis armigera nuclear polyhedrosis virus (HaNPV). lt could replicate in E. coli cells as a large plasmid and remain infectious when being induced into insect cells.Hanpvid comprises HaNPV DNA and a transposon cassette which includes a miniF replicon, a kanamycin resistance gene (kan), lacZa and an attachment site for Tn7 (attTn7). Recombinant virus rHa-FaGP was obtained after transposition of a donor plasmid carrying green fluorescent protein gene (gfp) and polyhedrin gene (ocu) into attTn7.SDS-PAGE analysis shows that both gfp and ocu genes were highly expressed in Heliothis armigera cells. Green Hemolymphocytes can be seen under a fluorescent microscope 4 d after recombinant virus rHa-FaGP infected the third-instar larvae. The infected larvae show strong green fluorescence 6 d post infection. | Ying Zhu Yipeng Qi | 1999 | Chinese Science Bulletin1999，44，2： | 0 |
| 19 | Measles Virus(Nepal Strain)Hemagglutinin Gene: Cloning,Complete Nucleotide Sequence Analysis and Expression in COS Cells显示文摘0IntroductionMeaslesvirus(MV)causesahighlyconta-giousdiseasewhosepathogenicrateisalmost100%withinsensitivegroups.Onrareoccasi... | Li, Lingyun Qi, Yipeng | 1998 | Wuhan University Journal of Natural Sciences1998，3，3： | 0 |
| 20 | Identification of Caspase-cleavage site in the apoptosis suppressor protein P49 from the Spodoptera littoralis nucleopolyhedrovirus显示文摘The p49 gene from baculovirus Spodoptera lit-toralis nucleopolyhedrovirus (SlNPV) was able to suppress apoptosis of Sf9 cells induced by virus infection. Ectopically expressed P49 protein had the capacity to inhibit the activity of Caspases, being the executioner of apoptosis. Digestion of P49 with human Caspase or Bm-Caspase both yielded 10 and 40 kD fragments. Checking the sequence of P49, we found that the motif 91-TVTDG-95 of P49 was the sequence recog-nized by Caspases. The mutant of P49, Asp94Ala, could not be cut by both caspases and lost its caspases inhibition. Meanwhile, Thr91Ala mutant permitted the cleavage and partially retained its activity of caspases inhibition. We also found that P49 was a substrate of upstream initiator caspase and downstream effector Caspases, indicating that P49 was a broad specificity Caspase inhibitor. | PEI Zifei, LIU Yingle, LIU Qingzhen & QI Yipeng Institute of Virology, Wuhan University, Wuhan 430072, China Correspondence should be addressed to Qi Yipeng (e-mail: qiyipeng@ whu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，8： | 0 |
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