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| 1 | A New Critical Nitrogen Dilution Curve for Rice Nitrogen Status Diagnosis in Northeast China显示文摘In-season diagnosis of crop nitrogen(N) status is crucial for precision N management. Critical N(N_c) dilution curve and N nutrition index(NNI) have been proposed as effective methods to diagnose N status of different crops. The N_c dilution curves have been developed for indica rice in the tropical and temperate zones and japonica rice in the subtropical-temperate zone, but they have not been evaluated for short-season japonica rice in Northeast China. The objectives of this study were to evaluate the previously developed N_c dilution curves for rice in Northeast China and to develop a more suitable N_c dilution curve in this region. A total of17 N rate experiments were conducted in Sanjiang Plain, Heilongjiang Province in Northeast China from 2008 to 2013. The results indicated that none of the two previously developed N_c dilution curves was suitable to diagnose N status of the short-season japonica rice in Northeast China. A new N_c dilution curve was developed and can be described by the equation N_c = 27.7 W^(-0.34) if W ≥ 1 Mg dry matter(DM) ha^(-1) or N_c = 27.7 g kg^(-1) DM if W < 1 Mg DM ha^(-1), where W is the aboveground biomass. This new curve was lower than the previous curves. It was validated using a separate dataset, and it could discriminate non-N-limiting and N-limiting nutritional conditions. Additional studies are needed to further evaluate it for diagnosing N status of different rice cultivars in Northeast China and develop efficient non-destructive methods to estimate NNI for practical applications. | HUANG Shanyu MIAO Yuxin CAO Qiang YAO Yinkun ZHAO Guangming YU Weifeng SHEN Jianning YU Kang Georg BARETH | 2018 | Pedosphere2018，28，5： | 10 |
| 2 | Comparison of alternative extraction methods for secretome profiling in human hepatocellular carcinoma cells显示文摘Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma. | CAO Jing SHEN ChengPin ZHANG Jun YAO Jun SHEN HuaLi LIU YinKun LU HaoJie YANG PengYuan | 2011 | Science China(Life Sciences)2011，54，1： | 8 |
| 3 | Protein expression and fucosylated glycans of the serum haptoglobin-β subunit in hepatitis B virus-based liver diseases显示文摘Glycosylation,调整 glycoproteins 的配置和功能,是最重要的 translational 以后修正。学习是在 glycan 和浆液 haptoglobin- 子单元(Hp-) 的蛋白质部分观察微分模式的这的目的与肝炎 B 从病人净化了病毒(HBV ) 感染,肝肝硬化(LC ) ,或 hepatocellular 癌(HCC ) 。2-D 胶化电气泳动和多路的 proteomics 染色技术被采用调查 Hp- glycan 水平是否与蛋白质水平成正比。Multi-lectin 污点,高效的液体层析(HPLC ) ,和西方的污点分析被执行识别 Hp- 份量上的 glycoform。我们的实验证明到 Hp- 的 glycosylated 形式的全部的浆液 Hp- 的比率与不同的肝疾病在病人之中变化了。全部的 Hp- 蛋白质表示水平比 LC 在 HCC 是高得多的,当 fucosylated Hp- 的一个增长比例也在 HBV 和健康控制与那相比在 LC 和 HCC 病人被观察时。微分 fucosylation 被 HPLC 和反人的 SialylLewis X 抗体进一步作为路易斯十世结构识别。在结论, Hp- glycan 和全部的蛋白质表示的异常引申可以是为早 hepatocarcinogenesis 的有希望的 biomarker。 | Hong Shu Shu Zhang Xiaonan Kang Shan Li Xue Qin Chun Sun Haojie Lu Yinkun Liu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，7： | 6 |
| 4 | Serum paraoxonase 1 heteroplasmon, a fucosylated, and sialylated glycoprotein in distinguishing early hepatocellular carcinoma from liver cirrhosis patients显示文摘浆液 glycoproteins 的异常 glycan 结构在 hepatocellular 癌(HCC ) 的不同阶段创造唯一的模式,它为 HCC 的早诊断提供潜在的 glycan biomarkers。在这研究,双人脚踏车 lectin 亲密关系层析使用 aleuria aurantia lectin ( AAL )和小麦细菌凝集素( WGA )被处理从 27 肝肝硬化( LC )和 27 个早 HCC 病人净化 fucosylated 和 sialylated 浆液 glycoproteins ,在哪个 122 glycoproteins 被液体层析双人脚踏车团 spectrometry ( LC-MSMS )最后外面屏蔽。在 LC-MSMS 识别的 122 蛋白质之中,他们中的 8 个仅仅在 HCC 浆液被识别, 6 仅仅在 LC 浆液存在。浆液 paraoxonase (PON1 ) 1 是从 47 个单个病人的 immunoprecipitated 并且在 LC 比那些在 HCC 浆液由 lectins,出现提高的 fucosylation 和 sialylation 弄污浆液。在为 AAL 反应的 PON1 的 ROC 曲线(AUROC ) 下面的区域在把早 HCC 区分开来与 LC 与 71.4% 的敏感和 94.7% 的特性是 0.892。同样, WGA 反应的 PON1 与 95.2% 的敏感和 78.9% 的特性有 0.902 的 AUROC。数据显示浆液 PON1 的 glycan 差别可能为把早 HCC 与 LC 病人区分开来提供同样潜在的 glycan biomarkers。 | Chun Sun Pei Chen Qiaopei Chen Lu Sun Xiaonan Kang Xue Qin Yinkun Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，9： | 6 |
| 5 | Comparison of thermal and light performance in two typical Chinese solar greenhouses in Beijing显示文摘Solar greenhouses have been used for producing vegetables in northern China during early spring,late autumn or over-winter.To improve the thermal performance of solar greenhouses,a traditional type and a retrofitted design were comparatively evaluated.In the retrofitted design,three adjustments were incorporated:the material and structure of the walls,south-facing roof angle,and structure of the north-facing back-roof.The results indicated that the thermal and light performance of the retrofitted greenhouse was much better than that of the traditional greenhouse.Specifically,the daily mean temperature,minimum air temperature,and soil temperature inside the greenhouses after retrofit ting were increased by 1.3,2.4,and 1.9℃,respectively,meanwhile,the daily total solar radiation and PAR were increased by 28.2%and 9.2%,respectively.The wall temperature and its daily variation range were reduced with increasing depth and height.The characteristic analysis of heat storage and release indicated that higher locations have longer heat storage,and shorter heat release time in vertical direction,as well as a lower ratio of heat release to storage.In horizontal direction,the western wall has the shortest heat storage time but the highest heat release flux density.Altogether,the heat storage time of the wall is 1.5 h less than that of the soil.The heat storage flux density of the wall is 1.5 times of that of the soil,but the heat release flux is only 61%of the soil’s value.The total wall heat storage is half of that of the soil in the greenhouse;the total wall heat release amount is only a quarter of that of the soil.Therefore,the thermal environment of solar greenhouses can be further improved by improving the thermal insulation properties of the wall. | Fan Xu Chao Shang Hongling Li Xuzhang Xue Weituo Sun Hong Chen Yinkun Li Zhenhe Zhang Xinxu Li Wenzhong Guo | 2019 | International Journal of Agricultural and Biological Engineering2019，12，1： | 4 |
| 6 | Quantitative analysis of site-specific N-glycans on sera haptoglobin βchain in liver diseases显示文摘在有临床的申请的潜力的 glycoproteins 的 N-glycans 的地点特定的描述是重要的。在我们的以前的报告,链被发现在肝疾病不同的 sera haptoglobin (Hp ) 的全面 N-glycans。Hp 链包含 N-glycosylation 的四个潜在的地点。在这研究,我们以一种地点特定的方式在 Hp 链上调查了 N-glycans 的潜在的变化。在象有肝炎 B 病毒( HBV )的病人一样的健康个人的 Sera Hp 链,肝肝硬化( LC )和 hepatocellular 癌( HCC )被净化,消化并且使遭到了到液体 chromatography-electrospray 电离高度精力碰撞分离团 spectrometry ,它允许鉴定和 glycopeptide 的结构决心,象在每 glycopeptide 上在场的 glycans 的相对 quantification 一样。量的结果表明 NLFLN207HSEN211ATAK 和 fucosylated 的 sialylation 根本组织在 HBV 病人和健康个人与那些相比在 LC 和 HCC 病人显著地增加的 glycopeptides。在各种各样的肝疾病的 Hp 链的一套不同 N-glycan 模式被决定了。因此, sialylated 和 Hp 链力量的 fucosylated glycoforms 与早 hepatocarcinogenesis 有关并且可能也作为为 LC 和 HCC 病人的新奇微分标记有用。 | Shu Zhang Kai Jiang Chun Sun Haojie Lu Yinkun Liu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 3 |
| 7 | The Significance of MMP-9 Over MMP-2 in HCC Invasiveness and Recurrence of Hepatocellular Carcinoma After Curative Resection显示文摘 | Rongxin Chen Jiefeng Cui Changde Xu Tongchun Xue Kun Guo Dongmei Gao Yinkun Liu Shenglong Ye Zhenggang Ren | 2012 | Annals of Surgical Oncology2012，，3： | 2 |
| 8 | Prevalence and risk factors of hypertension based on repeated measurements in Chinese children and adolescents显示文摘 | Linghui Meng Yajun Liang Junting Liu Yuehua Hu Yinkun Yan Jie Mi | 2013 | Blood Pressure2013，，1： | 1 |
| 9 | GENOME EDITING:A GROUND BREAKING RESEARCH HAS BEEN RANKED TOP 10 ENGINEERING FRONTS FROM 2017 TO 2021显示文摘‘Global Engineering Fronts’ is a report released by the Chinese Academy of Engineering(CAE) every year since 2017, which aims to assemble talents in the field of engineering science and technology to represent the global engineering research and development fronts by reviewing global papers, patents, and other data. The results are also expected to provide a reference for people on responding to global challenges and achieving sustainable development. | Jianxiang XU Yunzhou LI Yinkun YAO Jie ZHAO Jingyue TANG Zixuan FENG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，2： | 1 |
| 10 | Active canopy sensor-based precision N management strategy for rice显示文摘 | Yinkun Yao Yuxin Miao Shanyu Huang Lei Gao Xiaobo Ma Guangming Zhao Rongfeng Jiang Xinping Chen Fusuo Zhang Kang Yu Martin Gnyp Georg Bareth Cheng Liu Liqin Zhao Wen Yang Huamin Zhu | 2012 | Agronomy for Sustainable Development2012，，4： | 1 |
| 11 | Abundance and distribution of meiofauna in the Chukchi Sea显示文摘The metazoan meiofauna in the Chukchi Sea were collected from seven shallow water stations(depths ranging 46 to 52 m) and five deep sea stations(depths ranging between 393 and 2 300 m) during the 4th Chinese National Arctic Research Expedition in 2010. The results showed that abundance of meiofauna was higher in shallow water sediments(average of 2 445 ind./(10 cm2)) than in deep sea sediments(407.06 ind./(10 cm2)). A UNIANOVA test for difference between the two different regions was highly significant(F=101.15, p<0.01). Nematodes were numerically dominant, representing(96.6±4.6)% of the total meiofaunal abundance at the shallow water stations and(98.90±1.42)% at deep sea stations. The number of higher taxonomic groups and abundance of meiofauna were higher at Stas CC1, CC4, and R06 near the Bering Strait and the continent, than at the rest of the shallow water and deep sea stations. The primary factors causing the differences were concentrations of nutrients P and Si of bottom seawater(R=0.831, p<0.003), followed by depth(R=-0.655, p<0.05) and sand fractions of sediments(R=0.632, p <0.05). The numbers of meiofauna on the 65 μm and 32 μm sieves were significantly higher than those on the rest of the screens. Differences in numbers of meiofauna retained on screens with different mesh openings were highly significant among all sampling stations(F=31.60, p<0.01). The highest numbers of individuals on screens with 32 μm mesh openings were found at deep sea stations. The number of meiofauna in the top 0–1, 1–2, and 2–4 cm segments constituted 84.4% of the total and was significantly higher than those in the bottom 4–6 and 6–10 cm segments(F=15, p<0.01). | LIN Rongcheng HUANG Dingyong GUO Yuqing CHANG Yu CAO Yinkun WANG Jianjia | 2014 | Acta Oceanologica Sinica2014，33，6： | 1 |
| 12 | Heat-shock protein 27 : a potential biomarker for hepatocellalar carcinoma identified by serum proteome analysis 显示文摘 | FENG Jutao LIUu Yinkun SONG Haiyan | 2005 | Proteomics2005，5，17： | 1 |
| 13 | Differentialpro-teomic analysis ofhuman hepatocellular carcinoma celllinemetastasis-associ-ated proteins显示文摘 | Cui Jiefeng Liu Yinkun Pan Boshen | 2002 | J Cancer Res Clin Oncol2002，130，10： | 1 |
| 14 | Hyperspectral canopy sensing of paddy rice aboveground biomass at different growth stages显示文摘 | Martin L. Gnyp Yuxin Miao Fei Yuan Susan L. Ustin Kang Yu Yinkun Yao Shanyu Huang Georg Bareth | 2014 | Field Crops Research2014，，： | 1 |
| 15 | Tumor-derived secretory clusterin induces epithelial–mesenchymal transition and facilitates hepatocellular carcinoma metastasis显示文摘 | Cun Wang Kai Jiang Xiaonan Kang Dongmei Gao Chun Sun Yan Li Lu Sun Shu Zhang Xiaohui Liu Weizhong Wu Pengyuan Yang Kun Guo Yinkun Liu | 2012 | International Journal of Biochemistry and Cell Biology2012，，12： | 1 |
| 16 | Noncommunicable chronic disease prevention should start from childhood显示文摘Noncommunicable chronic diseases(NCDs),such as cardiovascular disease(CVD),diabetes,cancers,and osteoporosis,are the leading causes of death and health costs.According to the 2020 Report on Chinese Resident’s Chronic Disease and Nutrition,NCDs account for 88.5%of China’s disease burden in 2019,more than all other causes combined. | Yinkun Yan Jie Mi | 2021 | Pediatric Investigation2021，5，1： | 1 |
| 17 | Mutation and overexpression of the β-catenin gene may play an important role in primary hepatocellular carcinoma among Chinese people显示文摘 | Jian Cui Xinda Zhou Yinkun Liu Zhaoyou Tang | 2001 | Journal of Cancer Research and Clinical Oncology2001，，9： | 1 |
| 18 | Prediction of chronic hepatitis B, liver cirrhosis and hepatocellular carcinoma by SELDI-based serum decision tree classification显示文摘 | Jiefeng Cui Xiaonan Kang Zhi Dai Cheng Huang Haijun Zhou Kun Guo Yan Li Yu Zhang Ruixia Sun Jie Chen Yang Li Zhaoyou Tang Toshimasa Uemura Yinkun Liu | 2007 | Journal of Cancer Research and Clinical Oncology2007，，11： | 1 |
| 19 | Computational fluid dynamics simulations and redesign of a screw conveyor reactor 显示文摘 | Yinkun Wan Thomas R Hanley | 2004 | Applied Biochemistry and Biotechnology2004，，： | 1 |
| 20 | Engineering Saccharomyces cerevisiae for enhanced(–)-α-bisabolol production显示文摘(–)-α-Bisabolol is naturally occurring in many plants and has great potential in health products and pharma-ceuticals.However,the current extraction method from natural plants is unsustainable and cannot fulfil the increasing requirement.This study aimed to develop a sustainable strategy to enhance the biosynthesis of(–)-α-bisabolol by metabolic engineering.By introducing the heterologous gene MrBBS and weakening the competitive pathway gene ERG9,a de novo(–)-α-bisabolol biosynthesis strain was constructed that could produce 221.96 mg/L(–)-α-bisabolol.Two key genes for(–)-α-bisabolol biosynthesis,ERG20 and MrBBS,were fused by a flexible linker(GGGS)3 under the GAL7 promoter control,and the titer was increased by 2.9-fold.Optimization of the mevalonic acid pathway and multi-copy integration further increased(–)-α-bisabolol production.To promote product efflux,overexpression of PDR15 led to an increase in extracellular production.Combined with the optimal strategy,(–)-α-bisabolol production in a 5 L bioreactor reached 7.02 g/L,which is the highest titer reported in yeast to date.This work provides a reference for the efficient production of(–)-α-bisabolol in yeast. | Yinkun Jiang Lu Xia Song Gao Ning Li Shiqin Yu Jingwen Zhou | 2023 | Synthetic and Systems Biotechnology2023，8，2： | 0 |
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