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| 1 | 3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration显示文摘Hydrogel scaffolds are attractive for tissue defect repair and reorganization because of their human tissue-like characteristics.However,most hydrogels offer limited cell growth and tissue formation ability due to their submicron-or nano-sized gel networks,which restrict the supply of oxygen,nutrients and inhibit the proliferation and differentiation of encapsulated cells.In recent years,3D printed hydrogels have shown great potential to overcome this problem by introducing macro-pores within scaffolds.In this study,we fabricated a macroporous hydrogel scaffold through horseradish peroxidase(HRP)-mediated crosslinking of silk fibroin(SF)and tyramine-substituted gelatin(GT)by extrusion-based low-temperature 3D printing.Through physicochemical characterization,we found that this hydrogel has excellent structural stability,suitable mechanical properties,and an adjustable degradation rate,thus satisfying the requirements for cartilage reconstruction.Cell suspension and aggregate seeding methods were developed to assess the inoculation efficiency of the hydrogel.Moreover,the chondrogenic differentiation of stem cells was explored.Stem cells in the hydrogel differentiated into hyaline cartilage when the cell aggregate seeding method was used and into fibrocartilage when the cell suspension was used.Finally,the effect of the hydrogel and stem cells were investigated in a rabbit cartilage defect model.After implantation for 12 and 16 weeks,histological evaluation of the sections was performed.We found that the enzymatic cross-linked and methanol treatment SF5GT15 hydrogel combined with cell aggregates promoted articular cartilage regeneration.In summary,this 3D printed macroporous SF-GT hydrogel combined with stem cell aggregates possesses excellent potential for application in cartilage tissue repair and regeneration. | Qingtao Li Sheng Xu Qi Feng Qiyuan Dai Longtao Yao Yichen Zhang Huichang Gao Hua Dong Dafu Chen Xiaodong Cao | 2021 | Bioactive Materials2021，6，10： | 6 |
| 2 | New Localities and Palaeoscolecid Worms from the Cambrian(Stage 4,Series 2)Guanshan Biota in Kunming,Yunnan,South China显示文摘The Guanshan Biota from the Cambrian Stage 4 Wulongqing Formation is a typical Burgess-shale type Lagerst?tte that had witnessed the Cambrian explosion. Here we report two new localities of the Guanshan Biota,which is located at Baimei village,south of Kunming and Xinglong village,Wuding County,Yunnan Province respectively. Both localities produce soft-body fossils and several new taxa,e.g. a new species of palaeoscolecidans(Palaeoscolex xinglongensis sp. nov.) reported herein. The comparative study of the new species with similar form not only indicates that there is a diversification of palaeoscolecid species in Guanshan Biota,but also strengthens the ties between the older Chengjiang Biota and the younger Kaili Biota(and also the coeval Burgess Shale community). Three paleoecological features,including high diversity,little disparity and richness of palaeoscolecid worms,are summarized as a case study to represent the differences between Guanshan and Chengjiang Biota. | LIU Jianni HAN Jian LI Jinshu WU Yichen PENG Jie QI Nan YANG Yiyao LI Juan | 2016 | Acta Geologica Sinica(English Edition)2016，90，6： | 4 |
| 3 | Novel HDAC5-interacting motifs of Tbx3 are essential for the suppression of E-cadherin expression and for the promotion of metastasis in hepatocellular carcinoma显示文摘Tbx3,a transcriptional repressor,is essential in the organogenesis of vertebrates,stem cell self-renewal and differentiation,and the carcinogenesis of multiple tumor types.However,the mechanism by which Tbx3 participates in the metastasis of hepatocellular carcinoma(HCC)remains largely unknown.In this study,we show that Tbx3 was dramatically upregulated in clinical HCC samples and that elevated expression of Tbx3 promoted cancer progression.To determine the underlying mechanism,systematic glycine scan mutagenesis and deletion assays were performed.We identified two critical motifs,^(585)LFSYPYT^(591)and^(604)HRH^(606),that contribute to the repression of transcriptional activity.These motifs are also essential for Tbx3 to promote cell migration and metastasis both in vitro and in vivo via the suppression of E-cadherin expression.More importantly,Tbx3 directly interacts with HDAC5 via these motifs,and an HDAC inhibitor blocks Tbx3-mediated cell migration and the downregulation of E-cadherin in HCC.As Tbx3 is involved in the carcinogenesis of multiple types of human cancers,our findings suggest an important target for anticancer drug development. | Liang Dong Qi Dong Ying Chen Yichen Li Bao Zhang Fanghang Zhou Xiaoming Lyu George GChen Paul Lai Hsiang-fu Kung Ming-Liang He | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 3 |
| 4 | ZbAGL11, a class D MADS-box transcription factor of Zanthoxylum bungeanum, is involved in sporophytic apomixis显示文摘Apomixis is a reproductive model that bypasses sexual reproduction,so it does not require the combination of paternal and maternal gametes but instead results in the production of offspring directly from maternal tissues.This reproductive mode results in the same genetic material in the mother and the offspring and has signi fi cant applications in agricultural breeding.Molecular and cytological methods were used to identify the reproductive type of Zanthoxylum bungeanum(ZB).Fluorescence detection of the ampli fi ed products of 12 pairs of polymorphic SSR primers showed consistent fl uorescence signals for mother and offspring,indicating that no trait separation occurred during reproduction.In addition,the cytological observation results showed differentiation of ZB embryos(2n)from nucellar cells(2n)to form inde fi nite embryonic primordia and then form adventitious embryos(2n),indicating that the apomictic type of ZB is sporophytic apomixis.The MADS-box transcription factor ZbAGLll was highly expressed during the critical period of nucellar embryo development in ZB.Unpollinated ZbAGLll-OE Arabidopsis produced fertile offspring and exhibited an apomictic phenotype.The overexpression of ZbAGL11 increased the callus induction rate of ZB tissue.In addition,the results of the yeast two-hybrid experiment showed that ZbAGL11 could interact with the ZbCYP450 and ZbCAD11 proteins.Our results demonstrate that ZbAGL11 can cause developmental disorders of Arabidopsis fl ower organs and result in apomixis-like phenotypes. | Xitong Fei Qianqian Shi Yichen Qi Shujie Wang Yu Lei Haichao Hu Yulin Liu Tuxi Yang Anzhi Wei | 2021 | Horticulture Research2021，8，1： | 3 |
| 5 | Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants显示文摘 | CAN‐FANGNIU WEIWEI QI‐YUNZHOU AI‐GUOTIAN YU‐JUNHAO WAN‐KEZHANG BIAOMA QINGLIN ZHENG‐BINZHANG JIN‐SONGZHANG SHOU‐YICHEN | 2012 | Plant Cell & Environment2012，，6： | 1 |
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