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| 1 | 3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration显示文摘Hydrogel scaffolds are attractive for tissue defect repair and reorganization because of their human tissue-like characteristics.However,most hydrogels offer limited cell growth and tissue formation ability due to their submicron-or nano-sized gel networks,which restrict the supply of oxygen,nutrients and inhibit the proliferation and differentiation of encapsulated cells.In recent years,3D printed hydrogels have shown great potential to overcome this problem by introducing macro-pores within scaffolds.In this study,we fabricated a macroporous hydrogel scaffold through horseradish peroxidase(HRP)-mediated crosslinking of silk fibroin(SF)and tyramine-substituted gelatin(GT)by extrusion-based low-temperature 3D printing.Through physicochemical characterization,we found that this hydrogel has excellent structural stability,suitable mechanical properties,and an adjustable degradation rate,thus satisfying the requirements for cartilage reconstruction.Cell suspension and aggregate seeding methods were developed to assess the inoculation efficiency of the hydrogel.Moreover,the chondrogenic differentiation of stem cells was explored.Stem cells in the hydrogel differentiated into hyaline cartilage when the cell aggregate seeding method was used and into fibrocartilage when the cell suspension was used.Finally,the effect of the hydrogel and stem cells were investigated in a rabbit cartilage defect model.After implantation for 12 and 16 weeks,histological evaluation of the sections was performed.We found that the enzymatic cross-linked and methanol treatment SF5GT15 hydrogel combined with cell aggregates promoted articular cartilage regeneration.In summary,this 3D printed macroporous SF-GT hydrogel combined with stem cell aggregates possesses excellent potential for application in cartilage tissue repair and regeneration. | Qingtao Li Sheng Xu Qi Feng Qiyuan Dai Longtao Yao Yichen Zhang Huichang Gao Hua Dong Dafu Chen Xiaodong Cao | 2021 | Bioactive Materials2021，6，10： | 6 |
| 2 | The Msi1-mTOR pathway drives the pathogenesis of mammary and extramammary Paget’s disease显示文摘Mammary and extramammary Paget’s Diseases(PD)are a malignant skin cancer characterized by the appearance of Paget cells.Although easily diagnosed,its pathogenesis remains unknown.Here,single-cell RNA-sequencing identified distinct cellular states,novel biomarkers,and signaling pathways—including mTOR,associated with extramammary PD.Interestingly,we identified MSI1 ectopic overexpression in basal epithelial cells of human PD skin,and show that Msi1 overexpression in the epidermal basal layer of mice phenocopies human PD at histopathological,single-cell and molecular levels.Using this mouse model,we identified novel biomarkers of Paget-like cells that translated to human Paget cells.Furthermore,single-cell trajectory,RNA velocity and lineage-tracing analyses revealed a putative keratinocyte-to-Paget-like cell conversion,supporting the in situ transformation theory of disease pathogenesis.Mechanistically,the Msi1-mTOR pathway drives keratinocyte-Paget-like cell conversion,and suppression of mTOR signaling with Rapamycin significantly rescued the Paget-like phenotype in Msi1-overexpressing transgenic mice.Topical Rapamycin treatment improved extramammary PD-associated symptoms in humans,suggesting mTOR inhibition as a novel therapeutic treatment in PD. | Yongli Song Christian F.Guerrero-Juarez Zhongjian Chen Yichen Tang Xianghui Ma Cong Lv Xueyun Bi Min Deng Lina Bu Yuhua Tian Ruiqi Liu Ran Zhao Jiuzhi Xu Xiaole Sheng Sujuan Du Yeqiang Liu Yunlu Zhu Shi-jun Shan Hong-duo Chen Yiqiang Zhao Guangbiao Zhou Jianwei Shuai Fazheng Ren Lixiang Xue Zhaoxia Ying Xing Dai Christopher J.Lengner Bogi Andersen Maksim V.Plikus Qing Nie Zhengquan Yu-Show | 2020 | Cell Research2020，30，10： | 2 |
| 3 | Trastuzumab combined with docetax-el-based regimens in previously treated metastatic gastric cancerpatients with HER2 over-expression显示文摘 | Dai GH Shi YiChen L | 2012 | Hepatogastroenterology2012，59，120： | 1 |
| 4 | Maternal genetic history of ancient Tibetans over the past 4000 years显示文摘The settlement of the Tibetan Plateau epitomizes human adaptation to a high-altitude environment that poses great challenges to human activity.Here,we reconstruct a 4000-year maternal genetic history of Tibetans using 128 ancient mitochondrial genome data from 37 sites in Tibet.The phylogeny of haplotypes M9a1a,M9a1b,D4g2,G2a’c,and D4i show that ancient Tibetans share the most recent common ancestor with ancient Middle and Upper Yellow River populations around the Early and Middle Holocene.In addition,the connections between Tibetans and Northeastern Asians vary over the past 4000 years,with a stronger matrilineal connection between the two during 4000 BPe3000 BP,and a weakened connection after 3000 BP,that are coincident with climate change,followed by a reinforced connection after the Tubo period(1400 BPe1100 BP).Besides,an over 4000-year matrilineal continuity is observed in some of the maternal lineages.We also find the maternal genetic structure of ancient Tibetans is correlated to the geography and interactions between ancient Tibetans and ancient Nepal and Pakistan populations.Overall,the maternal genetic history of Tibetans can be characterized as a long-term matrilineal continuity with frequent internal and external population interactions that are dynamically shaped by geography,climate changes,as well as historical events. | Ganyu Zhang Can Cui Shargan Wangdue Hongliang Lu Honghai Chen Lin Xi Wei He Haibing Yuan Tinley Tsring Zujun Chen Feng Yang Tashi Tsering Shuai Li Norbu Tashi Tsho Yang Yan Tong Xiaohong Wu Linhui Li Yuanhong He Peng Cao Qingyan Dai Feng Liu Xiaotian Feng Tianyi Wang Ruowei Yang Wanjing Ping Ming Zhang Xing Gao Yichen Liu Wenjun Wang Qiaomei Fu | 2023 | Journal of Genetics and Genomics2023，50，10： | 1 |
| 5 | Maternal genetic history of southern East Asians over the past 12,000 years显示文摘Southern East Asia,including Guangxi and Fujian provinces in China,is home to diverse ethnic groups,languages,and cultures.Previous studies suggest a high complexity regarding population dynamics and the history of southern East Asians.However,large-scale genetic studies on ancient populations in this region are hindered by limited sample preservation.Here,using highly efficient DNA capture techniques,we obtain 48 complete mitochondrial genomes of individuals from Guangxi and Fujian in China and reconstruct their maternal genetic history over the past 12,000 years.We find a strong connection between southern East Asians dating to~12,000-6000 years ago and present-day Southeast Asians.In addition,stronger genetic affinities to northern East Asians are observed in historical southern East Asians than Neolithic southern East Asians,suggesting increased interactions between northern and southern East Asians over time.Overall,we reveal dynamic connections between ancient southern East Asians and populations located in surrounding regions,as well as a shift in maternal genetic structure within the populations over time. | Yalin Liu Tianyi Wang Xichao Wu Xuechun Fan Wei Wang Guangmao Xie Zhen Li Qingping Yang Peng Cao Ruowei Yang Feng Liu Qingyan Dai Xiaotian Feng Wanjing Ping Bo Miao Yun Wu Yichen Liu Qiaomei Fu | 2021 | Journal of Genetics and Genomics2021，48，10： | 1 |
| 6 | Maternal genetic structure of a neolithic population of the Yangshao culture显示文摘The Yangshao culture is one of the most influential Neolithic cultures in East Asia.The populations associated with the Yangshao culture were widely distributed along the Yellow River basin and were suggested to play a profound role in the present-day East Asians.To date,the population of the Yangshao culture is largely investigated through archaeological studies. | Bo Miao Yichen Liu Wanfa Gu Qingli Wei Qian Wu Wenjun Wang Ming Zhang Manyu Ding Tianyi Wang Juncen Liu Feng Liu Peng Cao Qingyan Dai Ruowei Yang Xiaotian Feng Wanjing Ping Weihong Hou Haibing Yuan Qiaomei Fu | 2021 | Journal of Genetics and Genomics2021，48，8： | 0 |
| 7 | 对比分析黑斑息肉综合征合并大肠癌组织肿瘤相关基因(英文)显示文摘Objective:The aim of the study was to screen the differentially expressed genes of Peutz-Jeghers syndrome (PJS) and colorectal carcinoma (CRC).Methods:This study used cDNA microarray to comparatively analyze the gene expression profiles of 4 cases of PJS combined with colorectal adenocarcinoma vs.normal mucosae.The cDNA microarray contained 8064 human genes,and then using RT-PCR to test three genes of all.Results:The experimental data showed that fourteen genes were differentially expressed,which were up-regulated in PJS.Fifty-one genes expressions were altered in CRCs,of which 32 were up-regulated,as compared to the normal mucosae.In addition,5 genes were similarly altered in both PJS and CRCs.RT-PCR analyses confirmed the cDNA microarray data for three of those genes:LATS2,APC and MADH4.Conclusion:LCN2,USP4,GRO3,HYAL1 and APC-these differentially expressed genes likely represent biomarkers for early detection of CRC and may be potential therapeutic targets. | Xiaosan Zhu Yichen Dai Qingzhen Nan Zhangxin Chen Junpei Xie Wei Zeng Yuka Fu Yuanyuan Lin Qingna Lian Qiaofang Sang Xiaojuan Zhan | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，8： | 0 |
| 8 | Interaction between ASEM and Regional Organizations In Asia-Pacific——Concurrently on the development of new regionalism显示文摘In the late 1980s and early 1990s, the once quiet regionalism was on the upsurge again along with the disintegration of the bi-polar world structure and the advent of globalization. It has developed in a deep and multi-dimensional way and is hence called by academics 'new regionalism'. The Asia-Europe Meeting (ASEM), the 10+3 mechanism and the Asia-Pacific Economic Cooperation (APEC) are all cooperation organizations born in this wave of new regionalism. These organizations, with East | Pang Guang is Professor at Institute of European and Asian Studies, Shanghai Academy of Social Sciences. Dai Yichen is Master at Institute of European and Asian Studies, Shanghai Academy of Social Sciences. | 2006 | China International Studies2006，26，4： | 0 |
| 9 | Ancient DNA reveals the maternal genetic history of East Asian domestic pigs显示文摘Zoo-archaeological and genetic evidence suggest that pigs were domesticated independently in Central China and Eastern Anatolia along with the development of agricultural communities and civilizations.However,the genetic history of domestic pigs,especially in China,has not been fully explored.In this study,we generate 42 complete mitochondrial DNA sequences from~7500-to 2750-year-old individuals from the Yellow River basin.Our results show that the maternal genetic continuity of East Asian domestic pigs dates back to at least the Early to Middle Neolithic.In contrast,the Near Eastern ancestry in European domestic pigs saw a near-complete genomic replacement by the European wild boar.The majority of East Asian domestic pigs share close haplotypes,and the most recent common ancestor of most branches dates back to less than 20,000 years before present,inferred using new substitution rates of whole mitogenomes or combined protein-coding regions.Two major population expansion events of East Asian domestic pigs coincided with changes in climate,widespread adoption of introduced crops,and the development of agrarian societies.These findings add to our understanding of the maternal genetic composition and help to complete the picture of domestic pig evolutionary history in East Asia. | Ming Zhang Yichen Liu Zhipeng Li Peng Lü Jacob D.Gardner Maolin Ye Jihuai Wang Miaomiao Yang Jing Shao Weilin Wang Qingyan Dai Peng Cao Ruowei Yang Feng Liu Xiaotian Feng Lizhao Zhang Enwei Li Yunzheng Shi Zehui Chen Shilun Zhu Weiwei Zhai Tao Deng Ziyuan Duan E.Andrew Bennett Songmei Hu Qiaomei Fu | 2022 | Journal of Genetics and Genomics2022，49，6： | 0 |
| 10 | Energy Politics and Security Concepts from Multidimensional Perspectives显示文摘Even though the foregoing theories on international relations give different interpretations on this issue,they are actually interactional and overlapping.Also they seem to share more and more common features.With the acceleration of economic globalization since the end of the Cold War,nation states are increasingly interdependent in the energy sector while competition and coordination become the mainstream of international energy politics.Consequently all parties have put growing importance on international energy cooperation and its institutional construction.They believe that energy security should be achieved through international cooperation and coordination,for it is closely linked with sustainable development of economy and society as well as the external political and economic relations of nation states. | YU Jianhua DAI Yichen | 2012 | Asian Journal of Middle Eastern and Islamic Studies2012，6，4： | 0 |
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