|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Andrographolide and Its Derivative Potassium Dehydrographolide Succinate Suppress PRRSV Replication in Primary and Established Cells via Differential Mechanisms of Action显示文摘Porcine reproductive and respiratory syndrome virus(PRRSV) continues to cause significant economic loss worldwide and remains a serious threat to the pork industry. Currently, vaccination strategies provide limited protection against PRRSV infection, and consequently, new antiviral strategies are urgently required. Andrographolide(Andro) and its derivative potassium dehydrographolide succinate(PDS) have been used clinically in China and other Asian countries as therapies for inflammation-related diseases, including bacterial and viral infections, for decades. Here, we demonstrate that Andro and PDS exhibit robust activity against PRRSV replication in Marc-145 cells and primary porcine alveolar macrophages(PAMs). The two compounds exhibited broad-spectrum inhibitory activities in vitro against clinically circulating type 2 PRRSV GD-HD, XH-GD, and NADC30-like HNhx strains in China. The EC_(50)values of Andro against three tested PRRSV strain infections in Marc-145 cells ranged from 11.7 to 15.3 lmol/L, with selectivity indexes ranging from 8.3 to10.8, while the EC_(50)values of PDS ranged from 57.1 to 85.4 lmol/L, with selectivity indexes ranging from 344 to 515.Mechanistically, the anti-PRRSV activity of the two compounds is closely associated with their potent suppression on NFj B activation and enhanced oxidative stress induced by PRRSV infection. Further mechanistic investigations revealed that PDS, but not Andro, is able to directly interact with PRRSV particles. Taken together, our findings suggest that Andro and PDS are promising PRRSV inhibitors in vitro and deserves further in vivo studies in swine. | Lizhan Su Yarou Gao Mingxin Zhang Zexin Liu Qisheng Lin Lang Gong Jianying Guo Lixia Chen Tongqing An Jianxin Chen | 2021 | Virologica Sinica2021，36，6： | 4 |
| 2 | Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinofl agellate bloom显示文摘Abstract On May 24–29,2019,a harmful algal bloom occurred in Pingtan coastal areas,Fujian,southeast China,and caused mass mortality of cage-cultured fi sh.Two clonal cultures of an unknown naked dinofl agellate were set up from seawater samples taken during the bloom.The cultures were examined for morphological features,ultrastructure characters,photosynthetic pigments,the large subunit(LSU)of the rRNA gene,and the internal transcribed spacer(ITS)sequences,as well as acute toxicity analysis.The cell was unarmored,small-sized,and ovoid,and was characterized by elaborate striations on the epicone and hypocone.The nucleus was large,ellipsoid to oval or kidney-shaped,and centrally located in the cell.A long linear apical groove originated above the sulcus in the ventral epicone and extended to the dorsal side.An elongate,slit-like“ventral pore”was located on the left of the epicone,well away from the apical groove.The chloroplasts were yellowish brown,numerous,band like,and irregularly distributed in the cell periphery.Fucoxanthin was the main accessory pigment composition.Phylogeny topology reconstructed on partial LSU rDNA showed that the unknown dinofl agellate branched as a sister species to Karlodinium sp.(strain IFR981&IFR797,from France)and Karlodinium corrugatum(strain KDGSO08,from Australia),with genetic divergences of 0.6%and 3.3%,respectively.Based on the morphological and molecular phylogenetic analysis,we describe the novel dinofl agellate as Karlodinium elegans sp.nov.A toxicity assay revealed that the clonal culture of K.elegans(strain PTB601)had no adverse eff ect on brine shrimp(Artemia salina)and marine medaka(Oryzias melastigma),indicating it may not be a toxic species. | Jingyi CEN Jianyan WANG Lifen HUANG Yarou LIN Guangmao DING Yuzao QI Songhui LÜ | 2021 | Journal of Oceanology and Limnology2021，39，1： | 1 |
| 3 | Flowering-time regulation by the circadian clock:From Arabidopsis to crops显示文摘Precise timing of flowering in plants is critical for their growth and reproductive processes.One factor controlling flowering time is the cycle of light and darkness within a day,known as the photoperiod.Plants are classified into long-day,short-day,and day-neutral plants based on light requirements for floral initiation.Although the molecular mechanisms that govern this differentiation remain incompletely understood,studies have consistently shown that the circadian clock plays a central role in regulating photoperiod response across diverse plant species.However,there is a scarcity of reviews describing the regulatory network linking the circadian clock with photoperiodic flowering.This review summarizes that regulatory network,focusing on the distinct roles of clock genes in long-day and short-day plants.We also discuss the strategies of clock gene mutations contributing to geographic variation in longday and short-day crops. | Mingkang Yang Wenjie Lin Yarou Xu Biyu Xie Baiyin Yu Liang Chen Wei Huang | 2024 | The Crop Journal2024，12，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员