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| 1 | The Matrix Stiffness and Physical Confinement of Hydrogel Microchannel Jointly Induce the Mesenchymal-Amoeboid Transition for Cancer Cell Migration显示文摘The migration mode transition of cancer cell enhances its invasive capability and the drug resistance,where physical confinement of cell microenvironment has been revealed to induce the mesenchymal-amoeboid transition(MAT).However,most existing studies are performed in PDMS microchannels,of which the stiffness is much higher than that of most mammalian tissues.Therefore,the amoeboid migration transition observed in these studies is actually induced by the synergistic effect of matrix stiffness and confinement.Since the stiffness of cell microenvironment has been reported to influence the cell migration in 2D substrate,the decoupling of stiffness and confinement effects is thus in need for elucidating the underlying mechanism of MAT.However,it is technically challenging to construct microchannels with physiologically relevant stiffness and channel size,where existing microchannel platforms with physiological relevance stiffness are all with>10μm channel width.Such size is too wide to mimic the physical confinement that migrating cancer cells confront in vivo,and also larger than the width of PDMS channel,in which the MAT of cancer cell was observed.Therefore,an in vitro cell migration platform,which could mimic both stiffness and confinement of the native physical microenvironment during cancer metastasis,could profoundly contribute to researches on cancer cell migration and cellular mechanotransduction.In this paper,we overcome the limitations of engineering soft materials in microscale by combining the collagen-alginate hydrogel with photolithography.This enables us to improve the accuracy of molded microchannel,and thus successfully construct a 3D microchannel platform,which matches the stiffness and width ranges of native environmental confinement that migrating cancer cells confront in vivo.The stiffness(0.3~20 kPa),confinement(channel width:3.5~14μm)and the adhesion ligand density of the microchannel can be tuned independently.Interestingly,using this platform,we observed that the migration speed of cancer cell is influenced by the synergistic effect of channel stiffness and width,and the increasing stiffness reverses the effect of channel width on the migration speed of cancer cells.In addition,MAT has a strong correlation with the channel stiffness.These findings make us reconsider the widely accepted hypothesis:physical confinement can induce MAT.Actually,this transition can only occur in stiff confined microenvironment not in soft one.For soft microchannels,the compliance of the channel walls could cause little cell/nucleus deformation,and the MAT could not be induced.To further investigate the mechanism of MAT,we developed a computational model to simulate the effect of nucleus deformation on MAT.With the model,we found that deforming the cell nuclear by decreasing the nucleus stiffness will reduce the cellmigration speed.This implies that nuclear stiffness plays an important role in the regulation of cancer migration speed and thus MAT in microchannels.The effect of channel stiffness on MAT and migration speed as observed in our experiment could partially explain previous findings reported in the literature,where the increasing matrix stiffness of tumor microenvironment promotes cancer metastasis.Our observations thus highlight the critical role of cell nuclear deformation not only in MAT,but also in regulating cellular mechanotransduction and cell-ECM interactions.This developed platform is capable of mimicking the native physical microenvironment during metastasis,providing a powerful tool for high-throughput screening applications and investigating the interaction between cancer migration and biophysical microenvironment. | Meng Wang Bo Cheng Yaowei Yang Han Liu Guoyou Huang Fei Li Feng Xu | 2019 | 医用生物力学2019，34，A01： | 2 |
| 2 | Enhancement of the immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin 2显示文摘 | Jianrong Li Xueya Liang Yaowei Huang | 2004 | Virology2004，329，： | 1 |
| 3 | Generation of VP5 deficient mutant of infectious bursal disease virus strain HZ2显示文摘Infectious bursal disease virus (IBDV) is a bi-segmented, dsRNA virus of the Birnaviridae family. The nonstructural protein VP5 has been re- ported to be associated with virus-induced cell apoptosis and pathogenicity, but its role in viral rep- lication has not been unequivocally identified. Based on a PCR introduced mutagenesis strategy, the 33 bp of 96―129 bp located between ORF A1and ORF A2 of genomic segment A of IBDV strain HZ2 were de- leted, and an Nhe I (GcTaGc) site was inserted at 96―102 bp simultaneously. The mutated segment A was ligated into pCI, resulting in pCI-ANhe3. A chi- meric and deficient IBDV strain, named strain ANhe3, was recovered from chicken embryo fibroblast (CEF) cells by co-transfection with pCI-ANhe3 and pCI-mB, derived from the genomic segment B strain HZ2. The indirect fluorescent assay identified that strain ANhe3 could replicate on CEF cells without expression of VP5. Further examination showed that the patho- genesis of strain ANhe3 replicating on SPF chicken embryos was attenuated compared to strain HZ2. This paper provides a new rapid rescue strategy for gene-deleted virus. This strategy lays a basis for gene-deleted vaccine of IBDV. | LI Long WEI Yongwei HUANG Yaowei YU Lian | 2006 | Chinese Science Bulletin2006，51，15： | 1 |
| 4 | Plasmid DNA encoding antigens of infectious bursal disease viruses induce protective immune responses in chickens: factors influenc- ing efficacy 显示文摘 | Jianrong Li Yaowei Huang Xueya Liang | 2003 | Virus 1Les2003，98，1： | 1 |
| 5 | Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats显示文摘 | Yumin Zhu Xiaoming Yu Yuanshu Zhang Yanyan Ni Fusheng Si Ruisong Yu Shijuan Dong Yaowei Huang Zhen Li | 2013 | Veterinary Microbiology2013，，： | 1 |
| 6 | A CRISPR activation screen identifies genes that enhance SARS-CoV-2 infection显示文摘Dear Editor,Identifying the host factors that are utilized for virus infection and mapping their cell-type expression profile can help to understand the viral tissue/organ tropism and pathogenesis.Much effort has been devoted to the identification of SARS-CoV-2 infection-dependent host factors.CRISPR-based activation(Konermann et al.,2015). | Fei Feng Yunkai Zhu Yanlong Ma Yuyan Wang Yin Yu Xinran Sun Yuanlin Song Zhugui Shao Xinxin Huang Ying Liao Jingyun Ma Yuping He Mingyuan Wang Longhai Tang Yaowei Huang Jincun Zhao Qiang Ding Youhua Xie Qiliang Cai Hui Xiao Chun Li Zhenghong Yuan Rong Zhang | 2023 | Protein & Cell2023，14，1： | 0 |
| 7 | Formation and mechanical properties of Zr-based bulk metallic glass composites with high oxygen levels显示文摘Zr-based bulk metallic glass(BMG) composites with in situ formed Y 2 O 3 particle reinforcements were synthesized by proper additions of Y to ultrahigh-oxygen-containing glass-forming alloy precursors.Microstructures,thermal stabilities,and mechanical properties of the composites were investigated.Glass formation was greatly enhanced by Y additions in the alloys and the resultant particles were homogenously distributed in the glassy matrix,allowing for the fabrication of oxide dispersion strengthened BMG composites.The compressive strength and hardness increased by 10% and 20%,respectively,with the introduction of Y 2 O 3 particles.These results are significant for the design and production of Zr-based BMGs and BMG composites with improved properties using commercial high-oxygen content raw materials under industrial conditions. | LIU ZengQian YANG YaoWei LI Ran HUANG Lu ZHANG Tao | 2012 | Chinese Science Bulletin2012，57，30： | 0 |
| 8 | Purification and characterization of a cold-active myrosinase from marine Pseudomonas oleovorans SuMy07显示文摘Myrosinase(EC 3.2.1.147),also known asβ-thioglucoside glucohydrolase,was first discovered in mustard seeds.Myrosinase hydrolyzes glucosinolate to produce D-glucose and an unstable aglycon,which upon rearrangement yields metabolites such as isothiocyanate,sulfate,acetonitrile,and thiocyanate.Isothiocyanate has a wide range of biological functions[1].Previous studies have shown that isothiocyanate has insect-repellant and antibacterial effects[2].Isothiocyanate also has anti-inflammatory and antioxidant properties,making it a potential anticancer compound[3,4].Sulforaphane is one of the most representative isothiocyanate substances against numerous cancers.Myrosinase extraction is technically challenging,although myrosinase metabolites have a variety of uses.At present,myrosinase is primarily extracted from white mustard seeds after a time-consuming and expensive preparation.Therefore,the search for myrosinase that can hydrolyze glucosinolates to produce isothiocyanate efficiently and economically has become an urgent task.Microbes can secrete myrosinase with rapid growth,lack of seasonal variation,simple intensive cultivation,and simple product extraction. | Zhifa Huang Nannan Liu Yaowei Fang Xiaoyue Hou Guang Yang Jing Lu Haoyu Mi Qinwen Ye Rongjun Zhu Shu Liu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，4： | 0 |
| 9 | Management of traumatic peripheral artery pseudoaneurysm:A 10-year experience at a single center显示文摘Purpose: This study aimed to report our 10-year experience with the management of iatrogenic(penetrating trauma) and traumatic(blunt or penetrating trauma) peripheral artery pseudoaneurysms, based on data from a tertiary referral center.Methods: From January 2012 to December 2021, the medical records of consecutive patients with iatrogenic and traumatic peripheral artery pseudoaneurysms were retrospectively reviewed. Patient demographics, clinical features, imaging data, treatment details, and follow-up results were analyzed.Results: Sixty-one consecutive patients were included in this study;48(79%) were men and 13(21%) women,with a mean age of 49.4 ± 13.4 years(range 24–73 years). There were 42 patients(69%) who underwent open surgery, 18(29%) undergoing endovascular embolization or stent implantation, and one(2%) undergoing ultrasound-guided thrombin injection. All patients successfully underwent open or interventional treatment. The median follow-up was 46.8 months(2.5–117.9 months), and the overall reintervention rate was 10%. Of these,one(5%) patient in the interventional treatment group and five(12%) patients in the open surgery group underwent reintervention. The overall complication rate was 8%, with complications occurring only in the open surgery group. No deaths occurred in the peri-operative period. No late complications, such as thrombosis or pseudoaneurysm recurrence, were observed.Conclusion: Peripheral artery pseudoaneurysms arising from iatrogenic or traumatic causes can be effectively treated by both open surgery and interventional procedures in selected patients with acceptable mid-and long-term outcomes. | Yingliang Wang Hai Zheng Wei Yao Shuguang Ju Yaowei Bai Chaoyang Wang Chen Zhou Jiacheng Liu Chongtu Yang Songjiang Huang Tongqiang Li Yang Chen Bin Xiong | 2023 | Journal of Interventional Medicine2023，6，1： | 0 |
| 10 | Enrichment of anchoring sites by introducing supramolecular halogen bonds for the efficient perovskite nanocrystal LEDs显示文摘Considering the multi-functionalization of ligands,it is crucial for ligand molecular design to reveal the landscape of anchoring sites.Here,a typical triphenylphosphine(TPP)ligand was employed to explore its effect on the surface of CsPbI_(3)perovskite nanocrystals(PNCs).Except for the conventionally considered P-Pb coordination,an P-I supramolecular halogen bonding was also found on the NC surface.The coexistence of the above two types of bonding significantly increased the formation energy of iodine vacancy defects and improved the photoluminescence quantum yield of PNCs up to 93%.Meanwhile,the direct interaction of P and I enhanced the stability of the Pb-I octahedra and dramatically inhibited the migration of I ions.Furthermore,the introduction of additional benzene rings(2-(Diphenylphosphino)-biphenyl(DPB))increased the delocalized properties of the PNC surface and significantly improved the charge transport of the PNCs.As a result,the DPB passivated CsPbI_(3)NCs based top-emitting LEDs exhibite a peak external quantum efficiency(EQE)of 22.8%,a maximum luminance of 15,204 cd m^(−2),and an extremely low-efficiency roll-off of 2.6%at the current density of 500 mA cm^(−2). | Po Lu Ting Li Min Lu Cheng Ruan Siqi Sun Zhennan Wu Yuan Zhong Fujun Zhang Yanbo Gao Yaowei Huang Yang Wang Junhua Hu Fengping Yan Yu Zhang | 2023 | Light(Science & Applications)2023，12，10： | 0 |
| 11 | Lck/Hck/Fgr介导的TBK1酪氨酸磷酸化负调TBK1功能及抗病毒天然免疫应答显示文摘抗病毒天然免疫通路的激活,传统上认识是由蛋白泛素化和蛋白丝苏氨酸磷酸化所介导,对于蛋白酪氨酸磷酸化修饰的作用与生理功能,未有深入探索和认知。课题组发现病毒感染过程中,在多种器官来源的细胞中都观察到细胞内源TBK1有动态的酪氨酸磷酸化修饰,并与TBK1激活与抑制紧密相关。 | Shengduo Liu Shasha Chen Xinran Li Shiying Wu Qian Zhang Qiuheng Jin Lin Hu Ruyuan Zhou Zhengyang Yu Fansen Meng Siwen Wang Yaowei Huang Sheng Ye Li Shen Zongping Xia Jian Zou Xin-Hua Feng 徐平龙 | 2018 | 科学新闻2018，0，4： | 0 |
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