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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Effective and precise adenine base editing n mouse zygotes显示文摘 | Puping Liang Hongwei Sun Xiya Zhang Xiaowei Xie Jinran Zhang Yaofu Bai Xueling Ouyang Shengyao Zhi Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2018 | Protein & Cell2018，9，9： | 4 |
| 3 | Biotinylated dextran amine anterograde tracing of the canine corticospinal tract显示文摘In this study, biotinylated dextran amine (BDA) was microinjected into the left cortical motor area of the canine brain. Fluorescence microscopy results showed that a large amount of BDA-labeled pyramidal cells were visible in the left cortical motor area after injection. In the left medulla oblongata, the BDA-labeled corticospinal tract was evenly distributed, with green fluorescence that had a clear boundary with the surrounding tissue. The BDA-positive corticospinal tract entered into the right lateral funiculus of the spinal cord and descended into the posterior part of the right lateral funiculus, close to the posterior horn, from cervical to sacral segments. There was a small amount of green fluorescence in the sacral segment. The distribution of BDA labeling in the canine central nervous system was consistent with the course of the corticospinal tract. Fluorescence labeling for BDA gradually diminished with time after injection. Our findings indicate that the BDA anterograde tracing technique can be used to visualize the localization and trajectory of the corticospinal tract in the canine central nervous system. | Xiao Han Guangming Lv Huiqun Wu Dafeng Ji Zhou Sun Yaofu Li Lemin Tang | 2012 | Neural Regeneration Research2012，7，11： | 2 |
| 4 | IEEE Transactions on Magnet显示文摘 | Yue Ming Zhang Jiuxing Xiao Yaofu | 2003 | 39(6): 35512003，39，6： | 1 |
| 5 | Research on the best feeding-stuff pattern of room-meat sheep and planting structure显示文摘 | Wang Yaofu Si Deng danba Gu Kuanhe | 2005 | Chinese Journal of Animal Science2005，，2： | 1 |
| 6 | Platelet‐derived growth factor promotes the proliferation of human umbilical cord‐derived mesenchymal stem cells显示文摘 | Pubin Qiu Wencong Song Zhiwei Niu Yaofu Bai Wei Li Shaohui Pan Sha Peng Jinlian Hua | 2012 | Cell Biochem Funct2012，，2： | 1 |
| 7 | 显示文摘 | Yue Ming Zhang Jiuxing Xiao Yaofu | 2003 | IEEE Transaction on Magnetics2003，39，： | 1 |
| 8 | Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation显示文摘Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for protein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embryonic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-inducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChIP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development. | Guang Shi Yaofu Bai Xiya Zhang Junfeng Su Junjie Pang Quanyuan He Pengguihang Zeng Junjun Ding Yuanyan Xiong Jingran Zhang Jingwen Wang Dan Liu Wenbin Ma Junjiu Huang Zhou Songyang | 2022 | Protein & Cell2022，13，10： | 1 |
| 9 | Modified HDDR Process for Producing Anisotropic NdFeB Powders显示文摘The effect of HDDR (hydrogenation-decompositon-desorption recombination process) treating conditions on magnetic ani- sotropy of Nd13FebalCo10B7Ga1Zr0.1 powders has been studied. It is found that increasing recombination temperature is effective for magnetic anisotropy formation, but at the cost of reducing the coercive force. The microstfucture analysis (TEM) indicates that the NdFeB recombination nucleation takes place markedly during the transition range from decomposition stage to recombination stage, in which both temperature and H2 pressure change sharply. In order to create a more stable treatment condition for recombination nucleation, a modified HDDR process is established in which the conventional continuous transition range is divided into several stages. The experi- mental results have proved that such a process can effectively enhance the remanence of the magnet powders with high certainty, while only cause a little reduction in coercivity. | Xubo Liu, Yue Ming, Yaofu Xiao, Qi Zeng, Zhengyi Zhang, Run Wang (Material Science and Engineering School, University of Science and Technology Beijing, Beijing 100083, China) (Beijing Institute of Aeronautical Materials, Beijing 100095, China) | 2001 | Journal of University of Science and Technology Beijing2001，8，2： | 1 |
| 10 | Detectionof3nitrotyrosine,glyco-sylatedhemoglobinandotherbiochemicalindicatorsinserum ofpatientswithtypeⅡ diabetesanditsclinicalsignificance显示文摘 | XinLIU AngLI YaoFU etal | 2012 | JournalofBeihuaUniversity(NaturalScience)2012，3，1： | 1 |
| 11 | Conversion of Levulinic Acid and Formic Acid into γ‐Valerolactone over Heterogeneous Catalysts显示文摘 | LiDeng YanZhao JiangLi YaoFu BingLiao Qing‐XiangGuo | 2010 | ChemSusChem2010，，10： | 1 |
| 12 | TRANSFORMATION OF Nd_(14)Fe_(73)Co_6B_7 ALLOY UNDER HYDROGEN TREATMENT显示文摘The phase constitution and variation of Nd14Fe73Co6B7 alloy after hydriding and subsequentdehydriding treatment at 770℃ have been investigated respectively by means of X-raydiffraction,SEM,TEM and magnetic measurement techniques.The original coarse,as castgrain structure,is dissolved into α-Fe,Nd hydride and Fe2B mixture with average dimensionof 0.1 μm,during hydriding process,and subsequently the triphase mixture recombined intofinely unique Nd2Fe14B phase with dimension of 0.3 μm by desorbing the hydrogen.It wasfound that the Nd-rich phase distributed at the boundary of as-cast grain structure diffusedinto matrix Nd2Fe14B grain during hydriding.It remains during dehydriding and distributehomogeneously around some colonies composed of fine grains of Nd2Fe14B. | LIU Jian QIU Baoqin LIU Muyi XIAO Yaofu University of Science and Technology Beijing,Beijing,China associate professor,Department of Materials,University of Science and Technology Beijing,Beijing 100083,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，6： | 1 |
| 13 | A new electrolytic method of preparing ultrafine metallic powders显示文摘 | He Feng Zhang Zhengyi Xiao Yaofu Wang Wuxiang Han Yafang Wang Run | 2002 | Journal of Materials Processing Tech2002，，1： | 1 |
| 14 | A New kind of NdFeB magnet prepared by spark plasma sintering显示文摘 | ZHANG Jiuxing XIAO Yaofu | 2003 | IEEE Trans On Magnetics2003，39，6： | 1 |
| 15 | The P124A mutation of SRP14 alters its migration on SDS-PAGE without impacting its function显示文摘SRP14 is a crucial protein subunit of the signal recognition particle(SRP),a ribonucleoprotein complex essential for co-translational translocation to the endoplasmic reticulum.During our investigation of SRP14 expression across diverse cell lines,we observe variations in its migration on sodium dodecyl sulfate-polyacrylamide gel electro-phoresis(SDS-PAGE),with some cells exhibiting slower migration and others migrating faster.However,the cause of this phenomenon remains elusive.Our research rules out alternative splicing as the cause and,instead,iden-tifies the presence of a P124A mutation in SRP14(SRP14P124A)among the faster-migrating variants,while the slower-migrating variants lack this mutation.Subsequent ectopic expression of wild-type SRP14P124 or SRP14WT and SRP14P124A in various cell lines confirms that the P124A mutation indeed leads to faster migration of SRP14.Further mutagenesis analysis shows that the P117A and A121P mutations within the alanine-rich domain at the C-terminus of SRP14 are responsible for migration alterations on SDS-PAGE,whereas mutations outside this domain,such as P39A,Y27F,and T45A,have no such effect.Furthermore,the ectopic expression of SRP14WT and SRP14P124A yields similar outcomes in terms of SRP RNA stability,cell morphology,and cell growth,indicating that SRP14P124A represents a natural variant of SRP14 and retains comparable functionality.In conclusion,the substitution of proline for alanine in the alanine-rich tail of SRP14 results in faster migration on SDS-PAGE,but has little effect on its function. | Yaofu Liu Jinqiu Zhou | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 16 | A NOVEL ROUTE FOR MAKING COERCIVE NdFeB POWDER——HDD PROCESS显示文摘NdFeB coercive powder can be obtained directely by hydrogen treated ahoy ingots,The magnetic properties of the powder are comparable with that made by rapidsolidifiedprocess.An appreciable coercivity created during hydrogen treatment is closely relatedto the refined crystal grains. | XIAO Yaofu ZHANG Zhengyi TANG Weizhong University of Science and Technology Beijing,Beijing,China Associate Professor,University of Science and Technology Beijing,Beijing 100083,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，7： | 0 |
| 17 | The environmental effect of technical change:A spatial model with city-level data in China显示文摘This paper studies the environmental effects of technical change using a spatial model with panel data from 284 prefecture-cities over 2004-2015 in China.We find that the effects of technical change vary across different dimensions of technical change and different pollution indicators.Furthermore,we also provide robust evidence for the existence of the spatial effects of technical change on environmental pollution across cities.First,indigenous technical change displays three patterns of effects on the four pollutants:a positive effect on wastewater,a negative effect on PM_(2.5)concentrations,and an inverted U-shaped relationship with SO_(2)and soot emissions.The spatial effect of indigenous technical change promotes cleaner industrial productions(fewer emissions of SO_(2),soot and wastewater)but higher PM_(2.5)concentrations.Second,technology transfers from foreign direct investment are associated with less pollution except for wastewater,and their spatial effects are unanimously negative on all pollutants.Finally,absorptive capacity can also promote cleaner industrial production,but its spatial effects can do otherwise.Accordingly,the government should take the spatial spillover effects of technical change into account when implementing specific policies,pin down specific pollutants to make full use of the pollution-reducing effects of technical change,and improve the absorptive capacity of domestic firms. | Peng Li Yaofu Ouyang Dan Shi | 2021 | Chinese Journal of Population,Resources and Environment2021，19，1： | 0 |
| 18 | Recent advances in the syntheses of pyrroles显示文摘The pyrrole moiety is an important structural motif in functional materials,natural products,and pharmaceuticals.More and more synthetic strategies toward pyrroles have emerged,where various efficient building blocks are developed and these synthons enable the syntheses of pyrroles with different numbers of components.However,no review specifically summarizes the syntheses of pyrroles according to the type and number of employed building blocks.To aid researchers to design appropriate substrates for pyrrole synthesis,herein we summarized the advances in pyrrole syntheses and classified these reactions into four categories according to the number of employed components,which may shed light on developing more efficient synthetic methods toward substituted pyrroles. | Tao Shi Gaofeng Yin Xiaodong Wang Yongxia Xiong Yan Peng Shuang Li Yaofu Zeng Zhen Wang | 2023 | Green Synthesis and Catalysis2023，4，1： | 0 |
| 19 | Anisotropic characteristic in HDDR Nd_(13)Fe_(80)B_7显示文摘The function of disproportionation and desorption stages of hydrogenation-disproportionation-desorption-recombination(HDDR) process in the formation of magnetic anisotropy was studied. The results showed that degree of anisotropy (DOA) ofNd12Fe80B7 induced by appropriate HDDR process is comparable with that of Nd13Fe80-xB7M1 (M=Co, Zr or Ga), however, the moder-ate coercive force of Nd13Fe80B7 magnetic powder led to an undesirable (BH) of 104 kJ/m3 in bonded magnet. Experiments demon-strated that a relatively short disproportionation time, combined with a high desorption temperature and slow desorption rate, duringHDDR process is beneficial to anisotropy attainment, but HDDR Nd13Fe80B7 magnetic powder becomes isotropic if the dispropor-tionation time is longer than a certain period. This phenomenon strongly indicated that the origin of anisotropy in NdFeB-typeHDDR material is contributed by disproportionation stage. XRD (X-ray Diffraction) analysis and TEM (Transmission Electron Mi-croscope) observation told that no other phase except NdH2, α-Fe and Fe2B has been found in the disproportionation step. The for-mation of HDDR Nd13Fe80 B7 anisotropy might be related to the early lamella disproportionation structure. | Jingzhi Han, Aizhi Sun, Yaofu Xiao, Xubo Liu, and Run WangMaterials Science and Engineering School, University of Science and Technology Beijing, Beijing 100083, China | 2003 | Journal of University of Science and Technology Beijing2003，10，3： | 0 |
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