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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Nonlinear vibration response characteristics of a dual-rotor-bearing system with squeeze film damper显示文摘Rolling bearing and Squeeze Film Damper(SFD)are used in rotor support structures,and most researches on the nonlinear rotor-bearing system are focused on the simple rotor-bearing systems.This work emphasizes the comparative analysis of the influence of SFD on the nonlinear dynamic behavior of the dual-rotor system supported by rolling bearings.Firstly,a reduced dynamic model is established by combining the Finite Element(FE)method and the freeinterface method of component mode synthesis.The proposed model is verified by comparing the natural characteristics obtained from an FE model with those from the experiment.Then,the steady-state vibration responses of the system with or without SFD are solved by the numerical integration method.The influences of the ball bearing clearance,unbalance,centralizing spring stiffness and oil film clearance of SFD on the nonlinear steady-state vibration responses of the dual-rotor system are analyzed.Results show that SFD can effectively suppress the amplitude jump of the dual rotor system sustaining two rotors unbalance excitations.As the ball bearing clearance or unbalance increases,the amplitude jump phenomenon becomes more obvious,the resonance hysteresis phenomenon strengthens or weakens,the resonant peaks shift to the left or the right,respectively.SFD with unreasonable parameters will aggravate the system vibration,the smaller the oil film clearance,the better the damping performance of the SFD,the larger the centralizing spring stiffness is,the larger resonance amplitudes are. | Xinxing MA Hui MA Haiqin QIN Xumin GUO Chenguang ZHAO Mingyue YU | 2021 | Chinese Journal of Aeronautics2021，34，10： | 3 |
| 3 | Transcriptome-wide evolutionary analysis on essential brown algae(Phaeophyceae)in China显示文摘Brown algae(Chromista,Ochrophyta,Phaeophyceae) are a large group of multicellular algae that play important roles in the ocean's ecosystem and biodiversity.However,poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field.In this study,we sequenced the de novo transcriptome of 18 major species of brown algae in China,covering six orders and seven families,using the high-throughput sequencing platform Illumina HiSeq 2000.From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum,we identified 108 nuclear-generated orthologous genes and clarified the phylogenetic relationships among these brown algae based on a multigene method.These brown algae could be separated into two clades: Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmarestiale-Fucales.The former was at the base of the phylogenetic tree,indicating its early divergence,while the latter was divided into two branches,with Order Fucales diverging from Orders Ectocarpales,Laminariales,and Desmarestiale.In our analysis of taxonomy-contentious species,Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina,respectively,while Petalonia fascia showed possible relation to genus Scytosiphon.The study provided molecular evidence for the phylogenetic taxonomy of brown algae. | SUN Jing WANG Liang WU Shuangxiu WANG Xumin XIAO Jingfa CHI Shan LIU Cui REN Lufeng ZHAO Yuhui LIU Tao YU Jun | 2014 | Acta Oceanologica Sinica2014，33，2： | 2 |
| 4 | Flesh quality of hybrid grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)fed with hydrolyzed porcine mucosasupplemented low fishmeal diet显示文摘Iso-nitrogenous and iso-lipidic diets containing 0%,3%,6%,9%,and 12%hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition composition,texture property,and gene expression related to muscle growth of hybrid groupers(Epinephelus fuscoguttatus\Epinephelus lanceolatus_).Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days.It was found that the weight gain percentage in the HPM0,HPM3,and HPM6 groups did not differ(P>0.05).The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups(P<0.05).The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0,HPM9,and HPM12 groups(P<0.05).The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups(P<0.05).The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group(P<0.05).The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid,as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups(P<0.05).The relative expressions of gene myogenic factor 5,myocyte enhancer factor 2c,myocyte enhancer factor 2a,myosin heavy chain,transforming growth factor-beta 1(TGF-b1),and follistatin(FST)were the highest in the dorsal muscle of the HPM3 group.The results indicated that the growth performance of hybrid grouper fed a diet with 6%HPM and 27%fish meal was as good as that of the HPM0 group.When fish ingested a diet containing 3%HPM,the expression of genes TGF-β1 and FST involved in muscle growth were upregulated,and then the muscle quality related to hardness and chewiness were improved.An appropriate amount of HPM could be better used in grouper feed. | Xuanyi Yang Xinyan Zhi Ziling Song Guanghui Wang Xumin Zhao Shuyan Chi Beiping Tan | 2022 | Animal Nutrition2022，，1： | 2 |
| 5 | A recombinant cell-permeable p53 fusion protein is selectively stabilized under hypoxia and inhibits tumor cell growth显示文摘 | Zhao Yu Junhua Wu Shaoping Wu Peiyuan Jia Ying Tong Xumin Wu Yuxia Wang | 2009 | Cancer Letters2009，，1： | 1 |
| 6 | A pangenomic study of Bacillus thuringiensis显示文摘杆菌 thuringiensis (B。thuringiensis ) 是一个土壤住所克积极的细菌和它的编码 plasmid 的毒素(叫喊) 通常被用作杀虫剂的生物选择。在 pangenomic 研究,我们定序七 B。thuringiensis 在高范围并且基础质量的使用孤立下一代的定序站台。B。当一个新染色体被增加时, thuringiensis pangenome 被外推有 4196 核心基因和 558 唯一的基因的 asymptotic 价值。比作它一样的类的密切相关的种的 pangenomes, B。thuringiensis pangenome 显示出一个开的特征,类似于 B。仙影拳然而并非到 B。anthracis;后者有关上的 pangenome。我们也在七 B 之中发现了广泛的分叉。thuringiensis 染色体集会,它怀有宽大的重复和单个核苷酸多型性(SNP ) 。在 orthologous 基因之中的身份比 84.5% 大,为染色体变化的热点在 2.32.8 Mb 和 5.05.6 Mb 的 genomic 区域被发现。我们断定在所有差距被关上以前,从多重种类的高范围的顺序集会为 pangenomic 研究是很有用的。 | Yongjun Fang Zhaolong Li Jiucheng Liu Changlong Shu Xumin Wang Xiaowei Zhang Xiaoguang Yu Duojun Zhao Guiming Liu Songnian Hu Jie Zhang Ibrahim Al-Mssallem Jun Yu | 2011 | Journal of Genetics and Genomics2011，38，12： | 1 |
| 7 | Compact logic operator utilizing a single-layer metasurface显示文摘In this paper, we design and demonstrate a compact logic operator based on a single-layer metasurface at microwave frequency. By mapping the nodes in the trained fully connected neural network(FCNN) to the specific unit cells with phase control function of the metasurface, a logic operator with only one hidden layer is physically realized. When the incident wave illuminates specific operating regions of the metasurface, corresponding unit cells are activated and can scatter the incident wave to two designated zones containing logical information in the output layer. The proposed metasurface logic operator is experimentally verified to achieve three basic logic operations(NOT, OR, and AND) under different input signals. Our design shows great application potential in compact optical systems, low-power consumption information transmission, and ultrafast wave-based full signal processing. | Zihan Zhao Yue Wang Xumin Ding Haoyu Li Jiahui Fu Kuang Zhang Shah Nawaz Burokur Qun Wu | 2022 | Photonics Research2022，10，2： | 1 |
| 8 | Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation显示文摘In fluorescence microscopy,computational algorithms have been developed to suppress noise,enhance contrast,and even enable super-resolution(SR).However,the local quality of the images may vary on multiple scales,and these differences can lead to misconceptions.Current mapping methods fail to finely estimate the local quality,challenging to associate the SR scale content.Here,we develop a rolling Fourier ring correlation(rFRC)method to evaluate the reconstruction uncertainties down to SR scale.To visually pinpoint regions with low reliability,a filtered rFRC is combined with a modified resolution-scaled error map(RSM),offering a comprehensive and concise map for further examination.We demonstrate their performances on various SR imaging modalities,and the resulting quantitative maps enable better SR images integrated from different reconstructions.Overall,we expect that our framework can become a routinely used tool for biologists in assessing their image datasets in general and inspire further advances in the rapidly developing field of computational imaging. | Weisong Zhao Xiaoshuai Huang Jianyu Yang Liying Qu Guohua Qiu Yue Zhao Xinwei Wang Deer Su Xumin Ding Heng Mao Yaming Jiu Ying Hu Jiubin Tan Shiqun Zhao Leiting Pan Liangyi Chen Haoyu Li | 2023 | Light(Science & Applications)2023，12，12： | 0 |
| 9 | Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice显示文摘Dear Editor,Duck Tembusu virus(DTMUV)is the causative agent of a new,acute and severe infectious disease in ducks(Su et al.,2011).TMUV was first isolated from Culex tritaeniorhynchus in Malaysia in 1955(Platt et al.,1975),and this strain is regarded as a reference strain.In our previous study,an infectious clone for the mosquito-derived Tembusu virus prototypical strain MM_1775(GenBank:JX477685.2)was constructed,and the rMM_1775 virus was rescued successfully(Wang et al.,2021).And the TMUV strain rCQW1 was rescued by a reverse genetic system using the viral RNAs of CQW1(GenBank:KM233707.1)(Chen et al.,2018;Guo et al.,2020),which was isolated from ducks in 2013(Zhu et al.,2015). | Xiaoli Wang Yu He Jiaqi Guo Zhen Wu Andres Merits Mingshu Wang Renyong Jia Dekang Zhu Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Qun Gao Di Sun Anchun Cheng Shun Chen | 2023 | Virologica Sinica2023，38，5： | 0 |
| 10 | Dynamic characteristics of a shrouded blade with impact and friction显示文摘A simplified computational model of a twisted shrouded blade with impact and friction is established.In this model,the shrouded blade is simulated by a flexible Timoshenko beam with a tip-mass,and the effects of centrifugal stiffening,spin softening,and Coriolis force are considered.Impact force is simulated using a linear spring model,and friction force is generated by a tangential spring model under sticking state and a Coulomb friction model under sliding state.The proposed model is validated by a finite element model.Then,the effects of initial gap and normal preload,coefficient of friction,and contact stiffness ratio(the ratio of tangential contact stiffness to normal contact stiffness)on system vibration responses are analyzed.Results show that resonant peaks become inconspicuous and impact plays a dominant role when initial gaps are large between adjacent shrouds.By contrast,in small initial gaps or initial normal preloads condition,resonant speed increases sharply,and the optimal initial normal preloads that can minimize resonant amplitude becomes apparent.Coefficient of friction affects the optimal initial normal preload,but it does not affect vibration responses when the contact between shrouds is under full stick.System resonant amplitude decreases with the increase of contact stiffness ratio,but the optimal initial normal preload is unaffected. | Xumin GUO Jin ZENG Hui MA Chenguang ZHAO Lin QU Bangchun WEN | 2020 | Frontiers of Mechanical Engineering2020，15，2： | 0 |
| 11 | Na_(2)CO_(3)-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics显示文摘Alkali-salinity exerts severe osmotic,ionic,and high-p H stresses to plants.To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species(ROS)homeostasis,physiological and diverse quantitative proteomics analyses of alkaligrass(Puccinellia tenuiflora)under Na_(2)CO_(3)stress were conducted.In addition,Western blot,real-time PCR,and transgenic techniques were applied to validate the proteomic results and test the functions of the Na_(2)CO_(3)-responsive proteins.A total of 104 and 102 Na_(2)CO_(3)-responsive proteins were identified in leaves and chloroplasts,respectively.In addition,84 Na_(2)CO_(3)-responsive phosphoproteins were identified,including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts,which are crucial for the regulation of photosynthesis,ion transport,signal transduction,and energy homeostasis.A full-length Pt FBA encoding an alkaligrass chloroplastic fructosebisphosphate aldolase(FBA)was overexpressed in wild-type cells of cyanobacterium Synechocystis sp.Strain PCC 6803,leading to enhanced Na_(2)CO_(3)tolerance.All these results indicate that thermal dissipation,state transition,cyclic electron transport,photorespiration,repair of photosystem(PS)Ⅱ,PSI activity,and ROS homeostasis were altered in response to Na_(2)CO_(3)stress,which help to improve our understanding of the Na_(2)CO_(3)-responsive mechanisms in halophytes. | Jinwei Suo Heng Zhang Qi Zhao Nan Zhang Yongxue Zhang Ying Li Baohua Song Juanjuan Yu Jianguo Cao Tai Wang Ji Luo Lihai Guo Jun Ma Xumin Zhang Yimin She Lianwei Peng Weimin Ma Siyi Guo Yuchen Miao Sixue Chen Zhi Qin Shaojun Dai | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 0 |
| 12 | Hankou Customs House to be renovated显示文摘The Hankou Customs House will undergo repair soon!On June 27,the website of Wuhan Public Resources Trading Center issued a notice,announcing that renovation will be carried out on its bell tower and the main building's ground floor.The renovation project covers 169 square meters of the tower and 692 square meters of the ground floor area.The repair will last 45 days. | Wan Xumin Zhao Guangliang | 2022 | Changjiang Weekly2022，，26： | 0 |
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