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| 1 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 2 | MicroRNA 145 may play an important role in uveal melanoma cell growth by potentially targeting insulin receptor substrate-1显示文摘 | Li Yang Huang Qiming Shi Xuehui Jin Xiang Shen Li Xu Xiaolin Wei Wenbin | 2014 | Chinese Medical Journal2014，，8： | 9 |
| 3 | Intravitreal ranibizumab therapy versus photodynamic therapy for idiopathic choroidal neovascularization: a comparative study on visual acuity, retinal and choroidal thickness显示文摘 | Shi Xuehui Wei Wenbin Zhang Cong | 2014 | Chinese Medical Journal2014，，12： | 8 |
| 4 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 5 | Translational Regulation of Plant Response to High Temperature by a Dual-Function tRNAHls Guanylyltransferase in Rice显示文摘As sessile organisms,plants have evolved numerous strategies to acclimate to changes in environmental temperature.However,the molecular basis of this acclimation remains largely unclear.In this study we identified a tRNAHis guanylyltransferase,AET1,which contributes to the modification of pre-tRNAH,s and is required for normal growth under high-temperature conditions in rice.Interestingly,AET1 possibly interacts with both RACK1A and elF3h in the endoplasmic reticulum.Notably,AET1 can directly bind to OsARF mRNAs including the uORFs of OsARF19 and OsARF23,indicating that AET1 is associated with translation regulation.Furthermore,polysome profiling assays suggest that the translational status remains unaffected in the aet1 mutant,but that the translational efficiency of OsARF19 and OsARF23 is reduced;moreover,OsARF23 protein levels are obviously decreased in the aet1 mutant under high temperature,implying that AET1 regulates auxin signaling in response to high temperature.Ourfindings provide new insights into the molecular mechanisms whereby AET1 regulates the environmental temperature response in rice by playing a dual role in tRNA modification and translational control. | Ke Chen Tao Guo Xin-Min Li Yi-Min Zhang Yi-Bing Yang Wang-Wei Ye Nai-Qian Dong Chuan-Lin Shi Yi Kan You-Huang Xiang Hai Zhang Ya-Chao Li Ji-Ping Gao Xuehui Huang Qiang Zhao Bin Han Jun-Xiang Shan Hong-Xuan Lin | 2019 | Molecular Plant2019，12，8： | 4 |
| 6 | MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3显示文摘Central precocious puberty(CPP)refers to a human syndrome of early puberty initiation with characteristic increase in hypothalamic production and release of gonadotropin-releasing hormone(GnRH).Previously,loss-of-function mutations in human MKRN3,encoding a putative E3 ubiquitin ligase,were found to contribute to about 30%of cases of familial CPP.MKRN3 was thereby suggested to serve as a‘brake’of mammalian puberty onset,but the underlying mechanisms remain as yet unknown.Here,we report that genetic ablation of Mkrn3 did accelerate mouse puberty onset with increased production of hypothalamic GnRH1.MKRN3 interacts with and ubiquitinates MBD3,which epigenetically silences GNRH1 through disrupting the MBD3 binding to the GNRH1 promoter and recruitment of DNA demethylase TET2.Our findings have thus delineated a molecular mechanism through which the MKRN3-MBD3 axis controls the epigenetic switch in the onset of mammalian puberty. | Chuanyin Li Wenli Lu Liguang Yang Zhengwei Li Xiaoyi Zhou Rong Guo Junqi Wang Zhebao Wu Zhiya Dong Guang Ning Yujiang Shi Yinmin Gu Peng Chen Zijian Hao Tianting Han Meiqiang Yang Wei Wang Xuehui Huang Yixue Li Shan Gao Ronggui Hu | 2020 | National Science Review2020，7，3： | 2 |
| 7 | Deep genotyping of the gene GmSNAP facilitates pyramiding resistance to cyst nematode in soybean显示文摘Soybean cyst nematode(SCN)is a highly destructive pathogen.The soybean host genome harbors at least two major genes for resistance(rhg1 and Rhg4),as well as a minor locus(SCN3-11).In the present study,a splicing site in GmSNAP11,the potential causal gene of SCN3-11,was identified by comparison of the GmSNAP11 cDNA sequences generated from resistant and susceptible soybean accessions.The sequence information was used to design a codominant CAPS marker,GmSNAP11-2565,which was used to genotype a panel of 209 soybean accessions varying with respect to SCN resistance.Analyses of the effect of the haplotypes formed by GmSNAP11-2565 and another large-effect(nonsynonymous)locus,GmSNAP11-2307,previously identified in GmSNAP11,revealed linkage disequilibrium(P<0.0001)between the two loci,suggesting that GmSNAP11-2565 could be used as a marker for GmSNAP11.GmSNAP11-2565 was accordingly used,along with established markers for GmSNAP18(rhg1)and GmSHMT(Rhg4),to characterize the panel accessions.The mean SCN female index of accessions carrying only the GmSNAP11 allele associated with resistance(20.3%)was higher than that associated with accessions carrying alleles for resistance at both GmSNAP11 and GmSNAP18(12.4%),while the index for accessions carrying alleles for resistance at all of GmSNAP11,GmSNAP18,and GmSHMT was very low(1.9%).Selection on all three markers was effective for maintaining a high level of resistance to SCN race 3. | Yu Tian Bo Liu Xuehui Shi Jochen C.Reif Rongxia Guan Ying-hui Li Li-juan Qiu | 2019 | The Crop Journal2019，7，5： | 2 |
| 8 | Observation and management of retinal changes related to diving in professional divers显示文摘 | Zhou Dan Wei Wenbin Tian Bei Wang Cong Shi Xuehui Jiao Xuan | 2014 | Chinese Medical Journal2014，，4： | 2 |
| 9 | Plant pan-genomics and its applications显示文摘Plant genomes are so highly diverse that a substantial proportion of genomic sequences are not shared among individuals.The variable DNA sequences,along with the conserved core sequences,compose the more sophisticated pan-genome that represents the collection of all non-redundant DNA in a species.With rapid progress in genome sequencing technologies,pan-genome research in plants is now accelerating.Here we review recent advances in plant pan-genomics,including major driving forces of structural variations that constitute the variable sequences,methodological innovations for representing the pan-genome,and major successes in constructing plant pan-genomes.We also summarize recent efforts toward decoding the remaining dark matter in telomere-to-telomere or gapless plant genomes.These new genome resources,which have remarkable advantages over numerous previously assembled less-than-perfect genomes,are expected to become new references for genetic studies and plant breeding. | Junpeng Shi Zhixi Tian Jinsheng Lai Xuehui Huang | 2023 | Molecular Plant2023，16，1： | 1 |
| 10 | Cluster anal- ysis on city real estate market of China: based on a new inte- grated method for time series clustering 显示文摘 | Kun Guo Jue Wang Gushan Shi Xuehui Cao | 2012 | Procedia Corn- puter Science2012，9，： | 1 |
| 11 | Chitosan nanofiber scaffold enhances hepatocyte adhesion and function 显示文摘 | Chu Xuehui Shi Xiaolei Feng Zhangqi | 2009 | Biotechnol Lett2009，31，： | 1 |
| 12 | Does school quality matter?: evidence from a natural experiment in rural China显示文摘 | PARK A SHI Xinzheng HSIEH Chang-tai AN Xuehui | 2008 | Mimeo2008，，： | 1 |
| 13 | Advances on research of coloring mechanisms in citrus fruit显示文摘 | Yang Guoshun Shi Xuehui LIU Kun yu | 2005 | Journal of Hunan Agricultural University2005，2，3： | 1 |
| 14 | Chitosan nanofiber scaffold enhances hepatocyte adhesion and function 显示文摘 | Chu Xuehui Shi Xiaolei Feng Zhangqi | 2009 | Biotechnol Lett2009，31，3： | 1 |
| 15 | Chlorophyll-a concentrations in 82 large alpine lakes on the Tibetan Plateau during 2003–2017: temporal–spatial variations and influencing factors显示文摘As essential parts of the unique ecosystem of Tibetan Plateau(TP),the sizes and associated physical properties of alpine lakes have long been investigated.However,little is known about one of the most critical biogeochemical properties,i.e.the Chlorophyll-a(Chl-a)concentrations.Here,for the first time,we presented a comprehensive investigation of the temporal–spatial variations in Chl-a in 82 lakes(>50 km^(2))across the entire TP region,based on MODIS observations in the period of 2003–2017.The results showed that the 82 lakes exhibited an average longterm mean Chl-a of 3.3±4.3 mg m^(−3),with high Chl-a lakes concentrated in the eastern and southern inner TP basin and northeastern parts of the TP.An interannual trend analysis revealed that lakes exhibiting(significantly)decreasing Chl-a trends and(significantly)increasing Chl-a trends were comparable in numbers but differed in distribution patterns.A correlation analysis indicated that at least 70%of the interannual variability in Chl-a values of lakes was significantly correlated with one of the four environmental factors(wind speed,ice cover duration,lake water surface temperature and surface runoff)and lake size.In addition,glacier meltwater tended to reduce lake Chl-a while salinity levels showed minor influences. | Xuehui Pi Lian Feng Weifeng Li Junguo Liu Xingxing Kuang Kun Shi Wei Qi Deliang Chen Jing Tang | 2021 | International Journal of Digital Earth2021，14，6： | 0 |
| 16 | Beamforming design for RIS-aided amplify-and-forward relay networks显示文摘The use of a reconfigurable intelligent surface(RIS)in the enhancement of the rate performance is considered to involve the limitation of the RIS being a passive reflector.To address this issue,we propose a RIS-aided amplify-and-forward(AF)relay network in this paper.By jointly optimizing the beamforming matrix at AF relay and the phase-shift matrices at RIS,two schemes are put forward to address a maximizing signal-to-noise ratio(SNR)problem.First,aiming at achieving a high rate,a high-performance alternating optimization(AO)method based on Charnes–Cooper transformation and semidefinite programming(CCT-SDP)is proposed,where the optimization problem is decomposed into three subproblems solved using CCT-SDP,and rank-one solutions can be recovered using Gaussian randomization.However,the optimization variables in the CCT-SDP method are matrices,leading to extremely high complexity.To reduce the complexity,a low-complexity AO scheme based on Dinkelbachs transformation and successive convex approximation(DT-SCA)is proposed,where the variables are represented in vector form,and the three decoupling subproblems are solved using DT-SCA.Simulation results verify that compared to three benchmarks(i.e.,a RIS-assisted AF relay network with random phase,an AF relay network without RIS,and a RIS-aided network without AF relay),the proposed CCT-SDP and DT-SCA schemes can harvest better rate performance.Furthermore,it is revealed that the rate of the low-complexity DT-SCA method is close to that of the CCT-SDP method. | Xuehui WANG Feng SHU Riqing CHEN Peng ZHANG Qi ZHANG Guiyang XIA Weiping SHI Jiangzhou WANG | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，12： | 0 |
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