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| 1 | Side population cells isolated from KATO Ⅲ human gastric cancer cell line have cancer stem cell-like characteristics显示文摘AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS: We analyzed the presence of SP cells indifferent human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO Ⅲ human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO Ⅲ human gastric cancer cell line were subcutaneously injected into nude mice. RESULTS: SP cells from the total population accounted for 0.57% in KATO Ⅲ, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice. CONCLUSION: SP cells have some cancer stem celllike characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer. | Jun-Jun She Peng-Ge Zhang Xuan Wang Xiang-Ming Che Zi-Ming Wang | 2012 | World Journal of Gastroenterology2012，18，33： | 20 |
| 2 | Experiment on Leader Propagation Characteristics of Air Gaps in UHVDC Transmission Towers Under Positive Switching Impulse Voltages显示文摘Rapid developments in EHV/UHV transmission systems require a deeper understanding of the mechanism of long air gap discharge.Leader propagation is one of the main processes in long gap breakdown.In this paper,the leader propagation characteristics of real size±800 kV UHVDC transmission tower gaps under positive switching impulse voltages(185/2290μs)are investigated.An integrated observation platform consisting of an impulse voltage divider,a coaxial shunt,a high-speed video camera,and a set of integrated optical electric field sensors(IOES),is established.The waveforms of impulse voltage,discharge current,electric field variation at specific positions,and time-resolved photographs of discharge morphology are recorded.Axial leader velocity and the relationship between leader advancements and injected charge are obtained.The typical value of leader stable propagation velocity is 1.7–2.2 cm/μs,which varies slightly with the gap length and applied voltage amplitude.The leader velocity in the re-illumination process is much higher,and is seen as varying from 5 cm/μs to 30 cm/μs,with an average value around 10 cm/μs.The charge in leader channel per unit length is 20–40μC/m,which illustrates a near-direct proportion relationship between discharge current and leader velocity.The observed parameters are important for further simulation of the tower gap breakdown processes. | Xuan Zhou She Chen Hai Wang Rong Zeng Chijie Zhuang Junjie Yu Yujian Ding | 2015 | CSEE Journal of Power and Energy Systems2015，1，3： | 12 |
| 3 | Progress involving new techniques for liposome preparation显示文摘The article presents a review of new techniques being used for the preparation of liposomes.A total of 28 publications were examined.In addition to the theories,characteristics and problems associated with traditional methods,the advantages and drawbacks of the latest techniques were reviewed.In the light of developments in many relevant areas,a variety of new techniques are being used for liposome preparation and each of these new technique has particular advantages over conventional preparation methods.However,there are still some problems associated with these new techniques that could hinder their applications and further improvements are needed.Generally speaking,due to the introduction of these latest techniques,liposome preparation is now an improved procedure.These applications promote not only advances in liposome research but also the methods for their production on an industrial scale. | Zhenjun Huang Xuan Li Ting Zhang Yanzhi Song Zhennan She Jing Li Yihui Deng | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，4： | 7 |
| 4 | Survey of recent progress on lightning and lightning protection research显示文摘Lightning and lightning induced effects have significant influence on many aspects affecting the public, whichmakes the research of lightning and lightning protection very important. However, due to the strong randomness andcomplex discharge mechanisms of lightning, the understanding of lightning is still far from satisfactory. On the basis ofthe International Conference on Lightning Protection 2014, this study gives a review of recent progress on lightningand lightning protection research covering the following aspects: lightning locating and observation, lightning physics,lightning electromagnetic transients, and lightning protection for various systems. The goals of this study are to givereaders an overall introduction to the recent progress in lightning and lightning protection research, and to motivatethem to conduct further studies to address the unsolved problems in lightning-related research. | Rong Zeng Chijie Zhuang Xuan Zhou She Chen Zezhong Wang Zhanqing Yu Jinliang He | 2016 | High Voltage2016，1，1： | 6 |
| 5 | The anticancer efficacy of pixantrone-loaded liposomes decorated with sialic acid-octadecylamine conjugate显示文摘 | Zhennan She Ting Zhang Xuling Wang Xuan Li Yanzhi Song Xiaobo Cheng Zhenjun Huang Yihui Deng | 2014 | Biomaterials2014，，： | 2 |
| 6 | Eye-Opening Alters the Interaction Between the Salience Network and the Default-Mode Network显示文摘Dear Editor,Brain networks consist of several long-distance regions that interact constantly with each other.Interactions between networks suggest that different brain functions are coordinated during complex cognitive tasks[1].A triple-network mechanism underlying cognitive control has been found across task paradigms and stimulus modalities[2].This triple-network mechanism includes the interactions among the salience network(SN),the default mode network(DMN),and the central executive network(CEN). | Junrong Han Xuehai Wu Hang Wu Dong Wang Xuan She Musi Xie Fang Zhang Delong Zhang Xilin Zhang Pengmin Qin | 2020 | Neuroscience Bulletin2020，36，12： | 1 |
| 7 | Side population cells from HXO-Rb44 retinoblastoma cell line have cancer-initiating property显示文摘AIM:To ascertain whether side population(SP) cells in HXO-Rb44 retinoblastoma cell line have cancer stem cell-like property in vitro and in vivo.METHODS:We analyzed and sorted SP from HXO-Rb44 retinoblastoma cell line by Hoechst 33342 staining on flow cytometry.SP and NSP cells were determined their ability of proliferation and self-renewal by SP reanalysis,soft agar assay and tumor sphere assay in vitro.Clone formation was detected by seeding HXO-Rb44 and HXO-Rb44-RFP cells into soft agar.The expression of ABCG2,MDRI,Bmi-1 and Oct-4 was determined by RT-PCR between SP and non-SP(NSP) cells.Moreover,they were injected into nude mice to determine their tumorigency in vivo.RESULTS:SP from HXO-Rb44 retinoblastoma cell line could grow clonally in soft agar assays and form tumor spheres from single cells in conditioned media.The expressions of ABCG2,MDRI,Bmi-1 and Oct-4 were significantly higher in SP than NSP cells.As few as SP cells resulted in tumor formation in 6 of 12 injected sites,however,the injection of NSP cells failed to form new tumor.CONCLUSION:SP cells isolated by Hoechst 33342 from the HXO-Rb44 retinoblastoma cell line had property of high tumorigency in vivo and in vitro.Therefore,SP might be a target while developing retinoblastoma therapies. | Jun-Jun She, Zi-Ming Wang | 2011 | International Journal of Ophthalmology(English edition)2011，4，5： | 0 |
| 8 | Near-Infrared Light-Responsive Nitric Oxide Delivery Platform for Enhanced Radioimmunotherapy显示文摘Radiotherapy(RT)is a widely used way for cancer treatment.However,the efficiency of RT may come with various challenges such as low specificity,limitation by resistance,high dose and so on.Nitric oxide(NO)is known a very effective radiosensitizer of hypoxic tumor.However,NO cannot circulate in body with high concentration.Herein,an NIR light-responsive NO delivery system is developed for controlled and precisely release of NO to hypoxic tumors during radiotherapy.Tert-Butyl nitrite,which is an efficient NO source,is coupled to Ag2S quantum dots(QDs).NO could be generated and released from the Ag2S QDs effectively under the NIR irradiation due to the thermal effect.In addition,Ag is also a type of heavy metal that can benefit the RT therapy.We demonstrate that Ag2S NO delivery platforms remarkably maximize radiotherapy effects to inhibit tumor growth in CT26 tumor model.Furthermore,immunosuppressive tumor microenvironment is improved by our NO delivery system,significantly enhancing the anti-PD-L1 immune checkpoint blockade therapy.100% survival rate is achieved by the radio-immune combined therapy strategy based on the Ag2S NO delivery platforms.Our results suggest the promise of Ag2S NO delivery platforms for multifunctional cancer radioimmunotherapy. | Xuanfang Zhou Zhouqi Meng Jialin She Yaojia Zhang Xuan Yi Hailin Zhou Jing Zhong Ziliang Dong Xiao Han Muchao Chen Qin Fan Kai Yang Chao Wang | 2020 | Nano-Micro Letters2020，12，8： | 0 |
| 9 | Precipitation of High Mg-Calcite and Protodolomite Using Dead Biomass of Aerobic Halophilic Bacteria显示文摘The microbial dolomite model has been used to interpret the origin of sedimentary dolomite.In this model,the formation of low-temperature protodolomite,an important precursor to sedimentary dolomite,can be facilitated either by actively metabolizing cells of anaerobic microbes and aerobic halophilic archaea or by their inactive biomass.Aerobic halophilic bacteria are widely distributed in(proto-)dolomite-depositing evaporitic environments and their biomass might serve as a template for the crystallization of protodolomite.To test this hypothesis,carbonation experiments were conducted using dead biomass of an aerobic halophilic bacterium(Exiguobacterium sp.strain JBHLT-3).Our results show that dead biomass of JBHLT-3 can accelerate Mg2+uptake in carbonate mineral precipitates.In addition,the amount of Mg incorporated into Ca-Mg carbonates is proportional to the concentration of biomass.High Mg-calcite is produced with 0.25 or 0.5 g/L biomass,whereas protodolomite forms with 1 g/L biomass.This is confirmed by the main Raman peak of Ca-Mg carbonates,which shifts towards higher wavenumbers with increased Mg substitution.Microbial cells and their imprints are preserved on the surface of high Mg-calcite and protodolomite.Hence,this study furthers our understanding of the dolomitization within buried and dead microbial mats,which provides useful insights into the origin of ancient dolomite. | Qigao Fan Deng Liu Dominic Papineau Xuan Qiu Hongmei Wang Zhenbing She Linduo Zhao | 2023 | Journal of Earth Science2023，34，2： | 0 |
| 10 | Peripheral arterial filling time and peripheral retina fluorescence features in ultra-widefield angiography显示文摘AIM:To evaluate the peripheral arterial filling time(PAFT)and venous filling time(VFT)in eyes without known diseases that may influence filling process using ultra-widefield(UWF)fluorescein angiography(FA),and to review the peripheral retina fluorescence features.METHODS:A total of 30 eyes of 30 patients were retrospectively reviewed in this observational study.UWFFA was performed using Optos 200Tx.PAFT and VFT was recorded.The interval between the arterial or venous filling completion and the previous photo was documented.The appearance of the far peripheral retina was described as either granular background fluorescence or mottled fluorescent band or vascular leakage.Terminal vascular patterns was described as loop pattern or branching pattern.Microvascular abnormalities such as arteriovenous shunting,vessels crossing the horizontal raphe,right angle vessels,terminal networks,capillary nonperfusion,drusen or microaneurysms were evaluated.RESULTS:The normal limits of PAFT was 3.397-8.984s and 4.399-11.753s for VFT.The appearance of the far peripheral retina,defined as granular background(63%),mottled fluorescence(20%),or vascular leakage(17%),was symmetrical between both eyes.Capillary nonperfusion(23%)and microaneurysms(40%)were more frequently found in eyes with loop pattern than in eyes with branching pattern.Other peripheral signs such as right-angle vessels(73%),and terminal networks(80%)were commonly seen on UWF-FA in the normal peripheral retina.CONCLUSION:The main courses of retinal artery and vein filling time are overlapping with each other on UWF-FA.Notably,the arterial filling process is completed in the arteriovenous phase rather than the traditionally named arterial phase.There are various manifestations in the peripheral retina of normal eyes. | Hai-Cheng She Xi-Fang Zhang Yong-Peng Zhang Xuan Jiao Hai-Ying Zhou | 2021 | International Journal of Ophthalmology(English edition)2021，14，7： | 0 |
| 11 | Crystal structure and catalytic mechanism of the MbnBC holoenzyme required for methanobactin biosynthesis显示文摘Methanobactins(Mbns)are a family of copper binding peptides involved in copper uptake by methanotrophs,and are potential therapeutic agents for treating diseases characterized by disordered copper accumulation.Mbns are produced via modification of MbnA precursor peptides at cysteine residues catalyzed by the core biosynthetic machinery containing MbnB,an iron-dependent enzyme,and MbnC.However,mechanistic details underlying the catalysis of the MbnBC holoenzyme remain unclear.Here,we present crystal structures of MbnABC complexes from two distinct species,revealing that the leader peptide of the substrate MbnA binds MbnC for recruitment of the MbnBC holoenzyme,while the core peptide of MbnA resides in the catalytic cavity created by the MbnB-MbnC interaction which harbors a unique tri-iron cluster.Ligation of the substrate sulfhydryl group to the tri-iron center achieves a dioxygen-dependent reaction for oxazolone thioamide installation.Structural analysis of the MbnABC complexes together with functional investigation of MbnB variants identifed a conserved catalytic aspartate residue as a general base required for MbnBC-mediated MbnA modifcation.Together,our study reveals the similar architecture and function of MbnBC complexes from different species,demonstrating an evolutionarily conserved catalytic mechanism of the MbnBC holoenzymes. | Chao Dou Zhaolin Long Shoujie Li Dan Zhou Ying Jin Li Zhang Xuan Zhang Yanhui Zheng Lin Li Xiaofeng Zhu Zheng Liu Siyu He Weizhu Yan Lulu Yang Jie Xiong Xianghui Fu Shiqian Qi Haiyan Ren She Chen Lunzhi Dai Binju Wang Wei Cheng | 2022 | Cell Research2022，32，3： | 0 |
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