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| 1 | Physiological and molecular studies of staygreen caused by pod removal and seed injury in soybean显示文摘Leaves provide substances and signals for pod and seed development in soybean.However,the regulatory feedbacks of pod and seed to leaf development remain unclear.We investigated the effects of pod and seed on leaf senescence by conducting pod removal and seed injury experiments.Pod removal and seed injury delayed leaf senescence and caused the staygreen phenotype of leaves.There were dosage effects of pod number on the extent of staygreen in depodded plants.The concentrations of chlorophyll(SPAD value,an index of relative chlorophyll content),soluble protein,and soluble sugar in the leaves of depodded plants were higher than those of intact plants.During seed development,the content of IAA decreased,while that of ABA increased.This trend was more pronounced in intact than in depodded and seed-injured plants.The GA3/ABA ratio decreased gradually in all treatments.The content of GA3 was relatively stable and was higher in intact than in depodded plants.The expression levels of four senescence-related genes,Gm SARK,Gm SGR1,Gm CYN1,and Gm NAC,declined in depodded or seed-injured treatments and were positively correlated with the number of leaves retained on plants.Gm FT2 a,the major flowering-promoting gene,was expressed at a higher level while E1,a key flowering inhibitory gene,was expressed at a lower level in depodded than in intact plants.We propose that the pod or seed can regulate leaf development.When the seed is aborted owing to disease infection or pest attack,the leaves stay green because of the absence of the seed signals for senescence. | Xinxin Zhang Min Wang Tingting Wu Cunxiang Wu Bingjun Jiang Changhong Guo Tianfu Han | 2016 | The Crop Journal2016，4，6： | 23 |
| 2 | Hairy Leaf 6, an AP2/ERF Transcription Factor, Interacts with OsWOX3B and Regulates Trichome Formation in Rice显示文摘毛状体形成广泛地在植物为表皮的房间区别和房间形态发生作为一个机械学的模型被学习了。然而,位于毛状体形成下面的基因、分子的机制(即,开始和延伸) 在米饭仍然保持大部分不清楚。这里,我们报导一个 AP2/ERF 抄写因素,毛乎乎的叶 6 (HL6 ) ,它在米饭控制毛状体形成。功能的分析表明 HL6 transcriptionally 在米饭调整毛状体延伸,它依赖于功能的 OsWOX3B,在毛状体充当一个关键管理者的包含 homeodomain 蛋白质开始。生物化学、分子的基因分析证明那 HL6 身体上交往与 OsWOX3B,和他们俩在毛状体形成, OsWOX3B 多半与它的直接目标基因之一在提高 HL6 的有约束力的能力期间调整一些植物生长素相关的基因的表达式, OsYUCCA5。人口基因分析显示 HL6 在米饭驯服期间在否定选择下面。一起拿,我们的调查结果提供新卓见进毛状体形成的分子的规章的网络在 ? 米饭。 | Wenqiang Sun Dawei Gao Yin Xiong Xinxin Tang Xiongfeng Xiao Chongrong Wang Sibin Yu | 2017 | Molecular Plant2017，10，11： | 20 |
| 3 | Ferroptosis is essential for diabetic cardiomyopathy and is prevented by sulforaphane via AMPK/NRF2 pathways显示文摘Herein,we define the role of ferroptosis in the pathogenesis of diabetic cardiomyopathy(DCM)by examining the expression of key regulators of ferroptosis in mice with DCM and a new ex vivo DCM model.Advanced glycation end-products(AGEs),an important pathogenic factor of DCM,were found to induce ferroptosis in engineered cardiac tissues(ECTs),as reflected through increased levels of Ptgs2 and lipid peroxides and decreased ferritin and SLC7 A11 levels.Typical morphological changes of ferroptosis in cardiomyocytes were observed using transmission electron microscopy.Inhibition of ferroptosis with ferrostatin-1 and deferoxamine prevented AGE-induced ECT remodeling and dysfunction.Ferroptosis was also evidenced in the heart of type 2 diabetic mice with DCM.Inhibition of ferroptosis by liproxstatin-1 prevented the development of diastolic dysfunction at 3 months after the onset of diabetes.Nuclear factor erythroid 2-related factor 2(NRF2)activated by sulforaphane inhibited cardiac cell ferroptosis in both AGE-treated ECTs and hearts of DCM mice by upregulating ferritin and SLC7 A11 levels.The protective effect of sulforaphane on ferroptosis was AMP-activated protein kinase(AMPK)-dependent.These findings suggest that ferroptosis plays an essential role in the pathogenesis of DCM;sulforaphane prevents ferroptosis and associated pathogenesis via AMPK-mediated NRF2 activation.This suggests a feasible therapeutic approach with sulforaphane to clinically prevent ferroptosis and DCM. | Xiang Wang Xinxin Chen Wenqian Zhou Hongbo Men Terigen Bao Yike Sun Quanwei Wang Yi Tan Bradley B.Keller Qian Tong Yang Zheng Lu Cai | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 20 |
| 4 | Distribution of organic carbon fractions in soil aggregates in Chinese fir plantations with different stand ages显示文摘Background:Revealing the variations in soil aggregate-related organic carbon(OC)and labile organic carbon(LOC)fractions in a chronosequence of Chinese fir plantations plays an important role in better understanding the impact of soil carbon sink or source on the Chinese fir plantation ecosystem.In this study,soil samples in a depth of 0–20 cm were collected from Chinese fir plantations at different stand ages(0,9,17,and 26 years old)in Guangxi,China.With the optimal moisture sieving method adopted,the soil aggregates of 4 different sizes were obtained,including>2-mm,2–1-mm,1–0.25-mm,and<0.25-mm aggregates.Soil OC and LOC fractions were measured in the aggregates of different sizes.The LOC fractions included readily oxidizable carbon(ROC),particulate organic carbon(POC),microbial biomass carbon(MBC),water-soluble organic carbon(WOC),and mineralized organic carbon(MOC).Results:Soil aggregate stability,as indicated by the mean weight diameter(MWD),was the highest in the 17-yearold Chinese fir plantations and was significantly positively related(p<0.05)to the concentrations of OC and LOC fractions(except for the ROC and MOC),with the POC in particular.As for all stand ages of Chinese fir plantations,the concentrations of soil OC and LOC fractions were significantly increased as the aggregate size decreased.Consequently,there were more OC and LOC fractions distributed in the<0.25-mm aggregates.During the stand development,the concentrations of soil OC and LOC fractions first increased and then decreased,with the highest levels detected in the 17-year-old Chinese fir plantations,indicating that the 17-year-old Chinese fir plantations were conducive to the accumulation of soil OC and LOC fractions.Conclusion:After 17 years of planting,promoted soil carbon(especially for the POC)accumulation contributes significantly to enhancing soil aggregate stability for the Chinese fir plantations in Guangxi,China. | Xinxin He Yongzhen Huang Qianchun Zhang Shaoming Ye Shengqiang Wang | 2021 | Ecological Processes2021，10，1： | 11 |
| 5 | Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches显示文摘The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isolates collected from six culture media.The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp.,Pseudomonas sp.,Aeromonas sp.and Acinetobacter sp.Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets.While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample.Sequence analysis revealed that the highest proportion (14.7%) of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis,which is used to remove phosphorous from wastewater.Our results indicated that combining different approaches can produce complementary information,thus generate a more accurate view of microbial community in bulking sludge. | Decai Jin Ping Wang Zhihui Bai Xinxin Wang Hong Peng Rong Qi Zhisheng Yu Guoqiang Zhuang | 2011 | Journal of Environmental Sciences2011，23，11： | 11 |
| 6 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 7 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 8 | Estimation of reproduction numbers of COVID-19 in typical countries and epidemic trends under different prevention and control scenarios显示文摘The coronavirus disease 2019(COVID-19)has become a life-threatening pandemic.The epidemic trends in different countries vary considerably due to different policy-making and resources mobilization.We calculated basic reproduction number(R0)and the time-varying estimate of the effective reproductive number(Rt)of COVID-19 by using the maximum likelihood method and the sequential Bayesian method,respectively.European and North American countries possessed higher (R0)and unsteady Rt fluctuations,whereas some heavily affected Asian countries showed relatively low (R0)and declining Rt now.The numbers of patients in Africa and Latin America are still low,but the potential risk of huge outbreaks cannot be ignored.Three scenarios were then simulated,generating distinct outcomes by using SEIR(susceptible,exposed,infectious,and removed)model.First,evidence-based prompt responses yield lower transmission rate followed by decreasing Rt.Second,implementation of effective control policies at a relatively late stage,in spite of huge casualties at early phase,can still achieve containment and mitigation.Third,wisely taking advantage of the time-window for developing countries in Africa and Latin America to adopt adequate measures can save more people’s life.Our mathematical modeling provides evidence for international communities to develop sound design of containment and mitigation policies for COVID-19. | Chen Xu Yinqiao Dong Xiaoyue Yu Huwen Wang Lhakpa Tsamlag Shuxian Zhang Ruijie Chang Zezhou Wang Yuelin Yu Rusi Long Ying Wang Gang Xu Tian Shen Suping Wang Xinxin Zhang Hui Wang Yong Cai | 2020 | Frontiers of Medicine2020，14，5： | 8 |
| 9 | Archaeal community structure along a gradient of petroleum contamination in saline-alkali soil显示文摘The response of archaeal communities to petroleum contamination in saline-alkali soil was characterized by analyses of three soil samples with different total petroleum hydrocarbon concentrations.Through the construction and screening of 16S rRNA gene clone libraries based on DNA extracts from these soils,nine distinct phylogenetic groups were identified.Statistical analyses showed that the distribution of archaeal community structures differ significantly along the gradient of petroleum contamination in these three saline- alkali soils.Five phylogenetic groups were dominant in the control soil,two of which were also abundant in the lightly contaminated soil.Four phylogenetic groups were dominant in heavily contaminated soil,one of which was also abundant in the lightly contaminated soil.The halophilic genus of Haloferax and the haloalkaliphilic genus of Natronomonas were more abundant in heavily contaminated soil.These results suggested that the genera of Haloferax and Natronomonas may have a role in the natural attenuation of petroleum- contaminated saline-alkali soil. | Xinxin Wang Zhen Han Zhihui Bai Jingchun Tang Anzhou Ma Jizheng He Guoqiang Zhuang | 2011 | Journal of Environmental Sciences2011，23，11： | 8 |
| 10 | Molecular characteristics of the omp A gene of serotype B Chlamydia trachomatis in Qinghai Tibetan primary school students显示文摘To study the molecular characteristics of Chlamydia trachomatis, the major outer membrane protein gene(omp A) of C. trachomatis from primary school students with trachoma residing in the Qinghai Tibetan area was sequenced and compared with the same serotype in Gen Bank. In Jianshetang Primary School and Galeng Central Primary School in the Galeng Tibetan Township of Qinghai Haidong Sala Autonomous County, scraped samples were collected from the upper tarsal conjunctiva and lower conjunctival sac of both eyes of 45 students with trachoma, stored at 4°C, and transported to Beijing Tongren Hospital by air within 24 h. The samples were screened for C. trachomatis by real-time PCR. The omp A gene from the C. trachomatis-positive samples was amplified by nested PCR. The serotype was confirmed by National Center for Biotechnology Information(NCBI) BLAST search and homology analysis. The entire omp A gene sequence was compared with the corresponding gene sequences of serotype B strains available in Gen Bank. Of the 45 students aged 6–13 years with trachoma, 26 C. trachomatis-positive students were identified by the initial real-time PCR screening(average age,(9.09±1.63) years; sex ratio, 1.0), accounting for 57.78%(26/45). The cycle threshold values for real-time PCR were 16.79–37.77. Half(13/26) of C. trachomatis-positive students had a bacterial copy number of >105. The compliance rate of the omp A gene sequences with the C. trachomatis serotype B strains in Gen Bank was up to 99%. Two novel genetic mutations were found when the omp A gene was compared with those of the 11 serotype B strains in Gen Bank. The two non-synonymous mutations were located at(i) position 271 in the second constant domain, an adenine(A) to guanine(G) substitution(ACT?GCT), changing the amino acid at position 91 from threonine to alanine(Thr?Ala) in all 26 strains; and(ii) position 887 in the fourth variable domain, a cytosine(C) to thymine(T) substitution(GCA?GTA), changing the amino acid at residue 296 from alanine to valine(Ala?Val) in four of the 26 strains. Six mutations were identified relative to ATCC VR-573. The strains could be divided into two gene clusters according to the mutation at nucleotide position 887: CQZ-1(China Qinghai Tibetan-1) and CQZ-2(China Qinghai Tibetan-2). We thus detected two novel serotype B mutant strains of C. trachomatis among study subjects with trachoma. | Xue Li Shaoya Zhang Qingfeng Liang Mei Wang Ailian Hu Xiuyuan Li Benshan Yang Mingxin Zhang Ningli Wang Xinxin Lu | 2016 | Science China(Life Sciences)2016，59，6： | 6 |
| 11 | Population-level economic burden of lung cancer in China:Provisional prevalence-based estimations,2017-2030显示文摘Objective:Population-level economic burden is essential for prioritizing healthcare resources and healthcare budget making in the future.However,little is known about the economic burden of lung cancer in China.Methods:A prevalence-based approach was adopted to estimate the economic burden of lung cancer,including direct expenditure(medical and non-medical)and indirect cost(disability and premature death).Data on direct expenditure and work-loss days per patient in each year post-diagnosis were obtained from two primary surveys.Other parameters were obtained from literatures and official reports.Projections were conducted based on varying parameters.All expenditure data were reported in United States dollars(USD)using 2017 value(exchange rate:1 USD=6.760 CNY),with the discount rate of 3%.Results:The total economic burden of lung cancer was estimated to be 25,069 million USD in China in 2017(0.121%of gross domestic productivity,GDP).The estimated direct expenditure was 11,098 million USD,up to1.43%of total healthcare expenditure for China,covering 10,303 million USD and 795 million USD for medical and non-medical expenditure,respectively.The estimated indirect cost was 13,971 million,including 1,517 million USD due to disability and 12,454 million USD due to premature death.Under current assumptions,the projected total economic burden would increase to 30.1 billion USD,40.4 billion USD,and 53.4 billion USD in 2020,2025,and 2030,accounting for 0.121%,0.131%,and 0.146%of China's GDP,respectively.However,if China meets the United Nation sustainable development goal of reducing premature death from non-communicable diseases by one-third by 2030,the total economic burden in 2030 would be 31.9 billion USD,0.087%of China's GDP.Conclusions:The economic burden of lung cancer in China in 2017 is substantial and more likely to increase significantly in the future.Policy makers need to take urgent actions in budget making for health systems.The economic burden could be alleviated by reducing the disease burden of lung cancer via effective control and prevention actions. | Chengcheng Liu Jufang Shi Hong Wang Xinxin Yan Le Wang Jiansong Ren Mark Parascandola Wanqing Chen Alin Dai | 2021 | Chinese Journal of Cancer Research2021，33，1： | 6 |
| 12 | High-resolution carbon isotope record for the Paleocene-Eocene thermal maximum from the Nanyang Basin,Central China显示文摘古新始新世的热最大值(PETM ) 是全球温暖的一个短暂事件,与发生在古新 / 始新世的边界的巨大的大气的温室气体输入联系了。Biostratigraphic 和同位素 stratigraphic 研究显示 PETM 事件很好在 Nanyang 盆在 Yuhuangding 节的泥土存款被记录,华中,与碳同位素,在 19-m-thick 泥土以内的锝 ?.1 鈥 ? 的否定旅行扔。这是到目前为止在世界上发现的 PETM 的最高的分辨率记录。PETM 事件在 2-cm-thick 泥土沉积以内被触发,与未 1 3C (稳定的碳同位素比率) 从的减少 ? 3.2 鈥 ? 到 ? 5.2 鈥吗?为是的一个短暂时期建议一个巨大的甲烷水合物版本可能由一个灾难的事件引起了。在海洋、陆上的记录之间的比较为 PETM 事件显示鈥淭h ree 阶段模型鈥 ? 。开始在未 1 3C 记录有一次快速的否定旅行,由一个慢慢地减少的趋势,然后渐渐的积极恢复列在后面,相应分别地到海洋的甲烷水合物的快速的分离,由甲烷然后释放甲烷的消费的一个慢版本列在后面。 | ZHU Min DINGZhongLi WANG Xu CHEN ZuoLing JIANG HanChao DONG XinXin JI JunLiang TANG ZiHua LUO Pan | 2010 | Chinese Science Bulletin2010，55，31： | 6 |
| 13 | The spatial pattern of farming and factors influencing it during the Peiligang culture period in the middle Yellow River valley, China显示文摘The Peiligang culture(ca.7000–5000 cal BC)is the earliest Neolithic culture in the middle Yellow River valley and represents the emergence of cereal farming in the region[1].It has long been considered representative of millet farming[2],which requires a dry field,a certain amount of rainfall,and minimal crop management,but new records reveal that mixed farming of millet and rice(Oryza sp.)took place during this period[3].Therefore,it is possible | Can Wang Houyuan Lu Wanfa Gu Naiqin Wu Jianping Zhang Xinxin Zuo Fengjiang Li Daojing Wang Yajie Dong Songzhi Wang Yanfeng Liu Yingjian Bao Yayi Hu | 2017 | Science Bulletin2017，62，23： | 6 |
| 14 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
| 15 | Distribution and contamination of metals and biogenic elements in sediments from Zhifu Bay of the Yellow Sea,China显示文摘Metals and biogenic elements were analyzed from surface sediments of sixteen stations and a 32 cm core collected from Zhifu Bay in July 2009.High concentrations of biogenic silica(BSi)reflected the high diatom productivity in the Bay.Meanwhile,total organic carbon(TOC)in Zhifu Bay was mainly from terrestrial sources.All metals were lower than the marine sediment quality guidelines.Based on the vertical profiles of biogenic elements,there was a clear increase of TOC,total nitrogen(TN),and total phosphate(TP)between1985 and 2000.TN concentrations decreased after the start of the operation of the sewage treatment plant in 1998;however TOC and BSi remained at high levels.Metal concentrations showed a general increase from the bottom to the top of the core.Most anthropogenic metals reached a maximum during 1996 and 2000,and decreased after the startup of the sewage plant;however,they have increased again recently.The results from correlation analysis and principal component analysis show that industrial pollution is the main source of Cd,Cr,Cu,Ni,Pb and Zn contamination in Zhifu Bay,and Yantai Port and ship transportation also contribute a lot to Cd,Cu,Hg,and Pb pollution. | Zhaohui Wang Xinxin Lu Ke Zhang | 2016 | Journal of Environmental Sciences2016，28，3： | 6 |
| 16 | Whole-genome resequencing of Osmanthus fragrans provides insights into flower color evolution显示文摘Osmanthus fragrans is a well-known ornamental plant that has been domesticated in China for 2500 years.More than 160 cultivars have been found during this long period of domestication,and they have subsequently been divided into four cultivar groups,including the Yingui,Jingui,Dangui,and Sijigui groups.These groups provide a set of materials to study genetic evolution and variability.Here,we constructed a reference genome of O.fragrans‘Liuyejingui’in the Jingui group and investigated its floral color traits and domestication history by resequencing a total of 122 samples,including 119 O.fragrans accessions and three other Osmanthus species,at an average sequencing depth of 15×.The population structure analysis showed that these 119 accessions formed an apparent regional cluster.The results of linkage disequilibrium(LD)decay analysis suggested that varieties with orange/red flower color in the Dangui group had undergone more artificial directional selection;these varieties had the highest LD values among the four groups,followed by the Sijigui,Jingui,and Yingui groups.Through a genome-wide association study,we further identified significant quantitative trait loci and genomic regions containing several genes,such as ethylene-responsive transcription factor 2 and Arabidopsis pseudoresponse regulator 2,that are positively associated with petal color.Moreover,we found a frameshift mutation with a 34-bp deletion in the first coding region of the carotenoid cleavage dioxygenase 4 gene.This frameshift mutation existed in at least one site on both alleles in all varieties of the Dangui group.The results from this study shed light on the genetic basis of domestication in woody plants,such as O.fragrans. | Hongguo Chen Xiangling Zeng Jie Yang Xuan Cai Yumin Shi Riru Zheng Zhenqi Wang Junyi Liu Xinxin Yi Siwei Xiao Qiang Fu Jingjing Zou Caiyun Wang | 2021 | Horticulture Research2021，8，1： | 5 |
| 17 | NIR light-induced tumor phototherapy using ICG delivery system based on platelet-membrane-camouflaged hollow bismuth selenide nanoparticles显示文摘Near-infrared(NIR)light-triggered photothermal therapy(PTT)is a promising treatment strategy for treating cancer.The combination of nanotechnology and NIR has been widely applied.However,the therapeutic efficacy of the drug-delivery system depends on their ability to avoid phagocytosis of endothelial system,cross the biological barriers,prolong circulation life,localize and rapidly release the therapeutic at target sites.In this work,we designed a platelet membrane(PM)-camouflaged hollow mesoporous bismuth selenide nanoparticles(BS NPs)loading with indocyanine green(ICG)(PM@BS-ICG NPs)to achieve the above advantages.PM-coating has active tumor-targe ting ability which could preve nt drug leakage and provide drug long circulation,causing drug delivery systems to accumulate in tumor sites effectively.Moreover,as a type of the photothermal sensitizers,BS NPs are used as the inner cores to improve ICG stability and are served as scaffolds to enhance the hardness of this drug delivery system.For one hand,the thermal vibration of BS NPs under NIR laser irradiation causes tumor inhibition through hyperthermia.For another hand,this hyperthermia process could damage PM and let ICG rapid release from PM@BS-ICG NPs.The in vitro and in vivo results showed that this biomimetic nano-drug delivery system exhibits obvious antitumor activity which has good application prospect. | Kaili Ding Cuixia Zheng Lingling Sun Xinxin Liu Yanyan Yin Lei Wang | 2020 | Chinese Chemical Letters2020，31，5： | 5 |
| 18 | Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice显示文摘The lateral hypothalamic area(LHA)plays a pivotal role in regulating consciousness transition,in which orexinergic neurons,GABAergic neurons,and melanin-concentrating hormone neurons are involved.Glutamatergic neurons have a large population in the LHA,but their anesthesia-related effect has not been explored.Here,we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice.In contrast,chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery.Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram(EEG)and shifted EEG features to an arousal pattern.Photostimulation of LHA glutamatergic projections to the lateral habenula(LHb)also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level.Collectively,LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features. | Shiyi Zhao Rui Li Huiming Li Sa Wang Xinxin Zhang Dan Wang Juan Guo Huihui Li Ao Li Tingting Tong Haixing Zhong Qianzi Yang Hailong Dong | 2021 | Neuroscience Bulletin2021，37，7： | 5 |
| 19 | A MEMS Device for in-situ TEM Test of SCS Nanobeam显示文摘In-situ tensile testing in TEM (transmission electron microscopy) is a useful tool for studying mechanical properties of nano-structures because it can provide quanti-tative information on sample deformation at atomic scale. To facilitate in-situ TEM tensile tests of SCS (single crystal silicon) nanobeam, a MEMS tensile-testing chip was designed and fabricated. The chip was fabricated by means of bulk micro-machining and wafer bonding techniques. An SCS nanobeam, a comb drive ac-tuator, a force sensor beam and an electron beam window were integrated into the chip. With the on-chip comb-drive-actuator stretching the nanobeam and in-situ TEM observation, tensile test on a 90 nm-thick nanobeam was performed and the strain-stress relationship was obtained. The Young's modulus was fitted to be 161 GPa and did not show the size effect. | JIN QinHua WANG YueLin LI Tie LI XinXin XU FangFang | 2008 | Science China(Technological Sciences)2008，51，9： | 5 |
| 20 | Mandible exosomal ssc-mir-133b regulates tooth development in miniature swine via endogenous apoptosis显示文摘Signal transduction between different organs is crucial in the normal development of the human body. As an important medium for signal communication, exosomes can transfer important information, such as microRNAs(miRNAs), from donors to receptors.MiRNAs are known to fine-tune a variety of biological processes, including maxillofacial development; however, the underlying mechanism remains largely unknown. In the present study, transient apoptosis was found to be due to the expression of a miniature swine maxillofacial-specific miRNA, ssc-mir-133b. Upregulation of ssc-mir-133b resulted in robust apoptosis in primary dental mesenchymal cells in the maxillofacial region. Cell leukemia myeloid 1(Mcl-1) was verified as the functional target, which triggered further downstream activation of endogenous mitochondria-related apoptotic processes during tooth development.More importantly, mandible exosomes were responsible for the initial apoptosis signal. An animal study demonstrated that ectopic expression of ssc-mir-133 b resulted in failed tooth formation after 12 weeks of subcutaneous transplantation in nude mice. The tooth germ developed abnormally without the indispensable exosomal signals from the mandible. | Ye Li Xinxin Wang Jiali Ren Xiaoshan Wu Guoqing Li Zhipeng Fan Chunmei Zhang Ang Li Songlin Wang | 2018 | Bone Research2018，6，4： | 5 |
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