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| 1 | Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis显示文摘AIM: To investigate the intestinal anti-inflammatory effect and mechanism of a probiotic Lactobacillus sakuvarius ssp.salivariusCECT5713 in the TNBS model of rat colitis.METHODS: Female Wistar rats (180-200 g) were used in this study. A group of rats were administered orally the probiotic L. salivarius ssp. salivarius (5×108 CFU suspended in 0.5 mL of skimmed milk) daily for 3 wk. Two additional groups were used for reference, a non-colitic and a control colitic without probiotic treatment, which received orally the vehicle used to administer the probiotic. Two weeks after starting the experiment, the rats were rendered colitic by intracolonic administration of 10 mg of TNBS dissolved in 0.25 mL of 500 mL/L ethanol. One week after colitis induction, all animals were killed and colonic damage was evaluated both histologically and biochemically. The biochemical studies performed in colonic homogenates include determination of myeloperoxidase (MPO) activity,glutathione (GSH) content, leukotriene B4 (LTB4) and tumor necrosis factor α (TNF-α) levels, as well as inducible nitric oxide synthase (iNOS) expression. In addition, the luminal contents obtained from colonic samples were used for microbiological studies, in order to determine Lactobacilli and Bifidobacteria counts.RESULTS: Treatment of colitic rats with L. salivarius ssp.salivarius resulted in amelioration of the inflammatory response in colitic rats, when compared with the corresponding control group without probiotic treatment. This antiinflammatory effect was evidenced macroscopically by a significant reduction in the extent of colonic necrosis and/or inflammation induced by the administration of TNBS/ethanol (2.3+0.4 cm vs3.4+0.3 cm in control group,P<0.01) and histologically by improvement of the colonic architecture associated with a reduction in the neutrophil infiltrate in comparison with non-treated colitic rats. The latter was confirmed biochemically by a significant reduction of colonic MPO activity (105.3+26.0 U/g vs180.6±21.9 U/g, P<0.05), a marker of neutrophil infiltration. The beneficial effect was associated with an increase of the colonic GSH content (1 252±42 nmol/g vs 1 087+51 nmol/g, P<0.05),which is depleted in colitic rats, as a consequence of the oxidative stress induced by the inflammatory process. In addition, the treatment of colitic rats with L. salivarius resulted in a significant reduction of colonic TNF-α levels (509.4±68.2 pg/g vs782.9±60.1 pglg, P<0.01) and in a lower colonic iNOS expression, when compared to TNBS control animals without probiotic administration. Finally,treated colitic rats showed higher counts of Lactobacilli species in colonic contents than control colitic rats, whereas no differences were observed in Bifidobacteria counts.CONCLUSION: Administration of the probiotic L. salivarius ssp. salivarius CECT5713 facilitates the recovery of the inflamed tissue in the TNBS model of rat colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response in the intestine, such as cytokines, including TNF-α and NO. This beneficial effect could be ascribed to its effect on the altered immune response that occurs in this inflammatory condition. | Laura Peran Desiree Camuesco Monica Comalada Ana Nieto Angel Concha Maria Paz Diaz-Ropero Monica Olivares Jordi Xaus Antonio Zarzuelo Julio Galvez | 2005 | World Journal of Gastroenterology2005，11，33： | 17 |
| 2 | Decorin inhibits macrophage colony-stimulating factor proliferation of macrophages and enhances cell survival through induction of p27 (Kipl) and p21 (Waf1)显示文摘 | Xaus J Comalada M Card ó M | | 0，，07： | 1 |
| 3 | Interferon gamma induces the expression of p21waf-1 and arrests macrophage cell cycle,preventing induction of apoptosis显示文摘 | Xaus J Cardo M Valledor AF | 1999 | Immunity1999，11，： | 1 |
| 4 | Effect of ozone treatment on reactive oxygen species and adenosine production during hepatic ischemia-reperfusion显示文摘 | Peralta C Xaus C Barttrons R | 2000 | J Free Radic Res2000，33，5： | 1 |
| 5 | Protective effect of ozone treatment on the injury associated with hepatic ischemia - reperfusion : antioxidant - prooxidant balance 显示文摘 | Peralta C Leen OS Xaus C | 1999 | Free Radie Res1999，31，3： | 1 |
| 6 | Influence of nitric oxide synthase and kinin antagonists on metabolic parameters in chronic strepozotocin induced diabetes mellitus显示文摘 | Gonzalez E Rosellocatafau J Xaus C | 1997 | Prostaglandins1997，53，5： | 1 |
| 7 | The expression of MHC classⅡ genes in macrophages is cell cycle dependent显示文摘 | Xaus J Commalada M Barrachina M | 2000 | J Immunol2000，165，11： | 1 |
| 8 | Ischemic preconditioning: a defense mechanism against the reactive oxygen species generated after hepatic ischemia reperfusion显示文摘 | Peralta C Bulbena O Xaus C | 2002 | Transplantation2002，73，8： | 1 |
| 9 | Intestinal and immunological effects of daily oral administration of Lactobacillus salivarius CECT5713 to healthy adults显示文摘 | Saleta Sierra Federico Lara-Villoslada Lluis Sempere Mónica Olivares Julio Boza Jordi Xaus | 2010 | Anaerobe2010，，3： | 1 |
| 10 | The balance between caseins and whey proteins in cow' s milk determines its aUergenicity 显示文摘 | Lara-Villoslada F Olivares M Xaus J | 2005 | Journal of Dairy Science2005，88，： | 1 |
| 11 | The balance between caseins and whey proteins in cow's milk determines its allergenicity 显示文摘 | LARA-VILLOSLADA F OLIVARES M XAUS J | 2005 | Dairy Sci2005，，88： | 1 |
| 12 | Hepatic preconditioning in rats is defined by a balance of adenosine and xanthine显示文摘 | Closa D Xaus C | 1998 | Hepatology1998，28，： | 1 |
| 13 | Hepatic preconditioning in rats is defined by a balance of adenosine and xanthine显示文摘 | Peralts C Closa D Xaus C | 1998 | Hepatology1998，28，3： | 1 |
| 14 | Protective effect of liver ischemic preconditioning on liver and lung injury induced By hepatic ischemia -reperfusion in the rat 显示文摘 | Peralta C Prats N Xaus C | 1999 | Hepatology1999，30，6： | 1 |
| 15 | Protective effect of liver ischemic preconditioning on liver and lung injury induced by hepatic ischemia - reperfusion in the rat显示文摘 | Peralta C Prats N Xaus C | 1999 | Hepatology1999，30，6： | 1 |
| 16 | Protective effect of ozone treatment on the injury associated with hepatic ischemia- reperfusion : Antioxidant-Prooxidant balance 显示文摘 | Peralta C Leon O S Xaus C | 1999 | Free Radic Res1999，31，： | 1 |
| 17 | Effect of ozone treatment on reactive oxygen species and adenosine production during hepatic ischemia-reperfusion显示文摘 | PERAITA C XAUS C BARTTRONS R | 2000 | Free Radic Res2000，33，5： | 1 |
| 18 | Effect of ozone treatment on reactive oxygen species and asenosine production during hepatic ischemia-reperfusion显示文摘 | Peralta C Xaus C Bartrons R Leon OS Gelpi E Rosello-Catafau J | 2000 | Free Radic Res2000，33，8： | 1 |
| 19 | The expression of MHC class II genes in macrophages is cell cycle dependent显示文摘 | Xaus J Commalada M Barrachina M | 2000 | J Immunol2000，165，11： | 1 |
| 20 | Protective effect of liver ischemic preconditioning on liver and lung injury induced by hepatic ischemia-reperfusion in the rat 显示文摘 | Peralta C Prats N Xaus C | 1999 | Hepatology1999，30，6： | 1 |
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