| 3 | Vaspin alleviates pathological cardiac hypertrophy by regulating autophagy-dependent myocardial senescence显示文摘Background:Visceral adipose tissue-derived serine protease inhibitor(vaspin),a secretory adipokine,protects against insulin resistance.Recent studies have demonstrated that serum vaspin levels are decreased in patients with coronary artery disease and that vaspin protects against myocardial ischemia-reperfusion injury and atherosclerosis.However,it remains unclear whether vaspin exerts specific effects on pathological cardiac hypertrophy.Methods:An in vivo study was conducted using a cardiac hypertrophy model established by subcutaneous injection of isoproterenol(ISO)in C57BL/6 and vaspin-ko mice.Rapamycin was administered intraperitoneally to mice,for further study.H9c2 cells and neonatal rat ventricular myocytes(NRVMs)were treated with ISO to induce hypertrophy.Human vaspin fusion protein,the proteasome inhibitor MG132,and chloroquine diphosphate were used for further mechanistic studies.Results:Here,we provide the first evidence that vaspin knockdown results in markedly exaggerated cardiac hypertrophy,fibrosis,and cardiomyocyte senescence in mice treated with ISO.Conversely,the administration of exogenous recombinant human vaspin protected NRVMs in vitro against ISO-induced hypertrophy and senescence.Furthermore,vaspin significantly potentiated the ISO-induced decrease in autophagy.Both rapamycin and chloroquine diphosphate regulated autophagy in vivo and in vitro,respectively,and participated in vaspin-mediated cardioprotection.Moreover,the PI3K-AKT-mTOR pathway plays a critical role in vaspin-mediated autophagy in cardiac tissues and NRVMs.Our data showed that vaspin downregulated the p85 and p110 subunits of PI3K by linking p85 and p110 to NEDD4L-mediated ubiquitination degradation.Conclusion:Our results show,for the first time,that vaspin functions as a critical regulator that alleviates pathological cardiac hypertrophy by regulating autophagy-dependent myocardial senescence,providing potential preventive and therapeutic targets for pathological cardiac hypertrophy. | Haiying Rui Huaxiang Yu Dan Zou Kai Chi Ping Xu Xiaoshuai Song Lulu Liu Xuting Wu Jinxin Wang Li Xue | 2024 | Emergency and Critical Care Medicine2024，4，1： | 0 |
| 4 | Effect of Cryopreservation on DNA Integrity and Morphological Structure of Boar Sperm显示文摘The paper was to explore the effect of cryopreservation on DNA integrity and morphological structure of boar sperm,and to explore the protective effect of trehalose and lactose on frozen boar sperm. The morphology,ultrastructure and DNA integrity of sperms were observed by phase contract microscope,scanning electron microscope( SEM) and acridine orange( AO) staining,respectively. The results showed that the normal morphological rate and DNA integrity rate of frozen sperms were significantly lower than that of fresh sperms( 95. 5% and 94. 7%,respectively),and the difference between two frozen groups was also significant( P <0. 05). The normal morphological rates in trehalose group and lactose group were 74. 7% and 67. 6%,while DNA integrity rates in trehalose group and lactose group were 66. 4% and 63. 2%,respectively. The common deformations of frozen sperms under SEM were partial or complete fracture between head and neck,swollen neck,damaged acrosome structure. | Yang Jianping Wu Caihong Zhao Xuting Zhou Chunbao Yao Jing Zhang Bin Zheng Xiaofeng | 2014 | Animal Husbandry and Feed Science2014，6，6： | 0 |
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