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| 1 | Mycoplasma infections and different human carcinomas显示文摘AIM To explore relationships between human carcinomas and mycoplasma infection.METHODS Monoclonal antibody PD4, which specifically recognizes a distinct protein from mycoplasma hyorhinis, was used to detect mycoplasma infection in different paraffinembedded carcinoma tissues with immunohistochemistry. PCR was applied to amplify the mycoplasma DNA from the positive samples for confirming immunohistochemistry.RESULTS Fifty of 90 cases (56%) of gastric carcinoma were positive for mycoplasma hyorhinis. In other gastric diseases, the mycoplasma infection ratio was 28% (18/49) in chronic superficial gastritis, 30% (14/ 46) in gastric ulcer and 37% (18/ 49) in intestinal metaplasia. The difference is significant with gastric cancer (X2=12.06, P<0.05). In colon carcinoma, the mycoplasma infection ratio was 55.1% (32/58), but it was 20.9% (10/49) in adenomarous polyp (X2=13.46, P<0.005).Gastric and colon cancers with high differentiation had a higher mycoplasma infection ratio than those with low differentiation (P< 0.05). Mycoplasma infection in esophageal cancer, lung cancer, breast cancer and glioma was 50.9% (27/53), 52.6% (31/ 59), 39.7%(25/63) and 41% (38/91), respectively. The mycoplasma DNA was successfully amplified with the DNA extracted from the cancer tissues that were positive for mycoplasma infection (detected with antibody PD4).CONCLUSION There was high correlation between mycoplasma infection and different cancers, which suggests the possibility of an association between the two. The mechanism involved in oncogenesis by mycoplasma remains unknown. | Su Huang Ji You Li Jan Wu Lin Meng Cheng Chao Shou Beijing Institute for Cancer Research, Peking University School of Oncology, Beijing 100034. ChinaSu Huang, received B. A from Jiangxi Medical College of China in 1994. Now she is a graduate student pursuing a Ph. D degree at the Peking University School of Oncology. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 2 | Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice显示文摘To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5 DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5 antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5 vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5 induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5 protein or the inactive elementary body in vitro. pcDNA3.1-pORF5-vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5 vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5-immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P<0.05). Together, these results demonstrated that immu- nization with pORF5 DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge. | LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 Xiangya School of Medicine, Central South University, Changsha 410078, China 2 Department of Microbiology and Immunology, University of South China, Hengyang 421001, China 3 University of Texas Health Science Center at San Antonio, TX 78229, USA | 2008 | Science China(Life Sciences)2008，51，11： | 16 |
| 3 | Automated detection of lunar craters based on object-oriented approach显示文摘The object-oriented approach is a powerful method in making classification. With the segmentation of images to objects, many features can be calculated based on the objects so that the targets can be distinguished. However, this method has not been applied to lunar study. In this paper we attempt to apply this method to detecting lunar craters with promising results. Craters are the most obvious features on the moon and they are important for lunar geologic study. One of the important questions in lunar research is to estimate lunar surface ages by examination of crater density per unit area. Hence, proper detection of lunar craters is necessary. Manual crater identification is inefficient, and a more efficient and effective method is needed. This paper describes an object-oriented method to detect lunar craters using lunar reflectance images. In the method, many objects were first segmented from the image based on size, shape, color, and the weights to every layer. Then the feature of 'contrast to neighbor objects' was selected to identify craters from the lunar image. In the next step, by merging the adjacent objects belonging to the same class, almost every crater can be taken as an independent object except several very big craters in the study area. To remove the crater rays diagnosed as craters, the feature of 'length/width' was further used with suitable parameters to finish recognizing craters. Finally, the result was exported to ArcGIS for manual modification to those big craters and the number of craters was acquired. | YUE ZongYu1,2,3,4, LIU JianZhong3 & WU GanGuo2 1 Institute of Remote Sensing Application, Chinese Academy of Sciences, Beijing 100101, China 2 China University of Geosciences (Beijing), Beijing 100083, China 3 National Astronomical Observatories of Chinese Academy of Sciences, Beijing 100012, China 4 Department of Geological Sciences, The University of Texas at San Antonio, San Antonio, TX 78249, U.S.A | 2008 | Chinese Science Bulletin2008，53，23： | 12 |
| 4 | miRNAs modulate the drug response of tumor cells显示文摘Chemotherapy is one of the major treatments of malignant carcinomas. However,its efficiency is affected by both intrinsic and acquired resistance to anticancer drugs. The cellular mechanisms of drug resistance include the overexpression of energy-dependent transporters that eject anticancer drugs from cells such as p-glycoprotein and multidrug resistance related protein (MRP),the mutation of drug targets,the activation of DNA repair pathways,the defects in cellular death pathways and so on. The genetic and epigenetic changes of these genes can lead to cancer drug resistance. Among these mechanisms,microRNAs (miRNAs) which are critical and essential for many important processes such as development,differentiation,and even carcinogenesis have been reported to regulate the chemosen-sitivity of tumor cells. In this paper we briefly review the relationship between miRNA and cancer drug resistance. | WU XueMei1,3 & XIAO HuaSheng1,2 1 Center of Functional Genomics,Key Laboratory of System Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2 National Engineering Center for Biochip at Shanghai,Shanghai 201203,China 3 Graduate University of Chinese Academy of Sciences,Shanghai 200031,China | 2009 | Science China(Life Sciences)2009，52，9： | 6 |
| 5 | Oligomeric Procyanidins Induce Generation of Reactive Oxygen Species and Collapse of Mitochchondrial Membrane Potential in Glioblastoma Cell Lines显示文摘Objective The aim of the present study was to clarify the mechanism underlying glioma cell death upon oligomeric procyanidins (F2) exposure. Methods The cytotoxicity of F2 on U87 (human malignant glioblastoma cell line) and C6 (rat glioma cell line) cancer cells was evaluated, and changes of mitochondrial membrane potential (MMP) and production of reactive oxygen species (ROS) in drug-treated cells were monitored. Moreover, morphological changes associated with F2-induced cells death were examined. Results F2 induced a concentration-dependent increase in ROS production and decrease in MMP. Furthermore, pre-incubation with N-acetylcysteine (NAC) and rotenone (Rt), resulted in partial inhibition of F2-induced ROS generation and marked attenuation of cell death and the cytoplasmic vacuolization induced by F2. In addition, pretreatment with Rt markedly attenuated the MMP loss in F2-treated cells. However, pretreatment with NAC only markedly attenuated the MMP loss in F2-treated C6 cells. Conclusion The increase in ROS level is at least one of mechanisms associated with F2-induced glioma cell death as well as the cytoplasmic vacuolization formation that contribute to the cytotoxicity of F2 in glioma cells. | ZHANG Feng-jiao1, YANG Jing-yu1, MOU Yan-hua1, SUN Bao-shan2, WANG Ji-ming3, WANG Fang1, WU Chun-fu1 1. Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China 2. Estaco Vitivinícola Nacional, Instituto National de Investigaco Agrária, Dois Portos 2565-191, Portugal 3. Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick Maryland 21702, USA | 2009 | Chinese Herbal Medicines2009，1，1： | 3 |
| 6 | Neuropathy and cognitive impairment following vaccination with the OspA protein of Borrelia burgdorferi显示文摘 | Latov N Wu AT Chin RL | 2004 | J Peripher Nerv Syst2004，9，3： | 1 |
| 7 | Desmosdumotin C, a novel cytotoxic principle from Desmos dumosus 显示文摘 | Wu JH Mcphail AT Bastow KF | 2002 | Tetrahedron Let2002，43，： | 1 |
| 8 | The effect of hypothermia on induction of heat protein (HSP)-72 in ischemic brain显示文摘 | Kumar K Wu X Evans AT | 1995 | Metab Brain Dis1995，10，4： | 1 |
| 9 | Inhibition of gastric cancer invasion and metastasis by PLA2G2A, a novel be- ta-catenin/TCF target gene显示文摘 | GANESAN K IVANOVA T WU Y et at | 2008 | Cancer Res2008，68，11： | 1 |
| 10 | Oxidative stress response elicited by mitochondrial dysfunction: hnplication in the pathophysiology of aging 显示文摘 | Wang CH Wu SB Wu YT et at | 2013 | Experimental Biology and Medicine2013，238，5： | 1 |
| 11 | Twinning-Detwinning Behavior during the Strain-Controlled Low-Cycle Fatigue Testing of a Wrought Magnesium Alloy, ZK60A显示文摘 | L Wu A Jain D W Brown et at | 2008 | Acta Materialia2008，56，： | 1 |
| 12 | Effect of biochar amendment on soil thermal conductivity, reflectance, and temperature显示文摘 | Zhang Q Wang Y Wu Y et at | 2012 | Soil Science Soci-ety of A merica Journal2012，77，： | 1 |
| 13 | Effects of pipe materials on chlorine- resistant biofilm formation under long-term high chlorine level 显示文摘 | Zhu Z Wu C Zhong D et at | 2014 | Appl Biochem Biotechnol2014，173，： | 1 |
| 14 | Progress in the detection of human genome structural variations显示文摘The emerging of high-throughput and high-resolution genomic technologies led to the detection of submicroscopic variants ranging from 1 kb to 3 Mb in the human genome.These variants include copy number variations(CNVs),inversions,insertions,deletions and other complex rearrangements of DNA sequences.This paper briefly reviews the commonly used technologies to discover both genomic structural variants and their potential influences.Particularly,we highlight the array-based,PCR-based and sequencing-based assays,including array-based comparative genomic hybridization(aCGH),representational oligonucleotide microarray analysis(ROMA),multiplex amplifiable probe hybridization(MAPH),multiplex ligation-dependent probe amplification(MLPA),paired-end mapping(PEM),and next-generation DNA sequencing technologies.Furthermore,we discuss the limitations and challenges of current assays and give advices on how to make the database of genomic variations more reliable. | WU XueMei1,3 & XIAO HuaSheng1,2 1 Center of Functional Genomics,Key Laboratory of System Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2 National Engineering Center for Biochip at Shanghai,Shanghai 201203,China 3 Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China | 2009 | Science China(Life Sciences)2009，52，6： | 1 |
| 15 | Sedimentary geochemical evidence for recent eutrophication of Lake Chenghai, Yunnan, China 显示文摘 | Wu J L Michael K Gagan et at | 2004 | Journal of Paleolimnology2004，32，： | 1 |
| 16 | The granularity of impurities in the fuel all resistance test显示文摘 | Zhang X Y Wu P Gong Y A et at | 2006 | Jiangxi Electric Power2006，2，23： | 1 |
| 17 | Synthesis of ZnO nanorod and the annealing effect on its photoluminescence property显示文摘 | Wu L L Wu Y S Pan X R at al | 2006 | Optic Mater2006，28，4： | 1 |
| 18 | Anomalous overexpression of p27Kip1 in sporadic pancreatic endocrine tumors显示文摘 | Guo SS Wu X Shimoide AT | 2001 | J Surg Res2001，96，2： | 1 |
| 19 | Enhancing Dialogue,Exchange and Cooperation(Excerpts)显示文摘EnhancingDialogue,ExchangeandCooperatio(Excerpts)ByWuXingtang(Secretary-generalofCAFIU,ProfessoratthePeople'sUniversityofChin?.. | Wu Xingtang(Secretary-general of CAFIU,Professor at the People’s University of China) | 1997 | International Understanding1997，，2： | 1 |
| 20 | Dual-Energy CT Angiography in the Evaluation of Intracranial Aneurysms: Image Quality, Radiation Dose, and Comparison With 3D Rotational Digital Subtraction Angiography显示文摘 | Zhang L J Wu SY Niu JB et aT | 2010 | American Roentgen Ray Society2010，194，1： | 1 |
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