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| 1 | Plecanatide and dolcanatide, novel guanylate cyclase-C agonists, ameliorate gastrointestinal inflammation in experimental models of murine colitis显示文摘AIM: To evaluate the effect of orally administeredplecanatide or dolcanatide, analogs of uroguanylin, on amelioration of colitis in murine models.METHODS: The cyclic guanosine monophosphate(cG MP) stimulatory potency of plecanatide and dolcanatide was measured using a human colon carcinoma T84 cellbased assay. For animal studies all test agents were formulated in phosphate buffered saline. Sulfasalazine or 5-amino salicylic acid(5-ASA) served as positive controls. Effect of oral treatment with test agents on amelioration of acute colitis induced either by dextran sulfate sodium(DSS) in drinking water or by rectal instillation of trinitrobenzene sulfonic(TNBS) acid, was examined in BALB/c and/or BDF1 mice. Additionally, the effect of orally administered plecanatide on the spontaneous colitis in T-cell receptor alpha knockout(TCRα-/-) mice was also examined. Amelioration of colitis was assessed by monitoring severity of colitis, disease activity index and by histopathology. Frozen colon tissues were used to measure myeloperoxidase activity.RESULTS: Plecanatide and dolcanatide are structurally related analogs of uroguanylin, which is an endogenous ligand of guanylate cyclase-C(GC-C). As expected from the agonists of GC-C, both plecanatide and dolcanatide exhibited potent cG MP-stimulatory activity in T84 cells. Once-daily treatment by oral gavage with either of these analogs(0.05-0.5 mg/kg) ameliorated colitis in both DSS and TNBS-induced models of acute colitis, as assessed by body weight, reduction in colitis severity(P < 0.05) and disease activity index(P < 0.05). Amelioration of colitis by either of the drug candidates was comparable to that achieved by orally administered sulfasalazine or 5-ASA. Plecanatide also effectively ameliorated colitis in TCRα-/- mice, a model of spontaneous colitis. As dolcanatide exhibited higher resistance to proteolysis in simulated gastric and intestinal juices, it was selected for further studies. CONCLUSION: This is the first-ever study reporting the therapeutic utility of GC-C agonists as a new class of orally delivered and mucosally active drug candidates for the treatment of inflammatory bowel diseases. | Kunwar Shailubhai Vaseem Palejwala Krishna Priya Arjunan Sayali Saykhedkar Bradley Nefsky John A Foss Stephen Comiskey Gary S Jacob Scott E Plevy | 2015 | World Journal of Gastrointestinal Pharmacology and Therapeutics2015，6，4： | 5 |
| 2 | Plecanatide-mediated activation of guanylate cyclase-C suppresses inflammation-induced colorectal carcinogenesis in Apc+/Min-FCCC mice显示文摘AIM To evaluate the effect of orally administered plecanatide on colorectal dysplasia in Apc^(+/Min-FCCC) mice with dextran sodium sulfate(DSS)-induced inflammation. METHODS Inflammation driven colorectal carcinogenesis was induced in Apc^(+/Min-FCCC) mice by administering DSS in their drinking water. Mice were fed a diet supplemented with plecanatide(0-20 ppm) and its effect on the multiplicity of histopathologically confirmed polypoid,flat and indeterminate dysplasia was evaluated. Plecanatide-mediated activation of guanylate cyclase-C(GC-C) signaling was assessed in colon tissues by measuring cyclic guanosine monophosphate(cG MP) by ELISA, protein kinase G-II and vasodilator stimulated phosphoprotein by immunoblotting. Ki-67, c-myc and cyclin D1 were used as markers of proliferation. Cellular levels and localization of b-catenin in colon tissues were assessed by immunoblotting and immunohistochemistry, respectively. Uroguanylin(UG) and GC-C transcript levels were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR). A mouse cytokine array panel was used to detect cytokines in the supernatant of colon explant cultures. RESULTS Oral treatment of Apc^(+/Min-FCCC) mice with plecanatide produced a statistically significant reduction in the formation of inflammation-driven polypoid, flat and indeterminate dysplasias. This anti-carcinogenic activity of plecanatide was accompanied by activation of cG MP/GC-C signaling mediated inhibition of Wnt/b-catenin signaling and reduced proliferation. Plecanatide also decreased secretion of pro-inflammatory cytokines(IL-6, IL-1 TNF), chemokines(MIP-1, IP-10) and growth factors(GCSF and GMCSF) from colon explants derived from mice with acute DSS-induced inflammation. The effect of plecanatidemediated inhibition of inflammation/dysplasia on endogenous expression of UG and GC-C transcripts was measured in intestinal tissues. Although GC-C expression was not altered appreciably, a statistically significant increase in the level of UG transcripts was detected in the proximal small intestine and colon, potentially due to a reduction in intestinal inflammation and/or neoplasia. Taken together, these results suggest that reductions in endogenous UG, accompanied by dysregulation in GC-C signaling, may be an early event in inflammation-promoted colorectal neoplasia; an event that can potentially be ameliorated by prophylactic intervention with plecanatide.CONCLUSION This study provides the first evidence that orally administered plecanatide reduces the multiplicity of inflammation-driven colonic dysplasia in mice, demonstrating the utility for developing GC-C agonists as chemopreventive agents. | Wen-Chi L Chang Shet Masih Anusha Thadi Viren Patwa Apoorva Joshi Harry S Cooper Vaseem A Palejwala Margie L Clapper Kunwar Shailubhai | 2017 | World Journal of Gastrointestinal Pharmacology and Therapeutics2017，8，1： | 5 |
| 3 | Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models显示文摘AIM To investigate the effects of plecanatide and dolcanatide on maintenance of paracellular permeability, integrity of tight junctions and on suppression of visceral hypersensitivity. METHODS Transport of fluorescein isothiocyanate(FITC)-dextran was measured to assess permeability across cell monolayers and rat colon tissues. Effects of plecanatide and dolcanatide on the integrity of tight junctions in Caco-2 and T84 monolayers and on the expression and localization of occludin and zonula occludens-1(ZO-1) were examined by immunofluorescence microscopy. Anti-nociceptive activity of these agonists was evaluated in trinitrobenzene sulfonic acid(TNBS)-induced inflammatory as well as in non-inflammatory partial restraint stress(PRS) rat models. Statistical significance between the treatment groups in the permeability studies were evaluated using unpaired t-tests.RESULTS Treatment of T84 and Caco-2 monolayers with lipopolysaccharide(LPS) rapidly increased permeability, which was effectively suppressed when monolayers were also treated with plecanatide or dolcanatide. Similarly, when T84 and Caco-2 monolayers were treated with LPS, cell surface localization of tight junction proteins occludin and ZO-1 was severely disrupted. When cell monolayers were treated with LPS in the presence of plecanatide or dolcanatide, occludin and ZO-1 were localized at the cell surface of adjoining cells, similar to that observed for vehicle treated cells. Treatment of cell monolayers with plecanatide or dolcanatide without LPS did not alter permeability, integrity of tight junctions and cell surface localization of either of the tight junction proteins. In rat visceral hypersensitivity models, both agonists suppressed the TNBS-induced increase in abdominal contractions in response to colorectal distension without affecting the colonic wall elasticity, and both agonists also reduced colonic hypersensitivity in the PRS model. CONCLUSION Our results suggest that activation of GC-C signaling might be involved in maintenance of barrier function, possibly through regulating normal localization of tight junction proteins. Consistent with these findings, plecanatide and dolcanatide showed potent antinociceptive activity in rat visceral hypersensitivity models. These results imply that activation of GC-C signaling may be an attractive therapeutic approach to treat functional constipation disorders and inflammatory gastrointestinal conditions. | Illona-Marie Boulete Anusha Thadi Catherine Beaufrand Viren Patwa Apoorva Joshi John A Foss E Priya Eddy Helene Eutamene Vaseem A Palejwala Vassilia Theodorou Kunwar Shailubhai | 2018 | World Journal of Gastroenterology2018，24，17： | 5 |
| 4 | THE NUTRITIONAL VALUE OF PLEUROTUS OSTREATUS (JACQ.:FR.) KUMM CULTIVATED ON DIFFERENT LIGNOCELLULOSIC AGRO-WASTES显示文摘 | Patil Shyam Sopanrao Ahmed Syed Abrar Telang Suresh Manoharrao Baig Mirza Mushtaq Vaseem | 2010 | Innovative Romanian Food Biotechnology2010，，： | 1 |
| 5 | Apoptosis induced by copper oxide quantum dots in cultured C2C12 cells via caspase 3 and caspase 7:a study on cytotoxicity assessment显示文摘 | Amna T Van Ba H Vaseem M | 2013 | Appl Microbiol Biotechnol2013，97，12： | 1 |
| 6 | Fully inkjet-printed microwave passive electronics显示文摘Fully inkjet-printed three-dimensional(3D)objects with integrated metal provide exciting possibilities for on-demand fabrication of radio frequency electronics such as inductors,capacitors,and filters.To date,there have been several reports of printed radio frequency components metallized via the use of plating solutions,sputtering,and low-conductivity pastes.These metallization techniques require rather complex fabrication,and do not provide an easily integrated or versatile process.This work utilizes a novel silver ink cured with a low-cost infrared lamp at only 80℃,and achieves a high conductivity of 1×10^(7) S m^(−1).By inkjet printing the infrared-cured silver together with a commercial 3D inkjet ultraviolet-cured acrylic dielectric,a multilayer process is demonstrated.By using a smoothing technique,both the conductive ink and dielectric provide surface roughness values of <500 nm.A radio frequency inductor and capacitor exhibit state-of-the-art quality factors of 8 and 20,respectively,and match well with electromagnetic simulations.These components are implemented in a lumped element radio frequency filter with an impressive insertion loss of 0.8 dB at 1 GHz,proving the utility of the process for sensitive radio frequency applications. | Garret McKerricher Mohammad Vaseem Atif Shamim | 2017 | Microsystems & Nanoengineering2017，3，1： | 1 |
| 7 | 显示文摘 | Vaseem M Lee K M Hong A R | 2012 | Applied Materials Interfaces2012，4，6： | 1 |
| 8 | Parametric study of cost-effective synthesis of crystalline copper nanoparticles and their crystallographic characterization显示文摘 | Vaseem M Lee K M Kim D Y | 2011 | Materials Chemistry & Physics2011，125，： | 1 |
| 9 | 查看详情显示文摘 | Vaseem M Umar A Kim S H | | 0，，： | 1 |
| 10 | Thermoelectric properties of SrTiO3 nano-particles dispersed indium sele- hide bulk composites 显示文摘 | Min H L Jong S R Vaseem M | 2013 | Applied Physics Letters2013，102，22： | 1 |
| 11 | Apoptosis induced by copper oxide quantum dots in cultured C2C12 cells via caspase 3 and caspase 7 : a study on cytotoxicity assessment 显示文摘 | Touseef A Hoa V B Vaseem M | 2013 | Applied Microbi- ology & Biotechnology2013，97，12： | 1 |
| 12 | Parametric study of cost-effective synthesis of crystalline copper nanoparticles and their crystallographic characterization显示文摘 | Vaseem M Lee K M Kim D Y | 2011 | Mater Chem Phys2011，125，3： | 1 |
| 13 | Parametric study of cost- effective synthesis of crystalline copper nanoparticles and their crystallographic characterization显示文摘 | Vaseem M Lee K M Kim D Y | 2011 | Materials Chemistry and Physics2011，125，3： | 1 |
| 14 | Inkjetprinted fractal-connected electrodes with silvernanoparticle ink 显示文摘 | VASEEM M LEE K M HONG A R | 2012 | ACS Applied Materials &Interfaces2012，4，6： | 1 |
| 15 | Flexible and reconfigurable radio frequency electronics realized by high-throughput screen printing of vanadium dioxide switches显示文摘Smart materials that can change their properties based on an applied stimulus are in high demand due to their suitability for reconfigurable electronics,such as tunable filters or antennas.In particular,materials that undergo a metal–insulator transition(MIT),for example,vanadium dioxide(VO 2)(M),are highly attractive due to their tunable electrical and optical properties at a low transition temperature of 68°C.Although deposition of this material on a limited scale has been demonstrated through vacuum-based fabrication methods,its scalable application for large-area and high-volume processes is still challenging.Screen printing can be a viable option because of its high-throughput fabrication process on flexible substrates.In this work,we synthesize high-purity VO 2(M)microparticles and develop a screen-printable VO 2 ink,enabling the large-area and high-resolution printing of VO 2 switches on various substrates.The electrical properties of screen-printed VO 2 switches at the microscale are thoroughly investigated under both thermal and electrical stimuli,and the switches exhibit a low ON resistance of 1.8 ohms and an ON/OFF ratio of more than 300.The electrical performance of the printed switches does not degrade even after multiple bending cycles and for bending radii as small as 1mm.As a proof of concept,a fully printed and mechanically flexible band-pass filter is demonstrated that utilizes these printed switches as reconfigurable elements.Based on the ON and OFF conditions of the VO 2 switches,the filter can reconfigure its operating frequency from 3.95 to 3.77 GHz without any degradation in performance during bending. | Weiwei Li Mohammad Vaseem Shuai Yang Atif Shamim | 2020 | Microsystems & Nanoengineering2020，6，1： | 1 |
| 16 | Low-temperature growth and properties of flower-shapedα-Ni(OH)2and Ni O structures composed of thin nanosheets networks显示文摘 | Al-Hajry A Umar A Vaseem M | 2008 | Superlattices and Microstructures2008，44，2： | 1 |
| 17 | Flower-shaped CuO nanostructures : structural, photocatalytic and XANES studies 显示文摘 | VASEEM M UMAR A HAHN Y B | 2008 | Catalysis Communications2008，10，1： | 1 |
| 18 | 查看详情显示文摘 | Hajry A.A Umar A Vaseem M Assiri M.S Tantawy F.E Bououdina M Heniti S.A Hahn Y.B | | 0，，： | 1 |
| 19 | Stress Detector Supported Galvanic Skin Response System with IoT and LabVIEW GUI显示文摘Stress is now a serious disease that exists due to changes in working life and food ecosystems around the world.In general,it is difficult for a person to know if they are under stress.According to previous research,temperature,heart rate variability(HRV),humidity,and blood pressure are used to assess stress levels with the use of instruments.With the development of sensor technology and wireless connectivity,people around the world are adopting and using smart devices.In this study,a bio signal detection device with Internet of Things(IoT)capability with a galvanic skin reaction(GSR)sensor is proposed and built for real-time stress monitoring.The proposed device is based on an Arduino controller and Bluetooth communication.To evaluate the performance of the system,physical stress is created on 10 different participants with three distinct tasks namely reading,visualizing the timer clock,and watching videos.MATLAB analysis is performed for identifying the three different levels of stress and obtaining the threshold values as if the person GSR voltage i.e.,relaxed for<1.75 volts;Normal:between 1.75 and 1.44 volts and stressed:>1.44 volts.In addition,LabVIEW is used as a data acquisition system,and a Blueterm mobile application is also used to view the sensor reading received from the device through Bluetooth communication. | Rajesh Singh Anita Gehlot Ritika Saxena Khalid Alsubhi Divya Anand Irene Delgado Noya Shaik Vaseem Akram Sushabhan Choudhury | 2023 | Computers, Materials & Continua2023，，1： | 0 |
| 20 | Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju(Fr.) Singer显示文摘Pleurotus sajor-caju (Fr.) Singer was cultivated on selected agro wastes viz. cotton stalks, groundnut haulms, soybean straw, pigeon pea stalks and leaves and wheat straw, alone or in combinations. Cotton stalks, pigeon pea stalks and wheat straw alone or in combination were found to be more suitable than groundnut haulms and soybean straw for the cultivation. Organic supplements such as groundnut oilseed cake, gram powder and rice bran not only affected growth parameters but also increased yields. Thus bioconversion of lignocellulosic biomass by P. sajor-caju offers a promising way to convert low quality biomass into an improved human food. | MANE Vijay Panjabrao PATIL Shyam Sopanrao SYED Abrar Ahmed BAIG Mirza Mushtaq Vaseem | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 0 |
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