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| 1 | Endothelial progenitor cells, potential biomarkers for diagnosis and prognosis of ischemic stroke: protocol for an observational case-control study显示文摘Ischemic stroke is a devastating,life altering event which can severely reduce patient quality of life.Despite years of research there have been minimal therapeutic advances.Endothelial progenitor cells(EPCs),stem cells involved in both vasculogenesis and angiogenesis,may be a potential therapeutic target.After a stroke,EPCs migrate to the site of ischemic injury to repair cerebrovascular damage,and their numbers and functional capacity may determine patients'outcome.This study aims to determine whether the number of circulating EPCs and their functional aspects may be used as biomarkers to identify the type(cortical or lacunar)and/or severity of ischemic stroke.The study will also investigate if there are any differences in these characteristics between healthy volunteers over and under 65 years of age.100 stroke patients(50 lacunar and 50 cortical strokes)will be recruited in this prospective,observational case-controlled study.Blood samples will be taken from stroke patients at baseline(within 48 hours of stroke)and days 7,30 and90.EPCs will be counted with flow cytometry.The plasma levels of pro-and anti-angiogenic factors and inflammatory cytokines will also be determined.Outgrowth endothelial cells will be cultured to be used in tube formation,migration and proliferation functional assays.Primary outcome is disability or dependence on day 90 after stroke,assessed by the modified Rankin Scale.Secondary outcomes are changes in circulating EPC numbers and/or functional capacity between patient and healthy volunteers,between patient subgroups and between elderly and young healthy volunteers.Recruitment started in February 2017,167 participants have been recruited.Recruitment will end in November 2019.West Midlands-Coventry&Warwickshire Research Ethics Committee approved this study(REC number:16/WM/0304)on September8,2016.Protocol version:2.0.The Bayraktutan Dunhill Medical Trust EPC Study was registered in ClinicalTrials.gov(NCT02980354)on November 15,2016.This study will determine whether the number of EPCs can be used as a prognostic or diagnostic marker for ischemic strokes and is a step towards discovering if transplantation of EPCs may aid patient recovery. | Kamini Rakkar Othman Othman Nikola Sprigg Philip Bath Ulvi Bayraktutan | 2020 | Neural Regeneration Research2020，15，7： | 7 |
| 2 | Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage显示文摘Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can effectively replace dead or dying endothelial cells to restore blood-brain barrier integrity is of paramount importance in stroke medicine.Although endothelial progenitor cells(EPCs)represent one such agents,their rarity in peripheral blood severely limits their adequate isolation and therapeutic use for acute ischemic stroke which necessitate their ex vivo expansion and generate early EPCs and outgrowth endothelial cells(OECs)as a result.Functional analyses of these cells,in the present study,demonstrated that only OECs endocytosed DiI-labelled acetylated low-density lipoprotein and formed tubules on matrigel,prominent endothelial cell and angiogenesis markers,respectively.Further analyses by flow cytometry demonstrated that OECs expressed specific markers for sternness(CD34),immaturity(CD133)and endothelial cells(CD31)but not for hematopoietic cells(CD45).Like BMECs,OECs established an equally tight in vitro model of human BBB with astrocytes and pericytes,suggesting their capacity to form tight junctions.Ischemic injury mimicked by concurrent deprivation of oxygen and glucose(4 hours)or deprivation of oxygen and glucose followed by reperfusion(20 hours)affected both barrier integrity and function in a similar fashion as evidenced by decreases in transendothelial electrical resistance and increases in paracellular flux,respectively.Wound scratch assays comparing the vasculoreparative capacity of cells revealed that,compared to BMECs,OECs possessed a greater proliferative and directional migratory capacity.In a triple culture model of BBB established with astrocytes,pericytes and BMEC,exogenous addition of OECs effectively repaired the damage induced on endothelial layer in serum-free conditions.Taken together,these data demonstrate that OECs may effectively home to the site of vascular injury and repair the damage to maintain(neuro)vascular homeostasis during or after a cerebral ischemic injury. | Rais Reskiawan Abdulkadir Mansour Alwjwaj Othman Ahmad Othman Kamini Rakkar Ulvi Bayraktutan | 2020 | Neural Regeneration Research2020，15，6： | 5 |
| 3 | The secretome of endothelial progenitor cells: a potential therapeutic strategy for ischemic stroke显示文摘Ischemic stroke continues to be a leading cause of mortality and morbidity in the world. Despite recent advances in the field of stroke medicine, thrombolysis with recombinant tissue plasminogen activator remains as the only pharmacological therapy for stroke patients. However, due to short therapeutic window(4.5 hours of stroke onset) and increased risk of hemorrhage beyond this point, each year globally less than 1% of stroke patients receive this therapy which necessitate the discovery of safe and efficacious therapeutics that can be used beyond the acute phase of stroke. Accumulating evidence indicates that endothelial progenitor cells(EPCs), equipped with an inherent capacity to migrate, proliferate and differentiate, may be one such therapeutics. However, the limited availability of EPCs in peripheral blood and early senescence of few isolated cells in culture conditions adversely affect their application as effective therapeutics. Given that much of the EPC-mediated reparative effects on neurovasculature is realized by a wide range of biologically active substances released by these cells, it is possible that EPC-secretome may serve as an important therapeutic after an ischemic stroke. In light of this assumption, this review paper firstly discusses the main constituents of EPC-secretome that may exert the beneficial effects of EPCs on neurovasculature, and then reviews the currently scant literature that focuses on its therapeutic capacity. | Mansour Alwjwaj Rais Reskiawan AKadir Ulvi Bayraktutan | 2021 | Neural Regeneration Research2021，16，8： | 3 |
| 4 | The effect of pH, buffer type and drug concentration on the photodegradation of ciprofloxacin显示文摘 | Tomiainen K Tammilehto S Ulvi V | 1996 | International Journal of Pharmaceutics1996，132，： | 1 |
| 5 | Multilocular cystic nephroma: an unusual localization显示文摘 | Ali Riza Kural Can ?bek Gülsen ?zbay Ali Ulvi ?nder | 1998 | Urology1998，，5： | 1 |
| 6 | Lead removal by polyacrylamide- bentonite and zeolite composites: Effect of phytic acid immobi- lization显示文摘 | Ulvi Ulusoy Selcuk Simsek | 2005 | Journal of Hazardous Materials2005，127，： | 1 |
| 7 | Evaluatioachinability of austempered ductile irons in terms of cuttinn of mg forces and surface quality 显示文摘 | Ulvi Seker Hasan Hasirci | 2006 | Journal of Materials Processing Technology2006，，173： | 1 |
| 8 | Evaluation of tool wear when machining SiC p-reinforced Al-2014 alloy matrix composites显示文摘 | C Ibrahim T Mehmet S Ulvi | 2004 | Materials and Design2004，25，： | 1 |
| 9 | The role of nickel accumulation and epithelial cell proliferation in orthodontic treatment-induced gingival overgrowth显示文摘 | Ulvi Kahraman Gursoy | 2007 | European Journal of Orthodontics2007，，29： | 1 |
| 10 | Twin pregnancy with a complete hydatidiform mole and co-existent live fetus: two case reports and review of the literature显示文摘 | Kenan Dolapcioglu Arif Gungoren Sibel Hakverdi Ali Ulvi Hakverdi Ertugrul Egilmez | 2009 | Archives of Gynecology and Obstetrics2009，，3： | 1 |
| 11 | The Relationship of Heart Rate Variability with Severity and Prognosis of Cirrhosis显示文摘 | Fehmi Ates Ergun Topal Feridun Kosar Melih Karincaoglu Bulent Yildirim Yuksel Aksoy Murat Aladag Murat M. M. Harputluoglu Ulvi Demirel Hakan Alan Fatih Hilmioglu | 2006 | Digestive Diseases and Sciences2006，，9： | 1 |
| 12 | Determination of sensitive electro- physiology parameters at follow-up of different steroid treatments of carpal tunnel syndrome 显示文摘 | Aygtll R Ulvi H Karatay S | 2005 | J Cliu Neurophyslol2005，22，3： | 1 |
| 13 | Comimmotts infusion of mizda-zolam in the treatment of refraetory generalized convulsive status epilepticus显示文摘 | Ulvi H YoldasT MungeaB et a1 | 2002 | Neurol Sci2002，23，4： | 1 |
| 14 | Repair of a wide lower extremity defect with cross--leg free transfer of latis- simus dorsi and serratus anterior combined flap: a case report显示文摘 | Gursel Turgut Mahmut Ulvi Kayah Ozkan Kose | 2010 | Strategies Trauma Limb Reconstr2010，5，3： | 1 |
| 15 | The effect of pH,buffer type and drug concentration on the photodegradation of ciprofloxacin显示文摘 | Kirsti Torniainen Seije Tammilehto Veikko Ulvi | 1996 | International Journal of Pharmaceutics1996，132，1： | 1 |
| 16 | Determination of sensitive electrophysiologic parameters at follow up of different steroid treaments of carpal tunnel syndrome显示文摘 | Aygul R Ulvi H Karatay S | 2005 | J Clin Neurophysiol2005，22，3： | 1 |
| 17 | Magnesium,zinc and copper contents in hair and their serum concentrations in patients with epilepsy显示文摘 | Ulvi H Yiˇgiter R Yolda?T | 2013 | East J Med2013，7，2： | 1 |
| 18 | Thallium adsorption onto polyacryamide- aluminosilicate composites: a TI isotope tracer study显示文摘 | Zeynep M S Ulvi U | 2010 | Chemical Engineering Journal2010，162，1： | 1 |
| 19 | Continuous infusion of mizdazolam in the treatment of refractory generalized convulsive status epilepticus显示文摘 | Ulvi H Yoldas T Mungen B | 2002 | Neurol Sci2002，23，： | 1 |
| 20 | Profiles of faecal output of rare earth elements and stable isotope tracers of iron and zinc after oral administration显示文摘 | Ulvi U John EW | 2000 | British Journal of Nutrition2000，84，： | 1 |
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