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您的检索式：作者名="Tim Berendsen"
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| 1 | Strategies to rescue steatotic livers before transplantation in clinical and experimental studies显示文摘The shortage of donor livers has led to an increased use of organs from expanded criteria donors. Included are livers with steatosis, a metabolic abnormality that increases the likelihood of graft complications posttransplantation. After a brief introduction on the etiology, pathophysiology, categories and experimental models of hepatic steatosis, we herein review the methods to rescue steatotic donor livers before transplantation applied in clinical and experimental studies. The methods span the spectrum of encouraging donor weight loss, employing drug therapy, heat shock preconditioning, ischemia preconditioning and selective anesthesia on donors, and the treatment on isolated grafts during preservation. These methods work at different stages of transplantation process, although share similar molecular mechanisms including lipid metabolism stimulation through enzymes or nuclear receptor e.g. , peroxisomal proliferator-activated receptor, or anti-inflammation through suppressing cytokines e.g. , tumor necrosis factor-α, or antioxidant therapies to alleviate oxidative stress. This similarity of molecular mechanisms implies possible future attempts to reinforce each approach by repeating the same treatment approach at several stages of procurement and preservation, as well as utilizing these alternative approaches in tandem. | Qiang Liu Maria-Louisa Izamis Hongzhi Xu Tim Berendsen Martin Yarmush Korkut Uygun | 2013 | World Journal of Gastroenterology2013，19，29： | 1 |
| 2 | Multipolar radiofrequency ablation for colorectal liver metastases close to major hepatic vessels显示文摘 | Nikol Snoeren Maarten W. Nijkamp Tim Berendsen Klaas M. Govaert Charlotte S. van Kessel Inne H.M. Borel Rinkes Richard van Hillegersberg | 2013 | The Surgeon2013，，： | 1 |
| 3 | Layer-by-layer heparinization of decellularized liver matrices to reduce thrombogenicity of tissue engineered grafts显示文摘Background: Tissue-engineered liver grafts may offer a viable alternative to orthotopic liver transplantation and help overcome the donor organ shortage. Decellularized liver matrices (DLM) have a preserved vasculature and sustain hepatocellular function in culture, but graft survival after transplantation remains limited due to thrombogenicity of the matrix. Aim: To evaluate the effect of heparin immobilization on DLM thrombogenicity. Methods: Heparin was immobilized on DLMs by means of layer-by-layer deposition. Grafts with 4 or 8 bilayers and 2 or 4 g/L of heparin were recellularized with primary rat hepatocytes and maintained in culture for 5 days. Hemocompatibility of the graft was assessed by ex vivo diluted whole-blood perfusion and heterotopic transplantation. Results: Heparin was deposited throughout the matrix and the heparin content in the graft was higher with increasing number of bilayers and concentration of heparin. Recellularization and in vitro albumin and urea production were unaffected by heparinization. Resistance to blood flow during ex vivo perfusion was lower with increased heparinization and, macroscopically, no clots were visible in grafts with 8 bilayers. Following transplantation, flow through the graft was limited in all groups. Histological evidence of thrombosis was lower in heparinized DLMs, but transplantation of DLM grafts was not improved. Conclusions: Layer-by-layer deposition of heparin on a DLM is an effective method of immobilizing heparin throughout the graft and does not impede recellularization or hepatocellular function in vitro. Thrombogenicity during ex vivo blood perfusion was reduced in heparinized grafts and optimal with 8 bilayers, but transplantation remained unsuccessful with this method. Relevance for patients: Tissue engineered liver grafts may offer a viable solution to dramatic shortages in donor organs. | Bote G Bruinsma Yeonhee Kim Tim A Berendsen Sinan Ozer Martin L Yarmush Basak E Uygun | 2015 | Journal of Clinical & Translational Research2015，1，1： | 0 |
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