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| 1 | Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes显示文摘Objective: The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3 T3-L1 preadipocytes/adipocytes as a model.Methods: Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3 T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red 0 staining and visualized under a light microscope.Results: n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 μg/ml. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentrationdependent inhibitory effects on lipid accumulation in 3 T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 μmol/L, respectively. Both kaempferide and 4,2'-dihy droxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.Conclusion: C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3 T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids. | Supakanya Kumkarnjana Rutt Suttisri Ubonthip Nimmannit Thongchai Koobkokkruad Chutichot Pattamadilok Nontima Vardhanabhuti | 2018 | Journal of Integrative Medicine2018，16，6： | 2 |
| 2 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | Vimolmas Lipipun Masahiko Kurokawa Rutt Suttisri Pagorn Taweechotipatr Pornpen Pramyothin Masao Hattori Kimiyasu Shiraki | 2003 | Antiviral Research2003，，3： | 1 |
| 3 | Plant - derived' triterpenoid sweettness inhibitors显示文摘 | Rutt Suttisri IK- Soo Lee A Douglas Kinghorn | | 0，，： | 1 |
| 4 | A new antiviral and antimicrobial sesquiterpene from Glyptopetalum sclerocarpum显示文摘 | Sotanaphun U Lipipun V Suttisri R Bavovada R | 1999 | Planta Med1999，65，3： | 1 |
| 5 | Plant-Derived Triterpenoid Sweetness Inhibitors 显示文摘 | Suttisri R Lee I S Kinghorn A D | 1995 | Journal of Ethnopharmacology1995，47，4： | 1 |
| 6 | Plant-derived triterpenoidsweetness inhibitors显示文摘 | Suttisri R Lee I S Kinghom A D | 1995 | Joumal of ethnopharmacology1995，47，1： | 1 |
| 7 | Plant-derived triterpenoid sweetness inhibitors显示文摘 | Suttisri R Lee IS Kinghorn AD | 1995 | J Ethnopharmacol1995，，47： | 1 |
| 8 | Plant - derived triterpenoid sweetness inhibitors 显示文摘 | Butt Suttisri Ik - Soo Lee | 1995 | Journal of Ethnopharmacology1995，47，4： | 1 |
| 9 | Plant-derived triterpenoid sweetness inhibitors 显示文摘 | Rutt Suttisri Ik-Soo Lee A Douglas Kinghorn^* | 1995 | Journal of Ethnopharmacology1995，47，： | 1 |
| 10 | Douglas Kinghorn显示文摘 | Rutt Suttisri Ik-Soo Lee A | 1995 | Journal of Ethnopharmacology1995，47，： | 1 |
| 11 | Quinone - methide triterpenoids from Glyptopetalum scleracarpum显示文摘 | Sotanaphun U Suttisri R Lipipun V | 1998 | Phytochemistry1998，49，6： | 1 |
| 12 | Plaut-dedved triterpenoid sweetness inhibitors显示文摘 | SUTTISRI R LEE I S KINGHORN A D | 1995 | Journal of Ethnopharmacology1995，47，4： | 1 |
| 13 | Plant-derived triterpenoid sweetness inhibitors显示文摘 | RUTT SUTTISRI I-S L | 1995 | Journal of Ethnopharmacology1995，，47： | 1 |
| 14 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | LIPIPUN V KUROKAWA M SUTTISRI R | 2003 | Antiviral Res2003，60，3： | 1 |
| 15 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo显示文摘 | LIPIPUN V KUROKAWA M SUTTISRI R | 2003 | Antiviral Res2003，60，3： | 1 |
| 16 | Efficacy of Thai medicinal plant extracts against herpes simplex virus type 1 infection in vitro and in vivo 显示文摘 | Vimolmas Lipipun Masahiko Kurokawa Rutt Suttisri | 2003 | Antiviral Research2003，60，3： | 1 |
| 17 | Plant derived triterpenoidsweetness inhibitors显示文摘 | R Suttisri L Ik-Soo KA Douglas | 1995 | Journal of Ethnopharmacology1995，47，： | 1 |
| 18 | Plant-derived triterpenoidsweetness inhibitors 显示文摘 | Suttisri R Lee I S Kinghorn A D | 1995 | Journal of Ethnopharmacology1995，47，1： | 1 |
| 19 | Plant-derived triterpenoid sweetness inhibitors显示文摘 | SUTTISRI R LEE I S KINGHRN A D | 1995 | Journal of Ethnopharmacology1995，47，1： | 1 |
| 20 | Cytotoxic activity of the chemical constituents of Clerodendrum indicum and Clerodendrum villosum roots显示文摘Objective: The roots of two Thai medicinal plants, Clerodendrum indicum and Clerodendrum villosum are found in traditional medicine practices. The aim of this research was to preliminarily study the cytotoxicity of extracts of their roots, and the parts that possessed cytotoxic activity were separated on a chromatograph to identify their active compounds.Methods: The extracts of both plants were screened for cytotoxicity on the SW620 cell line and the compounds isolated from the active extracts were further evaluated for their cytotoxic activity against five human cancer cell lines, including SW620, Cha Go-K-1, Hep G2, KATO-III and BT-474 using the 3-(4,5-dime thyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay.Results: Dichloromethane extracts of C. indicum and C. villosum were active against the SW620 cell line.Triterpenoids were mostly obtained from the extracts of these plants(0.28% and 1.02%, respectively) and exhibited varying degrees of cytotoxicity and specificity against the tested cell lines. Two triterpenoids,oleanolic acid 3-acetate and betulinic acid, displayed moderate to strong cytotoxicity toward all cancer cell lines, with 50% inhibitory concentration(IC50) values of 1.66–20.49 mmol/L, whereas 3 b-hydroxyD:B-friedo-olean-5-ene and taraxerol were cytotoxic to only the SW620 cell line(IC50= 23.39 and2.09 mmol/L, respectively). Triterpenoid, lupeol, showed potent cytotoxicity on both SW620(IC50=1.99 mmol/L) and KATO-III cell lines(IC50= 1.95 mmol/L), while a flavonoid, pectolinarigenin, displayed moderate cytotoxicity against these cells(IC50= 13.05 and 24.31 mmol/L, respectively). Although the widely distributed steroid, stigmasterol, was effective against the SW620 cell line(IC50= 2.79 mmol/L)and b-sitosterol was also active against SW620(IC50= 11.26 mmol/L), BT-474(IC50= 14.11 mmol/L) and Hep G2 cancer cells(IC50= 20.47 mmol/L), none of the characteristic 24 b-ethylsteroids of either Clerodendrum species were shown to be cytotoxic.Conclusion: This study is the first report on the presence of cytotoxic triterpenoids from the roots of these medicinal plants, which have been used in herbal formulas as an antipyretic. Our findings support further in-depth study of this pharmacological activity as an anticancer agent. | Pathom Somwong Rutt Suttisri | 2018 | Journal of Integrative Medicine2018，16，1： | 0 |
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