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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | The prediction of helicopter rotor discrete frequency noise 显示文摘 | FARASSAT F SUCCI G P | 1983 | Vertica1983，7，4： | 1 |
| 3 | The lattic Boltzmann equation on irregular lattices显示文摘 | NANNELLI F SUCCI S | 1996 | J Statist Phys1996，68，34： | 1 |
| 4 | Intermittency and structure functions in channel flow turbulence显示文摘 | Toschi F Amati G Succi S | 1999 | Physics Review Letter1999，82，25： | 1 |
| 5 | Multiscale lattice Bohznmnn schemes with turbulence modeling 显示文摘 | FILIPPOVA o SUCCI S MAZZOCCO F | 2001 | Journal of Computational Physics2001，170，2： | 1 |
| 6 | Effect of CPPU and pollination on fruiting performance, fruit quality and storage life of kiwifruit显示文摘 | Costa G Succi F Quadretti R | 1997 | ActaHort1997，444，： | 1 |
| 7 | A review of propeller discrete frequency noise prediction technology with em- phasis on two current methods for time domain calcula- tions 显示文摘 | FARASSAT F SUCCI G P | 1980 | Journal of Sound and Vibration1980，71，3： | 1 |
| 8 | Lattice Gas Dynamics with Enhanced Collision显示文摘 | Higuera F Jimenez J Succi S | | 0，，： | 1 |
| 9 | Giant cell tumor of tendonsheath: study of 64 cases and review of literature 显示文摘 | Di Grazia S Succi G Fragetta F | 2013 | G Chir2013，34，56： | 1 |
| 10 | The prediction of helicopter rotor discrete frequency noise显示文摘 | FARASSAT F SUCCI G P | 1983 | Vertica1983，7，4： | 1 |
| 11 | Multiscale lattice Boltzmann schemes with turbulence modeling显示文摘 | Fillipova O Succi S Mazzocco F | 2001 | Journal of Computational Physics2001，170，2： | 1 |
| 12 | Effects of bud load and fruit thinning in the quantity and quality of fruiting in Hayward (Actinidia deliciosa )显示文摘 | Costa G Succi F Morigi M | 1995 | Rivista di Frutticoltura e di Ortofloricohura1995，57，4： | 1 |
| 13 | Multiscale Lattice Boltzmann Schemes with Turbulence Modeling显示文摘 | Filippova O Succi S Mazzocco F | 2001 | Journal of Computational Physics2001，170，2： | 1 |
| 14 | The lattic Boltzmann equation on irregular lattices显示文摘 | NANNELLI F SUCCI S | 1992 | Journal of Statistical Physics1992，68，3： | 1 |
| 15 | The prediction of heli- copter discrete frequency noise显示文摘 | FARASSANT F SUCCI G P | 1983 | Vertica1983，7，4： | 1 |
| 16 | Multiscale lattice Boltz-mann schemes with turbulence modeling显示文摘 | Filippova O Succi S Mazzocco F | 2001 | Journal ofComputational Physics2001，170，2： | 1 |
| 17 | β - Lactamase genes of the penicillin - susceptible Bacillus anthracis sterne strain 显示文摘 | Chen Y H Succi J Tenover F C | 2003 | Bacteriol2003，185，： | 1 |
| 18 | The prediction of helicopter rotor discrete frequency noise显示文摘 | Farassat F Succi G P | 1983 | Vertica1983，7，4： | 1 |
| 19 | Giant cell tumorof tendon sheath: study of 64 cases and review of literature显示文摘 | DI GRAZIA S SUCCI G FRAGETTA F | 2013 | GChir2013，34，56： | 1 |
| 20 | A Review of Propeller Discrete Frequency Noise Prediction Technology with Emphasis on two Current Methods for Time Domain Calculations 显示文摘 | Farassat F Succi G P | 1980 | Journal of Sound and Vibration1980，71，： | 1 |
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