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| 1 | Simultaneous removal of uranium and humic acid by cyclodextrin modified graphene oxide nanosheets显示文摘Cyclodextrin-modified graphene oxide nanosheets(denoted as CD/GO) were synthesized by an in-situ polymerization method and characterized by as well as Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy and potentiometric acid-base titration. The characterization results indicated that CD was successfully grafted onto GO surfaces by forming a chemical bond. Mutual effects on the simultaneous removal of hexavalent uranium and humic acid by CD/GO from aqueous solution were investigated. The results indicated that U(VI) and humic acid(HA) sorption on CD/GO were greatly affected by pH and ionic strength. The presence of HA enhanced U(VI) sorption at low pH and reduced U(VI) sorption at high pH, whereas the presence of U(VI) enhanced HA sorption. The surface adsorbed HA acted as a 'bridge' between U(VI) and CD/GO, and formed strong inner-sphere surface complexes with U(VI). Sorption isotherms of U(VI) or HA on CD/GO could be well fitted by the Langmuir model. This work highlights that CD/GO can be used as a promising material in the enrichment of U(VI) and HA from wastewater in U(VI) and humic substances obtained by environmental pollution cleanup. | SONG WenCheng SHAO DaDong LU SongSheng WANG XiangKe | 2014 | Science China Chemistry2014，57，9： | 12 |
| 2 | Neuronal tolerance to hypoxia-ischemia through recombinant adeno-associated viral vectors expressing neuronal and inducible nitric oxide synthase An in vivo study显示文摘BACKGROUND: Studies have confirmed that neuronal nitric oxide synthase (nNOS) mediates neurotoxic effects during the early stages of hypoxia-ischemia,while inducible nitric oxide synthase (iNOS) mediates delayed neurotoxicity during advanced stages of hypoxia-ischemia. OBJECTIVE: This study was designed to observe neuronal apoptosis and the expressions of nNOS,iNOS,p38 mitogen-activated protein kinase (MAPK),and caspase-3 mRNA following transfection of recombinant adeno-associated viral vectors separately expressing nNOS and iNOS antisense (rAAV-AsnNOS and rAVV-AsiNOS,respectively) into rat brains subjected to cerebral ischemia; to analyze mechanisms underlying elevated neuronal tolerance to hypoxia-ischemia. DESIGN: A randomized controlled in vivo experiment. SETTING: Fujian Institute of Neurosurgery & Department of Neurosurgery,Union Hospital,Fujian Medical University. MATERIALS: Eighty healthy adult male Sprague Dawley rats of clean grade were provided by the Zhejiang Laboratory Animal Center,China. The protocol was performed in accordance with ethical guidelines for the use and care of animals. The following vectors,rAAV-AsnNOS,rAAV-AsiNOS,and rAAV expressing the β-galactosidase gene (rAAV-LacZ),were successfully constructed by Fujian Institute of Neurosurgery. Rabbit anti-mouse nitrotyrosine (NT) monoclonal antibody (Zhongshan Jinqiao Biotechnology Co.,Ltd.,Beijing,China) and reverse transcription-polymerase chain reaction (RT-PCR) kit (two-step method) (Promega Company,USA) were used in this study. METHODS: This study was performed at the Fujian Institute of Neurosurgery in December 2003. Sixty rats were randomly divided into 3 groups,with 20 rats in each group: rAAV-AsnNOS group,rAAV-AsiNOS group,and rAAV-LacZ group. The remaining 20 rats served as controls. Pre-treated viral vectors (rAAV-AsnNOS,rAAV-AsiNOS,and rAAV-LacZ,respectively; each 50 μL,virus titer of 2×109 viral particles/mL) were transfected into the cerebral cortex of the targeted. Phosphate buffer saline (50 μL) was perfused identically into the rat cerebral cortex of the control group. All rats were subjected to cerebral ischemia by occluding the right middle cerebral artery with suture. Five time points (0,1,6,24,72 hours of ischemia,4 rats for each time point) were allotted to each group. Ischemic brain tissue specimens were prepared for index measurement. MAIN OUTCOME MEASURES: The percentage of NT-positive cells and apoptotic cells in the rat ischemic brain tissue specimens were measured by flow cytometry. The expressions of nNOS,iNOS,p38MAPK,and caspase-3 mRNA were measured by RT-PCR. RESULTS: During the early stages of ischemia (at 1 and 6 hours) with rAAV-AsnNOS transfection,the percentage of NT-positive cells,apoptotic cells,and the expressions of nNOS,p38MAPK and caspase-3 mRNA in the brain nerve cells were remarkably reduced compared to the control,rAAV-LacZ,and rAAV-AsiNOS groups. During the advanced stages of ischemia (at 24 and 72 hours) with rAAV-AsiNOS transfection,the above-mentioned indices were significantly reduced compared to control,rAAV-LacZ,and rAAV-AsnNOS groups. CONCLUSION: Following rAAV transfection,neuronal cells resisted ischemic injury in the MCAO ischemic rat model. Neurons transfected with rAAV-AsnNOS inhibited expression of nNOS,p38MAPK,and caspase-3 during the early stages of ischemia,and those transfected by rAAV-AsiNOS inhibited above-mentioned index expression during the advanced stages of ischemia. These results clearly demonstrate that neuronal apoptosis can be inhibited through the use of nNOS antisense. | Chunmei Chen Weizhong Yang Chunhua Wang Songsheng Shi Jianping Chen Yong Huang Dongsheng Cai | 2008 | Neural Regeneration Research2008，3，2： | 3 |
| 3 | CHARACTERIZATION AND CAUSATION OF RUNOFF AND SEDIMENT VARIATION IN THE JIALINGJIANG RIVER BASIN显示文摘The Jialingjiang River basin is one of the main sediment contributing areas in the upper reaches of the Changjiang River. Great changes have taken place in the runoff and sediment discharge in recent years. Comparing the data of 1991-2003 with the data of 1954-1990, the annual runoff of the Jialingjiang River basin decreased by 23 %, and the suspended sediment transport decreased by 74% or 105 million tons. The main factors affecting the reduction include a decrease in rainfall, sediment detention of hydraulic structures, soil and water conservation activities, sedimentation and sand dredging in the river channel. Thorough investigation and analysis of the contribution of each factor to the sediment decrease at Beibei Station was determined for the first time. The following are the contributing percentages for each factor: a decrease in runoff accounted for 32.9%; soil and water conservation measures accounted for 16.4%; sediment detention of hydraulic structures accounted for 30.5%; sedimentation, river channel sand dredging, and other factors accounted for 20.2%. These findings are very important for forecasting the trend of inflow sediment discharge variation. | Quanxi XU Songsheng CHEN Ming XIONG Shengwei LI Zefang CHEN | 2007 | International Journal of Sediment Research2007，22，3： | 2 |
| 4 | Molecular diagnosis and treatment of meningiomas:an expert consensus(2022)显示文摘Meningiomas are the most common primary intracranial neoplasm with diverse pathological types and complicated clinical manifestations.The fifth edition of the WHO Classification of Tumors of the Central Nervous System(WHO CNS5),published in 2021,introduces major changes that advance the role of molecular diagnostics in meningiomas.To follow the revision of WHO CNS5,this expert consensus statement was formed jointly by the Group of Neuro-Oncology,Society of Neurosurgery,Chinese Medical Association together with neuropathologists and evidence-based experts.The consensus provides reference points to integrate key biomarkers into stratification and clinical decision making for meningioma patients. | Jiaojao Deng Lingyang Hua Liuguan Bian Hong Chen Ligang Chen Hongwei Cheng Changwu Dou Dangmurenjiapu Geng Tao Hong Hongming Ji Yugang Jiang Qing Lan Gang Li Zhixiong Liu Songtao Qi Yan Qu Songsheng Shi Xiaochuan Sun Hajun Wang Yongping You Hualin Yu Shuyuan Yue Jianming Zhang Xiaohua Zhang Shuo Wang Ying Mao Ping Zhong Ye Gong Group of Neuro-Oncology Society of Neurosurgery Chinese Medical Association | 2022 | Chinese Medical Journal2022，135，16： | 1 |
| 5 | Effect of Magnetic Field on the Deposition of Transparent Diamond-Like Carbon (DLC) Films by RF-PCVD显示文摘 | HOU Huijun ZHU Xiagao LIN Songsheng | 2004 | Materials protection2004，37，9： | 1 |
| 6 | Evidence for tritium production in the Earth's interior显示文摘We have made a new investigation on the vertical profiles of tritium and helium isotopes in Lakes Van and Nemrut(Eastern Turkey),using experimental data from the reference by Kipfer et al.for study of long-term vertical mixing and deep water renewal in Lake Van.Lakes Van and Nemrut are crater lakes.Lake Nemrut is at the western border of Lake Van.The 3He and 4He are injected at the bottom of Lakes Van and Nemrut,and the both helium isotopes are confirmed from the mantle source.From 3H(tritium) data in Lakes Van and Nemrut,we have observed '3H anomaly' at the vertical profile of 3H concentrations in Lake Nemrut.The 3H concentration at the lake bottom is 10% higher than at the surface.The difference of 3H concentrations between surface and bottom is about 3.7±1.2 TU.This excess 3H should be injected from the lake bottom.An investigation on the origin of the injected tritium has been made.The results show the conventional origins are excluded,such as residence of precipitation tritium from nuclear testing in the early 1950s-1960s and tritium from known nuclear reactions.Based on the correlation of excess 3H with 3He and heat flow in Lake Nemrut,we infer that the 3He and 3H might be all from the mantle source,and produced by the supposed natural-nuclear-fusion,which might occur in an environment rich in water(H) and(U + Th) at high temperature and high pressure in the deep Earth.Detection of tritium in the Earth's interior is a key evidence for exploration of natural nuclear fusion in the deep Earth.Based on the published data,we have found that the excess 3He and 3H injected at the bottom of Lake Laacher(Germany) were also released from the mantle source.The present work will be helpful to the further study of mechanism of natural nuclear fusion in the Earth's interior. | JIANG SongSheng HE Ming | 2008 | Chinese Science Bulletin2008，53，4： | 1 |
| 7 | Over-expression of nitric oxide and nitric oxide synthase in the rat brain following focal ischemia显示文摘BACKGROUND: In cerebral ischemia,over-expression of nitric oxide (NO) exerts neurotoxic effects. OBJECTIVE: This study was designed to measure NO and nitric oxide synthase (NOS) expression in rat brain tissue during cerebral ischemia,and to correlate expression levels of NO and NOS with ischemic time. DESIGN: A complete randomized grouping design,controlled animal experiment. SETTING: Fujian Institute of Neurosurgery & Department of Neurosurgery,Union Hospital Affiliated Fujian Medical University. MATERIALS: This study was performed at the Fujian Institute of Neurosurgery in December 2003. Eighty healthy adult male Sprague Dawley (SD) rats of clean grade were provided by the Zhejiang Laboratory Animal Center. The protocol was performed in accordance with ethical guidelines for the use and care of animals. Kits for measuring NO expression by method of nitrate reductase (Boster Company,Wuhan,China) and NOS activity (Jiancheng Bioengineering Co.,Ltd.,Nanjing,China),as well as a kit for reverse transcription-PCR (RT-PCR,Promega,USA) were used in the present study. METHODS: Sixty-eight rats underwent cerebral ischemia by occluding the middle cerebral artery by suture method. Forty-eight successfully lesioned rats were included in the study. Six rats were used for each length of ischemic event (30 minutes,1,3,6,12,24,and 72 hours,and 5 days of ischemia). Additionally,a normal control group (n = 6,normally raised) and a sham-operated group (n = 6,corresponding cerebral vessels exposed,without occlusion of middle cerebral artery) were included in the analysis. MAIN OUTCOME MEASURES: Neurological function deficits were scored according to methods of Longa,Bederson,and colleagues. NO levels and NOS activity in the brain tissue were measured via nitrate reductase and chemical colorimetry,respectively. The expression of nitrotyrosine (NT),an in vivo specific metabolite of NO,was quantitatively measured by flow cytometry. Expression of endothelial,neuronal,and inducible NOS mRNA (eNOS,nNOS,and iNOS mRNA,respectively) in the cerebral ischemic region were semi-quantitatively analyzed by RT-PCR. RESULTS: Forty-eight ischemic rats and six sham-operated rats were analyzed. Neurological functional deficits increased with ischemic times (r = 0.765,P < 0.05). There were no significant differences in NO levels,NOS activity,and the percentage of NT-positive cells between the sham-operated and the normal control groups (P > 0.05). NO levels,NOS activity,and the percentage of NT-positive cells in brain tissue positively correlated to ischemia times (r = 0.932,0.914,0.924,respectively,P < 0.05). The percentage of NT-positive cells began to noticeably increase within 0.5 hour of after ischemia (9.50 %). NOS activity began to increase within 0.5 hour of ischemia and reached its peak level 3 days after ischemia. RT-PCR semi-quantitative analysis demonstrated that in the early stages of ischemia (0.5-6 hours),the expression of both eNOS and nNOS mRNA increased with ischemic time (t = 28.482-100.459,P < 0.01),while iNOS mRNA levels were almost undetectable. In the middle and advanced stages of ischemia (6 hours-5 days),iNOS mRNA levels were significantly increased compared to the control group (t = 36.742-82.058,P < 0.01); however,eNOS and nNOS mRNA levels were markedly reduced. CONCLUSION: With the prolongation of ischemic time,NO levels increased in cerebral tissue due to activation of various NOS. These measurements correlated with an increase in NT-positive cells and behavioral deficits. In the early stages of ischemia,eNOS and nNOS activities were increased,while in the later stage of ischemia,iNOS activity was increased and eNOS and nNOS activities were reduced. | Weizhong Yang Chunmei Chen Chunhua Wang Songsheng Shi Junrong Lei Yongliang Zhang | 2008 | Neural Regeneration Research2008，3，2： | 1 |
| 8 | Remeasurement of the half-life of 79 Se with the projectile X- ray detection method 显示文摘 | Jiang Songsheng He M Diao Lijun | 2001 | Chinese Physical Letters2001，18，6： | 1 |
| 9 | Measurement of the half-life of 79 Se with PX-AMS显示文摘 | HE Ming JIANG Songsheng JIANG Shan etal | 2002 | Nucl Instrum Methods B2002，194，： | 1 |
| 10 | Microcalorimetric study of Escherichia coli growth inhibited by the selenomorpholine complexes显示文摘 | Xi Li Yi Liu Ruming Zhao Jun Wu Xuesong Shen Songsheng Qu | 2000 | Biological Trace Element Research (-)2000，，1： | 1 |
| 11 | Interaction of U(VI) with Na-attapulgite in the presence and absence of humic acid as a function of pH, ionic strength and temperature显示文摘 | Xiaoping Song Yajie Wang Jingjing Cai Songsheng Lu Yunfei Chen | 2013 | Journal of Radioanalytical and Nuclear Chemistry2013，1，295： | 1 |
| 12 | Determination of tritium using a small Van de Graaff accelerator显示文摘 | Jiang Songsheng Yu Ansun Cui Yunfeng | 1984 | Nuclear Instruments and Methods in Physics Research Section B:Beam Interactions with Materials and Atoms1984，5，2： | 1 |
| 13 | Study on thermodynamic properties of a double salt,K2Mg(IO3)4 · 2H2O 显示文摘 | Wang Tianzhi Yu Yun Qu songsheng | 1999 | Thermochimica Acta1999，325，： | 1 |
| 14 | Kinetics of the action of three selenides on Staphylococcus aureus growth as studied by microcalorimetry显示文摘 | Huigang Liang Jun Wu Yi Liu Liping Yang Liming Hu Songsheng Qu | 2003 | Biological Trace Element Research2003，，2： | 1 |
| 15 | Microcalorimetric Studies of Rhus vernicifera LaccaseCatalytic Oxidation Reactions显示文摘 | X. Xiuyin W. Zhiyong W. Dingquan Q. Songsheng W. Cunxin | 2002 | Journal of Thermal Analysis and Calorimetry2002，，3： | 1 |
| 16 | The Action of the Selenomorpholine Compounds on Escherichia coli Growth by Microcalorimetry显示文摘 | Li Xi Liu Yi Wu Jun Liang Huigang Qu Songsheng | 2002 | Journal of Thermal Analysis and Calorimetry2002，，3： | 1 |
| 17 | Microcalorimetric study of Escherichia coli growth inhibited by the selenomorpholine complexes显示文摘 | Xi Li Yi Liu Ruming Zhao Jun Wu Xuesong Shen Songsheng Qu | 2000 | Biological Trace Element Research (-)2000，，1： | 1 |
| 18 | Preparation of YAG:Ce3+ phosphor by sol-gel low temperature combustion method and its luminescent properties显示文摘 | Yan Xinghuang Zheng Songsheng Yu Ruimin | 2008 | Transcactions of Nonferrous Metals Society of China2008，18，： | 1 |
| 19 | Microcalorimetric Study of Bacterial Growth 显示文摘 | Xie Changli Tang Hnokuan Qu Songsheng | 1988 | Thermochim Acta1988，，123： | 1 |
| 20 | Determination of the half-life ^79 Se with the accelerator mass spectrometry technique 显示文摘 | JIANG Songsheng GUO Jingru JIANG Shan | 1997 | Nucl Instrum Methods Phys Res Sect B1997，123，： | 1 |
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