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| 1 | Hepatorenal syndrome显示文摘Hepatorenal syndrome (HRS) is a 'functional' and reversible form of renal failure that occurs in patients with advanced chronic liver disease. The distinctive hallmark feature of HRS is the intense renal vasoconstriction caused by interactions between systemic and portal hemodynamics. This results in activation of vasoconstrictors and suppression of vasodilators in the renal circulation. Epidemiology, pathophysiology, as well as current and emerging therapies of HRS are discussed in this review. | Sharon Turban Paul J Thuluvath Mohamed G Atta | 2007 | World Journal of Gastroenterology2007，13，30： | 14 |
| 2 | 多元PCR在黑鲍(Haliotis rubra)微卫星遗传研究中的应用显示文摘根据D03等11个微卫星引物单位点PCR扩增时优化的扩增条件(退火温度、MgCl2等)和11个微卫星引物所带有的荧光颜色、等位基因大小变异范围及反应的灵敏度,可将11个微卫星引物分成A组(D03、G04、B04和H08)、B组(A09、H11、A08和G01)和C组(CmrHr1.24、CmrHr2.30和CmrHr2.23)进行多元PCR扩增,结果显示各组内位点之间的分离清晰,这表明多元PCR可以大大提高微卫星检测仪器的使用效率和提高实验效果,在微卫星研究中是一种快速便捷的方法。 | 黎中宝 Appleyard Sharon A Elliott Nicholas G | 2005 | 海洋与湖沼2005，36，4： | 8 |
| 3 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 4 | Regulatory challenges for new drugs to treat obesity and eomorbid metabolic disorders 显示文摘 | David J H Jane G Sharon L S | 2009 | Br J ClinPharmaeol2009，68，6： | 1 |
| 5 | The effect of informed consent on stress levels associated with extraction of impacted mandibular third molars显示文摘 | Casap N Alterman M Sharon G | 2008 | J Oral Maxiilofac Surg2008，66，5： | 1 |
| 6 | Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders显示文摘 | Sharon G Hyde ER McCue T | 2013 | Cell2013，155，7： | 1 |
| 7 | Feet First:Diabetes Care显示文摘 | Joan E Halpin L Sharon G | 1999 | American Journal of Nursing1999，99，2： | 1 |
| 8 | Mutation detection in the ABCC6 gene and genotype-phenotype analysis in a large international case series affected by pseudoxanthoma elasticum显示文摘 | Ellen G Olivier V Sharon F | 2007 | J Med Genet2007，5，6： | 1 |
| 9 | Practice parameters for the manage- ment of hemorrhoids显示文摘 | Peter C Neal C Sharon G | 2005 | Dis Colon Rectum2005，48，： | 1 |
| 10 | Research Productivity Over the Life Cycle:Evidence for Academic Scientists显示文摘 | Sharon G and Paula E Stephan | 1991 | American Economic Review1991，，： | 1 |
| 11 | Regeneration of blueberry plantlets from leaf segment显示文摘 | SHARON G B CHEE K CHIN C | 1988 | Hortseience1988，23，4： | 1 |
| 12 | Mechanisms of rotenoneinduced proteasome inhibition显示文摘 | Arthur P C Sharon L Arthur G | | 0，，04： | 1 |
| 13 | Regeneration of blueberry plantlets from leaf segments显示文摘 | Sharon C B Chin C K Jelenkovic G | 1988 | HortScience1988，23，4： | 1 |
| 14 | Crack Front Waves in Dynamic Fracture显示文摘 | Fineberg J Sharon E Cohen G | 2003 | International Journal of Fracture2003，119，： | 1 |
| 15 | Specialized metabolites from the microbiome in health and disease显示文摘 | Sharon G Garg N Debelius J | 2014 | Cell Metab2014，20，5： | 1 |
| 16 | Outbreaks of clinical mastitis caused by Trichosporon beigelii in dairy herds 显示文摘 | Ruben N G David J W Sharon A S | 2001 | J Am Vet Med Assoc2001，218，2： | 1 |
| 17 | Correlates of high-risk sexual behavior among young men who have sex with men显示文摘 | Michael G Dudley Sharon Scales Rostosky Bethe A Korfhage | 2004 | AIDS Education and Prevention2004，16，4： | 1 |
| 18 | Research productivity over the life cycle: Evidence for academic scientists显示文摘 | LEVIN SHARON G STEPHAN PAULA E | 1991 | The American Economic Review1991，81，1： | 1 |
| 19 | Medulloblastoma in childhood: revisiting intrathecal therapy in infants and children 显示文摘 | Sharon C Martin G Michael V | 2010 | Cancer Chemother Pharmacol2010，6，65： | 1 |
| 20 | Adoption of Environmental Innova- tions: Analysis from the Waipara Wine Industry 显示文摘 | Sharon L F Ross C Rachel G | 2013 | Wine Economies and Policy2013，2，1： | 1 |
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