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| 1 | AGC1.5 Kinase Phosphorylates RopGEFs to Control Pollen Tube Growth显示文摘在被子植物的两倍授精通过花粉试管要求无法移动的精子的指向的交货到卵。成长尖端的花粉试管的极性与顶端的血浆膜通过植物(ROP-GTP ) 的活跃 Rho GTPases 的动态协会被维持。为 ROP (RopGEFs ) 的 Guanine 核苷酸交换因素催化 ROP 的激活并且从而影响空间与时间的 ROP 发信号。而 RopGEFs 被发现了是 phosphorylated 蛋白质,为他们的 phosphorylation 在负责的 kinases ? vivo 和在花粉试管生长的 RopGEF phosphorylation 的生物后果仍然保持不清楚。我们这里报导细胞质的 kinases 的 Arabidopsis AGC1.5 亚科在花粉试管生长期间为 ROP-GTP 的限制本地化是批评的。AGC1.5 和 AGC1.7 功能的损失在花粉试管发信号的 ROP 下游地导致了活跃 ROP 和有缺点的事件的错误目标。AGC1.5 在容易的领域的保存重量的单位残余经由他们的催化容易的领域和 phosphorylates RopGEFs 与 RopGEFs 交往。AGC1.5 和 AGC1.7 功能的损失在花粉试管导致了 RopGEFs 的错误目标,类似于由 AGC1.5 显示 RopGEFs non-phosphorylatable 的变化引起的显型。一起,我们的结果在花粉试管的极的生长期间提供机械学的卓见进 ROP 的空间与时间的激活。 | En Li Yong Cui Fu-Rong Ge Sen Chai Wei-Tong Zhang Qiang-Nan Feng Liwen Jiang Sha Li Yan Zhang | 2018 | Molecular Plant2018，11，9： | 5 |
| 2 | S-acylation of CBL10/SCaBP8 by PAT10 is crucial for its tonoplast association and function in salt tolerance显示文摘Protein S-acylation is a reversible posttranslational modification that involves the addition of a 16-carbon saturated lipid group,usually a palmitate group,to cysteine residues of a substrate protein(Hemsley and Grierson 2008;Hemsley et al.2013;Running 2014). | Sen Chai Fu-Rong Ge Yan Zhang Sha Li | 2020 | Journal of Integrative Plant Biology2020，62，6： | 4 |
| 3 | The journey to glory: receptor-like kinases in pollen tube growth显示文摘Double fertilization in angiosperm is a prerequisite for seed and fruit development and has been under close scrutiny in the past decades. Communications between male and female gametophytes are especially important in achieving successful reproduction, in which receptor-like kinases are dominant players. Here, we review progress on the functionality of receptor-like kinases(RLKs) in pollen tube growth and point out challenges facing the study of RLKs in pollen biology. | Sen Chai Furong Ge Sha Li Yan Zhang | 2016 | Science Bulletin2016，61，11： | 4 |
| 4 | Sugars promote graft union development in the heterograft of cucumber onto pumpkin显示文摘The use of heterografts is widely applied for the production of several important commercial crops,but the molecular mechanism of graft union formation remains poorly understood.Here,cucumber grafted onto pumpkin was used to study graft union development,and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated.Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection,and the scion showed evident callus production compared with the rootstock 3 days after grafting.Consistently,transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development,the cell cycle,and sugar metabolism during both vascular reconnection and healing,indicating distinct mechanisms.Additionally,lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection.Next,we found that the healing process of grafted etiolated seedlings was significantly delayed,and graft success,xylem reconnection,and the growth of grafted plants were enhanced by exogenous glucose.This demonstrates that graft union formation requires the correct sugar content.Furthermore,we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin(TOR)inhibitor AZD-8055,and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment.Taken together,our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future. | Li Miao Qing Li Tian-shu Sun Sen Chai Changlin Wang Longqiang Bai Mintao Sun Yansu Li Xing Qin Zhonghua Zhang Xianchang Yu | 2021 | Horticulture Research2021，8，1： | 3 |
| 5 | Targeting and signaling of Rho of plants guanosine triphosphatases require synergistic interaction between guanine nucleotide inhibitor and vesicular trafficking显示文摘Most eukaryotic cells are polarized.Common toolbox regulating cell polarization includes Rho gua-nosine triphosphatases(GTPases),in which spatio-temporal activation is regulated by a plethora of reg-ulators.Rho of plants(ROPs)are the only Rho GTPases in plants.Although vesicular trafficking was hinted in the regulation of ROPs,it was unclear where vesicle-carried ROP starts,whether it is dynamically regulated,and which components participate in vesicle-mediated ROP targeting.In addition,although vesicle trafficking and guanine nucleotide inhibitor(GDI)pathways in Rho signaling have been extensively studied in yeast,it is unknown whether the two pathways interplay.Unclear are also cellular and developmental con-sequences of their interaction in multicellular organisms.Here,we show that the dynamic targeting of ROP through vesicles requires coat protein complexⅡand ADP-ribosylation factor 1-mediated post-Golgi trafficking.Trafficking of vesicle-carried ROPs between the plasma membrane and the trans-Golgi network is mediated through adaptor protein 1 and sterol-mediated endocytosis.Finally,we show that GDI and vesicle trafficking synergistically regulate cell polar-ization and ROP targeting,suggesting that the estab-lishment and maintenance of cell polarity is regulated by an evolutionarily conserved mechanism. | Fu-Rong Ge Sen Chai Sha Li Yan Zhang | 2020 | Journal of Integrative Plant Biology2020，62，10： | 2 |
| 6 | A novel tomato MYC-type ICE1-like transcription factor, SlICE1a , confers cold, osmotic and salt tolerance in transgenic tobacco显示文摘 | Hai-Long Feng Na-Na Ma Xia Meng Song Zhang Jie-Ru Wang Sen Chai Qing-Wei Meng | 2013 | Plant Physiology and Biochemistry2013，，： | 1 |
| 7 | Transcriptome Profiling of Abscisic Acid-Related Pathways in SNAC4/9-Silenced Tomato Fruits显示文摘The NAC(NAM,ATAF,and CUC)family is considered one of the largest families of plant transcription factor,and its members are involved in fruit ripening.Abscisic acid(ABA)has been demonstrated to modulate the fruit ripening process.By applying the virus-induced gene silencing method and next-generation sequencing technology,we conducted a compara-tive analysis of the eff ects of SNAC4(SlNAC48,accession number:NM 001279348.2)and SNAC9(SlNAC19,accession number:XM 004236996.2)on tomato fruit ripening.The results of high-throughput sequencing identified 1262 significant(p<0.05)diff erentially expressed genes(DEGs)in SNAC4-silenced fruit compared to control fruit,while 655 DEGs were identified in SNAC9-silenced fruit.In addition,we selected 26 and 30 significant DEGs(p<0.05 and log 2-fold change>1.0)related to ABA in SNAC4-silenced and SNAC9-silenced tomatoes,respectively,for further analysis.The XET gene and two other genes(E8 and EXP1)were significantly down and upregulated in SNAC4-silenced tomatoes,respectively.However,the PYL9 gene and four other genes(PP2C,CYP707A2,EXPA6,and ACS6)were significantly down and upregulated in SNAC9-silenced tomatoes,respectively.In addition,ten DEGs were selected for use in tests to confirm the accuracy of the transcriptomic results by quantitative real-time polymerase chain reaction(qRT-PCR).Our results highlight the relationship between SNAC4/9 and ABA in the regulation of tomato ripening,which may help provide a theoretical basis for further research on the mechanisms of tomato fruit ripening and senescence. | Yefang Liu Yanan Zhao Liping Chai Jiaqian Zhou Sen Yang Xiaohong Kou Zhaohui Xue | 2021 | Transactions of Tianjin University2021，27，6： | 0 |
| 8 | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon显示文摘Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea(CPPU),an exogenous cytokinin(CK)growth regulator,is the conventional method for inducing fruit set during melon(Cucumis melo L.)production;however,the mechanism by which CPPU induces fruit set is unclear.Through histological and morphological observations,fruit size was comparable between CPPU-induced fruits and normal pollinated fruits because CPPU-induced fruits had higher cell density but smaller cell size compared with normal pollinated fruits.CPPU promotes the accumulation of gibberellin(GA)and auxin and decreases the level of abscisic acid(ABA)during fruit set.Moreover,application of the GA inhibitor paclobutrazol(PAC)partially inhibits CPPU-induced fruit set.Transcriptome analysis revealed that CPPU-induced fruit set specifically induced the GA-related pathway,in which the key synthase encoding gibberellin 20-oxidase 1(CmGA20ox1)was specifically upregulated.Further study indicated that the two-component response regulator 2(CmRR2)of the cytokinin signaling pathway,which is highly expressed at fruit setting,positively regulates the expression of CmGA20ox1.Collectively,our study determined that CPPU-induced melon fruit set is dependent on GA biosynthesis,providing a theoretical basis for the creation of parthenocarpic melon germplasm. | Yue Liu Yang Li Huixin Guo Bingsheng Lv Jing Feng Huihui Wang Zhonghua Zhang Sen Chai | 2023 | Horticulture Research2023，10，6： | 0 |
| 9 | A novel mutation in ACS11 leads to androecy in cucumber显示文摘Sex determination in plants gives rise to unisexual flowers. A better understanding of the regulatory mechanism underlying the production of unisexual flowers will help to clarify the process of sex determination in plants and allow researchers and farmers to harness heterosis. Androecious cucumber(Cucumis sativus L.) plants can be used as the male parent when planted alongside a gynoecious line to produce heterozygous seeds, thus reducing the cost of seed production. The isolation and characterization of additional androecious genotypes in varied backgrounds will increase the pool of available germplasm for breeding. Here, we discovered an androecious mutant in a previously generated ethyl methanesulfonate(EMS)-mutagenized library of the cucumber inbred line ‘406’. Genetic analysis, whole-genome resequencing, and molecular marker-assisted verification demonstrated that a nonsynonymous mutation in the ethylene biosynthetic gene 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 11(ACS11) conferred androecy. The mutation caused an amino acid change from serine(Ser) to phenylalanine(Phe) at position 301(S301F). In vitro enzyme activity assays revealed that this S301F mutation leads to a complete loss of enzymatic activity. This study provides a new germplasm for use in cucumber breeding as the androecious male parent, and it offers new insights into the catalytic mechanism of ACS enzymes. | WANG Jie LI Shuai CHEN Chen ZHANG Qi-qi ZHANG Hui-min CUI Qing-zhi CAI Guang-hua ZHANG Xiao-peng CHAI Sen WAN Li YANG Xue-yong ZHANG Zhong-hua HUANG San-wen CHEN Hui-ming SUN Jin-jing | 2023 | Journal of Integrative Agriculture2023，22，11： | 0 |
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