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| 1 | Golgi protein-73:A biomarker for assessing cirrhosis and prognosis of liver disease patients显示文摘BACKGROUND Reliable biomarkers of cirrhosis,hepatocellular carcinoma(HCC),or progression of chronic liver diseases are missing.In this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transmembrane protein expressed in epithelial cells.As a result,GP73 expression was found primarily in biliary epithelial cells,with only slight detection in hepatocytes.However,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in hepatocytes.So far,few studies have assessed GP73 as a diagnostic or prognostic marker of liver fibrosis and disease progression.AIM To assess serum GP73 efficacy as a diagnostic marker of cirrhosis and/or HCC or as predictor of liver disease progression.METHODS GP73 serum levels were retrospectively determined by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,Research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from two tertiary Academic centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate aminotransferase(AST)/Platelets(PLT)ratio index(APRI)was also calculated at the relevant time points in all patients.Two hundred and three patients had chronic hepatitis B,183 chronic hepatitis C,198 alcoholic liver disease,28 autoimmune cholestatic liver diseases,15 autoimmune hepatitis,and 5 with other liver-related disorders.The duration of follow-up was 50(57)mo[median(interquartile range)].The development of cirrhosis,liver decompensation and/or HCC during follow-up were assessed according to internationally accepted guidelines.In particular,the surveillance for the development of HCC was performed regularly with ultrasound imaging and alpha-fetoprotein(AFP)determination every 6 mo in cirrhotic and every 12 mo in non-cirrhotic patients.RESULTS Increased serum levels of GP73(>20 units)were detected at initial evaluation in 277 out of 632 patients(43.8%).GP73-seropositivity correlated at baseline with the presence of cirrhosis(96.4%vs 51.5%,P<0.001),decompensation of cirrhosis(60.3%vs 35.5%,P<0.001),presence of HCC(18.4%vs 7.9%,P<0.001)and advanced HCC stage(52.9%vs 14.8%,P=0.002).GP73 had higher diagnostic accuracy for the presence of cirrhosis compared to APRI score[Area under the curve(AUC)(95%CI):0.909(0.885-0.934)vs 0.849(0.813-0.886),P=0.003].Combination of GP73 with APRI improved further the accuracy(AUC:0.925)compared to GP73(AUC:0.909,P=0.005)or APRI alone(AUC:0.849,P<0.001).GP73 levels were significantly higher in HCC patients compared to non-HCC[22.5(29.2)vs 16(20.3)units,P<0.001)and positively associated with BCLC stage[stage 0:13.9(10.8);stage A:17.1(16.8);stage B:19.6(22.3);stage C:32.2(30.8);stage D:45.3(86.6)units,P<0.001]and tumor dimensions[very early:13.9(10.8);intermediate:19.6(18.4);advanced:29.1(33.6)units,P=0.004].However,the discriminative ability for HCC diagnosis was relatively low[AUC(95%CI):0.623(0.570-0.675)].Kaplan-Meier analysis showed that the detection of GP73 in patients with compensated cirrhosis at baseline,was prognostic of higher rates of decompensation(P=0.036),HCC development(P=0.08),and liver-related deaths(P<0.001)during follow-up.CONCLUSION GP73 alone appears efficient for detecting cirrhosis and superior to APRI determination.In combination with APRI,its diagnostic performance can be further improved.Most importantly,the simple GP73 measurement proved promising for predicting a worse outcome of patients with both viral and nonviral chronic liver diseases. | Nikolaos K Gatselis Tamás Tornai Zakera Shums Kalliopi Zachou Asterios Saitis Stella Gabeta Roger Albesa Gary L Norman Mária Papp George N Dalekos | 2020 | World Journal of Gastroenterology2020，26，34： | 21 |
| 2 | An integrated approach for increasing breeding efficiency in apple and peach in Europe显示文摘Despite the availability of whole genome sequences of apple and peach,there has been a considerable gap between genomics and breeding.To bridge the gap,the European Union funded the FruitBreedomics project(March 2011 to August 2015)involving 28 research institutes and private companies.Three complementary approaches were pursued:(i)tool and software development,(ii)deciphering genetic control of main horticultural traits taking into account allelic diversity and(iii)developing plant materials,tools and methodologies for breeders.Decisive breakthroughs were made including the making available of ready-to-go DNA diagnostic tests for Marker Assisted Breeding,development of new,dense SNP arrays in apple and peach,new phenotypic methods for some complex traits,software for gene/QTL discovery on breeding germplasm via Pedigree Based Analysis(PBA).This resulted in the discovery of highly predictive molecular markers for traits of horticultural interest via PBA and via Genome Wide Association Studies(GWAS)on several European genebank collections.FruitBreedomics also developed pre-breeding plant materials in which multiple sources of resistance were pyramided and software that can support breeders in their selection activities.Through FruitBreedomics,significant progresses were made in the field of apple and peach breeding,genetics,genomics and bioinformatics of which advantage will be made by breeders,germplasm curators and scientists.A major part of the data collected during the project has been stored in the FruitBreedomics database and has been made available to the public.This review covers the scientific discoveries made in this major endeavour,and perspective in the apple and peach breeding and genomics in Europe and beyond. | Francois Laurens Maria JoséAranzana Pere Arus Daniele Bassi Marco Bink Joan Bonany Andrea Caprera Luca Corelli-Grappadelli Evelyne Costes Charles-Eric Durel Jehan-Baptiste Mauroux Hélène Muranty Nelson Nazzicari Thierry Pascal Andrea Patocchi Andreas Peil Bénédicte Quilot-Turion Laura Rossini Alessandra Stella Michela Troggio Riccardo Velasco Eric van de Weg | 2018 | Horticulture Research2018，5，1： | 10 |
| 3 | Hepatitis B virus reactivation in hepatitis B virus surface antigen negative patients receiving immunosuppression: A hidden threat显示文摘AIM: To present the characteristics and the course of a series of anti- hepatitis B virus core antibody (HBc) antibody positive patients, who experienced hepatitis B virus (HBV) reactivation after immunosuppression. METHODS: We retrospectively evaluated in our tertiary centers the medical records of hepatitis B virus surface antigen (HBsAg) negative patients who suffered from HBV reactivation after chemotherapy or immunosuppression during a 3-year period (2009-2011). Accordingly, the clinical, laboratory and virological characteristics of 10 anti-HBc (+) anti-HBs (-)/HBsAg (-) and 4 anti-HBc (+)/antiHBs (+)/HBsAg (-) patients, who developed HBV reactivation after the initiation of chemotherapy or immunosuppressive treatment were analyzed. Quantitative determination of HBV DNA during reactivation was performed in all cases by a quantitative real time polymerase chain reaction kit (COBAS Taqman HBV Test; cut-off of detection: 6 IU/mL). RESULTS: Twelve out of 14 patients were males; median age 74.5 years. In 71.4% of them the primary diagnosis was hematologic malignancy; 78.6% had received rituximab (R) as part of the immunosuppressive regimen. The median time from last chemotherapy schedule till HBV reactivation for 10 out of 11 patients who received R was 3 (range 2-17) mo. Three patients (21.4%) deteriorated, manifesting ascites and hepatic encephalopathy and 2 (14.3%) of them died due to liver failure. CONCLUSION: HBsAg-negative anti-HBc antibody positive patients can develop HBV reactivation even 2 years after stopping immunosuppression, whereas prompt antiviral treatment on diagnosis of reactivation can be lifesaving. | Kalliopi Zachou Alexandros Sarantopoulos Nikolaos K Gatselis Themistoklis Vassiliadis Stella Gabeta Aggelos Stefos Asterios Saitis Panagiota Boura George N Dalekos | 2013 | World Journal of Hepatology2013，5，7： | 6 |
| 4 | Primary biliary cirrhosis-specific autoantibodies in first degree relatives of Greek primary biliary cirrhosis patients显示文摘AIM:To determine the prevalence and significance of primary biliary cirrhosis (PBC)-specific autoantibodies in firstdegree relatives (FDRs) of Greek PBC patients. METHODS:The presence of antimitochondrial antibodies (AMA) and PBCspecific antinuclear antibodies (ANA) were determined using indirect immunofluores-cence assays, dot-blot assays, and molecularly based enzyme-linked immunosorbent assays in 101 asymp-tomatic for liver-related symptoms FDRs of 44 PBCpatients. In order to specify our results, the same investigation was performed in 40 healthy controls and in a disease control group consisting of 40 asymptomatic for liver-related symptoms FDRs of patients with other autoimmune liver diseases namely, autoimmune hepati-tis-1 or primary sclerosing cholangitis (AIH-1/PSC). RESULTS: AMA positivity was observed in 19 (only 4 with abnormal liver function tests) FDRs of PBC patients and none of the healthy controls. The preva-lence of AMA was significantly higher in FDRs of PBC patients than in AIH-1/PSC FDRs and healthy controls [18.8%, 95% confidence interval (CI):12%-28.1% vs 2.5%, 95% CI:0.1%-14.7%, P = 0.01; 18.8%, 95% CI:12%-28.1% vs 0%, 95% CI: 0%-10.9%, P = 0.003, respectively]. PBC-specific ANA positivity was observed in only one FDR from a PSC patient. Multivariate analysis showed that having a proband with PBC independently associated with AMA positivity (odds ratio: 11.24, 95% CI:1.27-25.34, P = 0.03) whereas among the investigated comorbidities and risk factors, a positive past history for urinary tract infections (UTI) was also independently associated with AMA detection in FDRs of PBC patients (odds ratio:3.92, 95% CI:1.25-12.35,P = 0.02). CONCLUSION:In FDRs of Greek PBC patients, AMA prevalence is significantly increased and independently associated with past UTI. PBC-specific ANA were not detected in anyone of PBC FDRs. | Theodoros A Zografos Nikolaos Gatselis Kalliopi Zachou Christos Liaskos Stella Gabeta George K Koukoulis George N Dalekos | 2012 | World Journal of Gastroenterology2012，18，34： | 4 |
| 5 | Ag-Zn粉末的机械合金化和随后的热处理(英文)显示文摘研究了在机械合金化过程中Ag和Zn以及随后的热处理中的显微组织演变。机械合金化在SPEX8000D铣床中进行。采用X射线衍射(XRD)﹑透射电子显微镜(TEM)和扫描电子显微镜(SEM)观察样品的显微组织。使用差示扫描量热计(DSC)研究样品的热性能。结果表明,在合金化初期的碾磨过程中能观察到ε,β,β′,α固溶体和剩余的Zn。随后,ε,β,β′和Zn相消失,α固溶体中的Zn浓度剧烈升高。在碾磨7.2ks后,机械合金化过程达到稳定状态。在此期间,α固溶体的组分和晶粒尺寸保持不变。后续热处理表明α固溶体也能够通过机械合金化结合热处理得到。最后,为了得到α固溶体,根据碾磨和热处理过程中样品的微观组织演变,提出一个最佳的工艺路线。 | Danny GUZMN Oscar RIVERA Claudio AGUILAR Stella ORDOEZ Carola MARTíNEZ Daniel SERAFINI Paula ROJAS | 2013 | Transactions of Nonferrous Metals Society of China2013，23，7： | 2 |
| 6 | Serratia marcescens quinoprotein glucose dehydrogenase activity mediates medium aciditication and inhibition of prodigiosin production by glucose显示文摘 | JFENDER J E BENDER C M STELLA N A | 2012 | Applied and Environmental Microbology2012，78，17： | 1 |
| 7 | Endovaseular treatment of common carotid artery dissection via the superficial temporal artery显示文摘 | Stella N Palombo G Filippi F | 2010 | J Endovasc Ther2010，17，4： | 1 |
| 8 | Simultaneotrs urokinase perfusion in renal artery and vein in a case of renal vein thrombosis显示文摘 | Stella N Rolli A Catalano A | 2001 | Minerva Cardioangiol2001，49，4： | 1 |
| 9 | Function-al role of high - affinity anandamide transport, as re- vealed by selective inhibition 显示文摘 | Beltramo M Stella N Calignano A | 1997 | Science1997，277，5329： | 1 |
| 10 | Protective effect of the inhibition of the renin-angiotensin system on aging显示文摘 | Basso N Pag lia N Stella I | 2005 | Regul Pept2005，128，： | 1 |
| 11 | Cannabinoid CB1 receptors and ligands in vertebrate retina:localization and function of an endogenous signaling system显示文摘 | Straiker A Stella N Piomelli D | 1999 | Proc Natl Acad Sci USA1999，96，14： | 1 |
| 12 | Endovascular treatment of common carotid artery dissection via the superficial temporal artery显示文摘 | Stella N Palombo G Filippi F | 2010 | J Endovasc Ther2010，17，4： | 1 |
| 13 | Influence of Admission Glucose Level on Long-Term Prognosis in Patients With Acute Coronary Syndrome显示文摘 | Stella M. Macín Eduardo R. Perna María L. Coronel Jorge O. Kriskovich Pablo A. Bayol Valeria A. Franciosi Jorge L. Riera-Stival Bilda González-Arjol Jorge R. Badaracco | 2006 | 2006，，： | 1 |
| 14 | Simultaneous urokinase perfusion in renal artery and vein in a case of renal vein thrombosis 显示文摘 | Stella N Rolli A Catalano A | 2001 | Minerva Cardioangiol2001，49，4： | 1 |
| 15 | A second endogenous cannabinoid that modulates long-term potentiation显示文摘 | Stella N Schweitzer P PiomeUl D | 1997 | Nature1997，388，6644： | 1 |
| 16 | ATP induces a rapid and pronounced increase in 2-arachidonoylglycerol production by astrocytes, a response limited by monoacylglycerol lipase显示文摘 | Walter L Dinh T Stella N | 2004 | J Neurosci2004，24，37： | 1 |
| 17 | Endovascular treatment of common carotid artery dissection via the super- ficial temporal artery 显示文摘 | STELLA N PALOMBO G FILIPPI F | 2010 | J Endovasc Ther2010，17，4： | 1 |
| 18 | Safety and effectiveness of combining carotid artery stenting with cardiac surgery: preliminary results of a single-center experience 显示文摘 | Palombo G Stella N Faraglia V | 2009 | J Cardiovasc Surg (Torino)2009，50，： | 1 |
| 19 | A High quality draft consensus sequence of the genome of a heterozygous grapevine variety显示文摘 | VELASCO R ZHARKIKH A TROGGIO M CARTWRIGHT DA CESTARO A PRUSS D PINDO M FITZGERALD LM VEZZULLI S REID J MALACARNE G ILIEV D COPPOLA G WARDELL B MICHELETTI D MACALMA T FACCI M MITCHELL JT PERAZZOLLI M ELDREDGE G GATTO P OYZERSKI R MORETTO M GUTIN N STEFANINI M CHEN Y SEGALA C DAVENPORT C DEMATYTE L MRAZ A BAT- TILANA J STORMO K COSTA F TAO Q SI-AMMOUR A HARKINS T LACKEY A PERBOST C TAILLON B STELLA A SOLOVYEV V FAWCETY JA STERCK L VANDEPOELE K GRANDO SM TOPPO S MOSER C LANCHBURY J BOGDEN R SKOLNICK M SGARAMELLA V BHATNAGAR SK FONTANA P GUTIN A VAN DE PEER Y SALAMINI F VIOLA R | 2007 | PLoS One2007，2，： | 1 |
| 20 | Model simplification and development via reuse, sensitivity analysis and composition: A case study in crop modeling显示文摘 | Stella T Frasso N Negrini G | 2014 | Environmental Modelling & Software2014，，59： | 1 |
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