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| 1 | Applications of Three DNA Barcodes in Assorting Intertidal Red Macroalgal Flora in Qingdao, China显示文摘This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI (cytochrome oxidase subunitⅠ) are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA (universal plastid amplicon, domain V of 23S rRNA), and ITS (nuclear internal transcribed spacer) were employed to analyze common species of intertidal red seaweeds in Qingdao (119.3 -121 E, 35.35 -37.09 N). The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification. | ZHAO Xiaobo PANG Shaojun SHAN Tifeng LIU Feng | 2013 | Journal of Ocean University of China2013，12，1： | 7 |
| 2 | Temperature and light tolerance of representative brown,green and red algae in tumble culture revealed by chlorophyll fluorescence measurements显示文摘Laminaria japonica,Undaria pinnatifida,Ulva lactuca,Grateloupia turuturu and Palmaria palmata are suitable species that fit the requirements of a seaweed-animal integrated aquaculture system in terms of their viable biomass,rapid growth and promising nutrient uptake rates.In this investigation,the responses of the optimal chlorophyll fluorescence yield of the five algal species in tumble culture were assessed at a temperature range of 10~30℃.The results revealed that Ulva lactuca was the most resistant species to high temperature,withstanding 30℃ for 4 h without apparent decline in the optimal chlorophyll fluorescence yield.While the arctic alga Palmaria palmata was the most vulnerable one,showing significant decline in the optimal chlorophyll fluorescence yield at 25℃ for 2 h.The cold-water species Laminaria japonica,however,demonstrated strong ability to cope with higher temperature(24~26℃)for shorter time(within 24 h)without significant decline in the optimal chlorophyll fluorescence yield.Grateloupia turuturu showed a general decrease in the optimal chlorophyll fluorescence yield with the rising temperature from 23 to 30℃,similar to the temperate kelp Undaria pinnatifida.Changes of chlorophyll fluorescence yields of these algae were characterized differently indicating the existence of species-unique strategy to cope with high light.Measurements of the optimal chlorophyll fluorescence yield after short exposure to direct solar irradiance revealed how long these exposures could be without significant photoinhibition or with promising recovery in photosynthetic activities.Seasonal pattern of alternation of algal species in tank culture in the Northern Hemisphere at the latitude of 36°N was proposed according to these basic measurements. | PANG Shaojun SHAN Tifeng | 2008 | Acta Oceanologica Sinica2008，27，4： | 4 |
| 3 | Zoospores of Undaria pinnatifida:their efficiency to attach under different water velocities and conjugation behavior during attachment显示文摘In the invading course of Undaria pinnatifida, zoospore attachment in a dynamically changed subtidal water environment is crucial for the establishment of a potential population in alien waters. Among many abiotic factors that may interfere with the attachment process, water velocity is the most important one. In this investigation, the effect of water velocity on zoospore attachment of U. pinnatifida was investigated in an artificially designed system. It was found that freshly released zoospores that were transported by water flowing at 0~16 cm/s showed no difficulty in attaching the smooth surface. Zoospore attachment decreased at elevated water flowing rates. At 70 cm/s no spore attachment occurred. Spores that have settled on glass slide for up to 1 h could not be stripped away by flowing water at a rate of 129 cm/s, the same was true of the 20 d old filamentous gametophytes. It was found that more than 70% of free-swimming zoospores tended to settle down adjacent to the settled spores and formed conjugated clusters from two up to a few hundred cells in still culture. | PANG Shaojun SHAN Tifeng | 2008 | Acta Oceanologica Sinica2008，27，6： | 1 |
| 4 | Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature显示文摘 | PANG SHAOJUN LIU FENG SHAN TIFENG | 2009 | J Appl Phycol2009，21，： | 1 |
| 5 | Potential of Ulva prolifera in phytoremediation of seawater polluted by cesium and cobalt: an experimental study on the biosorption and kinetics显示文摘Radionuclides accidentally released from nuclear power plants,such as ^(137)Cs and ^(60)Co,can lead to severe contamination of marine ecosystems.Living macroalgae are effi cient in absorbing metal elements from seawater.A 10-day simulation was conducted to assess the potential of scavenging cesium(Cs)and cobalt(Co)with live U lva prolifera that was cultured in seawater medium containing Cs and Co in diff erent concentrations(0(the control),10,20,50,and 100 mg/L).In the experiment,5 kg(fresh weight)of U.prolifera was cultured in natural seawater in 90-L tanks.Results showed that after the experiment,the average bioconcentration factors(BCFs)of the control group were 247.2 and 1126.4 for Cs and Co,respectively.The absolute absorption quantity of U.prolifera increased and the BCFs decreased with the increase in Cs and Co concentrations.The biosorption of the two metals on the fi rst day well fi t the pseudo-second-order equation(R^(2)>0.95),indicating that adsorption is the rate-limiting step in the total biosorption process.Concentrations of both metal ions declined signifi cantly in the fi rst hour and decreased by 25.2%and 15.5%in 48 h,respectively.Therefore,live U.prolifera is effi cient at scavenging Cs and Co in seawater,providing potential applications for the phytoremediation of radionuclides contaminated seawaters. | Xuemei WANG Tifeng SHAN Shaojun PANG | 2022 | Journal of Oceanology and Limnology2022，40，4： | 0 |
| 6 | Biological identification and determination of optimum growth conditions for four species of Navicula显示文摘Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy(SEM) and sequence comparisons of two segments of genes(small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm(OD680) under various environmental factors. Results showed that the optimum culture conditions were 25℃, 50–100 μmol photons m-2 s-1, pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates(RGR) peaked at the highest tested level(0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration. | ZHAO Xiaobo PANG Shaojun LIU Feng SHAN Tifeng LI Jing | 2014 | Acta Oceanologica Sinica2014，33，8： | 0 |
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