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| 1 | Six-fold speed-up of Smith-Waterman sequence database searches using parallel processing on common microprocessors显示文摘 | ROGES T SEEBERG E | | 0，，： | 1 |
| 2 | Formation of β-glucosamine and β-mannose linkages using glycosyl phosphates 显示文摘 | Plante O J Palmacci E R Seeberger P H | 2000 | Org Lett2000，2，24： | 1 |
| 3 | Troponin T and B-type natriuretic peptide after on-pump cardiac surgery:prognostic impact on 12-month mortality and major cardiac events after adjustment for postoperative complications显示文摘 | LURATI B G BOLLIGER D SEEBERGER E | 2014 | Circula- tion2014，130，12： | 1 |
| 4 | Automated solid-phase synthesis of oligosaccharides 显示文摘 | Plante O J Palmacci E R Seeberger P H | 2001 | Scienc2001，291，： | 1 |
| 5 | Six-fold speed-up of Smith Waterman sequence database searches using parallel processing on common microprocessors 显示文摘 | Rognes T Seeberg E | 2000 | Journal of Bioinformatics2000，16，8： | 1 |
| 6 | Progress toward developing a carbohydrate-conjugate vaccine against Clostrid ium difficile ribotype 027 : synthesis o the cell-surface polysaccharide PS-I repeating unit显示文摘 | Martin C E Weishaupt M W Seeberger P H | 2011 | Chemical Communications2011，47，10： | 1 |
| 7 | Microreactors as tools for synthetic chemists-the chemists' round-bottomed flask of the 21st century?显示文摘 | GEYER K CODéE J D SEEBERGER P H | 2007 | Chemistry--A European Journal2007，38，2： | 1 |
| 8 | Antimutator role of DNA glyeosylase MutY in pathogenic Neisseria species 显示文摘 | Davidsen T Bjoras M Seeberg E C | 2005 | J Bacteriol2005，187，8： | 1 |
| 9 | Randomizedcomparison of sevoilurane versus propofol to reduce perioperativemyocardial ischemia in patients undergoing noncardiac surgery显示文摘 | Lurati Buse GA Schumacher P Seeberger E | 2012 | Circulation2012，126，23： | 1 |
| 10 | AlkB-mediated oxidative demethylation reverses DNA damage in Eseherichia coli显示文摘 | Falnes Pφ Johansen RF Seeberg E | 2002 | Nature2002，419，6903： | 1 |
| 11 | Reciprocal 'flipping' underlies substrate recognition and catalytic activation by the human 8-oxo-guanine DNA glycosylase显示文摘 | SEEBERG E LUNA L | 2002 | J Mol Biol2002，317，： | 1 |
| 12 | Antimutator role of DNA glycosylase MutY in pathogenic Neisseria species 显示文摘 | Davidsen T Bjoras M Seeberg E C Tonjum T | 2005 | J Bacteriol2005，187，： | 1 |
| 13 | Randomized comparison of sevoflurane versus propofol to reduce perioperative myocardial ischemia in patients undergoing noncardiac surgery显示文摘 | Lurati Buse GA Schumacher P Seeberger E | 2012 | Circulation2012，126，23： | 1 |
| 14 | Six-fold speed-up of Smith-Waterman sequence database searches using parallel processing on common microprocessors Bioinformatics显示文摘 | Rognes T Seeberg E | 2000 | PubMed2000，16，8： | 1 |
| 15 | Microreactors as tools for synthetic chemists--the chemists′ round-bottomed flask of the 21st Century?显示文摘 | GEYER K COD(E)E J D C SEEBERGER P H | 2006 | Chemistry-A European Journal2006，12，33： | 1 |
| 16 | The base excision repair pathway显示文摘 | Seeberg E Eide L Bjoras M | 1995 | Trends Biochem Sci1995，20，10： | 1 |
| 17 | Ran- domized comparison of sevoflurane versus pmpofol to re- duce perioperative myocardial isehemia in patients under- going noncardiac surgery 显示文摘 | Lurati Buse GA Schumacher P Seeberger E | 2012 | Circulation2012，126，： | 1 |
| 18 | Randomized compari- son of sevoflurane versus propofol to reduce perioperative myocardial ischemia in patients undergoing noncardiac surgery 显示文摘 | Lurati BOA Schumacher P Seeberger E | 2012 | Circulation2012，126，23： | 1 |
| 19 | Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium显示文摘AIM:To minimize the expansion of pancreatic mesenchymal cells in vitro and confirm thatβ-cell progenitors reside within the pancreatic epithelium.METHODS:Due to mesenchymal stem cell(MSC)expansion and overgrowth,progenitor cells within the pancreatic epithelium cannot be characterized in vitro,thoughβ-cell dedifferentiation and expansion of MSC intermediates via epithelial-mesenchymal transition(EMT)may generateβ-cell progenitors.Pancreatic epithelial cells from endocrine and non-endocrine tissue were expanded and differentiated in a novel pancreatic epithelial expansion medium supplemented with growth factors known to support epithelial cell growth(dexamethasone,epidermal growth factor,3,5,3’-triiodo-l-thyronine,bovine brain extract).Cells were also infected with a single and dual lentiviral reporter prior to cell differentiation.Enhanced green fluorescent protein was controlled by the rat Insulin 1 promoter and the monomeric red fluorescent protein was controlled by the mouse PDX1 promoter.In combination with lentiviral tracing,cells expanded and differentiated in the pancreatic medium were characterized by flow cytometry(BD fluorescence activated cell sorting),immunostaining and real-time polymerase chain reaction(PCR)(7900HT Fast Realtime PCR System).RESULTS:In the presence of 10%serum MSCs rapidly expand in vitro while the epithelial cell population declines.The percentage of vimentin+cells increased from 22%±5.83%to 80.43%±3.24%(14 d)and99.00%±0.0%(21 d),and the percentage of epithelial cells decreased from 74.71%±8.34%to 26.57%±9.75%(14 d)and 4.00%±1.53%(21 d),P<0.01 for all time points.Our novel pancreatic epithelial expansion medium preserved the epithelial cell phenotype and minimized epithelial cell dedifferentiation and EMT.Cells expanded in our epithelial medium contained significantly less mesenchymal cells(vimentin+)compared to controls(44.87%±4.93%vs 95.67%±1.36%;P<0.01).During cell differentiation lentiviral reporting demonstrated that,PDX1+and insulin+cells were localized within adherent epithelial cell aggregates compared to controls.Compared to starting islets differentiated cells had at least two fold higher gene expression of PDX1,insulin,PAX4 and RFX(P<0.05).CONCLUSION:PDX1+cells were confined to adherent epithelial cell aggregates and not vimentin+cells(mesenchymal),suggesting that EMT is not a mechanism for generating pancreatic progenitor cells. | Karen L Seeberger Sarah J Anderson Cara E Ellis Telford Y Yeung Gregory S Korbutt | 2014 | World Journal of Diabetes2014，5，1： | 0 |
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