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| 1 | Single-cell transcriptomic analysis defines the interplay between tumor cells,viral infection,and the microenvironment in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an Epstein-Barr virus(EBV)-associated malignancy with a complex tumor ecosystem.How the interplay between tumor cells,EBV,and the microenvironment contributes to NPC progression and immune evasion remains unclear.Here we performed single-cell RNA sequencing on^104,000 cells from 19 EBV^+NPCs and 7 nonmalignant nasopharyngeal biopsies,simultaneously profiling the transcriptomes of malignant cells,EBV,stromal and immune cells.Overall,we identified global upregulation of interferon responses in the multicellular ecosystem of NPC.Notably,an epithelial–immune dual feature of malignant cells was discovered and associated with poor prognosis.Functional experiments revealed that tumor cells with this dual feature exhibited a higher capacity for tumorigenesis.Further characterization of the cellular components of the tumor microenvironment(TME)and their interactions with tumor cells revealed that the dual feature of tumor cells was positively correlated with the expression of co-inhibitory receptors on CD8^+tumor-infiltrating T cells.In addition,tumor cells with the dual feature were found to repress IFN-γproduction by T cells,demonstrating their capacity for immune suppression.Our results provide new insights into the multicellular ecosystem of NPC and offer important clinical implications. | Shanzhao Jin Ruoyan Li Ming-Yuan Chen Chao Yu Lin-Quan Tang Yan-Min Liu Jiang-Ping Li Yi-Na Liu Yi-Ling Luo Yifan Zhao Yu Zhang Tian-Liang Xia Shang-Xin Liu Qi Liu Guan-Nan Wang Rui You Jing-Yun Peng Jiang Li Feng Han Jianwei Wang Qiu-Yan Chen Li Zhang Hai-Qiang Mai Benjamin E.Gewurz Bo Zhao Lawrence S.Young Qian Zhong Fan Bai Mu-Sheng Zeng | 2020 | Cell Research2020，30，11： | 16 |
| 2 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 3 | Synthesis of layered double hydroxides/graphene oxide nanocomposite as a novel high-temperature CO_2 adsorbent显示文摘In this contribution, a novel high-temperature CO2 adsorbent consisting of Mg-Al layered double hydroxide(LDH) and graphene oxide(GO)nanosheets was prepared and evaluated. The nanocomposite-type adsorbent was synthesized based on the electrostatically driven self-assembly between positively charged Mg-Al LDH single sheet and negatively charged GO monolayer. The characteristics of this novel adsorbent were investigated using XRD, FE-SEM, HRTEM, FT-IR, BET and TGA. The results showed that both the CO2 adsorption capacity and the multicycle stability of LDH were increased with the addition of GO owing to the enhanced particle dispersion and stabilization. In particular, the absolute CO2 capture capacity of LDH was increased by more than twice by adding 6.54 wt% GO as support. GO appeared to be especially effective for supporting LDH sheets. Moreover, the CO2 capture capacity of the adsorbent could be further increased by doping with 15 wt%K2CO3. This work demonstrated a new approach for the preparation of LDH-based hybrid-type adsorbents for CO2 capture. | Junya Wang Xueyi Mei Liang Huang Qianwen Zheng Yaqian Qiao Ketao Zang Shengcheng Mao Ruoyan Yang Zhang Zhang Yanshan Gao Zhanhu Guo Zhanggen Huang Qiang Wang | 2015 | Journal of Energy Chemistry2015，24，2： | 4 |
| 4 | SPDEF suppresses head and neck squamous cell carcinoma progression by transcriptionally activating NR4A1显示文摘SAM pointed domain containing E26 transformation-specific transcription factor(SPDEF)plays dual roles in the initiation and development of human malignancies.However,the biological role of SPDEF in head and neck squamous cell carcinoma(HNSCC)remains unclear.In this study,the expression level of SPDEF and its correlation with the clinical parameters of patients with HNSCC were determined using TCGA-HNSC,GSE65858,and our own clinical cohorts.CCK8,colony formation,cell cycle analysis,and a xenograft tumor growth model were used to determine the molecular functions of SPDEF in HNSCC.ChIP-qPCR,dual luciferase reporter assay,and rescue experiments were conducted to explore the potential molecular mechanism of SPDEF in HNSCC.Compared with normal epithelial tissues,SPDEF was significantly downregulated in HNSCC tissues.Patients with HNSCC with low SPDEF mRNA levels exhibited poor clinical outcomes.Restoring SPDEF inhibited HNSCC cell viability and colony formation and induced G0/G1 cell cycle arrest,while silencing SPDEF promoted cell proliferation in vitro.The xenograft tumor growth model showed that tumors with SPDEF overexpression had slower growth rates,smaller volumes,and lower weights.SPDEF could directly bind to the promoter region of NR4A1 and promoted its transcription,inducing the suppression of AKT,MAPK,and NF-κB signaling pathways.Moreover,silencing NR4A1 blocked the suppressive effect of SPDEF in HNSCC cells.Here,we demonstrate that SPDEF acts as a tumor suppressor by transcriptionally activating NR4A1 in HNSCC.Our findings provide novel insights into the molecular mechanism of SPDEF in tumorigenesis and a novel potential therapeutic target for HNSCC. | Yanting Wang Xianyue Ren Weiyu Li Ruoyan Cao Suyang Liu Laibo Jiang Bin Cheng Juan Xia | 2021 | International Journal of Oral Science2021，13，4： | 3 |
| 5 | Phylogenetic Analysis of the Dengue Virus Strains Causing the 2019 Dengue Fever Outbreak in Hainan,China显示文摘Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp.mosquitos,causing the acute infectious disease,dengue fever(DF).Until 2019,no dengue outbreak had been reported in Hainan Province for over 20 years.However,in early September of 2019,an increasing number of infected cases appeared and the DF outbreak lasted for over one month in Haikou City,Hainan Province.In our study,we collected 97 plasma samples from DF patients at three hospitals,as well as 1585 mosquito larvae samples from puddles in different areas of Haikou.There were 49(50.5%)plasma samples found to be strongly positive and 9(9.3%)plasma samples were weakly positive against the NS1 antigen.We discovered DENV both in the patient's plasma samples and mosquito larvae samples,and isolated the virus from C6/36 cells inoculated with the acute phase serum of patients.Phylogenetic analysis revealed that the new strains were the most closely related to the epidemic strain in the southern regions of China,belonging to lineageⅣ,genotypeⅠ,DENV-1.Compared to the seven closest strains from neighboring countries and provinces,a total of 18 amino acid mutations occurred in the coding sequences(CDS)of the new isolated strain,DENV1 HMU-HKU-2.Our data shows that dengue virus is re-emerged in Hainan,and pose new threats for public health.Thus regular molecular epidemiological surveillance is necessary for control and prevention of DENV transmission. | Jiang Du Liyuan Zhang Xiaoyuan Hu Ruoyan Peng Gaoyu Wang Yi Huang Wenqi Wang Kunliang Wu Qiang Wang Haoxiang Su Fan Yang Yun Zhang Chuanning Tang Xiuji Cui Lina Niu Gang Lu Meifang Xiao Yongguo Du Feifei Yin | 2021 | Virologica Sinica2021，36，4： | 2 |
| 6 | Investigation of health care waste management in Binzhou District, China显示文摘 | Ruoyan G Lingzhong X Huijuan L | 2010 | Waste Man- ag2010，30，2： | 1 |
| 7 | The antipsychotic aripiprazole is a potent, partial agonist at the human 5-HT1A re- ceptor显示文摘 | Jordan S Koprivica V Chen Ruoyan | 2002 | Eur J Pharmacol2002，441，3： | 1 |
| 8 | The role of vil- lage doctors on tuberculosis control and the DOTS strategy in Shandong Province, China显示文摘 | ] Ruoyan Gai Lingzhong Xu Xingzhou Wang | 2008 | BioScience Trends2008，2，5： | 1 |
| 9 | The antipsychotic aripiprazole is a potent, partial agonist at the human 5-HT 1A receptor显示文摘 | Shaun Jordan Vuk Koprivica Ruoyan Chen Katsura Tottori Tetsuro Kikuchi C.Anthony Altar | 2002 | European Journal of Pharmacology2002，，3： | 1 |
| 10 | Metagenomic insights into responses of microbial population and key functional genes to fulvic acid during partial nitritation显示文摘The long-term impact of fulvic acid(FA)on partial nitritation(PN)systemwas initially examined in this study.The obtained results revealed that the FA lower than 50 mg/L had negligible effect on the nitrite accumulation rate(NAR nearly 100%)and ammonium removal rate(ARR 56.85%),while FA over 50 mg/L decreased ARR from 56.85%to 0.7%.Sludge characteristics analysis found that appropriate FA(<50 mg/L)exposure promoted the settling performance and granulation of PN sludge by removing Bacteroidetes and accumulating Chloroflexi.The analysis of metagenomics suggested that the presence of limited FA(0-50 mg/L)stimulated the generation of NADH,which favors the denitrification and nitrite reduction.The negative impact of FA on the PN system could be divided into two stages.Initially,limited FA(50-120 mg/L)was decomposed by Anaerolineae to stimulate the growth and propagation of heterotrophic bacteria(Thauera).Increasing heterotrophs competed with AOB(Nitrosomonas)for dissolved oxygen,causing AOB to be eliminated and ARR to declined.Subsequently,when FA dosage was over 120 mg/L,Anaerolineae were inhibited and heterotrophic bacteria reduced,resulting in the abundance of AOB recovered.Nevertheless,the ammonium transformation pathway was suppressed because genes amoABC and hao were obviously reduced,leading to the deterioration of reactor performance.Overall,these results provide theoretical guidance for the practical application of PN for the treatment of FA-containing sewage. | Li Zhang Shuang Lan Quanhao Dou Shiwei Hao Yueping Wang Xiaoxuan Wang Ruoyan Zhang Yongzhen Peng Jiachun Yang | 2023 | Journal of Environmental Sciences2023，，2： | 1 |
| 11 | The antipsychotic aripiprazole is a potent,partial agonistat the human 5-HTIA receptor显示文摘 | Shaun J Vuk K Ruoyan C | 2002 | Euro-pean journal of Pharmacology2002，441，： | 1 |
| 12 | The antipsychotic aripiprazole is a potent,partial agonistat the human 5-HT1A receptor显示文摘 | SHAUN J VUK K RUOYAN C | 2002 | Eur J Pharmacol2002，36，441： | 1 |
| 13 | Simultaneous detection of ethyl carbamate and urea in chinese yellow rice wine by HPLC-FLD显示文摘 | WANG Ruoyan WU Hongmiao ZHOU Xiaoping | 2014 | Journal of Liquid Chromatography and Related Technologies2014，37，： | 1 |
| 14 | A“T.E.S.T.”hydrogel bioadhesive assisted by corneal cross-linking for in situ sutureless corneal repair显示文摘Corneal transplantation is an effective clinical treatment for corneal diseases,which,however,is limited by donor corneas.It is of great clinical value to develop bioadhesive corneal patches with functions of“Transparency”and“Epithelium&Stroma generation”,as well as“Suturelessness”and“Toughness”.To simultaneously meet the“T.E.S.T.”requirements,a light-curable hydrogel is designed based on methacryloylated gelatin(GelMA),Pluronic F127 diacrylate(F127DA)&Aldehyded Pluronic F127(AF127)co-assembled bi-functional micelles and collagen type I(COL I),combined with clinically applied corneal cross-linking(CXL)technology for repairing damaged cornea.The patch formed after 5 min of ultraviolet irradiation possesses transparent,highly tough,and strongly bio-adhesive performance.Multiple cross-linking makes the patch withstand deformation near 600%and exhibit a burst pressure larger than 400 mmHg,significantly higher than normal intraocular pressure(10-21 mmHg).Besides,the slower degradation than GelMA-F127DA&AF127 hydrogel without COL I makes hydrogel patch stable on stromal beds in vivo,supporting the regrowth of corneal epithelium and stroma.The hydrogel patch can replace deep corneal stromal defects and well bio-integrate into the corneal tissue in rabbit models within 4 weeks,showing great potential in surgeries for keratoconus and other corneal diseases by combining with CXL. | Meiyan Li Ruoyan Wei Chang Liu Haowei Fang Weiming Yang Yunzhe Wang Yiyong Xian Kunxi Zhang Yong He Xingtao Zhou | 2023 | Bioactive Materials2023，，7： | 1 |
| 15 | Late Jurassic-Early Cretaceous tectonic switching in Liaodong Peninsula of the North China Craton and the implications for gold mineralisation显示文摘Constraining the processes of deformation during tectonic switching from compression to extension is difficult because of the scarcity or absence of associated sedimentary and magmatic rocks and weak metamorphism.The east margin of the eastern North China Craton experienced Early to Middle Jurassic compression and Early Cretaceous extension.However,the period of tectonic quiescence lasting~13 million years(between 153 and 140 Ma)during which this transition occurred is poorly understood.This paper reports the identification of small-scale N-S-trending thrust and sinistral strike-slip faults(TSS)and NWtrending thrust and dextral strike-slip faults(TDS)in the Tongyuanpu-Aiyang region,which is part of the northern Liaodong Peninsula.Calculation of the tectonic stress field using striations,fault planes and kinematics reveals a NW-SE-oriented maximum principal axis(σ1),and sub-horizontalσ2 andσ3.Assemblages of N-S-trending TSS and NW-trending TDS were arranged with right stepping and resulted in local uplift and erosion at the junctions between fault terminations,which partitioned the Early and Middle Jurassic basins into residual smaller volcanic-sedimentary basins.These basins were unconformably overlain by small Early Cretaceous volcanic-sedimentary basins,such as those at Tongyuanpu and Fangjiaweizi.Magmatic ages and the timing of basin formation constrain the initiation of tectonic switching to 156–153 Ma,and its termination to 140–139 Ma.We suggest that R-R’Riedel shears controlled the formation of the N-S-trending TSS and NW-trending TDS.The R-R’shears were produced by continued sinistral strike-slip and northward growth along the Bohai Bay segment of the Tan-Lu fault zone during the Late Jurassic to early Early Cretaceous,simultaneous with a gradual weakening in the tectonic stress field during a switch in the direction of subduction of the Palaeo-Pacific plate from NW-ward to NNW-ward.This tectonic switching might have promoted the dissolution of gold and migration of gold-bearing fluids. | Dan-Ping YAN Ruoyan KONG Xiaoyu DONG Liang QIU Huilong LIU | 2021 | Science China Earth Sciences2021，64，9： | 1 |
| 16 | The antipsychotic aripiprazole is a potent,partial agonistat the human 5-HT1A receptor显示文摘 | Shaun J Vuk K Ruoyan C | 2002 | European Journal of Pharmacology2002，441，： | 1 |
| 17 | Ontology-driven web services composition platform显示文摘 | Budak Arpinar Zhang Ruoyan Boanerges Aleman-Mezaetal | 2005 | Information Systems and E2Business Management2005，3，2： | 1 |
| 18 | The antipsychotic aripiprazole is a po- tent,partial agonistat the human 5-HT1A receptor显示文摘 | Shaun J Vuk K Ruoyan C | 2002 | European Journal of Pharmacology2002，441，： | 1 |
| 19 | The management of hepatocellular carcinoma around the world: a comparison of guidelines from 2001 to 2011显示文摘 | Peipei Song Ruoyan G. Tobe Yoshinori Inagaki Norihiro Kokudo Kiyoshi Hasegawa Yasuhiko Sugawara Wei Tang | 2012 | Liver Int2012，，7： | 1 |
| 20 | The antipsychoticaripiprazole is a potent, partial agonist at the human 5-HT1A receptor显示文摘 | Jordan S Koprivica V Chen Ruoyan | 2002 | Eur J Pharmacol2002，441，3： | 1 |
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