| 1 | Optimal dietary copper requirements and relative bioavailability for weanling pigs fed either copper proteinate or tribasic copper chloride显示文摘Background: The objective of this study was to determine the effects of supplementing Cu on growth performance, Cu metabolism and Cu-related enzyme activities of weanling pigs fed diets with two different Cu sources, and to estimate optimal Cu requirements and relative bioavailability from these two sources for pigs.Methods: Weanling pigs were allocated to 14 treatments arranged factorially, including 6 added Cu levels(5, 10,20, 40, 80, 160 mg/kg), and 2 mineral sources(tribasic Cu chloride, TBCC and copper proteinate, Cu Pro), as well as one negative control(0 mg/kg added Cu level) and one maximum allowed level treatment(200 mg/kg TBCC) for the entire 38-d experiment. Growth performance, mineral status and enzyme activities were measured at the end of this study.Results: Increasing levels of Cu showed linear and quadratic responses(P < 0.01) for final BW, ADG and FCR regardless of the sources. Supplementation with TBCC(> 80 mg/kg) and Cu Pro(> 20 mg/kg) significantly decreased(P < 0.05) diarrhea incidence of weanling pigs. There were linear and quadratic increases(P < 0.01) in bile, hepatic,and intestinal Cu concentrations, fecal Cu contents, and plasma enzyme activities(alkaline phosphatase,ceruloplasmin, Cu, Zn-Superoxide dismutase(Cu/Zn SOD), and glutathione peroxidase), whereas plasma malondialdehyde decreased(P < 0.01) linearly and quadratically as dietary Cu level increased. Similarly, pigs fed Cu Pro absorbed and retained more Cu and excreted less Cu than those fed TBCC when supplemented 80 mg/kg and above. Optimal dietary Cu requirements for pigs from 28 to 66 d of age estimated based on fitted broken-line models(P < 0.05) of bile Cu, plasma Cu/Zn SOD and growth performance were 93–140 mg/kg from TBCC, and 63–98 mg/kg from Cu Pro accordingly. According to slope ratios from multiple linear regression, the bioavailability value of Cu Pro relative to TBCC(100%) was 156–263%(P < 0.01).Conclusion: The findings indicated that Cu recommendation from current NRC(5–6 mg/kg) was not sufficient to meet the high requirement of weanling pigs. Cu from Cu Pro was significantly more bioavailable to weanling pigs than TBCC in stimulating growth and enzyme activities, decreasing diarrhea frequency and fecal Cu contents to the environment. | Gang Lin Yang Guo Bing Liu Ruiguo Wang Xiaoou Su Dongyou Yu Pingli He | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 5 |
| 2 | Identification of a Differentially-expressed Gene in Fatty Liver of Overfeeding Geese显示文摘响应 overfeeding,鹅得脂肪肝。理解肥育的机制, mRNA 微分显示反转录 PCR 被用来处于 overfeeding 并且正常的喂的条件学习法语的 Landes 灰鹅和 Xupu 白鹅之间的基因表达差别。一基因被发现在在两个品种的脂肪肝起来调整,并且它与83%身份有 1797 bp cDNA 到揭示的鸡 SELENBP1.The 顺序分析它 1413 bp 的开的读物框架编码 471 氨基酸的蛋白质,它与高相同包含 56 kDa 硒绑定蛋白质的通常认为的保存的域到它鸡肉(95%)的相当或相同事物,老鼠(86%),老鼠(84%),人(86%),猴子(86%),狗(86%),并且这蛋白质的牛(86%) .Thefunction 简短基于出版信息被考察了。在织物表达式分析,鹅 SELENBP1 mRNA 的表达式被发现比在另外的测试纸巾在肝或肾更高。结果证明 overfeeding 能增加鹅 SELENBP1 的 mRNA 表达式水平。 | Ayong ZHAO Huachun TANG Sufang LU Ruiguo HE | 2007 | Acta Biochimica et Biophysica Sinica2007，39，9： | 4 |
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