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您的检索式：作者名="Romit Seth"
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| 1 | Underpinning the molecular programming attributing heat stress associated thermotoleranee in tea (Camellia sinensis (L.) O. Kuntze)显示文摘The most daunting issue of global climate change is the deleterious impact of extreme temperatures on tea productivity and quality,which has resulted in a quest among researchers and growers.The current study aims to unravel molecular programming underpinning thermotolerance by characterizing heat tolerance and sensitivity response in 20 tea cultivars.The signi fi cantly higher negative in fl uence of heat stress was recorded in a sensitive cultivar with reduced water retention(47%),chlorophyll content(33.79%),oxidation potential(32.48%),and increase in membrane damage(76.4%).Transcriptional pro fi ling of most tolerant and sensitive cultivars identi fi ed 78 differentially expressed unigenes with chaperon domains,including low and high molecular weight heat shock protein(HSP)and heat shock transcription factors(HSFs)involved in heat shock response(HSR).Further,predicted transcriptional interactome network revealed their key role in thermotolerance via well-co-ordinated transcriptional regulation of aquaporins,starch metabolism,chlorophyll biosynthesis,calcium,and ethylene mediated plant signaling system.The study identi fi ed the key role of HSPs(CsHSP90)in regulating HSR in tea,wherein,structure-based molecular docking revealed the inhibitory role of geldanamycin(GDA)on CsHSP90 by blocking ATP binding site at N-terminal domain of predicted structure.Subsequently,GDA mediated leaf disc inhibitor assay further af fi rmed enhanced HSR with higher expression of CsHSP17.6,CsHSP70,HSP101,and CsHSFA2 genes in tea.Through the current study,efforts were made to extrapolate a deeper understanding of chaperons mediated regulation of HSR attributing thermotolerance in tea. | Romit Seth Tony Kipkoech Maritim Rajni Parmar Ram Kumar Sharma | 2021 | Horticulture Research2021，8，1： | 0 |
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