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您的检索式：作者名="Rithwick Rajagopal"
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| 1 | Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy显示文摘AIM:To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy.METHODS:Un-masked cross-sectional series of 34 eyes undergoing vitrectomy.Vitreous specimens were collected and processed to evaluate for membrane integrity(DAPI),apoptosis(Annexin-V),and endothelial-cell origin(V-Cadherin).A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size comparison.RESULTS:Thirty-four specimens underwent analysis.Greater levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous(n=12)compared to those without blood(n=22;52.3%±30.7%vs 19.6%±27.2%,P=0.002).Patients with diabetes having surgery with hemorrhage(n=7)had greater expression of Annexin-V than those without hemorrhage(n=8;62.1%±31.7%vs 18.9%±20.9%,P=0.009).However,in patients with non-diabetic vitreous hemorrhage,the level of Annexin-V expression was not significantly different compared to other disease processes(38.6%±25.7%,n=5 vs 20.0%±30.9%,n=14,P=0.087).CONCLUSION:Increased expression of the apoptotic marker,Annexin-V is detected on vitreous microparticles in diabetes-related vitreous hemorrhage.When evaluating vitreous hemorrhage in patients without diabetes,the apoptotic signal is not significantly different.Vitrectomy in patients with diabetes,and improvement in visual outcomes,may be related to the removal of a serumderived,pro-apoptotic vitreous.Further investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease. | Harris Sultan Rithwick Rajagopal Prabakar Kumar Rao Kisha Deslee Piggott Michael A Paley Lynn Marisa Hassman Albert S.Li Brigid Marshall Rajendra Shridhar Apte | 2022 | International Journal of Ophthalmology(English edition)2022，15，1： | 0 |
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